Leica ES2
Leica EZ4
Leica EZ4 HD
Manual
Congratulations!
You have just purchased a Leica E Series stereomicroscope - an excellent choice! The Leica E series is perfectly suited for practical instruction in schools and universities as well as for routine tasks in the laboratory and industrial production and testing. Viewing entire objects under magnification imparts perspectives and discoveries that would not be possible with the naked eye.
In developing our stereomicroscopes, we have placed great emphasis on simple, self-explana- tory operation. However, please take the time to read the instruction manual and the notes on operating safety, to learn about all the features and capabilities of your instrument so that you can use it safely and to its best advantage. Should you have any questions, please consult your local Leica representative. We are gladly at your service!
Leica E Series |
Manual |
2 |
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Chapter Overview
Safety and Use |
6 |
Leica ES2 |
10 |
Leica EZ4 and Leica EZ4 HD |
18 |
Eyepieces (only for Leica EZ4) |
29 |
Photography Using the Leica EZ4 HD |
37 |
The Camera Menu |
51 |
Care and Transport |
62 |
Specifications |
64 |
Dimensions |
67 |
Leica E Series |
Manual |
3 |
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Table of Contents
Leica ES2 |
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Overview: Leica ES2 |
11 |
Transport, Placement and Storage |
12 |
The Correct Interpupillary Distance |
13 |
Using the LED illuminator |
14 |
Focusing |
15 |
Magnification Display |
16 |
Adjusting the Resistance of the Focus Drive |
17 |
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Leica EZ4 and Leica EZ4 HD |
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Overview: Leica EZ4 |
19 |
Instrument variant: Leica EZ4 HD |
20 |
Transport, Placement and Storage |
21 |
The Correct Interpupillary Distance |
22 |
Switching on the LED Illuminator |
23 |
Controlling the Automatic Shutoff |
24 |
Illumination types |
25 |
Focusing |
26 |
Magnification Display |
27 |
Adjusting the Resistance of the Focus Drive |
28 |
Eyepieces (only for Leica EZ4) |
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Changing Eyepieces (Only for Leica EZ4, Open Version) |
30 |
Eyepieces and Eyecups (Only for Leica EZ4, Open Version) |
31 |
Dioptric Correction (Only for Leica EZ4, Open Version) |
32 |
Graticules (Only for Leica EZ4, Open Version) |
34 |
Graticules: Preparation |
35 |
Inserting the Graticules |
36 |
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Photography Using the Leica EZ4 HD |
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Leica EZ4 HD: Overview of the instrument |
38 |
General Instructions |
39 |
Introduction |
40 |
Standard delivery |
41 |
HD mode and PC mode |
42 |
Capturing Images without a Computer in HD Mode |
43 |
High-resolution Video Display without a Computer (HD mode) |
45 |
PC Mode: Use with a Computer |
46 |
PC Mode: Limitations |
47 |
PC Mode: Additional Use of a HDMI Device |
48 |
Configuring the White Balance |
49 |
Restoring Factory Settings |
50 |
Leica E Series |
Manual |
4 |
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Contents (Continued)
The Camera Menu |
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Optional Remote Control |
52 |
Viewing Images and Movies with the Optional Remote Control |
53 |
Calling up the Camera Menu |
54 |
COLOR (Automatic White Balance) |
55 |
COLOR (Manual White Balance) |
56 |
EXPOSURE |
57 |
RESOLUTION |
58 |
SETUP CAMERA (Camera Settings) |
59 |
SETUP USER (User-defined Settings) |
60 |
Predefined Illumination Scenarios |
61 |
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Care and Transport |
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Care and Transport |
63 |
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Specifications |
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Camera Module |
65 |
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Dimensions |
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Leica ES2 |
68 |
Leica EZ4 HD |
70 |
Leica EZ4, 10× Eyepieces |
72 |
Leica EZ4 without Eyepieces |
74 |
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Leica E Series |
Manual |
5 |
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Safety and Use
Leica E Series |
Manual |
6 |
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Safety Notes
Before installing, operating or using the instrument, it is mandatory to read this user manual. In particular, please observe all
safety instructions.
The "Safety Concept" brochure contains additional safety information regarding service work, requirements and the handling of the microscope, accessories as well as general safety instructions.
You can combine individual system articles with articles from external suppliers. Please read the user manual and the safety requirements of the supplier.
To maintain the unit in its original condition and to ensure safe operation, the user must follow the instructions and warnings contained in these user manuals.
Warning of a danger
This symbol indicates especially important information that is mandatory to read and observe. Failure to follow these inst-
ructions may pose hazards to personnel, cause instrument malfunctions or damage the instrument.
Hazardous electrical voltage
This symbol indicates especially important information that is mandatory to read and observe. Failure to follow these inst-
ructions may pose hazards to personnel, cause instrument malfunctions or damage the instrument.
Important information
This symbol indicates additional information or explanations that intend to
provide clarity.
EC Declaration of Conformity
Electrically operated accessories are constructed based on the state of the art of technology and are provided with an EC Declaration of Conformity.
Contact address
Leica Microsystems (Switzerland) Ltd. Industry Division
Max Schmidheiny Strasse 201 CH-9435 Heerbrugg (Switzerland)
Leica E Series |
Manual |
7 |
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Instructions for Use
Intended use
The E Series microscopes are optical instruments for improving the visibility of objects through magnification and illumination. It is used for observing and documenting.
Place of use |
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Use |
the |
instrument |
in |
enclosed, |
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dust-free |
rooms |
at |
+10 |
°C |
to |
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+40 °C only. Protect it from oil, chemicals and extreme humidity. It must be at least 10 cm from the wall and away from flammable substances.
Interference
Avoid large temperature fluctuations, direct sunlight and vibrations. These conditions can distort measurements and micrographic images.
In warm and warm-damp climatic zones, 
the individual components require special care in order to prevent the build-up of
fungus.
Use in clean rooms
Leica E Series microscopes can be used in clean rooms without any problems.
Handling electrical components
Never install any other plug (NEMA 5-15P) or unscrew any mechanical components unless expressly instructed to do
so in the instructions.
The microscope must be connected to a grounded outlet.
Always position the microscope so that you can disconnect it from the power supply at any time. The power cable is provided
as the power disconnect device.
Leica E Series |
Manual |
8 |
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Instructions for Use (Continued)
Intervention from the user
The microscopes of the E Series contain 
no components that can be maintained or repaired by the user. Exceptions are menti-
oned specifically in this manual.
Unless otherwise specified in this manual, repair and service work may be carried out by authorized Leica technicians
only.
Unauthorized alterations to the instrument or noncompliant use shall void all
rights to any warranty claims.
Maintenance
Leica E Series microscopes are basically
maintenance-free. To ensure that it always operates safely and reliably, we recommend that you take the precaution of contac-
ting the responsible service organization. Here you can arrange for periodic inspections and conclude a maintenance contract (recommended).
Accessories
Only those accessories may be used that are listed in this user manual or for which safe use has been confirmed by Leica Microsystems.
Danger of infection
Direct contact with eyepieces is a potential transmission method for bacterial and viral infections of the eye. The risk can be
kept to a minimum by using personal eyepieces for each individual or detachable eyecups.
Leica E Series |
Manual |
9 |
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Leica ES2
Leica E Series |
Manual |
10 |
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Overview: Leica ES2
1
2
3
4
5
6
7
110× fixed eyepieces for eyeglass wearers
2Carrying handle
3Two-level magnification changer 10× and 30×
4Focusing drive
5Integrated LED incident light illumination
6Integrated LED transmitted illumination
7Switch for incident and transmitted light
Leica E Series |
Manual |
11 |
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Transport, Placement and Storage
Built-in carrying device
All Leica E Series microscopes are equipped with a carrying handle for conveni-
ent and safe transport.
Setting up the microscope
1.Place the microscope on a flat tabletop.
2.Connect the power supply to a grounded socket.
Packing the device away
1.After use, wind up the cable as shown in the illustration.
2.Store the instrument so that it is protected against dust.
Leica E Series |
Manual |
12 |
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The Correct Interpupillary Distance
The Ideal Image |
Note for eyeglass wearers |
Setting the eye distance |
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The interpupillary distance is correctly |
If you wear glasses, fold the eyecups back |
1. |
Look into the eyepieces. |
set if you see a single circular image field |
– otherwise, fold the eyecups forwards. |
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when looking at a specimen. |
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2. |
Hold the eyepieces with both hands. Push |
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the eyepieces together or separate them |
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until you see a circular image. |
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You may need a short time to become familiar with using the instrument. Not to worry—after a little while, it will become automatic.
Leica E Series |
Manual |
13 |
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Using the LED illuminator
Iluminating the specimen
1.Position a specimen in the middle of the baseplate.
2.Switch the two LED illuminators on or off as desired.
After switching off, the LEDs remain lit for about another 4 seconds.
Tips on use
ϘϘ Use incident light for plastic, opaque objects.
ϘϘ Use transmitted light for transmitted light specimens or transparent objects.
ϘϘ Use the combination of both LED illuminators for partially transparent objects.
Leica E Series |
Manual |
14 |
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Focusing
Focusing raises and lowers the stereomi- 
croscope using the focusing drive. The specimen is brought into sharp focus as soon as
it is in the focal point of the objective.
The focusing drive can be operated either leftor right-handed.
Focusing
1.Set the magnification changer to the lowest level (10).
2. Set the working distance to approx.
100 mm for coarse focusing.
3.Set the magnification changer to the second level (30).
4.Use the focusing drive once again for fine focus.
After you have adjusted the image sharp- 
ness at high magnification, it is maintained even if you switch to a lower magnification
(parfocal).
Focusing drive torque
Leica E Series |
Manual |
15 |
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Magnification Display
You can read the configured magnifica- 
tion on the right-hand rotary knob of the Leica ES2. The additional magnification provi-
ded by the eyepieces is already incorporated in the scale.
Leica E Series |
Manual |
16 |
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Adjusting the Resistance of the Focus Drive
Adjusting the resistance
Is the focusing drive too easy or too difficult to adjust? No problem - the resistance can be adjusted individually depending on your personal preferences:
1.Grip the drive knobs with both hands and turn them towards each other until the desired resistance is reached during focusing.
Leica E Series |
Manual |
17 |
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Leica EZ4 and Leica EZ4 HD
Leica E Series |
Manual |
18 |
|
|
|
Overview: Leica EZ4
1
2
3
4
5
6
7
110x fixed eyepieces for eyeglass wearers
2Carrying handle
3Magnification changer
4Focusing drive
5Integrated LED incident light illumination
6Illumination control
7Integrated LED transmitted illumination
Leica E Series |
Manual |
19 |
|
|
|
Instrument variant: Leica EZ4 HD
1
2
3
4
5
6
7
8
9
110x fixed eyepieces for eyeglass wearers
2Integrated digital camera with SD card slot
3Carrying handle
4Magnification changer
5IR receiver for optional remote control
6Focusing drive
7Integrated LED incident light illumination
8Illumination control
9Integrated LED transmitted illumination
Leica E Series |
Manual |
20 |
|
|
|
Transport, Placement and Storage
Built-in carrying device
All Leica E Series microscopes are equipped with a holder for convenient and
safe transport.
Setting up the microscope
1.Place the microscope on a flat tabletop.
2.Connect the power supply to a grounded socket.
Packing the device away
1.After use, wind up the cable as shown in the illustration.
2.Store the instrument so that it is protected against dust.
Leica E Series |
Manual |
21 |
|
|
|
The Correct Interpupillary Distance
The Ideal Image |
Note for eyeglass wearers |
Setting the eye distance |
|
The interpupillary distance is correctly |
If you wear glasses, fold the eyecups back |
1. |
Look into the eyepieces. |
set if you see a single circular image field |
– otherwise, fold the eyecups forwards. |
|
|
when looking at a specimen. |
|
2. |
Hold the eyepieces with both hands. Push |
|
|
|
the eyepieces together or separate them |
|
|
|
until you see a circular image. |
|
|
You may need a short time to become familiar with using the instrument. Not to worry—after a little while, it will become automatic.
Leica E Series |
Manual |
22 |
|
|
|
Switching on the LED Illuminator
Automatic shutoff
If the illumination is not operated for 60 minutes, it switches off automatically.
The illumination can be reactivated by pressing a button on the membrane keypad.
2.Switch the two LED illuminators on or off as desired:
LED illumination
1.Position a specimen in the middle of the baseplate.
ϘϘ
ϘϘ
ϘϘ
Use incident light for plastic, opaque objects
Use transmitted light for transmitted light specimens or transparent objects.
Use the combination of both LED illuminators for partially transparent objects.
For a detailed description of the illuminator, refer to page 25.
Leica E Series |
Manual |
23 |
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