Ordering
Table 6 contains details for
ordering instrumentation and
supplies.
Amini acid standards
Each amino acid standard contains the following amino acids:
Asp Aspartic acid
Glu Glutamic acid
Ser Serine
His Histidine
Gly Glycine
Thr Threonine
Ala Alanine
Arg Arginine
Tyr Tyrosine
Cys-SS-Cys Cystine
Val Valine
Met Methionine
Phe Phenylalanine
Ile Isoleucine
Leu Leucine
Lys Lysine
Pro Proline
Order Number Description
Instrumentation
G1312A Agilent 1100 Series binary pump
G1312-67301 0.12 mm id capillary
or
A-330 Upchurch semi prep filter 5022-2165
G1313A Agilent 1100 Series autosampler
#011 Vial kit
or
G1327A Agilent 1100 Series thermostatted autosampler
#011 Vial kit
Optimum performance of the thermostatted autosampler requires the following capillary connections:
G1312-67305 (Pump to autosampler) Outlet capillary
5021-1823 (Autosampler to column compartment) Flexible tubing 400 mm, 0.12 mm id (no fittings)
G1316-87303 (Column compartment to inlet column) Capillary, 70 mm, 0.12 mm id
5021-1823 (Outlet column to DAD) Flexible tubing 400 mm, 0.12 mm id (no fittings)
G1322A Agilent 1100 Series vacuum degasser
G1316A Agilent 1100 Series column compartment
G1315A Agilent 1100 Series diode-array detector
#018 Standard flow cell (10 mm, 13 µl)
G1321A Agilent 1100 Series fluorescence detector
G1319A Agilent ChemStation
Supplies
5063-6588 Amino Acid Separation Kit contains:
79916AA-572 Column for amino acid analysis, 200 × 2.1 mm
79916KT-110 Guard columns, Hypersil ODS, 20 × 2.1 (3 per pack)
79900CH-010 Guard cartridge holder
79841-87609 Column connector, 35 mm, 0.12 mm id.
9301-1388 Crimpcap microvials (100 per pack)
5181-1210 Crimpcaps, butyl rubber (100 per pack)
Amino Acid Standards
5061-3330 1 nmol/µl AA standard 10 × 1 ml ampules
5061-3331 250 pmol/µl AA standard 10 × 1 ml ampules
5061-3332 100 pmol/µl AA standard 10 × 1 ml ampules
5061-3333 25 pmol/µl AA standard 10 × 1 ml ampules
5061-3334 10 pmol/µl AA standard 10 × 1 ml ampules
5062-2478 Supplemental Amino Acid Kit includes 1 g each norva
line, sarcosine, asparagine, glutamine, tryptophan
and 4-hydroxyproline
Reagents for amino acid analysis
5061-3335 OPA reagent, 10 mg/ml each in 0.4 M borate buffer
o-phthalaldehyde (OPA) and 3-mercaptoproprionic
acid, 6 ampules
5061-3337 FMOC reagent, 2.5 mg/ml in acetonitrile, 9-fluorenyl
methylchloroformate, 1 ml
5061-3339 Borate buffer, 100 ml
5062-2479 DTDPA (dithiodipropionic) reagent for analysis of
cysteine, 5 g
Software
G1300-10013 Software for special method set up and reporting—
software description included as document file
(User contributed software)
5968-5796E Sensitive and reliable amino acid analysis in protein
hydrolysates using the Agilent 1100 Series HPLC
Support
(not included in kit
)
H1170A Application Optimization Service—onsite, including
travel from 1–10 continuous days
Table 6
Ordering information