Leica TCS LSI User Manual
Leica TCS LSI
The World‘s First Super Zoom Confocal!
Technical Documentation
Scan Head
Motorized Zooms
Specifications
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Scanner |
Method |
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true confocal |
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Confocal channels |
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1 |
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Scanner |
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galvo (x,y) |
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Sequential scan |
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yes |
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Channel multiplexing |
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1 – 8 sequential |
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Resolution |
Range (min – max) |
[pixel] |
1282 – 20482 |
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Scan formats |
[pixel] |
128, 256, 512,1024, 2048 |
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Image depth |
[bit] |
8 or 12, switchable |
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Spectral Detection |
Spectral detection |
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yes |
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Type |
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continuously variable |
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Spectral resolution |
[nm] |
10 nm |
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Bandwidth |
[nm] |
430 – 750 |
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Detector |
Detector |
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1 |
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Detector type |
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ultra high dynamic PMT |
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Detector connection |
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direct |
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Confocal Zoom |
Zoom type |
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continuously variable |
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Zoom range |
[x] |
1x – 16x |
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Zoom increment |
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0.1 |
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Optical Zoom |
Zoom type |
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motorized/manual |
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Zoom range, Z16 APO/Z6 APO |
[x] |
0.57 – 9.2x/0.57 – 3.6x |
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Zoom increment |
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continuously / variable |
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Pinhole |
Pinhole type |
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motorized, variable |
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Range (min – max) |
[µm] |
35 – 600 |
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Pinhole adjustment |
[%] |
0 – 100 |
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Control |
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automated via GUI |
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Beam Splitter |
Type |
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high performance dichroics |
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Beam splitter wavelenght |
[nm] |
405/532; 488/635 |
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ND-Splitter for TLD |
[%] |
RT 30/70 |
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Beam splitter change |
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automated |
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Scan Modes |
2D: x-y, x-z, x-t |
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yes |
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3D: x-y-z, x-y-t, x-y-λ, x-z-y |
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yes |
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4D: x-y-z-t |
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yes |
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Speed |
Speed mode |
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uni/bi-directional |
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Line speed |
[Hz] |
800 |
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Line speed range |
[Hz] |
400, 600, 800 |
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Max@ 1282 |
[f/s] |
6.0 |
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standard@ 5122 |
[f/s] |
2.0 |
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Min@20482 |
[f/s] |
0.36 |
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Z16 APO A |
Type |
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Z16 APO A |
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Magnification range |
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0.57 – 9.2 |
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NA-range depending on magnification |
[nA] |
0.017 – 0.112 (with Obj. 1x) |
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Zoom positioning |
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continuously/UMC controlled |
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Fine focus optics |
yes |
motorized |
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Diaphragma |
yes |
motorized |
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Z6 APO A |
Type |
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Z6 APO A |
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Magnification range |
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0.57 – 3,6 |
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NA-range depending on magnification |
[nA] |
0.02 – 0.117 (with Obj. 1x) |
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Positioning |
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continuously UMC controlled |
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fine focus optics |
yes |
motorized |
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Diaphragma |
yes |
motorized |
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2
Supply Unit
Z-Drive
Microscope
Objectives
Software
Environment
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Laser |
Laser type |
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solid state |
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Number of lasers |
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max 4 |
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Laser options |
[nm] |
405, 488, 532, 635 |
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Excitation attenuation |
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AOTF |
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Excitation attenuation control |
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automated |
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Range |
[%] |
0 – 100 |
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Typ.power@Zoom 1, Obj 1x |
[mW] |
> 1 |
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Power depending on optical zoom |
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yes |
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Computer |
PC integration |
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yes |
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Processor |
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Intel Pentium M |
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HD-size |
[GB] |
120 |
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Operating system |
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XP-embedded |
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Interface |
Ethernet |
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1 |
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USB |
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4 |
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Fire wire |
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2 |
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Parallel |
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1 |
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Serial |
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1 |
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Mouse |
Type |
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optical |
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Monitor |
Monitor resolution |
[Pixel] |
1280x1024 |
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Monitor size |
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19“ |
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Power Supply |
Power supply integration |
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yes |
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Type |
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autoselect |
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Voltage range |
[V] |
100 – 240 |
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Power consumption |
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800 VA |
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Micro |
Z-focus |
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Galvanometer-stage |
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Z-stepsize |
[@500 m range] |
10 nm |
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Max Z-Range |
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1500 m |
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Types |
Microscope upright |
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Base CSQ |
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Focussing drive |
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motorized |
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Max travel range |
[mm] |
150 |
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Laser Safety Chamber |
Door angle, open |
[°] |
180 |
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Material |
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lasersafe acrylic glas |
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Inside height from ground plate |
[mm] |
180 |
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Depth min |
[mm] |
420 |
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Width inside |
[mm] |
555 |
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Heating unit connection |
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yes |
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Heating unit connection, diameter |
[mm] |
100 |
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Holder for micromanipulators |
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integrated |
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Transmitted light |
Intensity control |
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potentiometer |
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Macro-Objective |
Magnification |
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1x, 2x, 5x |
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NA |
[mm] |
variable |
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Working distance |
[mm] |
97/39/19 |
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Micro-Objectives ACS |
Magnification |
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10x, 40x, 63x |
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NA |
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0.3; 0.8; 1.15 |
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Working distance |
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0.3; 0.16; 0.15 |
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LAS AF LSI |
Basic aquisition license |
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system included |
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Life Data Mode |
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optional |
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Colocalisation |
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optional |
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Deconvolution |
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optional |
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3D-visualization |
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optional |
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Dye Finder |
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optional |
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Export formats |
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lif, tiff, avi, jpg |
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Ratings |
Humidity |
[%] |
10 – 80 |
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Operating temperature |
[°C] |
18 – 30 |
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Guaranteed stability |
[°C/h] |
23 +/- 2 |
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3
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