The Leica
Darkfield
Quebec
Colony Counter Model
3325/3326/3327/3328
LEICA DARKFIELD QUEBEC® Colony Counter
Model 3325/3326/3327/3328
Instruction Manual
1.0 INTRODUCTION
The LEICA DARKFIELD QUEBEC® Colony Counter provides efficient, accurate and convenient colony counting. Even, glarefree illumination over the entire culture plate assures clear, quick results. By touching the colony with the counting probe (Models 3327/3328), colonies are recorded on the digital display; they appear bright and are readily distinguished from other structures. Wolffheugel and Stewart counting plates are available.
2.0 UNPACKING & ASSEMBLY
Carefully remove the LEICA DARKFIELD QUEBEC® Colony Counter and accessories from the packing box and inspect them to assure damage did not occur in shipping.
Place the instrument on a hard, flat surface.
A leg located on the bottom can be set at either a tilt angle or locked flat to the instrument base.
Similarly, the 1.5X magnification lens may be raised or lowered for user preference. A 1.5X auxiliary lens (Catalog No. 3331) is available as an accessory and is designed to be placed over the existing lens to increase the total magnification to 3X.
The LEICA DARKFIELD QUEBEC® Colony Counter (Model 3327 & 3328) includes a five-digit L.C.D. counter with a reset button, a ground contact and counting probe (Figure 1). An optional push button counter also is available.
Explanation of Warning Symbols
CAUTION: Replace with same type and rating fuse
CAUTION: Risk of electric shock
!CAUTION: (Refer to accompanying documents)
The counting probe (Models 3327/3328 only) is shipped with the needle tip reversed for protection from damage. To assemble the counting probe for use, loosen the knurled nut, remove the needle, place the blunt end in the chuck and retighten.
The ground contact has a magnetic base and is attached to the instrument case so the arm extends over the culture plate and touches the surface of the agar.
The push button counter plugs into the two-hole receptacle at the rear of the colony counter.
3.0 TESTING THE INSTRUMENT
Before using the LEICA DARKFIELD QUEBEC® Colony Counter it should be tested. Plug the electrical cord into a grounded 120V/60 cycle AC outlet (Model 3325/3327) or
230V/50 cycle AC outlet (Model 3326/3328).
Turn the instrument on using the switch located on the rear panel. Touch the tip of the counting probe to the ground arm probe. A count should register on the L.C.D. display, if the colony counter is operational.
To return the L.C.D. display counter to “0” depress the reset button.
4.0 CARE OF THE COUNTING PROBE
Care should be taken to keep the tip of the counting probe sharp. If the needle is blunt, small colonies will be difficult to count and accurately register.
The probe tips touching media and/or colonies should be cleaned as dictated by good laboratory procedures. Solutions of 70% isopropyl alcohol, iodine/alcohol, Lysol® Brand or formaldehyde may be used to clean the tip without harm.
Figure 1 |
Figure 2 |
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