Agilent XF PDL User Manual

Agilent Seahorse XF PDL-Coated Cell Culture Microplates

Ready-to-use precoated XF microplates for consistent XF assay results

Poly-D-Lysine (PDL) is a synthetic molecule that enhances cell adhesion to solid substrate by increasing the number of positively charged cell binding sites. This leads to increased electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. PDL is required when performing XF assays with nonadherent cell types (e.g., T cells) and can enhance binding of loosely adherent cell types (e.g., neurons). PDL has been shown to support growth and survival of many centralnervous-system primary cells in culture.

Agilent now offers ready-to-use XF PDL-coated cell culture microplates in 96-well and 8-well formats. These products provide convenience by eliminating the time and labor involved in manually coating microplates. They also reduce data variation commonly caused by a manual coating processes. For information on how to use XF PDL-coated cell culture microplates, please refer to the Agilent Cell

Analysis Learning Center or contact Technical Support.

Product ordering information

Part Number Product Description Compatible Analyzer

103729-100 Seahorse XFe96 FluxPak Mini (PDL Plates), 6 assays XFe96/XF96 Analyzers

103730-100 Seahorse XFe96 PDL Cell Culture Plates, 6 assays XFe96/XF96 Analyzers

103721-100 Seahorse XFp FluxPak (PDL Plates) XF HS Mini, XFp Analyzers

103722-100 Seahorse XFp PDL Cell Culture Miniplates XF HS Mini, XFp Analyzers

103724-100 Seahorse XF HS Mini FluxPak (PDL plates) XF HS Mini Analyzer

103727-100 Seahorse XF HS PDL Miniplates XF HS Mini Analyzer

Storage Requirements:

Room temperature (4C-30C) for 1 year from the date of manufacturing.

Learn more

www.agilent.com/chem/discoverXF

For Research Use Only. Not for use in diagnostic procedures.

This information is subject to change without notice.

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