Nikon LV150, LV150A User Manual

M350 E 05.4.NF.1

Industrial Microscope

ECLIPSE LV150/LV150A

Instructions

Thank you for purchasing the Nikon products.

This instruction manual has been prepared for the users of Nikon’s industrial microscope “ECLIPSE LV150/LV150A.”

To ensure correct usage, read this manual carefully before operating the instrument.

•It is prohibited to reproduce or transmit this manual in part or whole without Nikon’s expressed permission.

•The contents of this manual are subject to change without notice.

•Although every effort has been made to ensure the accuracy of this manual, if you note any points that are unclear or incorrect, contact your nearest Nikon representative.

•Some of the products described in this manual may not be included in the set you have purchased.

•Be sure to read the instruction manual for any other products that may be used in combination with the microscope.

Warning/Caution Symbols Used in This Manual

Although Nikon products are designed to provide you with the utmost safety during use, incorrect usage or disregard of the instructions can cause personal injury or property damage. For your safety, read this instruction manual carefully and thoroughly before using the instrument. Do not discard this manual, but keep it near the product for easy reference.

In this manual, safety instructions are indicated with the symbols shown below. Be sure to follow the instructions indicated with these symbols to ensure correct and safe operation.

	Symbol			Meaning
		WARNING		Disregarding instructions marked with this symbol may lead
				to death or serious injury.
		CAUTION		Disregarding instructions marked with this symbol may lead
				to injury or property damage.

Meaning of Symbols Used on the Equipment

Symbol Meaning

Caution for heat.

This marking on the rear of the lamphouse, and near the lamphouse clamp screw on the illuminator (LV-UEPI and LV-UEPI2), calls your attention on the following.

For the symbol position, see pages 10 and 12.

•The lamphouse is very hot during and immediately after illumination.

•Risk of burns. Do not touch the lamphouse during and immediately after illumination.

•Make sure that the lamphouse has sufficiently cooled before replacing the lamp.

1

WARNING

1.Intended product use

This microscope should only be used for microscopic observation. Do not use it for any other purpose. Do not observe such a large sample as to stick out of the stage.

2.Do not disassemble.

Disassembly may cause malfunction, electrical shock, and/or injury. Any injury or damage due to such an act will not be warranted. Do not disassemble any part other than those described in this manual. If you experience any problem with the microscope, notify your nearest Nikon representative.

3.Read the instruction manuals carefully.

For your safety, carefully read this manual and the manuals provided with the other products to be used with the system. Be sure to read warnings and cautions at the beginning of each manual in particular.

When the external light source is used:

When you use the external light source using a mercury lamp or so on, handle the lamp with extreme caution. Read the manual for the light source carefully and observe handling precautions.

4.Ratings of power supply

The power circuit in this instrument is rated for AC power supplies of 100 to 240 V, 50/60 Hz. When connecting the instrument to a power line, check that the line conforms to the voltage and frequency ratings mentioned above.

Use of a power line that does not satisfy the ratings may lead to equipment malfunction or damage or a fire.

5.Power cord

Use only the supplied power cord. Using the wrong power cord could result in damage or a fire. Also, connect the microscope to a PE (protective earth) terminal, since the microscope complies with the electric shock protection class I.

And besides, to prevent electrical shock, always turn off the power switch (flip it to “” side) before connecting or disconnecting the power cord.

For details about the specified power cord, see “VIII. Specifications.”

6.Specified light source

This microscope must be used with a specified light source. The following light source combinations are specified for this microscope.

●Illuminator:

Nikon LV-UEPI Universal Epi Illuminator (model LV-UEPI) or Nikon LV-UEPI2 Universal Epi Illuminator (model LV-UEPI2)

●Lamphouse:

Nikon LV-LH50PC precentered lamphouse 12V 50W (model LV-LH50PC)

●Lamp:

Nikon LV-HL50W 12V 50W LONGLIFE halogen lamp (model LV-HL50W), or nonNikon 12V 50W SHORTLIFE halogen lamp (model OSRAM HLX 64610, OSRAM HLX 64611, or PHILIPS 7027)

If you wish to buy these lamps, contact your nearest Nikon representative.

2

WARNING

7.Light source other than the specified ones

To perform the epi-fl microscopy wit the LV-UEPI2 illuminator, the specified light source brightness may be less than the desired brightness. In this case, a light source other than the specified ones, an external light source, can be used for the LV-UEPI2.

Use the X-Cite 120 (manual type) or X-Cite 120PC (motorized type) manufactured by EXFO Electro-Optic Engineering Inc. for the external light source. In particular, when the LV150A is used for the microscope main body, be sure to attach the X-Cite 120PC to prevent a flash of light. The X-Cite 120PC must be connected with the LV150A through the RS-232C cable attached to the light source. When the LV150 is used, either external light source will work.

Please take note that if a light source other than the specified ones are installed onto this microscope, this microscope system will not be treated as a TUV/SEMI approved product.

8.Heat from the light source

The lamp and the lamphouse become extremely hot. To avoid burns, do not touch the lamphouse while the lamp is lit or for thirty minutes after it is turned off. Furthermore, to avoid the risk of fire, do not place fabric, paper, or highly flammable

volatile materials (such as gasoline, petroleum benzine, paint thinner, or alcohol) near the lamphouse while the lamp is lit or for about thirty minutes after it is turned off.

9.Air vents

Do not block the air vents on the microscope and lamphouse.

If the air vents are blocked, the temperature of the microscope will raise. And it results in damage or fire.

10.Ultraviolet light from a light source other than the specified ones

If you use a light source other than the specified ones and that has a mercury lamp or so on, the light source radiates ultraviolet light that is harmful to the eyes and skin from the emission port. Direct viewing of light from these lamps may result in snow blindness at a light case or blindness at worst. To prevent injury, follow the guidelines below.

1)Insert the UV collector lens into the optical path of the microscope unless the UV excitation light is necessary.

On the illuminator LV-UEPI2, the UV filter automatically enters the optical path when turning the microscopy selection knob to BF (bright-field) or DF (dark-field). The UV filter is removed from the optical path when turning the knob to FL1 (epi-fl 1) or FL2 (epi-fl 2).

2)When performing the epi-fl microscopy by using the UV excitation light, attach the filter cube dedicated to the UV excitation light. And then, if you must see the objective or its surroundings, be sure to see through the ultraviolet light shield.

3)Attach the light source to the microscope during use.

Always attach the light source to the microscope when the light source is ready to turn on. Do not turn on the light source unattached to the microscope, or remove the light source from the microscope while the light source is lit. When removing the light source from the microscope, turn off the power to the light source, and then unplug the power code from the wall outlet.

11.Reflection

Lustrous samples reflect the illumination. Do not observe the illuminated surface of a sample for a long time because the strong reflection may hurt your eyes. When you use the illuminator LV-UEPI2, be sure to view it through the ultraviolet light shield.

3

CAUTION

1.Handle the system gently

Components of this system are precision optical instruments. Handle them carefully, and do not subject them to any shocks.

The precision of the objectives in particular can be adversely affected even by weak shocks.

2.Do not wet the microscope

If the microscope gets wet, a short circuit may cause malfunction or abnormal heating of the microscope. If you accidentally spill water on the microscope, immediately turn off the power switch (flip it to the “” side) and unplug the power cord from the wall outlet. Then, wipe away the moisture using a dry cloth or the like. If water gets inside the microscope, do not use it; instead, notify your nearest Nikon representative.

3.Weak electromagnetic waves

This microscope emits weak electromagnetic waves. The accuracy of any precision electronic equipment may be adversely affected if positioned too close. If the microscope affects TV or radio reception, move the radio or TV farther away from the microscope.

4.Installation location

Being a precision optical instrument, the microscope may get damaged or loose accuracy if it is used or stored under unsuitable conditions. When selecting the installation location, note the following:

•Avoid a brightly lit location, such as exposed to direct sunlight or directly under a room light. The image quality deteriorates if there is excessive ambient light.

Always install the microscope with a surrounding clear area of 10 cm or more.

•Choose a location that is free from dust or dirt.

•Choose a flat surface with little vibration.

•Choose a sturdy desk or table that is able to bear the weight of the instrument.

•Do not install the microscope in a hot or humid location.

•Select a layout that allows easy removal of the power cord from the microscope’s AC inlet in the event of an emergency.

•For details about the operating environment and storage environment, see “VIII. Specifications.”

•Take enough space around the microscope referring to the layout diagrams on page 6.

•The microscope may be moved by earthquakes. We recommend taking anti-earthquake measures.

For details about the anti-earthquake measures, see “15. Countermeasures for Earthquakes” in “IV. Assembly.”

5.Cautions on moving the microscope

•The microscope is a precision optical instrument. Handle it carefully and do not subject it to a strong physical shock. (In particular, objectives may loose accuracy when exposed to even a weak physical shock.)

•When moving the microscope, first remove the stage and the lamphouse. Then, securely hold the microscope by the root of the arm from the back.

(Information) The microscope with the stage, eyepiece tube, lamphouse, and other parts attached, weighs approx. 20 kg.

•Do not hold the focusing knobs, eyepiece tube, lamphouse, sub-stage, etc., when carrying the microscope. They may come off and may cause serious injury or malfunction.

•Before carrying the stage, attach the fixing metals to hold the movement of the stage plate.

•Be careful not to pinch your fingers or hands during transportation.

4

CAUTION

6.Cautions on assembling the microscope

•Be careful not to pinch your fingers or hands during assembly.

•Scratches or fingerprints on the lens surface will adversely affect the microscope image. Be careful not to scratch or touch the lens surfaces.

7.Cautions on lamp replacement

•To prevent burn injury, allow the lamp to cool down sufficiently (for at least 30 minutes after it is turned off) before replacing the lamp.

•To prevent electrical shock and damage to the microscope, always turn off the power switch (flip it to the “” side) and unplug the power cord from the wall outlet before connecting or disconnecting the lamphouse.

•Do not touch the glass surface of the lamp with bare hands. Fingerprints or grease on the bulb surface will reduce the illumination intensity of the lamp. Wipe out any fingerprints or grease attached to the surface.

•Securely attach the lamphouse cover to the lamphouse after replacing the lamp. Never light the lamp while the lamphouse cover is open.

•When you dispose of the replaced lamp, do not break it up. Instead, dispose of the used lamp as special industrial waste or dispose of it according to the local regulations and rules.

8.Handing of filter cubes

When using the microscope configured with the illuminator LV-UEPI2, a filter cube can be attached to enable epi-fl microscopy. Note the following precautions for handling a filter cube.

•Interference filters (in particular, excitation filters exposed to intense light) are subject to aging. Replace them depending on their total operating hours.

•Filters can change in characteristics under high humidity. To avoid changes in characteristics and quality, do not use or store filters at high temperatures or high humidity, or expose them to rapid temperature changes. When not using filters, they should be stored with a drying agent in desiccators or sealed containers.

•The filters fitted in the nine types of filter cubes listed below have sharper wavelength characteristics than ordinary filters. However, these filters should be handled with care as they are applied with complicate coating. In particular, be cautious against wear during cleaning. (Observe the procedures described in “Cleaning Filters and Lenses” of “VII. Care and Maintenance.”)

Single-band filter cubes: DAPI, FITC, TxRed, and GFP Multi-band filter cubes: F-R, F-T, D-F, D-F-R, and D-F-T.

5

LAYOUT DIAGRAMS

100

643 (Stage area)

250

Center of gravity position

6 x 6 STAGE JAPAN

168

305

503 (Stage area)

570.4

Eye point	192.6	80

Center of gravity position

Center of
gravity position
508.5	484
230	230

250

79

262

Dimensions are in mm.

This illustration depicts the LV150A microscope configured with the LV-UEPI illuminator, LVTI3 eyepiece tube, LV-LH50PC lamphouse, and 6x6 stage.

6

OPERATING POSTURE

The figure below shows the operating posture that prevents strain on your body.

Choose a workbench and a chair having similar dimensions to those shown in the figure.

The 95th percentile male (Height: 189.5 cm)

of the eyepiece height point)	seating surface)
height	of the
1269 (The	(The height
	405

510

673

760

Dimensions are in mm.

660

*The height of the eye point is that when one eye-level riser is mounted on the microscope.

*Take at least 610 mm of horizontal clearance for your legs.

The 5th percentile female (Height: 147.5 cm)

of the eyepiece height point)	seating surface)
height	of the
1244 (The	(The height
	565

510

673

760

135

Dimensions are in mm.

660

* Take at least 610 mm of horizontal clearance for your legs.

7

CONTENTS
Warning/Caution Symbols Used in This Manual ..............................................		1
Meaning of Symbols Used on the Equipment ..................................................		2
WARNING .........................................................................................................		2
CAUTION ..........................................................................................................		4
LAYOUT DIAGRAMS ...........................................................................................		6
OPERATING POSTURE.......................................................................................		7
Names of Each Part ...................................................................................		10
1	When Configured with the Illuminator LV-UEPI ...........................................................	10
2	When Configured with the Illuminator LV-UEPI2 .........................................................	12
3	Rear View ........................................................................................................................	14
Microscopy .................................................................................................		15
1	Bright-Field Microscopy .................................................................................................	16
2	Dark-Field Microscopy ...................................................................................................	18
3	Differential Interference Contrast (DIC) Microscopy .....................................................	20
4	Simplified Polarization Microscopy ................................................................................	22
5	Sensitive Polarization Microscopy ..................................................................................	24
6	Epi-Fluorescence Microscopy .........................................................................................	25
Operation of Each Part ..............................................................................		26
1	Operation of the Illumination ..........................................................................................	26
2	Filters ...............................................................................................................................	26
3	Coarse/Fine Focus Knobs ................................................................................................	27
4	Eyepiece Tube .................................................................................................................	29
5	Diopter Adjustment .........................................................................................................	30
6	Interpupillary Distance Adjustment ................................................................................	30
7	Field Diaphragm ..............................................................................................................	31
8	Aperture Diaphragm ........................................................................................................	32
9	Illumination Selection Lever and Microcopy Selection Knob ........................................	33
10	Stage ................................................................................................................................	34
11	Motorized Nosepiece Operation ......................................................................................	35
12	Polarizer Slider ................................................................................................................	36
13	Lambda Plate Slider (for the LV-UEPI2 only) ................................................................	37
14	Analyzer Slider ................................................................................................................	38
15	DIC Slider .......................................................................................................................	39
16	Filter Cubes for Fluorescence Observation (for the LV-UEPI2 only) .............................	40
17	Excitation Light Balancer (for the LV-UEPI2 Only) .......................................................	43

8

Assembly ....................................................................................................		45
1	Attaching the Stage and the Holder .................................................................................	47
2	Assembling the Nosepiece ..............................................................................................	48
3	Attaching the Illuminator ................................................................................................	50
4	Attaching the Lamphouse and Replacing the Lamp .......................................................	53
5	Attaching the Fiver Adapter and External Light Source .................................................	54
6	Attaching the Eyepiece Tube ...........................................................................................	56
7	Attaching Eyepieces ........................................................................................................	56
8	Attaching Objectives .......................................................................................................	56
9	Attaching Eye Level Riser ..............................................................................................	57
10	Attaching Column Riser ..................................................................................................	57
11	Connecting the Power Cord ............................................................................................	58
12	Connecting the RS-232C .................................................................................................	58
13	Installing Separately Sold Accessories ............................................................................	58
14	Anti-static Treatment .......................................................................................................	58
15	Countermeasures for Earthquakes ...................................................................................	59
External Communications Control...........................................................		60
Troubleshooting.........................................................................................		66
1	Viewing and control systems ...........................................................................................	66
2	Electrical ..........................................................................................................................	69
Care and Maintenance ..............................................................................		70
1	Cleaning Lenses and Filters ............................................................................................	71
2	Cleaning the Painted, Plastic, and Printed Parts ..............................................................	71
3	Storage .............................................................................................................................	71
4	Regular Inspections .........................................................................................................	71
Specifications ............................................................................................		72

9

Names of Each Part

1 When Configured with the Illuminator LV-UEPI

Names of Parts

Vertical tube

Binocular part

Eyepiece tube LV-TI3

Illuminator LV-UEPI

Nosepiece

Objective

Lamphouse

IN	OUT
	100
0
	0
100

A.STOP

F.STOP

Filter sliders BF

DF “CAUTION for heat” symbol

Polarizer slider *1

Analyzer slider *1

DIC slider *2

Stage

6 x6 JAPANSTAGE

Power indicator

Main body of Tool holders the microscope

*1: For DIC microscopy or simplified polarization microscopy. *2: For DIC microscopy.

This drawing depicts the ECLIPSE LV150A microscope configured with the LV-UEPI illuminator, LV-TI3 eyepiece tube, LV-LH50PC lamphouse, 6x6 stage, and attachments for DIC microscopy.

10

I. Names of Each Part

Names of Operational Parts

Clamp screw for various adapters

Diopter adjustment ring

Eyepiece

Analyzer slider *1

Prism movement knob

Prism selection knob

DIC slider *2

Optical path selection lever

Field diaphragm open/close lever

Aperture diaphragm open/close lever

IN	OUT
	100
0
	0
100

A.STOP

F.STOP

BF	Filter sliders

DF

Field diaphragm centering screw

Polarizer slider *1

Bright/dark-field illumination selection lever

Stage coarse/fine movement selection switch

Stage coarse movement lever

Fine focus knob

Coarse focus knob

OBJ.

OFF

Brightness control knob

Coarse torque adjustment ring

Nosepiece rotation button (on LV150A only)

6 x6 JAPANSTAGE

Rotates the nosepiece counterclockwise (when seen from above the microscope).

Nosepiece rotation button (on LV150A only)

Rotates the nosepiece clockwise (when seen from above the microscope).

Stage fine movement knob for Y-axis

Stage fine movement knob for X-axis

Fine focus knob

Coarse focus stopper ring

*1: For DIC microscopy or simplified polarization microscopy. *2: For DIC microscopy.

This drawing depicts the ECLIPSE LV150A microscope configured with the LV-UEPI illuminator, LV-TI3 eyepiece tube, LV-LH50PC lamphouse, 6x6 stage, and attachments for DIC microscopy.

11

2 When Configured with the Illuminator LV-UEPI2

Names of Parts

Vertical tube

Eyepiece tube LV-TT2

Binocular part

Illuminator LV-UEPI2

Microscopy selection knob

Ultraviolet light shield

DIC slider *3

Stage

Lamphouse

IN	OUT
	100
0
	20
100
	LV-TT2

A.STOP

F.STOP

	BF		Filter sliders
DF		FL2	“CAUTION for heat” symbol
		S
	FL1		Polarizer slider *1
			Dummy slider *2
			Analyzer slider *1
			Nosepiece
			Objective
			6x6
			JAPANSTAGE

Power indicator

Tool holders

Main body of the microscope

*1: For DIC microscopy, simplified polarization microscopy, or sensitive polarization microscopy. *2: Lambda plate slider in case of sensitive polarization microscopy.

*3: For DIC microscopy.

This drawing depicts the ECLIPSE LV150A microscope configured with the LV-UEPI2 illuminator, LV-TT2 eyepiece tube, LV-LH50PC lamphouse, 6x6 stage, and attachments for DIC microscopy.

12

I. Names of Each Part

Names of Operational Parts

Clamp screw for various adapters

Diopter adjustment ring

Eyepiece

Optical path selection lever

Field diaphragm open/close lever

Aperture diaphragm open/close lever

IN	OUT
	100
0
	20
100
	LV-TT2

Microscopy selection knob

A.STOP

F.STOP

Prism movement knob

Prism selection knob

DIC slider *2

	BF
DF	FL2
	S
	FL1

6x6 JAPANSTAGE

Filter sliders

Aperture diaphragm centering screw (on both sides)

Field diaphragm centering screw (on both sides)

Polarizer slider *1

Dummy slider *2

Analyzer slider *1

Stage coarse/fine movement selection switch

Stage coarse movement lever

	Fine focus knob	Stage fine movement knob
	Coarse focus knob	for Y-axis
		Stage fine movement knob
		for X-axis
		Fine focus knob
	OBJ.
	OFF
		Coarse focus stopper ring
		Brightness control knob

Coarse torque adjustment ring

Nosepiece rotation button (on LV150A only)

Rotates the nosepiece counterclockwise (when seen from above the microscope).

Nosepiece rotation button (on LV150A only)

Rotates the nosepiece clockwise (when seen from above the microscope).

*1: For DIC microscopy, simplified polarization microscopy, or sensitive polarization microscopy. *2: Lambda plate slider in case of sensitive polarization microscopy.

*3: For DIC microscopy.

This drawing depicts the ECLIPSE LV150A microscope configured with the LV-UEPI2 illuminator, LV-TT2 eyepiece tube, LV-LH50PC lamphouse, 6x6 stage, and attachments for DIC microscopy.

13

I. Names of Each Part

3 Rear View

“CAUTION for heat” symbol

Caution label

CAUTION ! - High Temperature -

1. Do not touch the lamphouse while the lamp is lit. The surface of the lamphouse becomes hot when the lamp is on.

2. Turn off the power and allow the lamp and lamphouse to cool enough before replacing the lamp. Wait for at least 30 minutes after turning off

the lamp

3. Use 12V50W HALOGEN lamp only.

	HALOGEN 12V50W
	LV-LH50PC
JAPAN	6 5 2 7 0 1

Connector for connecting
the lamphouse
	LAMP
	DC12V 50W
	Input voltage indication
	ECLIPSE LV150A
	100–240V~ 1.2A 50/60Hz
	MADE IN JAPAN
	5 1 0 0 0 1
	TÜV
	including interference that may cause
	undesired operation.
	This Class A digital apparatus complies with
	Canadian ICES-003.
	Cet appareil numérique de la classe A est
	confirme à la norme NMB-003 du Canada.
Grounding tap (M4)	Power switch
	RS232C

RS232C connector

AC inlet

This drawing depicts the ECLIPSE LV150A microscope configured with the LV-UEPI illuminator, LV-TI3 eyepiece tube, LV-LH50PC lamphouse, and 6x6 stage.

14

Microscopy

This chapter describes the procedures for each microscopy.

This microscope can be configured with two types of illuminators, LV-UEPI or LV-UEPI2. See the table below for the microscopies available with each illuminator, as well as the optional accessories required for each microscopy.

●If the microscope has not yet been assembled, see “IV. Assembly” on p.45 first.

●See “III. Operation of Each Part” on p.26 for how to operate each part of the microscope.

Microscope	Procedure	Illuminators	Required accessories (optional)
Bright-field	p.16 to 17	LV-UEPI	–
microscopy		LV-UEPI2
Dark-field	p.18 to 19	LV-UEPI	BD objective
microscopy		LV-UEPI2	BD quintuple nosepiece, universal quintuple nosepiece
			or motorized universal quintuple nosepiece*
			(The standard sextuple nosepiece cannot be used
			for dark-field microscopy.)
Differential	p.20 to 21	LV-UEPI	Polarizer
interference		LV-UEPI2	Analyzer
contrast (DIC)			DIC slider
microscopy			Universal quintuple nosepiece or motorized universal
			quintuple nosepiece*
			Objectives marked “LU”
			(Objectives marked “LU” are suitable for DIC
			microscopy.)
Simplified	p.22 to 23	LV-UEPI	Polarizer
polarization		LV-UEPI2	Analyzer
microscopy
Sensitive	p.24	LV-UEPI2	Polarizer
polarization			Lambda plate
microscopy			Analyzer
Epi-	p.25	LV-UEPI2	Filter cube
fluorescence			(Up to two cubes can be attached.)
microscopy			Fluorescence excitation light balance filter (optional)

* For the LV150A only.

15

1 Bright-Field Microscopy

When configured with the LV-UEPI

1.Turn on the power.

2.Set the microscope for bright-field microscopy

If accessories for DIC microscopy (*1 to *3) are in place, pull them out of the optical path.

3.Place the sample on the stage and focus on it.

(p.27)

4.Adjust the diopter. (p.30)

5.Adjust the interpupillary

distance. (p.30)

6.Change the magnification and observe the sample.

Hint:

It may be difficult to focus on a sample with small contrast, such on a polished surface. In a case like this, stop down the field diaphragm so that its image can be seen in the viewfield, and try to focus on the rim of the diaphragm image. When the rim is in focus, the sample is in focus just as well.

1Push in.

Binocular eyepiece: 100% (P.29)

2	Push in.	*1
	BF (bright-field) (p.33)
	Select the	*2
3	10x objective.
	On the LV150A,
	use the nosepiece
	rotation buttons. (p.35)
4	Lower the stage
	as far as it will go.
	Coarse focus knob
	(p.27)
5	Adjust the
	brightness.

Brightness control knob (p.26)

Select the

1 10x objective.

On the LV150A, use the nosepiece rotation buttons.

(p.35)

Finely adjust 2 the focus.

Coarse/fine focus knob (p.27)

Adjust the

3brightness.

Brightness control knob (p.26)

A STOP

F.STOP

BF

DF

A.STOP

F.STOP

BF

DF

Raise the levers. 6

To fully open the field and aperture diaphragms.

(p.31 and p.32)

Push in the NCB11 filter. 7

To compensate color temperature.

(p.26)

	Adjust the
*3	brightness.	8

ND filter (p.26)

Power switch

Adjust to circumscribe the viewfield. 4

Field diaphragm (p.31)

Image of field diaphragm

Viewfield

Adjust to 70 to 80% of the objective’s N.A. 5

Aperture diaphragm (p.32)

Objective’s pupil

Image of aperture diaphragm

Adjust the brightness. 6

ND filter (p.26)

16

II. Microscopy

When configured with the LV-UEPI2

1.Turn on the power.

2.Set the microscope for bright-field microscopy.

If accessories for DIC or polarization microscopy (*1 to *3) are in place, pull them out of the optical path.

3.Place the sample on the stage and focus on it.

(p.27)

4.Adjust the angle of the

binocular part.

(p.29)

5.Adjust the diopter. (p.30)

6.Adjust the interpupillary

distance. (p.30)

7.Change the magnification and observe the sample.

Hint:

It may be difficult to focus on a sample with small contrast, such on a polished surface. In a case like this, stop down the field diaphragm so that its image can be seen in the viewfield, and try to focus on the rim of the diaphragm image. When the rim is in focus, the sample is in focus just as well.

1 Push in.

Binocular eyepiece: 100% (P.29)

Turn the microscopy

2 selection knob.

BF (bright-field) (P.33)			BF
		DF	FL2
			FL1
Select the	*2

310x objective.

On the LV150A,

use the nosepiece rotation buttons. (p.35)

Lower the stage

4as far as it will go.

Coarse focus knob (p.27)

Adjust the

5brightness.

Brightness control knob (p.26)

	Select the desired		BF
1		DF	FL2
	magnification.		FL1
	On the LV150A,
	use the nosepiece
	rotation switch.
	(p.35)
2	Finely adjust
	the focus.
	Coarse/fine focus
	knob (p.27)
3	Adjust the
	brightness.

Brightness control knob (p.26)

A.STOP

F.STOP

A.STOP

F.STOP

Raise the levers. 6

To fully open the field and aperture diaphragms.

(p.31 and p.32)

	Push in the
	NCB11 filter.	7
	To compensate
	color temperature.
	(p.26)
	Adjust the
*3	brightness.	8
*1	ND filter
	(p.26)

Power switch

Adjust to circumscribe the viewfield. 4

Field diaphragm (p.31)

Image of field diaphragm

Viewfield

Adjust to 70 to 80% of the objective’s N.A. 5

Aperture diaphragm (p.32)

Objective’s pupil

Image of aperture diaphragm

Adjust the brightness. 6

ND filter (p.26)

17

2 Dark-Field Microscopy

When configured with the LV-UEPI

1.Mount BD objectives and a BD quintuple nosepiece, universal quintuple nosepiece, or motorized universal quintuple nosepiece. (p.56 and 49)

The standard sextuple nosepiece cannot be used for dark-field microscopy.

2.Focus on the sample with bright-field microscopy. (p.16)

3.Set the microscope for dark-field microscopy.

The field and aperture diaphragms are fully opened automatically. (However, the lever positions are not changed.)

1Pull out.

DF (dark-field) (P.33)

A.STOP

F.STOP

BF

DF

Adjust the brightness. 3

ND filter (p.26)

Adjust the 2 brightness.

Brightness control knob (p.26)

4.Return the microscope to bright-field microscopy.

1 Push in.

BF (bright-field) (p.33)

BF DF

The field and aperture diaphragms automatically return to what they were before the microscope was set to darkfield microscopy.

A.STOP

F.STOP

Adjust the brightness. 3

ND filter (p.26)

Adjust the

2brightness.

Brightness control knob (p.26)

18

II. Microscopy

When configured with the LV-UEPI2

1.Mount BD objectives and a BD quintuple nosepiece, universal quintuple nosepiece, or motorized universal quintuple nosepiece. (p.56 and 49)

The standard sextuple nosepiece cannot be used for dark-field microscopy.

2.Focus on the sample with bright-field microscopy. (p.17)

3.Set the microscope for dark-field microscopy.

Turn the microscopy

1selection knob.

DF (dark-field) (p.33)

	DBF
FL2DF	FL2BF
	S
	FL1

The field and aperture diaphragms are fully opened automatically. (However, the lever positions are not changed.)

A.STOP

F.STOP

Adjust the brightness. 3

ND filter (p.26)

Adjust the 2 brightness.

Brightness control knob (p.26)

4.Return the microscope to bright-field microscopy.

Turn the
microscopy
1 selection knob.	DF
BF (bright-field) (p.33)

Adjust the 2 brightness.

Brightness control knob (p.26)

BF

FL2

FL1

The field and aperture diaphragms automatically return to what they were before the microscope was set to dark-field microscopy.

A.STOP

F.STOP

Adjust the brightness. 3

ND filter (p.26)

19

3 Differential Interference Contrast (DIC) Microscopy

When configured with the LV-UEPI

1.Mount objectives marked “LU”, universal quintuple nosepiece or motorized universal quintuple nosepiece, polarizer, analyzer, and DIC slider. (p.36, 38, 39, 49, and 56)

2.Focus on the sample with bright-field microscopy. (p.16)

3.Set the microscope for DIC microscopy.

Information:

The DIC slider can be operated to enable various microscopies, including sensitive DIC.

1 Push in.

Analyzer (p.38)

Push in and align 2 the marks.

Polarizer (p.36)

Crossed Nicols position.

3 Push in.

DIC slider (p.39)

Select the desired 4 magnification.

On the LV150A, use the nosepiece rotation buttons.

(p.35)

BF DF

A STOP F.STOP

Select “A” or “B”. 5

Rotate the inner knob. (p.39)

Match with
the objective	CF Plan
indication.	10X/ 0.30 A
	/0 BD DIC

Select the interference

color. 6

Rotate the top knob. (p.39)

Adjust the brightness. 7

ND filter (p.26)

Adjust the brightness. 8

Brightness control knob (p.26)

4.Return the microscope to the bright-field microscopy.

1Pull out.

Analyzer (p.38)

2Pull out.

Polarizer (p.36)

3Pull out.

DIC slider (p.39)

A STOP

F.STOP

BF

DF

Adjust the brightness. 5

ND filter (p.26)

Adjust the

4brightness.

Brightness control knob (p.26)

20

II. Microscopy

When configured with the LV-UEPI2

1.Mount objectives marked “LU”, universal quintuple nosepiece, polarizer or motorized quintuple nosepiece, polarizer, analyzer, and DIC slider. (p.36, 38, 39, 49, and 56)

2.Focus on the sample with bright-field microscopy. (p.17)

3.Set the microscope for DIC microscopy.

1Push in.

Analyzer (p.38)

Information:

The DIC slider can be operated to enable various microscopies, including sensitive DIC.

Push in and align 2 the marks.

Polarizer (p.36)

Crossed Nicols position.

3 Push in.

DIC slider (p.39)

Select the desired 4 magnification.

On the LV150A, use the nosepiece rotation buttons.

(p.35)

4.Return the microscope to bright-field microscopy.

1 Pull out.

Analyzer (p.38)

2 Pull out.

Polarizer (p.36)

3 Pull out.

DIC slider (p.39)

DF

A.STOP

F.STOP

BF

FL2

FL1

Select “A” or “B”. 5

Rotate the inner knob. (p.39)

Match with

the objective

CF Plan

indication.

10X/ 0.30 A

/0 BD DIC

Select the interference

color. 6

Rotate the top knob. (p.39)

Adjust the brightness. 7

ND filter (p.26)

Adjust the brightness. 8

Brightness control knob (p.26)

		A.STOP
		F.STOP
	BF	Adjust the
DF	FL2
	FL1	brightness. 5

ND filter (p.26)

Adjust the

4brightness.

Brightness control knob (p.26)

21