Page 1
5
Draft Labeling
Subject to Technical Review
Select to finish programming the 96 well microplate.
6
Select to run the 96 well microplate queue.
7
Plate results list.
Run Samples
Add 96 Well Plate Samples to the Queue
4
8
Plate sample results.
PN C13232AA7 FINAL
4-7
Page 2
Run Samples
Draft Labeling
Subject to Technical Review
Run Results
Run Results
Green Screens need to be replaced throughout
• Camera image results.
• Graphical results.
• Carousel cell type, count and viability results.
4-8
PN C13232AA7 FINAL
Page 3
• Plate cell type, count and viability results.
Draft Labeling
Subject to Technical Review
Camera Image Options
• Grey level histogram, Image fit to window.
Run Samples
Run Results
4
• Grey level histogram, Image actual size.
PN C13232AA7 FINAL
4-9
Page 4
Run Samples
Draft Labeling
Subject to Technical Review
Run Results
• Annotated image, Image fit to window.
• Binary image, Image fit to window.
• Annotated and Binary image, Image fit to windows.
4-10
PN C13232AA7 FINAL
Page 5
• Grey level histogram.
Draft Labeling
Subject to Technical Review
Run Samples
Run Results
4
PN C13232AA7 FINAL
4-11
Page 6
Run Samples
Draft Labeling
Subject to Technical Review
Bioprocess Results
Bioprocess Results
Green Screens need to be replaced throughout
What Is A Bioprocess?
A bioprocess consists of the analysis results of a number of samples taken from the same source
over a period of time. The source might be a bioreactor, a fermentation vessel or even a laboratory
flask. A source may be associated with many bioprocesses as when one bioprocess finishes (for
example when a bioreactor is harvested) then another separate bioprocess can be started. The
measurements associated with a single bioprocess can be manipulated (opened and closed) as if
they were a single entity. The Vi-CELL BLU is designed to make it easy to monitor multiple,
asynchronous, bioprocesses at the same time. Changes in selected analysis parameters over time
can be displayed making it easy for example to check the progress of a bioreactor and to choose the
optimum time for harvest.
The Vi-CELL BLU bioprocess feature allows convenient, automated “tracking” of any of the
measured cell culture parameters and calculates growth rate, doubling time, all essential to
optimum bioreactor productivity. Data points are recorded and stored, eliminating the necessity
for manual recording of the cell culture measurements. See.
Growth Rate and Doubling times are calculated from the results of the last two runs using the
formulas:Colin asks if Vi-Cell BLU does this
Growth rate per hour = ( ln V2 - ln V1 ) / ( t2 - t1 )
Doubling time in hours= ln 2 / Growth rate per hour
Where
V1 = Viable cell concentration in cells/mL at elapsed time t1 in hours
V2 = Viable cell concentration in cells/mL at elapsed time t2 in hours
• Bioprocess list results.
4-12
PN C13232AA7 FINAL
Page 7
• Add Bioprocess screen.
Draft Labeling
Subject to Technical Review
• Bioprocess Total Cells/mL results.
Run Samples
Bioprocess Results
4
• Bioprocess camera results.
PN C13232AA7 FINAL
4-13
Page 8
Run Samples
Draft Labeling
Subject to Technical Review
Signoff Results
• Bioprocess size distribution results.
Signoff Results
Green Screens need to be replaced throughout
1
When Security is enabled, you need to select to signoff results after sample processing.
2
Enter your User Name and Password and select Creation (CRE) Review (REV) or Approve (APR)..
4-14
PN C13232AA7 FINAL
Page 9
3
Draft Labeling
Subject to Technical Review
Your approval credentials are shown on the Run Result screen.
Run Samples
Signoff Results
4
PN C13232AA7 FINAL
4-15
Page 10
Run Samples
Draft Labeling
Subject to Technical Review
Signoff Results
4-16
PN C13232AA7 FINAL
Page 11
Reviewing/Reanalyze Data
Draft Labeling
Subject to Technical Review
1
Select and to choose a file to review.
2
Select a sample to review or reanalyze and select .
3
If desired, change the Cell Type,.
CHAPTER 5
Data Analysis
PN C13232AA7 FINAL
5-1
Loading...