Agilent InfinityLab LC User Manual

Download

Vialsamplers

Agilent InfinityLab LC Series

User Manual

Agilent InfinityLab LC Series Vialsamplers User Manual

Notices

CAUTION

WARNING

Document Information

Document No: SD-29000242 Rev. E Part No: G7129-90010 Rev. E EDITION 04/2020

No part of this manual may be reproduced in any form or by any means (including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent from Agilent Technologies, Inc. as governed by United States and international copyright laws.

Agilent Technologies Hewlett-Packard-Strasse 8 76337 Waldbronn

Warranty

The material contained in this document is provided “as is,” and is subject to being changed, without notice, in future editions. Further, to the maximum extent permitted by applicable law, Agilent disclaims all warranties, either express or implied, with regard to this manual and any information contained herein, including but not limited to the implied warranties of merchantability and fitness for a particular purpose. Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing, use, or performance of this document or of any information contained herein. Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms, the warranty terms in the separate agreement shall control.

Technology Licenses

The hardware and/or software described in this document are furnished under a license and may be used or copied only in accordance with the terms of such license.

Safety Notices

CAUTION

hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a the indicated conditions are fully understood and met.

A WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in personal injury or death. Do not proceed beyond a WARNING notice until the indicated conditions are fully understood and met.

notice denotes a

CAUTION

notice until

Restricted Rights Legend

U.S. Government Restricted Rights. Software and technical data rights granted to the federal government include only those rights customarily provided to end user customers. Agilent provides this customary commercial license in Software and technical data pursuant to FAR

12.211 (Technical Data) and 12.212 (Computer Software) and, for the Department of Defense, DFARS 252.227-7015 (Technical Data - Commercial Items) and DFARS 227.7202-3 (Rights in Commercial Computer Software or Computer Software Documentation).

Agilent InfinityLab LC Series Vialsamplers User Manual

In This Book:

This manual covers the following modules:

• Agilent 1260 Infinity II Vialsampler (G7129A)

• Agilent 1290 Infinity II Vialsampler (G7129B)

• Agilent 1260 Infinity II Vialsampler (G7129C)

1Introduction

This chapter gives an introduction to the module and instrument overview.

2 Site Requirements and Specifications

This chapter provides information on environmental requirements, physical and performance specifications.

3 Using the Module

This chapter provides information on how to use the module.

4 Preparing the Module

This chapter explains the operational parameters of the module.

5 Optimizing Performance

This chapter provides information on how to optimize the module.

6Troubleshooting and Diagnostics

This chapter gives an overview of the maintenance, troubleshooting, and diagnostic features available for the Agilent InfinityLab Series Vialsampler.

7Error Information

This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions.

Agilent InfinityLab LC Series Vialsamplers User Manual 3

8 Maintenance

This chapter describes the maintenance of the module.

9 Parts and Materials for Maintenance

This chapter provides information on parts for maintenance.

10 Identifying Cables

This chapter provides information on cables used with the Agilent 1200 Infinity Series modules.

11 Hardware Information

This chapter describes the detector in more detail on hardware and electronics.

12 LAN Configuration

This chapter provides information on connecting the module to the Agilent ChemStation PC.

13 Appendix

This chapter provides additional information on safety, legal and web.

Agilent InfinityLab LC Series Vialsamplers User Manual 4

Contents

1Introduction 9

Product Description (G7129A) 10 Features (G7129A) 11 Product Description (G7129B) 12 Features (G7129B) 13 Product Description (G7129C) 14 Features (G7129C) 15 Overview of the Module 16 Operating Principle 18 Leak and Waste Handling 27

2 Site Requirements and Specifications 33

Site Requirements 34 Physical Specifications 38 Performance Specifications 39 Specifications of the Sample Cooler 47 Specifications of the Sample Thermostat 49 Specifications of the Integrated Column Compartment 51

3 Using the Module 53

Magnets 55 Turn on/off 56 Status Indicators 58 Vial Drawers and Trays 59 Choice of Vials and Caps 70 Install the Optional Integrated Column Compartment 74 Using the Optional Integrated Column Compartment 96 Install the Optional Sample Cooler/Sample Thermostat 116 Using the Optional Sample Cooler/Sample Thermostat 123 Transporting the Sampler 132 Agilent Local Control Modules 137

Agilent InfinityLab LC Series Vialsamplers User Manual 5

4 Preparing the Module 139

Leak and Waste Handling 140 Preparing the Module 141 Solvent Information 142 Capillary Color Coding Guide 149 Installing Capillaries 150 Flow Connections to the Vialsampler 152 Setting up the Vialsampler 154

5 Optimizing Performance 166

Optimization for Lowest Carryover 167 Fast Injection Cycle and Low Delay Volume 171 Precise Injection Volume 173 Choice of Rotor Seal 175

6 Troubleshooting and Diagnostics 176

Status Indicators 177 Overview of Tests and Tools 178 Maintenance and Troubleshooting Tools 179 Diagnostic Tests 187 Agilent Lab Advisor Software 189

7Error Information 190

What are Error Messages 192 General Error Messages 193 Vialsampler Error Messages 201 Sample Cooler/Sample Thermostat Error Messages 213 Integrated Column Compartment (ICC) Heater Error Messages 219

Agilent InfinityLab LC Series Vialsamplers User Manual 6

8 Maintenance 222

Introduction to Maintenance 223 Warnings and Cautions 224 Overview of Maintenance 226 Cleaning the Module 227 Remove and Install Doors 228 Exchange the Needle Assembly 229 Exchange the Needle Seat Assembly 235 Exchange the Sample Loop Assembly 241 Exchange the Rotor Seal 245 Exchange the Metering Seal and Piston 250 Replace the Analytical Heads 255 Exchange the Gripper Arm 258 Replace the Peristaltic Pump Cartridge 261 Exchange the Wash Port Assembly 263 Replace the Module Firmware 269 Replace the Sample Cooler/Sample Thermostat 270

9 Parts and Materials for Maintenance 275

Standard Parts For Maintenance 276 Accessory Kits 279 Vial Drawers and Trays 283 Multidraw Kits 286 Sample Thermostat Upgrade 287 Integrated Column Compartment 288 Column ID Upgrade Kit 289 Parts for 900 µL Injection Upgrade 290 Cabinet and Door Kits 291 Analytical Head Assembly (100 µL) 295 Analytical-Head Assembly (900 µL) 296 Analytical Head Assembly (40 μL) 297 2 Position/6 Port Injection Valve, 600 bar 298 2 Position/6 Port Injection Valve, 800 bar 299 2 Position/6 Port Injection Valve, 1300 bar 300

Agilent InfinityLab LC Series Vialsamplers User Manual 7

10 Identifying Cables 301

Cable Overview 302 Analog Cables 304 Remote Cables 306 CAN/LAN Cables 310 RS-232 Cables 311 USB 312

11 Hardware Information 313

Firmware Description 314 Electrical Connections 317 Interfaces 319 Setting the 6-bit Configuration Switch 326 Instrument Layout 330 Early Maintenance Feedback (EMF) 331

12 LAN Configuration 332

What You Have to Do First 333 TCP/IP parameter configuration 334 Configuration Switches 335 Initialization Mode Selection 336 Dynamic Host Configuration Protocol (DHCP) 338 Manual Configuration 341 PC and Agilent ChemStation Setup 345

13 Appendix 354

General Safety Information 355 Waste Electrical and Electronic Equipment (WEEE) Directive 361 Refrigerant 362 Radio Interference 365 Sound Emission 366 Solvent Information 367 Agilent Technologies on Internet 368

Agilent InfinityLab LC Series Vialsamplers User Manual 8

1Introduction

Sampling Sequence 18 Needle Parkstation 23 Hydraulic Box 24 Transport Assembly 26

Leak and Waste Handling 27

Leak Sensor 31 Waste Guidance 31 Waste Concept 32

This chapter gives an introduction to the module and instrument overview.

Agilent InfinityLab LC Series Vialsamplers User Manual 9

1Introduction

Status indicator

Needle assembly

Drawer

Column shelf

Leak drain

Power switch

Product Description (G7129A)

The Agilent 1260 Infinity II Vialsampler is an autosampler designed for the reliability and ease-of-use needed for routine pharmaceutical tasks and quality control, as well as for environmental and food analyses. It can house optionally the integrated column compartment for two LC columns with temperature control up to 80 4

°C to 40 °C, all within one module.

°C as well as a sample thermostat for stable temperatures from

Agilent InfinityLab LC Series Vialsamplers User Manual 10

Figure 1 Overview of the Vialsampler

1Introduction

Features (G7129A)

• High capacity – up to 132 vials (2 mL) or up to 36 vials (6 mL).

• Reliable injections – 0.1 to 100 µL injections for up to 600 bar (G7129A) and

up to 800

• Easy volume extensions – for injection volumes up to 1800 µL for applications ranging from microbore to semipreparative chromatography.

•Lowest carryover – with an in needle flush port included, for rinsing the outside of the needle.

• Efficient temperature control – with an integrated column compartment as option or upgrade available. Which holds two columns up to 30 and provides heating capacity from 5 reproducible chromatography data at optimized resolution.

• Integrated sample thermostat – available as option or upgrade, providing cooling and heating in the range from 4

• Low internal volume – for minimum contribution to a system's total internal volume, which can be even further reduced using "bypass" mode.

•Increased productivity – with overlapped injections.

• Customizable injection program – available for customizing advanced

injections as well as for sample preparation steps upfront injection.

bar (G7129C).

cm length,

°C above ambient up to 80 °C for

°C - 40 °C.

Agilent InfinityLab LC Series Vialsamplers User Manual 11

1Introduction

Status indicator

Needle assembly

Drawer

Column shelf

Leak drain

Power switch

Product Description (G7129B)

The Agilent 1290 Infinity II Vialsampler (G7129B) is an autosampler designed for UHPLC applications up to 1300 ease-of-use needed for routine pharmaceutical tasks and quality control, as well as for environmental and food analyses. It can house optionally the integrated column compartment for two LC columns with temperature control up to 80 as well as a sample thermostat for stable temperatures from 4 within one module.

bar. It provides the reliability, safety, and

°C

°C to 40 °C, all

Agilent InfinityLab LC Series Vialsamplers User Manual 12

Figure 2 Overview of the Vialsampler

1Introduction

Features (G7129B)

• Accurate and precise injections - within a wide and flexible range of volumes

• High capacity - up to 132 vials (2 mL) or up to 36 vials (6 mL).

• Easy volume extensions - for injection volumes up to 1500 µL for applications

ranging from microbore to semipreparative chromatography.

•Lowest carryover - with an in needle flush port included, for rinsing the outside of the needle.

• Efficient temperature control - with an integrated column compartment as option or upgrade available. Which holds two columns up to 30 and provides heating capacity from 5 reproducible chromatography data at optimized resolution.

• Integrated sample thermostat - available as option or upgrade, providing cooling and heating in the range from 4

• Low internal volume - for minimum contribution to a system's total internal volume, which can be even further reduced using bypass mode.

•Increased productivity - with overlapped injections.

• Customizable Injection program - available for customizing advanced

injections as well as for sample preparation steps upfront injection.

°C above ambient up to 80 °C for

– 40 °C.

cm length,

Agilent InfinityLab LC Series Vialsamplers User Manual 13

1Introduction

Status indicator

Needle assembly

Drawer

Column shelf

Leak drain

Power switch

Product Description (G7129C)

°C to 40 °C, all within one module.

°C as well as a sample thermostat for stable temperatures from

Agilent InfinityLab LC Series Vialsamplers User Manual 14

Figure 3 Overview of the Vialsampler

1Introduction

Features (G7129C)

• High capacity – up to 132 vials (2 mL) or up to 36 vials (6 mL).

• Reliable injections – 0.1 to 100 µL injections for up to 600 bar (G7129A) and

up to 800

• Easy volume extensions – for injection volumes up to 1800 µL for applications ranging from microbore to semipreparative chromatography.

•Lowest carryover – with an in needle flush port included, for rinsing the outside of the needle.

• Integrated sample thermostat – available as option or upgrade, providing cooling and heating in the range from 4

• Low internal volume – for minimum contribution to a system's total internal volume, which can be even further reduced using "bypass" mode.

•Increased productivity – with overlapped injections.

• Customizable injection program – available for customizing advanced

injections as well as for sample preparation steps upfront injection.

bar (G7129C).

cm length,

°C above ambient up to 80 °C for

°C - 40 °C.

Agilent InfinityLab LC Series Vialsamplers User Manual 15

1Introduction

Overview of the Module

The Agilent InfinityLab LC Series Vialsampler is designed for use with other modules of the Agilent InfinityLab LC Series, 1200 Series, and 1100 Series LC, as well as with other LC systems with adequate remote-control capabilities. The Vialsampler can be controlled from any computer with a suitable chromatography data system (for example, Agilent OpenLab CDS, MassHunter, etc.) or via an Agilent local control module (InfinityLab Companion or 1200 Infinity Series Instant Pilot).

The 1290 Infinity II Vialsampler (G7129B) can be operated at up to 1300 bar. The two 1260 Infinity II Vialsampler variants (G7129A and G7129C) are limited to a maximum system pressure of 600 and 800

The Vialsampler can accommodate two vial drawers, providing space for up to 132 samples. The two cartesian vial drawers allow for the use of either 66 × 2 or 18 × 6 system is also available, each capable of hosting 50 × 2 drawers are designed to ease method transfer from older Agilent samplers. The Vialsampler can also be equipped with an external tray, which can be beneficial for automation. The Agilent WalkUp solution fully supports the external tray.

mL vials. A pair of classic vial drawers with a numerical vial assignment

bar, respectively.

mL vials. The classic vial

The Vialsampler features a robotic gripper arm for the transport of vials, driven by four stepper motors to ensure the highest precision and flexibility for the transport movement.

The default configuration of the analytical head and sample loop allows an injection range from 0.1

0.1

– 100 μL for the 1260 Infinity II Vialsamplers. An analytical head with extended volume is also available for injection volumes from 0.1 can be operated at up to 400 volume can even reach 1800

The 2-position/6-port injection valve is driven by a high-speed, hybrid stepper motor, ensuring smooth switching between the mainpass and bypass positions. Thanks to the flow-through design of the Vialsampler, the eluent continuously rinses the component parts of the sample introduction system. This, together with the automated needle wash function for rinsing the outer surface of the needle, ensures the lowest level of carryover for each analysis. For advanced delay volume reduction, the injection valve can be programmed to automatically switch back to the bypass position when the injection is completed.

Agilent InfinityLab LC Series Vialsamplers User Manual 16

– 20 μL for the 1290 Infinity II Vialsampler and

– 900 μL, and bar. With the Multidraw Kit, the maximum injection μL.

1Introduction

Overview of the Module

The Agilent InfinityLab Integrated Column Compartment, an optional upgrade for the Vialsampler, eliminates the need for having a standalone column thermostat in the LC stack. It has the heating capability of reaching temperatures up to 80 and can hold a total of two columns with maximum lengths of 30

For applications requiring control over the vial temperature, the Vialsampler can be equipped with the Agilent InfinityLab Sample Thermostat (G7167-60101). It features a vapor-compression refrigeration system and an electric heater, allowing the Vialsampler to reach vial temperatures down to 4

cm.

°C and up to 40 °C.

°C

Agilent InfinityLab LC Series Vialsamplers User Manual 17

1Introduction

Operating Principle

Sampling Sequence

The Vialsampler processor continuously monitors the movements of the vialsampler components during the sampling sequence. The processor defines specific time windows and mechanical ranges for each movement. If a specific step of the sampling sequence can’t be completed successfully, an error message is generated.

During the sampling sequence, the solvent bypasses the vialsampler via the injection valve. The gripper arm selects the sample vial, either from a static sample rack, or from external vial positions. The gripper arm places the sample vial below the injection needle. The required volume of sample is drawn into the sample loop by the metering device. Sample is applied to the column when the injection valve returns to the mainpass (main path) position at the end of the sampling sequence.

The sampling sequence occurs in the following order:

1 The injection valve switches to the bypass position. 2 The piston of the metering device moves to the initialization position. 3 The gripper arm moves from the home position, and selects the vial. At the

same time, the needle lifts out of the seat.

4 The gripper moves into the needle station and stops in the draw position. 5 The needle lowers into the vial. 6 The metering device draws the defined sample volume. 7 The needle lifts out of the vial.

8 The gripper arm moves out slightly and stops in the wash position1. 9 The needle moves downwards and dips into the wash well of the wash port.

Simultaneously the peristaltic pump delivers the flush solvent.

10 The needle moves back. 11 The gripper arm moves out of the needle station and the wash port snaps

back in position.

only if automated needle wash is selected. If this feature is disabled, the gripper arm positions the sample vial directly below the needle (Step 4) and lowers the needle into the vial.

Agilent InfinityLab LC Series Vialsamplers User Manual 18

1Introduction

Vial

Wash port

Needle seat

Sampling loop

Solvent

Wash

pump

Metering device

Operating Principle

12 The gripper arm replaces the vial, and returns to the home position.

Simultaneously, the needle lowers into the seat.

13 The injection valve switches to the mainpass (main path) position.

Injection Sequence

Before the start of the injection sequence, and during an analysis, the injection valve is in the mainpass (main path) position (see position, the mobile phase flows through the autosamplers metering device, sample loop, and needle. This ensures that all parts in contact with sample are flushed during the run, thus minimizing carryover.

Figure 4 on page 19). In this

Figure 4 Mainpass (main path) Position - standard position during runs and when the sampler

idle

Agilent InfinityLab LC Series Vialsamplers User Manual 19

1Introduction

Vial

Wash port

Needle seat

Sampling loop

Solvent

Wash pump

Metering device

Operating Principle

When the sample sequence begins, the valve unit switches to the bypass position (see

Figure 5 on page 20). Solvent from the pump enters the valve unit at

port 1, and flows directly to the column through port 6.

Figure 5Valve in bypass - needle in vial, metering device aspirates sample volume

Agilent InfinityLab LC Series Vialsamplers User Manual 20

1Introduction

Vial

Wash

port

Needle seat

Sampling loop

Solvent

Wash pump

Metering device

Operating Principle

Then the vial is positioned below the needle. The needle moves down into the vial, the metering unit draws the required sample volume into the loop, and the needle is raised. In the next step, the needle is washed (see

Figure 6 on page 21).

Figure 6 Outer face of needle getting washed in wash port

Agilent InfinityLab LC Series Vialsamplers User Manual 21

1Introduction

Vial

Wash port

Needle seat

Sampling loop

Solvent

Wash pump

Metering device

Operating Principle

When the metering unit has drawn the required volume of sample into the sample loop, the vial is replaced in the sample tray. The wash port flips into the origin position, the needle is lowered into the needle seat, and the injection valve switches back to mainpass (main path) position, flushing the sample onto the column (see

Figure 7 on page 22).

Figure 7 Valve switches to mainpass (main path) - sample is transferred towards the LC column

Agilent InfinityLab LC Series Vialsamplers User Manual 22

1Introduction

Operating Principle

Needle Parkstation

The needle parkstation comprises two main assemblies: needle drive and wash port.

Figure 8Needle Station

Needle-Drive

The needle movement is driven by a stepper motor connected to the spindle assembly by a toothed belt. The circular motion of the motor is converted to linear motion by the drive nut on the spindle assembly. The upper and lower needle positions are detected by reflection sensors on the needle station board, while the needle-in-vial position is determined by counting the motor steps from the upper needle-sensor position.

Agilent InfinityLab LC Series Vialsamplers User Manual 23

1Introduction

NOTE

Operating Principle

Hydraulic Box

The hydraulic box comprises two main assemblies: metering device, and injection valve.

Figure 9 Hydraulic Unit

The replacement hydraulic box excludes the injection valve and metering head assemblies.

Agilent InfinityLab LC Series Vialsamplers User Manual 24

1Introduction

Operating Principle

Analytical Head

The analytical head is driven by the stepper motor that is connected to the drive shaft by a toothed belt. The drive nut on the spindle converts the circular movement of the spindle to linear motion. The drive nut pushes the sapphire piston against the tension of the spring into the analytical head. The base of the piston sits on the large bearing of the drive nut, which ensures the piston is always centered. A ceramic ring guides the movement of the piston in the analytical head. The home position of the piston is sensed by an optical sensor on the hydraulic unit board while the sample volume is determined by counting the number of steps from the home position. The backward movement of the piston (driven by the spring) draws sample from the vial.

Injection Valve

The two-position 6-port injection valve is driven by a stepper motor. Only five of the six ports are used (port 3 is not used). A lever/slider mechanism transfers the movement of the stepper motor to the injection valve. Two microswitches monitor switching of the valve (bypass and mainpass (main path) end positions). No valve adjustments are required after replacing internal components.

Agilent InfinityLab LC Series Vialsamplers User Manual 25

1Introduction

X motor

Theta motor

Gripper motor

Gripper arm

Z motor

Flex board

Theta axis

X axis

Z axis

Operating Principle

Transport Assembly

The transport unit comprises an X-axis slide (left-right motion), a Z-axis arm (up-down motion), and a gripper assembly (rotation and vial-gripping).

Figure 10 Transport Assembly

The transport assembly uses four stepper motors driven in closed-loop mode for accurate positioning of the gripper assembly. The rotational movement of the motors is converted to linear motion (X- and Z-axes) by toothed belts connected to the drive spindles. The rotation (theta axes) of the gripper assembly is transferred from the motor by a toothed belt and series of gears. The opening and closing of the gripper fingers are driven by a stepper motor linked by a toothed belt to the planetary gearing inside the gripper assembly.

The stepper motor positions are determined by the optical encoders mounted onto the stepper-motor housing. The encoders monitor the position of the motors continually, and correct for position errors automatically (e.g. if the gripper is accidentally moved out of position when loading vials into the vial tray). The initialization positions of the moving components are sensed by reflection sensors mounted on the flex board. These positions are used by the processor to calculate the actual motor position. An additional six reflection sensors for tray recognition are mounted on the flex board at the front of the assembly.

Agilent InfinityLab LC Series Vialsamplers User Manual 26

1Introduction

Leak and Waste Handling

The Agilent InfinityLab LC Series has been designed for safe leak and waste handling. It is important that all security concepts are understood and instructions are carefully followed.

The solvent cabinet is designed to store a maximum volume of 8 L solvent. The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 2 Cabinets (a printed copy of the guideline has been shipped with the solvent cabinet, electronic copies are available on the Internet).

All leak plane outlets are situated in a consistent position so that all Infinity and Infinity II modules can be stacked on top of each other. Waste tubes are guided through a channel on the right hand side of the instrument, keeping the front access clear from tubes.

The leak plane provides leak management by catching all internal liquid leaks, guiding them to the leak sensor for leak detection, and passing them on to the next module below, if the leak sensor fails. The leak sensor in the leak plane stops the running system as soon as the leak detection level is reached.

Solvent and condensate is guided through the waste channel into the waste container:

• from the detector's flow cell outlet

• from the Multisampler needle wash port

• from the Sample Cooler or Sample Thermostat (condensate)

• from the pump's Seal Wash Sensor (if applicable)

• from the pump's Purge Valve or Multipurpose Valve

L. For details, see the usage guideline for the Agilent Infinity II Solvent

Agilent InfinityLab LC Series Vialsamplers User Manual 27

1Introduction

Leak and Waste Handling

Figure 11 Infinity II Leak Waste Concept (Flex Bench installation)

Agilent InfinityLab LC Series Vialsamplers User Manual 28

1Introduction

Leak and Waste Handling

Figure 12 Infinity II Single Stack Leak Waste Concept (bench installation)

Agilent InfinityLab LC Series Vialsamplers User Manual 29

1Introduction

Leak and Waste Handling

Figure 13 Infinity II Two Stack Leak Waste Concept (bench installation)

The waste tube connected to the leak plane outlet on each of the bottom instruments guides the solvent to a suitable waste container.

Agilent InfinityLab LC Series Vialsamplers User Manual 30

1Introduction

CAUTION

NOTE

Leak and Waste Handling

Leak Sensor

Solvent incompatibility

The solvent DMF (dimethyl formamide) leads to corrosion of the leak sensor. The material of the leak sensor, PVDF (polyvinylidene fluoride), is incompatible with DMF.

 Do not use DMF as mobile phase.

 Check the leak sensor regularly for corrosion.

The leak sensor in the sampler is hidden under the ICC Column Heater or Column Shelf respectively.

Waste Guidance

Agilent InfinityLab LC Series Vialsamplers User Manual 31

The waste drainage must go straight into the waste containers. The waste flow must not be restricted at bends or joints.

1Introduction

Leak and Waste Handling

Waste Concept

1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with

4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don't immerse in the liquid.

Agilent InfinityLab LC Series Vialsamplers User Manual 32

2 Site Requirements and Specifications

Site Requirements 34 Physical Specifications 38 Performance Specifications 39

Performance Specifications (G7129A) 39 Performance Specifications (G7129B) 42 Performance Specifications (G7129C) 45

Specifications of the Sample Cooler 47 Specifications of the Sample Thermostat 49 Specifications of the Integrated Column Compartment 51

This chapter provides information on environmental requirements, physical and performance specifications.

Agilent InfinityLab LC Series Vialsamplers User Manual 33

2 Site Requirements and Specifications

WARNING

Site Requirements

A suitable environment is important to ensure optimum performance of the instrument.

Power Considerations

The module power supply has wide ranging capability. It accepts any line voltage in the range described in selector in the rear of the module. There are also no externally accessible fuses, because automatic electronic fuses are implemented in the power supply.

Hazard of electrical shock or damage of your instrumentation

can result, if the devices are connected to a line voltage higher than specified.



Connect your instrument to the specified line voltage only.

Table 1 on page 38. Consequently there is no voltage

Agilent InfinityLab LC Series Vialsamplers User Manual 34

Electrical shock hazard

The module is partially energized when switched off, as long as the power cord is plugged in.

The cover protects users from personal injuries, for example electrical shock.



Do not open the cover.



Do not operate the instrument and disconnect the power cable in case the cover has any signs of damage.



Contact Agilent for support and request an instrument repair service.

Inaccessible power plug.

In case of emergency it must be possible to disconnect the instrument from the power line at any time.



Make sure the power connector of the instrument can be easily reached and unplugged.



Provide sufficient space behind the power socket of the instrument to unplug the cable.

2 Site Requirements and Specifications

WARNING

Site Requirements

Power Cords

Country-specific power cords are available for the module. The female end of all power cords is identical. It plugs into the power-input socket at the rear. The male end of each power cord is different and designed to match the wall socket of a particular country or region.

Agilent makes sure that your instrument is shipped with the power cord that is suitable for your particular country or region.

Unintended use of power cords

Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment.



Never use a power cord other than the one that Agilent shipped with this instrument.



Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment.



Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.

Agilent InfinityLab LC Series Vialsamplers User Manual 35

Absence of ground connection

The absence of ground connection can lead to electric shock or short circuit.



Never operate your instrumentation from a power outlet that has no ground connection.

Electrical shock hazard

Solvents may damage electrical cables.



Prevent electrical cables from getting in contact with solvents.



Exchange electrical cables after contact with solvents.

2 Site Requirements and Specifications

WARNING

NOTE

Site Requirements

Room Size and Ventilation

Flammable refrigerant

Formation of flammable gas-air mixtures inside the Sample Thermostat and laboratory.



Keep open fire or sources of ignition away from the device.



Ensure a room size of 4 m3 (1 m3 for every 8 g of R600a refrigerant inside of the Sample Thermostat).



Ensure adequate ventilation: typical air exchange of 25 m3/h per m2 of laboratory floor area.



Keep all ventilation openings in the enclosure clear of obstructions. Do not block the openings on the circumference of the Sample Thermostat.

Bench Space

Agilent InfinityLab LC Series Vialsamplers User Manual 36

The module dimensions and weight (see Table 1 on page 38) allow you to place the module on almost any desk or laboratory bench. It needs an additional

2.5

cm (1.0 inches) of space on either side and approximately 8 cm (3.1 inches)

in the rear for air circulation and electric connections.

If the bench shall carry a complete HPLC system, make sure that the bench is designed to bear the weight of all modules.

The module should be operated in a horizontal position, especially if a Sample Cooler or Sample Thermostat is installed. Use a bubble level to check the leveling of the sampler.

Agilent recommends that you install the HPLC instrument in the InfinityLab Flex Bench rack. This option helps to save bench space as all modules can be placed into one single stack. It also allows to easily relocate the instrument to another laboratory.

2 Site Requirements and Specifications

WARNING

CAUTION

Site Requirements

Heavy weight

The module is heavy.



Carry the module at least with 2 people.



Avoid back strain or injury by following all precautions for lifting heavy objects.



Ensure that the load is as close to your body as possible.



Ensure that you can cope with the weight of your load.

Condensation

Condensation within the module

Condensation can damage the system electronics.

 Do not store, ship or use your module under conditions where temperature

fluctuations could cause condensation within the module.

Agilent InfinityLab LC Series Vialsamplers User Manual 37

 If your module was shipped in cold weather, leave it in its box and allow it to

warm slowly to room temperature to avoid condensation.

2 Site Requirements and Specifications

Physical Specifications

Tab le 1 Physical Specifications G7129A, G7129B, G7129C

Type Specification Comments

Weight 19 kg (41.9 lbs) w/o sample thermostat

Dimensions (height × width × depth)

Line voltage 100 – 240 V~, ± 10 % Wide-ranging capability

Line frequency 50 or 60 Hz, ± 5 %

Power consumption 350 VA, 350 W, 1195 BTU/h

Ambient operating temperature

320 x 396 x 468 mm (12.8 x 15.6 x 18.4 inches)

4 - 40 °C (39 - 104 °F); without sample thermostat up to 55 °C (131 °F)

Ambient non-operating temperature

Humidity

Operating altitude Up to 3000 m (9842 ft)

Safety standards: IEC, EN, CSA, UL

ISM Classification ISM Group 1 Class B According to CISPR 11

Permitted solvents Boiling point ≥56 °C (133 °F).

If a sample thermostat is included the upper value for humidity can be reduced. Please check your lab conditions to stay beyond dew point values for non–condensing operation.

-40 – 70 °C (-40 – 158 °F)

< 95 % r.h. at 40 °C (104 °F)

Installation category II, Pollution degree 2 For indoor use only.

Auto-ignition temperature ≥200 °C (394

°F).

Non-condensing

Agilent InfinityLab LC Series Vialsamplers User Manual 38

2 Site Requirements and Specifications

Performance Specifications

Performance Specifications (G7129A)

Tab le 2 Performance Specifications G7129A

Type Specification Comments Method/Conditions

Injection range 0.1 – 100 µL in 0.1 µL incre-

ments with 100 µL up to 60

MPa

0.1 – 900 µL in 0.1 µL increments with 900 µL up to 40

MPa

Injection precision <0.25 % RSD of peak areas from

µL to 100 µL

Pressure range 0 – 60 MPa (0 – 600 bar,

– 8702 psi)

0 0 – 40 MPa (0 – 400 bar, 0

– 5801 psi)

Sample viscosity range

Sample capacity 132 x 2 mL vial (two trays

0.2 – 5.0 cP

default) 100 x 2 mL vial (two classic trays optional) 36 x 6 mL vials (two trays optional)

Up to 1800 µL with multiple draw (hardware modification required) Requires 900 µL analytical head

Measured caffeine

for 900 µL Analytical Head

Agilent InfinityLab LC Series Vialsamplers User Manual 39

2 Site Requirements and Specifications

Performance Specifications

Table 2 Performance Specifications G7129A

Type Specification Comments Method/Conditions

Carryover <0.004 % (40 ppm) with needle

wash

Injection cycle time 18 s for draw speed 200 µL/min

Ejection speed: 200 µL/min Injection volume: 1 µL

Minimum sample volume

1 µL from 5 µL sample in 100 µL microvial, or 1 sample in 300

µL from 10 µL µL microvial.

Needle height offset has to be adapted to ensure that needle doesn’t touch vial bottom. Default needle height = 0 equates to 2 bottom.

mm above the vial

Using the following conditions:

• Column: Agilent Pursuit XRs 3 C18, 2.0 x 50

• Mobile Phase:

• A: 0.1 % TFA in water

• B: 0.1 % TFA in acetoni-

trile

• Isocratic : %B = 40 %

• Flow rate: 0.5 mL/min

• Temperature: Ambient

• Wavelength: 257/4 nm

• Injection volume: 1 µL

• Sample: 1200 ng/µL Chlor-

hexidine for UV (dissolved with mobile phase A), 1 injected and measured with Agilent UV detector

• Wash solution: H2O with

0.1

% TFA (3 s)

µL

Instrument Control LC & CE Drivers A.02.12 or

above Instrument Control Framework (ICF) A.02.03 or above Instant Pilot (G4208A) with firmware B.02.19 or above Lab Advisor B.02.07 or above

Communication Controller Area Network

(CAN),Local Area Network (LAN) ERI: ready, start, stop and shut-down signals

For details about supported software versions refer to the compatibility matrix of your ver sion of the LC & CE Drivers

Agilent InfinityLab LC Series Vialsamplers User Manual 40

2 Site Requirements and Specifications

Performance Specifications

Table 2 Performance Specifications G7129A

Type Specification Comments Method/Conditions

Maintenance and safety-related features

GLP features Early maintenance feedback

Housing All materials recyclable.

Extensive diagnostics, error detection and display with Agi lent Lab Advisor software Leak detection, safe leak handling, leak output signal for shutdown of pumping system, and low voltages in major main tenance areas

(EMF) for continuous tracking of instrument usage with user-settable limits and feed back messages. Electronic records of maintenance and errors.

Agilent InfinityLab LC Series Vialsamplers User Manual 41

2 Site Requirements and Specifications

Performance Specifications

Performance Specifications (G7129B)

Tab le 3 Performance Specifications G7129B

Type Specification Comments Method/Conditions

Injection range 0.1 – 20 µL in 0.1 µL increments

(default)

0.1 – 40 µL in 0.1 µL increments µL loop is installed)

(if 40

0.1 – 120 µL in 0.1 µL incre-

ments with 1290 Infinity large volume injection kit (hardware modification required)

0.1 – 100 µL in 0.1 µL (if 100 µL

loop and 100 installed)

Injection precision <0.25 % RSD of peak areas from

µL to 100 µL

Pressure range Up to 130 MPa (1300 bar,

18854

Sample viscosity range

Sample capacity 132 x 2 mL vial (two trays

0.2 – 5.0 cP

default) 100 x 2 mL vial (two classic trays optional) 36 x 6 mL vials (two trays optional)

µL head is

psi)

Up to 1500 µL (with 1400 µL multi-draw kit and 100 lytical head)

up to 130 MPa (1300 bar, 18854

psi)

up to 60 MPa (600 bar,

psi)

8702

Measured caffeine

µL ana-

Agilent InfinityLab LC Series Vialsamplers User Manual 42

2 Site Requirements and Specifications

Performance Specifications

Table 3 Performance Specifications G7129B

Type Specification Comments Method/Conditions

Carryover <0.004 % (40 ppm) with needle

wash

Injection cycle time 18 s for draw speed 200 µL/min

Ejection speed: 200 µL/min Injection volume: 1 µL

Minimum sample volume

1 µL from 5 µL sample in 100 µL microvial, or 1 sample in 300

µL from 10 µL µL microvial.

Needle height offset has to be adapted to ensure that needle doesn’t touch vial bottom. Default needle height = 0 equates to 2 bottom.

mm above the vial

Using the following conditions:

• Column: Agilent Pursuit XRs 3 C18, 2.0 x 50

• Mobile Phase:

• A: 0.1 % TFA in water

• B: 0.1 % TFA in acetoni-

trile

• Isocratic : %B = 40 %

• Flow rate: 0.5 mL/min

• Temperature: Ambient

• Wavelength: 257/4 nm

• Injection volume: 1 µL

• Sample: 1200 ng/µL Chlor-

hexidine for UV (dissolved with mobile phase A), 1 injected and measured with Agilent UV detector

• Wash solution: H2O with

0.1

% TFA (3 s)

µL

Instrument control LC & CE Drivers A.02.12 or

above Instrument Control Framework (ICF) A.02.03 or above Instant Pilot (G4208A) with firmware B.02.19 or above Lab Advisor B.02.07 or above

Communication Controller Area Network (CAN),

Local Area Network (LAN), ERI: ready, start, stop and shut-down signals

For details about supported software versions refer to the compatibility matrix of your ver sion of the LC & CE Drivers

Agilent InfinityLab LC Series Vialsamplers User Manual 43

2 Site Requirements and Specifications

Performance Specifications

Table 3 Performance Specifications G7129B

Type Specification Comments Method/Conditions

Maintenance and safety-related features

GLP features Early maintenance feedback

Housing All materials recyclable.

(EMF) for continuous tracking of instrument usage with user-settable limits and feed back messages. Electronic records of maintenance and errors.

Agilent InfinityLab LC Series Vialsamplers User Manual 44

2 Site Requirements and Specifications

Performance Specifications

Performance Specifications (G7129C)

Tab le 4 Performance Specifications G7129C

Type Specification Comments Method/Conditions

Injection range 0.1 – 100 µL in 0.1 µL incre-

ments with 100 µL up to 80

MPa

Injection precision <0.25 % RSD of peak areas from

µL to 100 µL

Pressure range 0 – 80 MPa (0 – 800 bar,

– 11603 psi)

Sample viscosity range

Sample capacity 132 x 2 mL vial (two trays

Carryover <0.004 % (40 ppm) with needle

0.2 – 5.0 cP

default) 100 x 2 mL vial (two classic trays optional) 36 x 6 mL vials (two trays optional)

wash

Up to 1800 µL with multiple draw (hardware modification required)

Measured caffeine

Using the following conditions:

• Column: Agilent Pursuit XRs 3 C18, 2.0 x 50

• Mobile Phase:

• A: 0.1 % TFA in water

• B: 0.1 % TFA in acetoni-

trile

• Isocratic : %B = 40 %

• Flow rate: 0.5 mL/min

• Temperature: Ambient

• Wavelength: 257/4 nm

• Injection volume: 1 µL

• Sample: 1200 ng/µL Chlor-

hexidine for UV (dissolved with mobile phase A), 1 injected and measured with Agilent UV detector

• Wash solution: H2O with

0.1

% TFA (3 s)

µL

Injection cycle time 18 s for draw speed 200 µL/min

Ejection speed: 200 µL/min Injection volume: 1 µL

Agilent InfinityLab LC Series Vialsamplers User Manual 45

2 Site Requirements and Specifications

Performance Specifications

Table 4 Performance Specifications G7129C

Type Specification Comments Method/Conditions

Minimum sample volume

Instrument Control LC & CE Drivers A.02.17 or

Communication Controller Area Network (CAN),

Maintenance and safety-related features

1 µL from 5 µL sample in 100 µL microvial, or 1 sample in 300

above Instrument Control Framework (ICF) A.02.05 or above Instant Pilot (G4208A) with firmware B.02.22 or above InfinityLab LC Companion (G7108A) Lab Advisor B.02.10 or above

Local Area Network (LAN), ERI: ready, start, stop and shut-down signals

µL from 10 µL µL microvial.

Needle height offset has to be adapted to ensure that needle doesn’t touch vial bottom. Default needle height = 0 equates to 2 bottom.

For details about supported software versions refer to the compatibility matrix of your ver sion of the LC & CE Drivers

mm above the vial

GLP features Early maintenance feedback

(EMF) for continuous tracking of instrument usage with user-settable limits and feed back messages. Electronic records of maintenance and errors.

Housing All materials recyclable.

Agilent InfinityLab LC Series Vialsamplers User Manual 46

2 Site Requirements and Specifications

Specifications of the Sample Cooler

The Agilent Infinity II Sample Cooler is a vapor-compression refrigeration system that uses a fluorinated greenhouse gas (HCF-134a) as the refrigerant. For information on carbon dioxide equivalency (CDE) and global warming potential (GWP), see the instrument label.

Tab le 5 Physical Specification of the Sample Cooler

Type Specification Comment

Weight < 6 kg (< 13.2 lbs)

Dimensions (height × width × depth) 205 x 340 x 370 mm

(8.1 x 13.4 x 14.6 inches)

Refrigerant gas HFC-134a (0.042 kg) Ozone depletion poten-

tial (ODP) = 0

Supply voltage 24 VDC

Current 10 A max.

Ambient operating temperature 4 – 40 ° C (39 – 104 ° F)

Ambient non-operating temperature -40 – 70 ° C (-40 – 158 ° F)

Humidity < 95 % r.h. at 40 °C (104 °F) Non-condensing

Operating altitude Up to 3000 m (9842 ft)

Safety standards: IEC, EN, CSA, UL

ISM Classification ISM Group 1 Class B According to CISPR 11

Installation category II, Pollution degree 2

For indoor use only

Agilent InfinityLab LC Series Vialsamplers User Manual 47

2 Site Requirements and Specifications

CAUTION

NOTE

Specifications of the Sample Cooler

General hazards and improper disposal

Improper disposal of the media and components used pollutes the environment.

 The disposal or scrapping of the Sample Cooler or the Sample Thermostat

must be carried out by a qualified disposal company.

 All media must be disposed of in accordance with national and local

regulations.

 Please contact your local Agilent Service Center in regard to safe

environmental disposal of the appliance or check www.agilent.com for more info.

Tab le 6 Performance Specifications of the Sample Cooler

Type Specifications

Operating principle High performance, low-energy consumption

micro-compressor based cooler with ozone-friendly HFC-134a coolant (42 g), user-upgradable.

Agilent InfinityLab LC Series Vialsamplers User Manual 48

Temperature range from 4 °C to 5 °C below ambient

Temperature settable from 4 – 40 °C in 1 ° increments

Temperature accuracy (<25 °C, <50 % r.H.) 2 °C to 6 °C at a setpoint of 4 °C

The Agilent Infinity II Sample Cooler is not available for trade sales anymore and has been replaced by the Agilent InfinityLab Sample Thermostat.

2 Site Requirements and Specifications

Specifications of the Sample Thermostat

The Agilent InfinityLab Sample Thermostat is the combination of an electric heater and a vapor-compression refrigeration system. It uses isobutane as a non-Freon refrigerant, which is harmless to the environment and does not affect the ozone layer and global warming but is combustible. Please adhere to the warnings listed in the manual.

Tab le 7 Physical Specifications of the Sample Thermostat

Type Specification Comments

Weight <6 kg (< 13.2 lbs)

Dimensions (height x width x depth)

Refrigerant gas R600a (0.030 kg) Ozone depletion potential (ODP) =0

Supply voltage 24VDC

205 x 340 x 370 mm (8.1 x 13.4 x 14.6 inches)

Global warming potential (GWP) =3

Current 10 A max.

Ambient operating temperature

Ambient non-operating temperature

Humidity < 95 % r.h. at 40 °C (104 °F) Non-condensing

Operating altitude Up to 3000 m (9842 ft)

Safety standards: IEC, EN, CSA, UL

ISM Classification ISM Group 1 Class B According to CISPR 11

4 – 40 °C (39 – 104 °F)

-40 – 70 °C (-40 – 158 °F)

Installation category II, Pollution degree 2

For indoor use only

Agilent InfinityLab LC Series Vialsamplers User Manual 49

2 Site Requirements and Specifications

CAUTION

NOTE

Specifications of the Sample Thermostat

General hazards and improper disposal

Improper disposal of the media and components used pollutes the environment.

 The disposal or scrapping of the Sample Cooler or the Sample Thermostat

must be carried out by a qualified disposal company.

 All media must be disposed of in accordance with national and local

regulations.

 Please contact your local Agilent Service Center in regard to safe

environmental disposal of the appliance or check www.agilent.com for more info.

Tab le 8 Performance Specifications for the Sample Thermostat

Type Specifications

Operating principle High performance, low-energy consumption micro-compressor based

cooler with natural R600a coolant (Butane 30

Temperature range from 4 – 40 °C

Temperature settable from 4 – 40 °C in 1 ° increments

g), user-upgradable

Agilent InfinityLab LC Series Vialsamplers User Manual 50

Temperature accuracy (<25 °C, <50 % r.H.)

2 °C to 6 °C at a setpoint of 4 °C

Minimum firmware revision for the Sample Thermostat is D.07.22.

Minimum LC driver revision for the Sample Thermostat is A.02.14.

2 Site Requirements and Specifications

Specifications of the Integrated Column Compartment

Physical Specifications Agilent InfinityLab LC Series Integrated Column Compartment (G7130A)

Tab le 9 Physical Specifications (G7130A)

Type Specification Comment

Weight 1.8 kg (4.0 lbs)

Dimensions (height x width x depth)

Supply Voltage 24 V DC

Power consumption 110 W

Ambient operating temperature 4 – 55 °C (39 – 131 °F)

Ambient non-operating temperature

Humidity

Operating altitude Up to 3000 m (9842 ft)

Safety standards IEC, EN, CSA, UL

ISM Classification ISM Group 1 Class B According to CISPR 11

If a sample cooler is included the upper value for humidity can be reduced. Please check your lab conditions to stay beyond dew point values for non–condensing operation.

86.5 x 396.0 x 106.5 mm (3.4 x 15.6 x 4.2 inches)

-40 – 70 °C (-40 – 158 °F)

< 95 % r.h. at 40 °C (104 °F)

Installation category II, Pollution degree 2

Maximum outside

Non-condensing

For indoor use only

Agilent InfinityLab LC Series Vialsamplers User Manual 51

2 Site Requirements and Specifications

Specifications of the Integrated Column Compartment

Performance Specifications Agilent InfinityLab LC Series Integrated Column Compartment (G7130A)

Tab le 10 Performance Specifications G7130A

Type Specification Comments

Temperature range 5 °C above ambient to 80 °C

Column capacity 2 columns up to 30 cm and

mm ID

4.6

Temperature stability ±0.10 °C at sensor

Temperature accuracy ±0.8 K (±0.5 K with calibration)

Warm up time 20 – 40 °C in 5 min at sensor

Agilent InfinityLab LC Series Vialsamplers User Manual 52

3Using the Module

Magnets 55 Turn on/off 56 Status Indicators 58 Vial Drawers and Trays 59

List of Drawers and Trays 59 Exchange Drawers 62 Insert a Vial Into the Sampler 65 Install the External Tray 67

Choice of Vials and Caps 70

Compatible Vials and Caps for the 2 mL Vial Drawers 70 Compatible Vials and Caps for the 6 mL Vial Drawer 73

Install the Optional Integrated Column Compartment 74

Unpacking the Unit 74 Install the Integrated Column Compartment 76 Install a Column in the ICC 82 Remove a Column from the ICC 86 Install the Column Shelf 88 Install the Column ID Upgrade Kit 92 Connect a Column Identification Tag to the Tag Reader 94

Using the Optional Integrated Column Compartment 96

Dashboard 96 Control Interface 97 Control 98 Method Parameters 99 Online Signals 101 Column Assignment 102 Column Tag Information Table 103 Using Column Identification Tags 104

Install the Optional Sample Cooler/Sample Thermostat 116

Unpacking the Unit 116 Install the Sample Cooler/Sample Thermostat 117

Using the Optional Sample Cooler/Sample Thermostat 123

Dashboard 123 Control Interface 124 Control 125 Temperature Mode 127 Online Signals 128

Agilent InfinityLab LC Series Vialsamplers User Manual 53

3Using the Module

Specifications of the Integrated Column Compartment

Reporting Sample Temperature 129 Operation Information 130

Transporting the Sampler 132

Prepare a Sampler Without Cooler/Thermostat for Transportation 132 Prepare a Sampler with Cooler/Thermostat for Transportation 133 Install the Transport Protection Foam 135

Agilent Local Control Modules 137

This chapter provides information on how to use the module.

Agilent InfinityLab LC Series Vialsamplers User Manual 54

3Using the Module

Magnets

1 Magnets in doors of pumps, autosamplers, detectors, and fraction collectors.

Agilent InfinityLab LC Series Vialsamplers User Manual 55

3Using the Module

Turn on/off

This procedure exemplarily shows an arbitrary LC stack configuration.

1 2

Power switch: On

Agilent InfinityLab LC Series Vialsamplers User Manual 56

3Using the Module

Turn on/off

3 Turn instrument On/Off with the control software. 4

Power switch: Off

Agilent InfinityLab LC Series Vialsamplers User Manual 57

3Using the Module

Status Indicators

This procedure exemplarily shows an arbitrary LC stack configuration. 1 The module status indicator indicates one of six possible module conditions:

Status indicators

1. Idle

2. Run mode

3. Not-ready. Waiting for a specific pre-run condition to be reached or completed.

4. Error mode - interrupts the analysis and requires attention (for example a leak or defective internal components).

5. Resident mode (blinking) - for example during update of main firmware.

6. Bootloader mode (fast blinking). Try to re-boot the module or try a cold-start. Then try a firmware update.

Agilent InfinityLab LC Series Vialsamplers User Manual 58

3Using the Module

NOTE

Vial Drawers and Trays

List of Drawers and Trays

Supported drawers for the module:

p/n Description

G7129-60010 Drawer for 66 x 2 mL Vials

G7129-60110 Drawer for 18 x 6 mL Vials

G7129-68210 Classic Vial Drawer Kit

G7129-60210 Classic Drawer for 50 x 2 mL Vials, Left

G7129-60220 Classic Drawer for 50 x 2 mL Vials, Right

G7129-60000 External Tray for 5 x 2 mL Vials

Install all drawers for best cooling performance.

Drawer Combinations

Drawers can be installed in any combination enabling both 2 mL- and 6 mL-vials to be used simultaneously. The only exception is the usage of the classical drawer option (100 x 2

mL). This option can't combine with the other drawers.

Numbering of Vial Positions

The standard 2*66 vial drawers have 132 vial positions from P1-A1-P2-F11. However, when using two drawers, the numbering convention is slightly different. The vial positions of the right-hand drawer begin at position P2-A1 as follows:

Left-hand Drawer for 66 x 2 mL Vials: P1-A1 to P1-F11

Left-hand Drawer for 18 x 6 mL Vials: P1-A1 to P1-C6

Right-hand Drawer for 66 x 2 mL Vials: P2-A1 to P2-F11

Right-hand Drawer for 18 x 6 mL Vials: P2-A1 to P2-C6

Drawer for 50 x 2 mL Vials Classic Left: Vial 1-50

Agilent InfinityLab LC Series Vialsamplers User Manual 59

3Using the Module

P1-A1

P1-F11

P2-A1

P2-F11

P1-A1

P1-C6

P2-A1

P2-C6

Vial Drawers and Trays

Drawer for 50 x 2 mL Vials Classic Right: Vial 51-100

External Tray 5-position: 201 – 205 Position

(The disposal tube is installed into the external tray by turning and pushing it into the backside of the hole position, No. 206)

Figure 14 Numbering of drawer position (left-hand Drawer for 66 x 2 mL Vials)

Figure 15 Numbering of drawer position (right-hand Drawer for 66 x 2 mL Vials)

Figure 16 Numbering of drawer position (left-hand Drawer for 18 x 6 mL Vials)

Figure 17 Numbering of drawer position (right-hand Drawer for 18 x 6 mL Vials)

Agilent InfinityLab LC Series Vialsamplers User Manual 60

3Using the Module

100

201

202

203

204

205

206 (Waste Disposal)

Vial Drawers and Trays

Figure 18 Numbering of drawer position (Drawer for 50 x 2 mL Vials Classic)

Agilent InfinityLab LC Series Vialsamplers User Manual 61

Figure 19 Numbering of tray position (External tray)

3Using the Module

NOTE

Vial Drawers and Trays

Exchange Drawers

Do not operate the sampler without drawers installed.

Install all drawers for best cooling performance.

Do not mix standard and classic drawers.

Install classic drawer 1-50 to the left, classic drawer 51-100 to the right side.

Agilent InfinityLab LC Series Vialsamplers User Manual 62

3Using the Module

Vial Drawers and Trays

1 Open the doors of the module. 2 Remove the drawer.

a Pull the drawer out.

b Lift the front of the drawer.

c Lift the drawer out.

d Remove the drawer.

Agilent InfinityLab LC Series Vialsamplers User Manual 63

3Using the Module

Vial Drawers and Trays

3 Install the drawer.

a Insert the back of the drawer.

b Align the drawer.

c Push in the drawer.

4 Close the doors.

Agilent InfinityLab LC Series Vialsamplers User Manual 64

3Using the Module

NOTE

Vial Drawers and Trays

Insert a Vial Into the Sampler

Attempting to insert a new vial into the sampler while the gripper arm is moving might lead to aborting the ongoing analysis.

1 Open the doors of the module.

For accessing the left drawer, it is sufficient to open the left door only.

3 Insert the vial into an appropriate position of the drawer. 4 Push the vial drawer back into place.

2 Pull out the vial drawer into which you want to insert the

vial.

Agilent InfinityLab LC Series Vialsamplers User Manual 65

3Using the Module

Vial Drawers and Trays

5 Close the doors.

Agilent InfinityLab LC Series Vialsamplers User Manual 66

3Using the Module

NOTE

Vial Drawers and Trays

Install the External Tray

Tools required Description

Flathead screwdriver

Parts required p/n Description

G7129-60000 External Tray for 5 x 2 mL Vials G1313-27302 Disposal tube

Preparations

• Turn off the sampler

• Open the doors

• Remove the samples

Keep foam and plastic cover in a safe place.

For best temperature performance, and if the external tray is not in use, it is best to cover the opening for the external tray with the original parts.

Agilent InfinityLab LC Series Vialsamplers User Manual 67

3Using the Module

Vial Drawers and Trays

1 Remove the foam and the plastic cover.

a Lift up the front part of the foam with a flathead

screw driver.

b Remove the foam.

2 Install the external tray and the disposal tube.

a Mount the external tray in the mounting holes on

the left side of the sampler and ensure that it is pushed in all the way.

b Install the disposal tube.

c Push out the plastic cover.

Agilent InfinityLab LC Series Vialsamplers User Manual 68

3Using the Module

Vial Drawers and Trays

3 Close the doors. 4 Configure the External Tray in the online view of the

Chromatographic Data System: right-click on the sam pler dashboard and select Modify> External Tray. In the dialog, select the External Tray installed check box.

Agilent InfinityLab LC Series Vialsamplers User Manual 69

3Using the Module

Choice of Vials and Caps

Compatible Vials and Caps for the 2 mL Vial Drawers

List of Compatible Vials and Caps

For reliable operation vials used with the autosampler must not have tapered shoulders or caps that are wider than the body of the vial. The vials in

Vials” on page 70, “Snap Top Vials” on page 71 and “Screw Top Vials” on page 71

and caps in “Crimp Caps” on page 72, “Snap Caps” on page 72 and “Screw

Caps” on page 72 (shown with their Part numbers) have been successfully tested

using a minimum of 15,000 injections with the autosampler.

Crimp Top Vials

p/n Description

“Crimp Top

5181-3375 Crimp Top Vial, 2 mL, clear glass, 100/Pack

5183-4491 Crimp Top Vial, 2 mL, clear glass, 1000/Pack

5182-0543 Crimp Top Vial, 2 mL, clear glass, write-on spot, 100/Pack

5183-4492 Crimp Top Vial, 2 mL, clear glass, write-on spot, 1000/Pack

5183-4494 Crimp Top Vial, 2 mL, clear glass, write-on spot, 100/Pack (silanized)

5181-3376 Crimp Top Vial, 2 mL, amber glass, write-on spot, 100/Pack

5183-4493 Crimp Top Vial, 2 mL, amber glass, write-on spot, 1000/Pack

5183-4495 Crimp Top Vial, 2 mL, amber glass, write-on spot, 100/Pack (silanized)

5182-0567 Crimp Top Vial, 1 mL, polypropylene, wide opening, 100/Pack

5183-4496 Crimp Top Vial, 1 mL, polypropylene, wide opening, 100/Pack

(silanized)

9301-0978 Crimp top vial, 250 µL, polypropylene, wide opening, 1000/Pack

Agilent InfinityLab LC Series Vialsamplers User Manual 70

3Using the Module

Choice of Vials and Caps

Snap Top Vials

p/n Description

5182-0544 Snap Top Vial, 2 mL, clear glass, 100/Pack

5183-4504 Snap Top Vial, 2 mL, clear glass, 1000/Pack

5183-4507 Snap Top Vial, 2 mL, clear glass, 100/Pack (silanized)

5182-0546 Snap Top Vial, 2 mL, clear glass, write-on spot, 100/Pack

5183-4505 Snap Top Vial, 2 mL, clear glass, write-on spot, 1000/Pack

5183-4508 Snap Top Vial, 2 mL, clear glass, write-on spot, 100/Pack (silanized)

5182-0545 Snap Top Vial, 2 mL, amber glass, write-on spot, 100/Pack

5183-4506 Snap Top Vial, 2 mL, amber glass, write-on spot, 1000/Pack

5183-4509 Snap Top Vial, 2 mL, amber glass, write-on spot, 100/Pack (silanized)

Screw Top Vials

p/n Description

5182-0714 Screw Cap Vials, 2 mL, clear glass, 100/Pack

5183-2067 Screw Top Vial, 2 mL, clear glass, 1000/Pack

5183-2070 Screw Top Vial, 2 mL, clear glass, 100/Pack (silanized)

5182-0715 Screw Top Vial, 2 mL, clear glass, write-on spot, 100/Pack

5183-2068 Screw Top Vial, 2 mL, clear glass, write-on spot, 1000/Pack

5183-2071 Screw Top Vial, 2 mL, clear glass, write-on spot, 100/Pack (silanized)

5182-0716 Screw Cap Vial, 2 mL, amber glass, write-on spot, 100/Pack

5183-2069 Screw Top Vial, 2 mL, amber glass, write-on spot, 1000/Pack

5183-2072 Screw Top Vial, 2 mL, amber glass, write-on spot, 100/Pack (silanized)

Agilent InfinityLab LC Series Vialsamplers User Manual 71

3Using the Module

Choice of Vials and Caps

Crimp Caps

p/n Description

5181-1210 Crimp Cap, silver aluminum, septum (clear PTFE/red rubber), 100/Pack

5183-4498 Crimp Cap, silver aluminum, septum (clear PTFE/red rubber), 1000/Pack

5181-1215 Crimp Cap, blue aluminum, septum (clear PTFE/red rubber), 100/Pack

5181-1216 Crimp Cap, green aluminum, septum (clear PTFE/red rubber), 100/Pack

5181-1217 Crimp Cap, red aluminum, septum (clear PTFE/red rubber), 100/Pack

Snap Caps

p/n Description

5182-0550 Snap Cap, clear polypropylene, septum (clear PTFE/red rubber), 100/Pack

5182-3458 Snap Cap, blue polypropylene, septum (clear PTFE/red rubber), 100/Pack

5182-3457 Snap Cap, green polypropylene, septum (clear PTFE/red rubber), 100/Pack

5182-3459 Snap Cap, red polypropylene, septum (clear PTFE/red rubber), 100/Pack

Screw Caps

p/n Description

5182-0717 Screw Cap, blue polypropylene, septum (clear PTFE/red rubber), 100/Pack

5182-0718 Screw Cap, green polypropylene, septum (clear PTFE/red rubber), 100/Pack

5182-0719 Screw Cap, red polypropylene, septum (clear PTFE/red rubber), 100/Pack

5182-0720 Screw Cap, blue polypropylene, septum (clear PTFE/silicone), 100/Pack

5182-0721 Screw Cap, green polypropylene, septum (clear PTFE/silicone), 100/Pack

5182-0722 Screw Cap, red polypropylene, septum (clear PTFE/silicone), 100/Pack

Agilent InfinityLab LC Series Vialsamplers User Manual 72

3Using the Module

NOTE

Choice of Vials and Caps

Compatible Vials and Caps for the 6 mL Vial Drawer

For reliable operation of the sampler, the combined height of the vial and cap should never exceed 40 mm.

Screw Top Vials and Caps

p/n Description

9301-1377 Screw Top Vial, 6 mL, clear glass, flat bottom, 100/Pack

5188-5369 Screw Top Vial, 5 mL, clear glass, high recovery, 100/Pack

9301-1379 Screw Cap, 16 mm, w/o septum, for 6 mL vials, 100/Pack

9301-1378 Septum, PTFE/silicone, for 16 mm caps, 100/Pack

5188-2758 Septum, preslit, PTFE/silicone, for 16 mm caps, 100/Pack

Crimp Top Vials and Caps

p/n Description

9301-1419 Crimp Top Vial, 6 mL, clear glass, flat bottom, 100/Pack

9301-1425 Crimp Cap, silver aluminum, septum (PTFE/silicone), 100/Pack

Agilent InfinityLab LC Series Vialsamplers User Manual 73

3Using the Module

CAUTION

Install the Optional Integrated Column Compartment

Unpacking the Unit

Damaged Packaging

If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the instrument may have been damaged during shipment.

"Defective on arrival" problems

If there are signs of damage, please do not attempt to install the module. Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged.

Agilent InfinityLab LC Series Vialsamplers User Manual 74

 Notify your Agilent sales and service office about the damage.

 An Agilent service representative will inspect the instrument at your site and

initiate appropriate actions.

Condensation

Condensation within the module

Condensation can damage the system electronics.

 Do not store, ship or use your module under conditions where temperature

fluctuations could cause condensation within the module.

 If your module was shipped in cold weather, leave it in its box and allow it to

warm slowly to room temperature to avoid condensation.

3Using the Module

Install the Optional Integrated Column Compartment

Delivery Checklist

Ensure all parts and materials have been delivered with your module. The delivery checklist is shown below. For parts identification please check the illustrated parts breakdown in report any missing or damaged parts to your local Agilent Technologies sales and service office.

Tab le 11 Delivery checklist for the Integrated Column Compartment (ICC)

Description Quantity

Integrated Column Compartment (G7130-60030 or G7130-60060) 1

Accessory Kit for ICC 1

“Integrated Column Compartment” on page 288. Please

Agilent InfinityLab LC Series Vialsamplers User Manual 75

3Using the Module

WARNING

Install the Optional Integrated Column Compartment

Install the Integrated Column Compartment

Tools required p/n Description

8710-0510 Open-end wrench 1/4 — 5/16 inch

Parts required # p/n Description

1 G7130-60030 Integrated Column Compartment, 3 µL Heater

OR 1 G7130-60060 Integrated Column Compartment, 6 µL Heater

1 Accessory Kit for ICC

(includes capillaries and column holder clips)

For more information on recommended capillary types refer to “Capillary

Connections” on page 278.

Preparations

• Switch off the pump.

• Switch off the sampler.

• Remove the column.

• Open the doors of the sampler.

Fire or explosion due to missing leak drainage

Solvents may run into the module if column shelf or Integrated Column Compartment are not installed.



Install either column shelf or Integrated Column Compartment before starting the module.

Agilent InfinityLab LC Series Vialsamplers User Manual 76

3Using the Module

Click

Install the Optional Integrated Column Compartment

1 Remove the column shelf.

a Press in the release buttons on the left and right

side.

b Pull out the column shelf.

2 Install the Integrated Column Compartment.

a Push in the Integrated Column Compartment.

b Ensure that the release buttons are clicked back in

their original position.

The column shelf is removed.

The Integrated Column Compartment is installed.

Agilent InfinityLab LC Series Vialsamplers User Manual 77

3Using the Module

Install the Optional Integrated Column Compartment

3 Push the latch of the Integrated Column Compartment

to the left.

5 Connect the injector valve (port 6) of the Vialsampler to

the inlet port of the ICC heat exchanger with a suitable capillary (see recommendations).

“Capillary Connections” on page 278 for

4 Open the flap door.

6 Connect a suitable capillary to the respective outlet port

of the ICC heat exchanger (see

Connections” on page 278 for recommendations).

“Capillary

Agilent InfinityLab LC Series Vialsamplers User Manual 78

3Using the Module

NOTE

max.

2.5 Nm

max.

2.5 Nm

Install the Optional Integrated Column Compartment

7 Tighten the capillary fitting to make the connection

pressure tight.

Do not overtighten the fitting (maximum torque 2.5 Nm).

The use of capillaries with SL or SX fittings are recommended for a better handling.

8 Mount the column holders on the heat exchanger.

Agilent InfinityLab LC Series Vialsamplers User Manual 79

3Using the Module

Install the Optional Integrated Column Compartment

9 Connect the column to the free end of the capillary

coming from the ICC heat exchanger. For best perfor mance, use capillaries with the InfinityLab Quick Connect Fitting.

a Turn the column onto the Quick Connect Fitting

until the first resistance is observed.

b Close the lever to establish a pressure tight connec-

tion.

10 Connect a suitable capillary to the outlet port of the col-

umn. For best performance, use capillaries with the InfinityLab Quick Turn Fitting.

a Connect the capillary with the Quick Connect Fitting

and tighten it until finger-tightness.

The fitting should be pressure tight up to 600 bar.

b If needed, tighten the fitting further with the help of

two suitable wrenches.

Agilent InfinityLab LC Series Vialsamplers User Manual 80

3Using the Module

NOTE

Click

Install the Optional Integrated Column Compartment

11 Mount the column in the column holder clip(s). 12 Route the capillary coming from the column through the

opening between the flap door and the ICC body towards the detector module.

Alternatively, the capillary can also be routed through the incisions at the right side of the ICC dedicated for this pur pose.

13 Close the flap door until the latch is locked. 14 Close the doors.

15 Turn on the sampler and the pump.

Agilent InfinityLab LC Series Vialsamplers User Manual 81

3Using the Module

NOTE

Install the Optional Integrated Column Compartment

Install a Column in the ICC

Tools required p/n Description

8710-0510 Open-end wrench 1/4 — 5/16 inch

Parts required p/n Description

Column (up to 30 cm length)

G7116-68003 Column Holder Lamella, 2/pk

For more information on recommended capillary types, refer to “Capillary

Connections” on page 278.

Preparations

• Switch off the pump

• Switch off the sampler

• Open the doors of the sampler

A maximum of two columns can be installed.

1 Push the latch of the Integrated Column Compartment

to the left.

2 Open the flap door.

Agilent InfinityLab LC Series Vialsamplers User Manual 82

3Using the Module

NOTE

max.

2.5 Nm

max.

2.5 Nm

Install the Optional Integrated Column Compartment

3 Connect the injector valve (port 6) of the Vialsampler to

the inlet port of the ICC heat exchanger with a suitable capillary (see recommendations).

5 Tighten the capillary fitting to make the connection

pressure tight.

Do not overtighten the fitting (maximum torque 2.5 Nm).

The use of capillaries with SL or SX fittings are recommended for a better handling.

“Capillary Connections” on page 278 for

4 Connect a suitable capillary to the respective outlet port

of the ICC heat exchanger (see

Connections” on page 278 for recommendations).

6 Mount the column holders on the heat exchanger.

“Capillary

Agilent InfinityLab LC Series Vialsamplers User Manual 83

3Using the Module

Install the Optional Integrated Column Compartment

7 Connect the column to the free end of the capillary

coming from the ICC heat exchanger. For best perfor mance, use capillaries with the InfinityLab Quick Connect Fitting.

a Turn the column onto the Quick Connect Fitting

until the first resistance is observed.

b Close the lever to establish a pressure tight connec-

tion.

8 Connect a suitable capillary to the outlet port of the col-

umn. For best performance, use capillaries with the InfinityLab Quick Turn Fitting.

a Connect the capillary with the Quick Connect Fitting

and tighten it until finger-tightness.

The fitting should be pressure tight up to 600 bar.

b If needed, tighten the fitting further with the help of

two suitable wrenches.

Agilent InfinityLab LC Series Vialsamplers User Manual 84

3Using the Module

NOTE

Click

Install the Optional Integrated Column Compartment

9 Mount the column in the column holder clip(s). 10 Route the capillary coming from the column through the

opening between the flap door and the ICC body towards the detector module.

Alternatively, the capillary can also be routed through the incisions at the right side of the ICC dedicated for this pur pose.

11 Close the flap door until the latch is locked. 12 Close the doors.

Next Steps:

13 If applicable, connect the column identification tag to the tag reader (see “Connect a Column Identification Tag to the

Tag Reader” on page 94).

14 Configure the connected column in the CDS (see “Column Assignment” on page 102).

Agilent InfinityLab LC Series Vialsamplers User Manual 85

3Using the Module

Install the Optional Integrated Column Compartment

Remove a Column from the ICC

Tools required p/n Description

8710-0510 Open-end wrench 1/4 — 5/16 inch

Preparations

1 Push the latch of the Integrated Column Compartment

to the left.

3 Remove the column from the column holder clips. 4 Loosen the Quick Turn Fitting either with your fingers or

• Switch off the pump

• Switch off the sampler

• Open the doors of the sampler

2 Open the flap door.

with the help of two wrenches.

Agilent InfinityLab LC Series Vialsamplers User Manual 86

3Using the Module

Click

Install the Optional Integrated Column Compartment

5 Remove the capillary from the outlet port of the column. 6 Lift up the lever to release the tension on the Quick Con-

nect Fitting.

7 Unscrew and remove the Quick Connect Fitting. 8 Remove the column from the ICC.

9 Close the flap door until the latch is locked.

Agilent InfinityLab LC Series Vialsamplers User Manual 87

3Using the Module

WARNING

Install the Optional Integrated Column Compartment

Install the Column Shelf

Tools required p/n Description

8710-0510 Open-end wrench 1/4 — 5/16 inch

Parts required p/n Description

G7129-60023 Column Shelf

Preparations

• Switch off the pump.

• Switch off the sampler.

• Open the doors of the sampler.

• Open the ICC flap door.

Fire or explosion due to missing leak drainage

Solvents may run into the module if column shelf or Integrated Column Compartment are not installed.



Install either column shelf or Integrated Column Compartment before starting the module.

1 Remove the capillary connecting the injection valve and

the ICC heat exchanger.

2 Remove the column from the column holder clips.

Agilent InfinityLab LC Series Vialsamplers User Manual 88

3Using the Module

Click

Install the Optional Integrated Column Compartment

3 Loosen the Quick Turn Fitting either with your fingers or

with the help of two wrenches.

5 Lift up the lever to release the tension on the Quick Con-

nect Fitting.

4 Remove the capillary from the outlet port of the column.

6 Unscrew and remove the Quick Connect Fitting.

7 Remove the column from the ICC. 8 Close the flap door until the latch is locked.

Agilent InfinityLab LC Series Vialsamplers User Manual 89

3Using the Module

Click

Install the Optional Integrated Column Compartment

9 Remove the Integrated Column Compartment.

a Press the release buttons on the left and right side.

b Pull out the Integrated Column Compartment.

10 Install the column shelf

a Push in the column shelf.

b Ensure that the release buttons are clicked back in

their original position.

The Integrated Column Comparment is removed.

The column shelf is installed.

Agilent InfinityLab LC Series Vialsamplers User Manual 90

3Using the Module

Install the Optional Integrated Column Compartment

11 Close the doors. 12 Turn on the sampler and the pump.

Agilent InfinityLab LC Series Vialsamplers User Manual 91

3Using the Module

NOTE

Install the Optional Integrated Column Compartment

Install the Column ID Upgrade Kit

Parts required # p/n Description

1 G4751A Column ID Upgrade Kit for the Integrated Column Compartment

(includes the tag reader, 2 column identification tags and a technical note)

OR 1 5067-6153 Column ID Tag Reader

1 5067-5917 InfinityLab Column Identification Tag

Preparations

• Power off the instrument.

• Install the Integrated Column Compartment (see “Install the Integrated Column

Compartment” on page 76).

To use the G4751A Column ID Upgrade Kit, the Agilent Integrated Column Compartment must be installed in the Vialsampler.

1 Place the two mounting clips onto the left side of the

Vialsampler. Make sure of the correct orientation.

3 Connect the cable of the tag reader to the respective

socket on the back of the Vialsampler.

2 Mount the tag reader to the mounting clips.

4 Fix the cable to the back of the sampler with the cable

clips provided with the tag reader.

Agilent InfinityLab LC Series Vialsamplers User Manual 92

3Using the Module

Install the Optional Integrated Column Compartment

Next Steps:

5 Power on the instrument.

6 Place a column with an identification tag in the ICC and plug the tag into the tag reader (see “Connect a Column Identi-

fication Tag to the Tag Reader” on page 94).

Agilent InfinityLab LC Series Vialsamplers User Manual 93

3Using the Module

NOTE

Install the Optional Integrated Column Compartment

Connect a Column Identification Tag to the Tag Reader

Tools required p/n Description

8710-0510 Open-end wrench 1/4 — 5/16 inch

Parts required p/n Description

5067-5917 InfinityLab Column Identification Tag

Column (up to 30 cm length)

Preparations

1 If the column is without a preinstalled identification tag,

fit a new tag onto it by slipping the lanyard loop over one end of the column and pulling the cord tight through the plastic holder.

Once the lanyard is fixed, the tag can no longer be removed from the column.

• Install the Column ID Tag Reader (see “Install the Column ID Upgrade Kit” on page 92).

• Remove the column if installed (see “Remove a Column from the ICC” on page 86).

2 Install the column in the ICC (see “Install a Column in

the ICC” on page 82).

Agilent InfinityLab LC Series Vialsamplers User Manual 94

3Using the Module

Click

Install the Optional Integrated Column Compartment

3 Route the cord to the left and plug the column identifi-

cation tag into the corresponding socket of the tag reader.

5 Close the doors. 6 Configure the connected column with identification tag

4 Close the flap door until the latch is locked.

in the CDS (see

“Using Column Identification Tags” on page 104).

“Column Assignment” on page 102 and

Agilent InfinityLab LC Series Vialsamplers User Manual 95

3Using the Module

Using the Optional Integrated Column Compartment

The following section describes how to operate the Agilent Integrated Column Compartment.

Dashboard

When the Integrated Column Compartment (ICC) is installed in a Vialsampler and configured in the chromatography data system (CDS), a new tile appears on the dashboard, comprising a standalone graphical user interface (GUI) for the ICC.

1 ICC: Turn On/Off

2 ICC: Heater Status (On/Off)

3 ICC: Actual Temperature

4 ICC: Set Temperature

5 ICC: Column Information

6 ICC: Status Indicator

7 ICC: Early Maintenance Feedback (EMF) Status

8 ICC: Column Identification Tag Recognised

Agilent InfinityLab LC Series Vialsamplers User Manual 96

3Using the Module

Using the Optional Integrated Column Compartment

Control Interface

Right-clicking on the sampler GUI opens the control interface, where control and method parameters can be edited, column assignment modified, and special commands executed.

Agilent InfinityLab LC Series Vialsamplers User Manual 97

3Using the Module

Using the Optional Integrated Column Compartment

Control

The Control dialog box enables the user to change some control-specific settings for the ICC:

•Thermostat:

• On: The ICC turns on and the system starts to regulate the temperature of

the column oven towards the setpoint.

• Off: The ICC turns off.

•At Power On:

• Turn on thermostat: The ICC turns on automatically upon powering on the

sampler.

•Automatic Turn On:

• Turn on at: The ICC turns on automatically at the specified time and date.

Agilent InfinityLab LC Series Vialsamplers User Manual 98

3Using the Module

NOTE

Using the Optional Integrated Column Compartment

Method Parameters

The Method dialog box enables the user to change the method parameters relevant for the ICC:

• Temperature:

• Not Controlled: The temperature of the column oven stays uncontrolled.

• At specified temperature (°C): The temperature of the column oven is

regulated to stay at the setpoint temperature specified.

Valid inputs are 10 to 80 °C in increments of 0.1 °C.

• Unchanged: The temperature setting stays unchanged, meaning that the

setting currently stored in the system is used by the method.

•Valve Position/Column: This section provides information about the location of the column that is being used for the given method.

• Enforce column for run: Marking this checkbox restricts the execution of

the method to a specific column type, identified by its product number.

•Stoptime:

• As Pump/Injector: The ICC uses the same stoptime setting as specified for

the pump or the sampler.

• After specified time (min): The stop time defines a time limit for the

analysis, after which all method parameters are reset to initial values.

•Posttime:

• Off: No post-run period is applied.

• For specified time (min): After the analysis, the system stays in the

post-run state for the specified time. Using a post-time period can be beneficial when the column needs to be re-equilibrated before starting the next analysis (for example, gradient elutions).

For Stoptime and Posttime, valid inputs are 0.01 to 99999 minutes in increments of 0.1 min.

Agilent InfinityLab LC Series Vialsamplers User Manual 99

3Using the Module

Using the Optional Integrated Column Compartment

• Enable Analysis:

• With any temperature: The analysis starts regardless of the actual column

oven temperature.

• When temperature is within: The analysis starts only when the actual

column oven temperature is within the specified range of the setpoint temperature.

•Timetable: This feature enables the user to use programmed changes to control the oven temperature during the curse of the analysis.

Agilent InfinityLab LC Series Vialsamplers User Manual 100

Agilent InfinityLab LC User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

In This Book:

1Introduction

Product Description (G7129A)

Features (G7129A)

Product Description (G7129B)

Features (G7129B)

Product Description (G7129C)

Features (G7129C)

Overview of the Module

Operating Principle

Sampling Sequence

Needle Parkstation

Hydraulic Box

Transport Assembly

Leak and Waste Handling

Leak Sensor

Waste Guidance

Waste Concept

2 Site Requirements and Specifications

Site Requirements

Site Requirements

Power Considerations

Power Cords

Room Size and Ventilation

Bench Space

Condensation

Physical Specifications

Performance Specifications

Performance Specifications (G7129A)

Performance Specifications (G7129B)

Performance Specifications (G7129C)

Specifications of the Sample Cooler

Specifications of the Sample Thermostat

Specifications of the Integrated Column Compartment

Physical Specifications Agilent InfinityLab LC Series Integrated Column Compartment (G7130A)

Performance Specifications Agilent InfinityLab LC Series Integrated Column Compartment (G7130A)

3Using the Module

Magnets

Turn on/off

Status Indicators

Vial Drawers and Trays

List of Drawers and Trays

Exchange Drawers

Insert a Vial Into the Sampler

Install the External Tray

Choice of Vials and Caps

Compatible Vials and Caps for the 2 mL Vial Drawers

Compatible Vials and Caps for the 6 mL Vial Drawer

Install the Optional Integrated Column Compartment

Unpacking the Unit

Install the Integrated Column Compartment

Install a Column in the ICC

Remove a Column from the ICC

Install the Column Shelf

Install the Column ID Upgrade Kit

Connect a Column Identification Tag to the Tag Reader

Using the Optional Integrated Column Compartment

Dashboard

Control Interface

Control

Method Parameters