3B SCIENTIFIC PHYSICS 100 Instruction Manual

3B SCIENTIFIC® PHYSICS

1

Digital Course Microscope Model 100, LED with built-in Camera

115 V, 50/60 Hz: 1005403 / 230 V, 50/60 Hz: 1005404

Instruction Manual

07/13 ALF

1 Eyepiece
2 Tube
3 Camera
4 USB connection
5 Stand
6 Revolver with objectives
7 Lock screw for object stage
8 Specimen clips
9 Object stage
10 Condenser with iris dia-

phragm and filter holder

11 Adjustment knob for coarse

focusing

12 Adjustment knob for fine

focusing
13 Lamp housing
14 Switch with illumination

control

1. Description, technical data

The digital course microscope with built-in cam­era allows two-dimensional viewing of objects
(thin sections of plant or animal specimen) in
40x to 400x magnification. It also allows photo­graphic or video-recording documentation of
images.

The microscope 1005403 is for operation with a
mains voltage of 115 V (±10%), and the
1005404 unit is for operation with 230 V (±10%).

As well as real-time video playback, single im­ages, sequences and video recording, the
Photolib software provides a wide range of func­tions for the presentation, processing and
evaluation of images.

The installation CD contains a detailed descrip­tion of the software in English, and additional
advice and assistance is available in the help

files of the software.
Stand: All-metal stand, arm firmly connected

with base, pinion knobs attached on both sides
of the stand for coarse and fine focusing

Tube: Monocular inclined 45°, head rotation
360°

Eyepiece: Widefield eyepiece WF 10x 18 mm
with pointer

Objectives: Revolver with 3 DIN achromatic
objectives 4x / 0.10, 10x / 0.25, 40x / 0.65 (with
specimen protection)

Magnification: 40x, 100x, 400x
Object stage: 110 x 120 mm

2

with 2 specimen

clips
Illumination: Adjustable LED lighting incorpo-

rated into the base with a focussing lens in the
lighting shaft, power supplied by rechargeable
battery, 115 V or 230 V, 50/60 Hz charger

2

Condenser: Bright-field condenser N.A. 0.65,
iris diaphragm, filter holder and blue filter

Camera sensor: 1/3” CMOS, 1.3 Mpixel, colour
prints

Power supply: via USB 2.0
System requirements: WIN2000, WINXP,

Vista, WIN7 and WIN8

Dimensions: 175 x 135 x 335 mm³ approx.
Weight: 3.1 kg approx.

2. Unpacking and assembly

The microscope is packed in a molded styro­foam container.

• Take the container out of the carton remove

the tape and carefully lift the top half off the
container. Be careful not to let the optical
items (objectives and eyepieces) drop down.

• To avoid condensation on the optical com-

ponents, leave the microscope in the original
packing to allow it to adjust to room tem­perature.

• Using both hands (one around the pillar and

one around the base), lift the microscope
from the container and put it on a stable
desk.

• The objectives will be found within individual

protective vials. Install the objectives into the
microscope nosepiece from the lowest
magnification to the highest, in a clockwise
direction from the rear.

• Insert the eyepiece into the tube.

3. Operation

3.1 General information

• Set the microscope on a level table.

• Place the object to be observed in the center

of the object plate. Use the clips to fasten it
into place. Make certain that the specimen is
centered over the opening in the stage.

• Connect the mains adaptor, and switch on

the illumination.

Alternatively, the microscope can also be oper­ated without being connected to the mains.

• Make certain that the specimen is centered

over the opening in the stage.

• To obtain a high contrast, adjust the back-

ground illumination by means of the iris dia­phragm and the variable illumination control.

• Rotate the nosepiece until the objective with

the lowest magnification is pointed at the
specimen. There is a definite “click” when
each objective is lined up properly.

NOTE: It is best to begin with the lowest power
objective. This is important to reveal general
structural details with the largest field of view
first. Than you may increase the magnification
as needed to reveal small details.

To determine the magnification at which you are
viewing a specimen, multiply the power of the
eyepiece by the power of the objective.

• Adjust the coarse-focusing-knob which

moves the stage up until the specimen is fo­cused. Be careful that the objective does not
make contact with the slide at any time. This
may cause damage to the objective and/or
crack your slide.

• Adjust the fine-focusing-knob to get the im-

age more sharp and more clear.

• Colour filters may be inserted into the filter

holder for definition of specimen parts.
Swing the filter holder out and insert colour
filters.

• Always turn off the light immediately after

use.

• Be careful not to spill any liquids on the mi-

croscope.

• Do not mishandle or impose unnecessary

force on the microscope.

• Do not wipe the optics with your hands.

• Do not attempt to service the microscope

yourself.

3.2 Installation of the software

• Insert the installation CD into the computer’s

CD drive.

• Follow the installation instructions (see also

the description of the software on the instal­lation CD).

3.3 Displaying images on the computer

• Connect the microscope to the computer

using the USB cable.

• Start up the software.

• After clicking on the camera icon in the tool-

bar, the image of the specimen will appear
on the computer screen.

• If necessary, re-adjust the brightness and

contrast using the iris diaphragm and the va­riable illumination control.

• Adjust the sharpness of the image by means

of the focusing knobs of the microscope.

• If necessary, adjust the settings of the ca-

mera in the video window according to the
particular requirements.

• For further work using the software, see the

instructions for the software that are on the
installation CD and the help files in the soft­ware.

3B Scientific GmbH • Rudorffweg 8 • 21031 Hamburg • Germany • www.3bscientific.com

Subject to technical amendments

© Copyright 2013 3B Scientific GmbH

4. Storage, cleaning and disposal

• Keep the microscope in a clean, dry and

dust free place.

• When not in use always cover the micro-

scope with the dust cover.

• Do not expose it to temperatures below 0°C

and above 40°C and a max. relative humid­ity of over 85%.

• Always unplug the mains plug before clean-

ing or maintenance.

• Do not clean the unit with volatile solvents or

abrasive cleaners.

• Do not disassemble objective or eyepieces

to attempt to clean them.

• Use a soft linen cloth and some ethanol to

clean the microscope.

• Use a soft lens tissue to clean the optics.

• The packaging should

be disposed of at local
recycling points.

• Should you need to

dispose of the equip­ment itself, never throw
it away in normal do­mestic waste. Local
regulations for the dis­posal of electrical
equipment will apply.

• Do not dispose of the battery in the regular

household garbage. Follow the local regula­tions (In Germany: BattG; EU: 2006/66/EG).