Agilent InfinityLab LC User Manual

Vialsamplers

Agilent InfinityLab LC Series

User Manual

Agilent InfinityLab LC Series Vialsamplers User Manual

Notices

CAUTION

WARNING

Document Information

Document No: SD-29000242 Rev. E Part No: G7129-90010 Rev. E EDITION 04/2020

No part of this manual may be reproduced in any form or by any means (including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent from Agilent Technologies, Inc. as governed by United States and international copyright laws.

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The material contained in this document is provided “as is,” and is subject to being changed, without notice, in future editions. Further, to the maximum extent permitted by applicable law, Agilent disclaims all warranties, either express or implied, with regard to this manual and any information contained herein, including but not limited to the implied warranties of merchantability and fitness for a particular purpose. Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing, use, or performance of this document or of any information contained herein. Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms, the warranty terms in the separate agreement shall control.

Technology Licenses

The hardware and/or software described in this document are furnished under a license and may be used or copied only in accordance with the terms of such license.

Safety Notices

CAUTION

hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a the indicated conditions are fully understood and met.

A WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in personal injury or death. Do not proceed beyond a WARNING notice until the indicated conditions are fully understood and met.

notice denotes a

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notice until

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Agilent InfinityLab LC Series Vialsamplers User Manual

In This Book:

This manual covers the following modules:

• Agilent 1260 Infinity II Vialsampler (G7129A)

• Agilent 1290 Infinity II Vialsampler (G7129B)

• Agilent 1260 Infinity II Vialsampler (G7129C)

1Introduction

This chapter gives an introduction to the module and instrument overview.

2 Site Requirements and Specifications

This chapter provides information on environmental requirements, physical and performance specifications.

3 Using the Module

This chapter provides information on how to use the module.

4 Preparing the Module

This chapter explains the operational parameters of the module.

5 Optimizing Performance

This chapter provides information on how to optimize the module.

6Troubleshooting and Diagnostics

This chapter gives an overview of the maintenance, troubleshooting, and diagnostic features available for the Agilent InfinityLab Series Vialsampler.

7Error Information

This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions.

Agilent InfinityLab LC Series Vialsamplers User Manual 3

8 Maintenance

This chapter describes the maintenance of the module.

9 Parts and Materials for Maintenance

This chapter provides information on parts for maintenance.

10 Identifying Cables

This chapter provides information on cables used with the Agilent 1200 Infinity Series modules.

11 Hardware Information

This chapter describes the detector in more detail on hardware and electronics.

12 LAN Configuration

This chapter provides information on connecting the module to the Agilent ChemStation PC.

13 Appendix

This chapter provides additional information on safety, legal and web.

Agilent InfinityLab LC Series Vialsamplers User Manual 4

Contents

1Introduction 9

Product Description (G7129A) 10 Features (G7129A) 11 Product Description (G7129B) 12 Features (G7129B) 13 Product Description (G7129C) 14 Features (G7129C) 15 Overview of the Module 16 Operating Principle 18 Leak and Waste Handling 27

2 Site Requirements and Specifications 33

Site Requirements 34 Physical Specifications 38 Performance Specifications 39 Specifications of the Sample Cooler 47 Specifications of the Sample Thermostat 49 Specifications of the Integrated Column Compartment 51

3 Using the Module 53

Magnets 55 Turn on/off 56 Status Indicators 58 Vial Drawers and Trays 59 Choice of Vials and Caps 70 Install the Optional Integrated Column Compartment 74 Using the Optional Integrated Column Compartment 96 Install the Optional Sample Cooler/Sample Thermostat 116 Using the Optional Sample Cooler/Sample Thermostat 123 Transporting the Sampler 132 Agilent Local Control Modules 137

Agilent InfinityLab LC Series Vialsamplers User Manual 5

4 Preparing the Module 139

Leak and Waste Handling 140 Preparing the Module 141 Solvent Information 142 Capillary Color Coding Guide 149 Installing Capillaries 150 Flow Connections to the Vialsampler 152 Setting up the Vialsampler 154

5 Optimizing Performance 166

Optimization for Lowest Carryover 167 Fast Injection Cycle and Low Delay Volume 171 Precise Injection Volume 173 Choice of Rotor Seal 175

6 Troubleshooting and Diagnostics 176

Status Indicators 177 Overview of Tests and Tools 178 Maintenance and Troubleshooting Tools 179 Diagnostic Tests 187 Agilent Lab Advisor Software 189

7Error Information 190

What are Error Messages 192 General Error Messages 193 Vialsampler Error Messages 201 Sample Cooler/Sample Thermostat Error Messages 213 Integrated Column Compartment (ICC) Heater Error Messages 219

Agilent InfinityLab LC Series Vialsamplers User Manual 6

8 Maintenance 222

Introduction to Maintenance 223 Warnings and Cautions 224 Overview of Maintenance 226 Cleaning the Module 227 Remove and Install Doors 228 Exchange the Needle Assembly 229 Exchange the Needle Seat Assembly 235 Exchange the Sample Loop Assembly 241 Exchange the Rotor Seal 245 Exchange the Metering Seal and Piston 250 Replace the Analytical Heads 255 Exchange the Gripper Arm 258 Replace the Peristaltic Pump Cartridge 261 Exchange the Wash Port Assembly 263 Replace the Module Firmware 269 Replace the Sample Cooler/Sample Thermostat 270

9 Parts and Materials for Maintenance 275

Standard Parts For Maintenance 276 Accessory Kits 279 Vial Drawers and Trays 283 Multidraw Kits 286 Sample Thermostat Upgrade 287 Integrated Column Compartment 288 Column ID Upgrade Kit 289 Parts for 900 µL Injection Upgrade 290 Cabinet and Door Kits 291 Analytical Head Assembly (100 µL) 295 Analytical-Head Assembly (900 µL) 296 Analytical Head Assembly (40 μL) 297 2 Position/6 Port Injection Valve, 600 bar 298 2 Position/6 Port Injection Valve, 800 bar 299 2 Position/6 Port Injection Valve, 1300 bar 300

Agilent InfinityLab LC Series Vialsamplers User Manual 7

10 Identifying Cables 301

Cable Overview 302 Analog Cables 304 Remote Cables 306 CAN/LAN Cables 310 RS-232 Cables 311 USB 312

11 Hardware Information 313

Firmware Description 314 Electrical Connections 317 Interfaces 319 Setting the 6-bit Configuration Switch 326 Instrument Layout 330 Early Maintenance Feedback (EMF) 331

12 LAN Configuration 332

What You Have to Do First 333 TCP/IP parameter configuration 334 Configuration Switches 335 Initialization Mode Selection 336 Dynamic Host Configuration Protocol (DHCP) 338 Manual Configuration 341 PC and Agilent ChemStation Setup 345

13 Appendix 354

General Safety Information 355 Waste Electrical and Electronic Equipment (WEEE) Directive 361 Refrigerant 362 Radio Interference 365 Sound Emission 366 Solvent Information 367 Agilent Technologies on Internet 368

Agilent InfinityLab LC Series Vialsamplers User Manual 8

1Introduction

Sampling Sequence 18 Needle Parkstation 23 Hydraulic Box 24 Transport Assembly 26

Leak and Waste Handling 27

Leak Sensor 31 Waste Guidance 31 Waste Concept 32

This chapter gives an introduction to the module and instrument overview.

Agilent InfinityLab LC Series Vialsamplers User Manual 9

1Introduction

Status indicator

Needle assembly

Drawer

Column shelf

Leak drain

Power switch

Product Description (G7129A)

The Agilent 1260 Infinity II Vialsampler is an autosampler designed for the reliability and ease-of-use needed for routine pharmaceutical tasks and quality control, as well as for environmental and food analyses. It can house optionally the integrated column compartment for two LC columns with temperature control up to 80 4

°C to 40 °C, all within one module.

°C as well as a sample thermostat for stable temperatures from

Agilent InfinityLab LC Series Vialsamplers User Manual 10

Figure 1 Overview of the Vialsampler

1Introduction

Features (G7129A)

• High capacity – up to 132 vials (2 mL) or up to 36 vials (6 mL).

• Reliable injections – 0.1 to 100 µL injections for up to 600 bar (G7129A) and

up to 800

• Easy volume extensions – for injection volumes up to 1800 µL for applications ranging from microbore to semipreparative chromatography.

•Lowest carryover – with an in needle flush port included, for rinsing the outside of the needle.

• Efficient temperature control – with an integrated column compartment as option or upgrade available. Which holds two columns up to 30 and provides heating capacity from 5 reproducible chromatography data at optimized resolution.

• Integrated sample thermostat – available as option or upgrade, providing cooling and heating in the range from 4

• Low internal volume – for minimum contribution to a system's total internal volume, which can be even further reduced using "bypass" mode.

•Increased productivity – with overlapped injections.

• Customizable injection program – available for customizing advanced

injections as well as for sample preparation steps upfront injection.

bar (G7129C).

cm length,

°C above ambient up to 80 °C for

°C - 40 °C.

Agilent InfinityLab LC Series Vialsamplers User Manual 11

1Introduction

Status indicator

Needle assembly

Drawer

Column shelf

Leak drain

Power switch

Product Description (G7129B)

The Agilent 1290 Infinity II Vialsampler (G7129B) is an autosampler designed for UHPLC applications up to 1300 ease-of-use needed for routine pharmaceutical tasks and quality control, as well as for environmental and food analyses. It can house optionally the integrated column compartment for two LC columns with temperature control up to 80 as well as a sample thermostat for stable temperatures from 4 within one module.

bar. It provides the reliability, safety, and

°C

°C to 40 °C, all

Agilent InfinityLab LC Series Vialsamplers User Manual 12

Figure 2 Overview of the Vialsampler

1Introduction

Features (G7129B)

• Accurate and precise injections - within a wide and flexible range of volumes

• High capacity - up to 132 vials (2 mL) or up to 36 vials (6 mL).

• Easy volume extensions - for injection volumes up to 1500 µL for applications

ranging from microbore to semipreparative chromatography.

•Lowest carryover - with an in needle flush port included, for rinsing the outside of the needle.

• Efficient temperature control - with an integrated column compartment as option or upgrade available. Which holds two columns up to 30 and provides heating capacity from 5 reproducible chromatography data at optimized resolution.

• Integrated sample thermostat - available as option or upgrade, providing cooling and heating in the range from 4

• Low internal volume - for minimum contribution to a system's total internal volume, which can be even further reduced using bypass mode.

•Increased productivity - with overlapped injections.

• Customizable Injection program - available for customizing advanced

injections as well as for sample preparation steps upfront injection.

°C above ambient up to 80 °C for

– 40 °C.

cm length,

Agilent InfinityLab LC Series Vialsamplers User Manual 13

1Introduction

Status indicator

Needle assembly

Drawer

Column shelf

Leak drain

Power switch

Product Description (G7129C)

°C to 40 °C, all within one module.

°C as well as a sample thermostat for stable temperatures from

Agilent InfinityLab LC Series Vialsamplers User Manual 14

Figure 3 Overview of the Vialsampler

1Introduction

Features (G7129C)

• High capacity – up to 132 vials (2 mL) or up to 36 vials (6 mL).

• Reliable injections – 0.1 to 100 µL injections for up to 600 bar (G7129A) and

up to 800

• Easy volume extensions – for injection volumes up to 1800 µL for applications ranging from microbore to semipreparative chromatography.

•Lowest carryover – with an in needle flush port included, for rinsing the outside of the needle.

• Integrated sample thermostat – available as option or upgrade, providing cooling and heating in the range from 4

• Low internal volume – for minimum contribution to a system's total internal volume, which can be even further reduced using "bypass" mode.

•Increased productivity – with overlapped injections.

• Customizable injection program – available for customizing advanced

injections as well as for sample preparation steps upfront injection.

bar (G7129C).

cm length,

°C above ambient up to 80 °C for

°C - 40 °C.

Agilent InfinityLab LC Series Vialsamplers User Manual 15

1Introduction

Overview of the Module

The Agilent InfinityLab LC Series Vialsampler is designed for use with other modules of the Agilent InfinityLab LC Series, 1200 Series, and 1100 Series LC, as well as with other LC systems with adequate remote-control capabilities. The Vialsampler can be controlled from any computer with a suitable chromatography data system (for example, Agilent OpenLab CDS, MassHunter, etc.) or via an Agilent local control module (InfinityLab Companion or 1200 Infinity Series Instant Pilot).

The 1290 Infinity II Vialsampler (G7129B) can be operated at up to 1300 bar. The two 1260 Infinity II Vialsampler variants (G7129A and G7129C) are limited to a maximum system pressure of 600 and 800

The Vialsampler can accommodate two vial drawers, providing space for up to 132 samples. The two cartesian vial drawers allow for the use of either 66 × 2 or 18 × 6 system is also available, each capable of hosting 50 × 2 drawers are designed to ease method transfer from older Agilent samplers. The Vialsampler can also be equipped with an external tray, which can be beneficial for automation. The Agilent WalkUp solution fully supports the external tray.

mL vials. A pair of classic vial drawers with a numerical vial assignment

bar, respectively.

mL vials. The classic vial

The Vialsampler features a robotic gripper arm for the transport of vials, driven by four stepper motors to ensure the highest precision and flexibility for the transport movement.

The default configuration of the analytical head and sample loop allows an injection range from 0.1

0.1

– 100 μL for the 1260 Infinity II Vialsamplers. An analytical head with extended volume is also available for injection volumes from 0.1 can be operated at up to 400 volume can even reach 1800

The 2-position/6-port injection valve is driven by a high-speed, hybrid stepper motor, ensuring smooth switching between the mainpass and bypass positions. Thanks to the flow-through design of the Vialsampler, the eluent continuously rinses the component parts of the sample introduction system. This, together with the automated needle wash function for rinsing the outer surface of the needle, ensures the lowest level of carryover for each analysis. For advanced delay volume reduction, the injection valve can be programmed to automatically switch back to the bypass position when the injection is completed.

Agilent InfinityLab LC Series Vialsamplers User Manual 16

– 20 μL for the 1290 Infinity II Vialsampler and

– 900 μL, and bar. With the Multidraw Kit, the maximum injection μL.

1Introduction

Overview of the Module

The Agilent InfinityLab Integrated Column Compartment, an optional upgrade for the Vialsampler, eliminates the need for having a standalone column thermostat in the LC stack. It has the heating capability of reaching temperatures up to 80 and can hold a total of two columns with maximum lengths of 30

For applications requiring control over the vial temperature, the Vialsampler can be equipped with the Agilent InfinityLab Sample Thermostat (G7167-60101). It features a vapor-compression refrigeration system and an electric heater, allowing the Vialsampler to reach vial temperatures down to 4

cm.

°C and up to 40 °C.

°C

Agilent InfinityLab LC Series Vialsamplers User Manual 17

1Introduction

Operating Principle

Sampling Sequence

The Vialsampler processor continuously monitors the movements of the vialsampler components during the sampling sequence. The processor defines specific time windows and mechanical ranges for each movement. If a specific step of the sampling sequence can’t be completed successfully, an error message is generated.

During the sampling sequence, the solvent bypasses the vialsampler via the injection valve. The gripper arm selects the sample vial, either from a static sample rack, or from external vial positions. The gripper arm places the sample vial below the injection needle. The required volume of sample is drawn into the sample loop by the metering device. Sample is applied to the column when the injection valve returns to the mainpass (main path) position at the end of the sampling sequence.

The sampling sequence occurs in the following order:

1 The injection valve switches to the bypass position. 2 The piston of the metering device moves to the initialization position. 3 The gripper arm moves from the home position, and selects the vial. At the

same time, the needle lifts out of the seat.

4 The gripper moves into the needle station and stops in the draw position. 5 The needle lowers into the vial. 6 The metering device draws the defined sample volume. 7 The needle lifts out of the vial.

8 The gripper arm moves out slightly and stops in the wash position1. 9 The needle moves downwards and dips into the wash well of the wash port.

Simultaneously the peristaltic pump delivers the flush solvent.

10 The needle moves back. 11 The gripper arm moves out of the needle station and the wash port snaps

back in position.

only if automated needle wash is selected. If this feature is disabled, the gripper arm positions the sample vial directly below the needle (Step 4) and lowers the needle into the vial.

Agilent InfinityLab LC Series Vialsamplers User Manual 18

1Introduction

Vial

Wash port

Needle seat

Sampling loop

Solvent

Wash

pump

Metering device

Operating Principle

12 The gripper arm replaces the vial, and returns to the home position.

Simultaneously, the needle lowers into the seat.

13 The injection valve switches to the mainpass (main path) position.

Injection Sequence

Before the start of the injection sequence, and during an analysis, the injection valve is in the mainpass (main path) position (see position, the mobile phase flows through the autosamplers metering device, sample loop, and needle. This ensures that all parts in contact with sample are flushed during the run, thus minimizing carryover.

Figure 4 on page 19). In this

Figure 4 Mainpass (main path) Position - standard position during runs and when the sampler

idle

Agilent InfinityLab LC Series Vialsamplers User Manual 19

1Introduction

Vial

Wash port

Needle seat

Sampling loop

Solvent

Wash pump

Metering device

Operating Principle

When the sample sequence begins, the valve unit switches to the bypass position (see

Figure 5 on page 20). Solvent from the pump enters the valve unit at

port 1, and flows directly to the column through port 6.

Figure 5Valve in bypass - needle in vial, metering device aspirates sample volume

Agilent InfinityLab LC Series Vialsamplers User Manual 20

1Introduction

Vial

Wash

port

Needle seat

Sampling loop

Solvent

Wash pump

Metering device

Operating Principle

Then the vial is positioned below the needle. The needle moves down into the vial, the metering unit draws the required sample volume into the loop, and the needle is raised. In the next step, the needle is washed (see

Figure 6 on page 21).

Figure 6 Outer face of needle getting washed in wash port

Agilent InfinityLab LC Series Vialsamplers User Manual 21

1Introduction

Vial

Wash port

Needle seat

Sampling loop

Solvent

Wash pump

Metering device

Operating Principle

When the metering unit has drawn the required volume of sample into the sample loop, the vial is replaced in the sample tray. The wash port flips into the origin position, the needle is lowered into the needle seat, and the injection valve switches back to mainpass (main path) position, flushing the sample onto the column (see

Figure 7 on page 22).

Figure 7 Valve switches to mainpass (main path) - sample is transferred towards the LC column

Agilent InfinityLab LC Series Vialsamplers User Manual 22

1Introduction

Operating Principle

Needle Parkstation

The needle parkstation comprises two main assemblies: needle drive and wash port.

Figure 8Needle Station

Needle-Drive

The needle movement is driven by a stepper motor connected to the spindle assembly by a toothed belt. The circular motion of the motor is converted to linear motion by the drive nut on the spindle assembly. The upper and lower needle positions are detected by reflection sensors on the needle station board, while the needle-in-vial position is determined by counting the motor steps from the upper needle-sensor position.

Agilent InfinityLab LC Series Vialsamplers User Manual 23

1Introduction

NOTE

Operating Principle

Hydraulic Box

The hydraulic box comprises two main assemblies: metering device, and injection valve.

Figure 9 Hydraulic Unit

The replacement hydraulic box excludes the injection valve and metering head assemblies.

Agilent InfinityLab LC Series Vialsamplers User Manual 24

1Introduction

Operating Principle

Analytical Head

The analytical head is driven by the stepper motor that is connected to the drive shaft by a toothed belt. The drive nut on the spindle converts the circular movement of the spindle to linear motion. The drive nut pushes the sapphire piston against the tension of the spring into the analytical head. The base of the piston sits on the large bearing of the drive nut, which ensures the piston is always centered. A ceramic ring guides the movement of the piston in the analytical head. The home position of the piston is sensed by an optical sensor on the hydraulic unit board while the sample volume is determined by counting the number of steps from the home position. The backward movement of the piston (driven by the spring) draws sample from the vial.

Injection Valve

The two-position 6-port injection valve is driven by a stepper motor. Only five of the six ports are used (port 3 is not used). A lever/slider mechanism transfers the movement of the stepper motor to the injection valve. Two microswitches monitor switching of the valve (bypass and mainpass (main path) end positions). No valve adjustments are required after replacing internal components.

Agilent InfinityLab LC Series Vialsamplers User Manual 25

1Introduction

X motor

Theta motor

Gripper motor

Gripper arm

Z motor

Flex board

Theta axis

X axis

Z axis

Operating Principle

Transport Assembly

The transport unit comprises an X-axis slide (left-right motion), a Z-axis arm (up-down motion), and a gripper assembly (rotation and vial-gripping).

Figure 10 Transport Assembly

The transport assembly uses four stepper motors driven in closed-loop mode for accurate positioning of the gripper assembly. The rotational movement of the motors is converted to linear motion (X- and Z-axes) by toothed belts connected to the drive spindles. The rotation (theta axes) of the gripper assembly is transferred from the motor by a toothed belt and series of gears. The opening and closing of the gripper fingers are driven by a stepper motor linked by a toothed belt to the planetary gearing inside the gripper assembly.

The stepper motor positions are determined by the optical encoders mounted onto the stepper-motor housing. The encoders monitor the position of the motors continually, and correct for position errors automatically (e.g. if the gripper is accidentally moved out of position when loading vials into the vial tray). The initialization positions of the moving components are sensed by reflection sensors mounted on the flex board. These positions are used by the processor to calculate the actual motor position. An additional six reflection sensors for tray recognition are mounted on the flex board at the front of the assembly.

Agilent InfinityLab LC Series Vialsamplers User Manual 26

1Introduction

Leak and Waste Handling

The Agilent InfinityLab LC Series has been designed for safe leak and waste handling. It is important that all security concepts are understood and instructions are carefully followed.

The solvent cabinet is designed to store a maximum volume of 8 L solvent. The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 2 Cabinets (a printed copy of the guideline has been shipped with the solvent cabinet, electronic copies are available on the Internet).

All leak plane outlets are situated in a consistent position so that all Infinity and Infinity II modules can be stacked on top of each other. Waste tubes are guided through a channel on the right hand side of the instrument, keeping the front access clear from tubes.

The leak plane provides leak management by catching all internal liquid leaks, guiding them to the leak sensor for leak detection, and passing them on to the next module below, if the leak sensor fails. The leak sensor in the leak plane stops the running system as soon as the leak detection level is reached.

Solvent and condensate is guided through the waste channel into the waste container:

• from the detector's flow cell outlet

• from the Multisampler needle wash port

• from the Sample Cooler or Sample Thermostat (condensate)

• from the pump's Seal Wash Sensor (if applicable)

• from the pump's Purge Valve or Multipurpose Valve

L. For details, see the usage guideline for the Agilent Infinity II Solvent

Agilent InfinityLab LC Series Vialsamplers User Manual 27

1Introduction

Leak and Waste Handling

Figure 11 Infinity II Leak Waste Concept (Flex Bench installation)

Agilent InfinityLab LC Series Vialsamplers User Manual 28

1Introduction

Leak and Waste Handling

Figure 12 Infinity II Single Stack Leak Waste Concept (bench installation)

Agilent InfinityLab LC Series Vialsamplers User Manual 29

1Introduction

Leak and Waste Handling

Figure 13 Infinity II Two Stack Leak Waste Concept (bench installation)

The waste tube connected to the leak plane outlet on each of the bottom instruments guides the solvent to a suitable waste container.

Agilent InfinityLab LC Series Vialsamplers User Manual 30

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