Beckman Coulter Optima XPN User Manual

User Guide

Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

Optima XPN Series

Preparative Ultracentrifuges

PN B08115AF
February 2018

Optima XPN
Ultra Centrifuge
User Guide

PN B08115AF (February 2018)

© 2018 Beckman Coulter, Inc.
All rights reserved

Trademarks

Beckman Coulter, the stylized logo, and the Beckman
Coulter product and service marks mentioned herein are
trademarks or registered trademarks of Beckman Coulter,
Inc. in the United States and other countries.

All other trademarks, service marks, products, or services
are trademarks or registered trademarks of their
respective holders.

Contact Us

If you have any questions, contact our Customer Support
Center.

• Worldwide, find us via our website at

www.beckmancoulter.com/customersupport/support.

• In the USA and Canada, call us at 1-800-369-0333.

• Outside of the USA and Canada, contact your local

Beckman Coulter Representative.

Printed in U.S.A.

Revision History

B08115AA, Initial Issue, 09/2011

Issue Version AB, 01/2014

Manual updated per Canadian Standards Association (CSA) guidelines.

Issue Version AC, 01/2015

Changes or additions were made to: Getting Access to the Sample; Restoring the Instrument to
Operating Condition.

Issue Version AD, 04/2016

Changes or additions were made to: Multiple Compliance

Issue Version AE, 09/2016

Changes or additions were made to:

• Chemical and Biological Safety

• CHAPTER 2, System References

• CHAPTER 8, References Page

Issue Version AF, 02/2018

Changes or additions were made to: Table C.1, Diagnostics/User Messages Chart.

Note: Changes that are part of the most recent revision are indicated in text by a bar in the margin
of the amended page.

This document applies to the latest software listed and higher versions. When a subsequent software version affects the
information in this document, a new issue will be released to the Beckman Coulter website. For labeling updates, go to

www.beckmancoulter.com and download the latest version of the manual or system help for your instrument.

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Revision History

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Safety and Notices

This chapter presents the important notices that apply to the instrument and describes the
conventions used in the document.

Alerts for Danger, Warning, Caution, Important, and Note

DANGER

DANGER indicates an imminently hazardous situation which, if not avoided, will
result in death or serious injury. This signal word is to be limited to the most
extreme situations.

WARNING

WARNING indicates a potentially hazardous situation which, if not avoided, could
result in death or serious injury. May be used to indicate the possibility of
erroneous data that could result in an incorrect diagnosis (does not apply to all
products).

CAUTION

CAUTION indicates a potentially hazardous situation, which, if not avoided, may
result in minor or moderate injury. It may also be used to alert against unsafe
practices. May be used to indicate the possibility of erroneous data that could
result in an incorrect diagnosis (does not apply to all products).

IMPORTANT

Following the advice in the Important adds benefit to the performance of a piece of equipment or to a
process.

NOTE NOTE is used to call attention to notable information that should be followed during installation, use,

or servicing of this equipment.

IMPORTANT is used for comments that add value to the step or procedure being performed.

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Safety and Notices

Safety Notices

Safety Notices

Before installing, using, or maintaining the instrument, be certain that you know all the following
precautions.

Information

CAUTION

Read all product manuals and consult with Beckman Coulter-trained personnel
before attempting to operate instrument. Do not attempt to perform any
procedure before carefully reading all instructions. Always follow product labeling
and manufacturer’s recommendations. If in doubt as to how to proceed in any
situation, contact your Beckman Coulter Representative.

Installation

CAUTION

This instrument is designed to be installed by a Beckman Coulter Field Service
representative. Installation by anyone other than authorized Beckman Coulter
personnel invalidates any warranty covering the instrument. Also, should the
instrument need to be moved, a Beckman Coulter Field Service representative
must reinstall and re-level the instrument in its new location.

WARNING

Do not place the ultracentrifuge near areas containing flammable or combustible
fluids, or any other source of vapors that could enter the ultracentrifuge air
system and be ignited by the motor.

WARNING

Ultracentrifuge operations generate high energy levels that require precautions
against sudden movements that could result from the rare event of a rotor failure.
Maintain a 30-cm. (1-ft.) clearance envelope around and above the
ultracentrifuge. Do not install any equipment or furniture in this envelope. While
the ultracentrifuge is running, keep the envelope clear of any persons or objects
and do not reach into the envelope except when required to change operating
controls.

vi

PN B08115AF

Replacement Parts

WARNING

Do not replace any centrifuge components with parts not specified for use on this
instrument.

Service

WARNING

Any servicing of this equipment that requires removal of any covers can expose
parts which involve the risk of electric shock or personal injury. Make sure that the
power switch is turned off, and the instrument is disconnected from the main
power source, by removing its power plug from the receptacle. Refer such
servicing to qualified personnel.

Safety and Notices

Safety Notices

NOTE It is your responsibility to decontaminate the instrument and accessories before

requesting service by Beckman Coulter Field Service.

Power Supply

DANGER

To reduce the risk of electrical shock, this instrument uses a three-wire electrical
cord and plug to connect this equipment to earth-ground. Make sure that the
matching wall outlet receptacle is properly wired and earth-grounded.

Fuse Replacement

WARNING

Fuses protect certain electrical circuits within this instrument against overcurrent
conditions. The fuse is not customer replaceable. For continued protections,

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vii

Safety and Notices

Safety Notices

please contact Beckman Coulter Field Service.

Mechanical Safety

DANGER

For safe operation of the equipment, observe the following:

• Use only the Beckman Coulter rotors and accessories designed for use in this

• Do not exceed the maximum rated speed of the rotor in use.

• NEVER attempt to slow or stop a rotor by hand.

• Do not move the centrifuge while the drive motor is spinning.

• In the event of a power failure, do not attempt to retrieve the sample from the

instrument.

instrument for at least one hour. Then follow the instructions for recovery of
the sample in the Maintenance and Troubleshooting chapter.

Chemical and Biological Safety

DANGER

Normal operation may involve the use of solutions and test samples that are
pathogenic, toxic, or radioactive. Such materials require that you take all
necessary safety precautions.

• Handle body fluids with care because they can transmit disease. No known
test offers complete assurance that they are free of micro-organisms.

• Handle all infectious samples according to good laboratory procedures and
methods to prevent spread of disease.

• Because spills may generate aerosols, observe proper safety precautions for
aerosol containment.

• Some of the most virulent infectious agents —Hepatitis (B and C) and HIV (I-V)
viruses, atypical mycobacteria, and certain systemic fungi—require
additional emphasis on aerosol protection.

• Do not run toxic, pathogenic, or radioactive materials in a rotor without taking
appropriate safety precautions.

• Risk Group II materials (as identified in the World Health Organization
Laboratory Biosafety Manual) require biosafe containment. Materials of a
higher group require more than one level of protection.

viii

• Dispose of all waste solutions according to appropriate environmental health
and safety guidelines.

• Do not centrifuge flammable or explosive vapors, or materials capable of
hazardous chemical reactions.

PN B08115AF

WARNING

California Proposition 65:

This product may contain chemicals known to the State of California to cause
cancer and birth defects or other reproductive harm.

Precautions with Liquids

WARNING

Do not place containers holding liquid on or near the chamber door. Liquid, if
spilled, may get into the instrument and damage electrical or mechanical
components.

Volatile Liquids

Safety and Notices

Certification

DANGER

This instrument is not designed for use with materials capable of developing
flammable or explosive vapors, or hazardous chemical reactions. Do not
centrifuge such materials (for example, chloroform or ethyl alcohol) in this
instrument nor handle or store them near the centrifuge.

Certification

To ensure full system quality, Beckman Coulter Optima XPNs have been manufactured in a
registered ISO 9001 or 13485 facility. They have been designed and tested to conform to (when used
with Beckman Coulter rotors) the laboratory equipment requirements of applicable regulatory
agencies. Declarations of conformity and certificates of compliance are available at
www.beckmancoulter.com.

Scope of Manual

This manual is designed to familiarize you with the Optima XPN, its functions, specifications,
operation, and routine operator care and maintenance. Read this entire manual, especially the
safety notices and all safety-related information, before operating the instrument or performing
maintenance.

PN B08115AF

NOTE

If the instrument is used in a manner other than specified in this manual, the safety and performance
of this equipment could be impaired. Further, the use of any equipment other than that recommended
by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically
recommended in this manual and/or the appropriate rotor manual is the sole responsibility of the user.

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Safety and Notices

CFC-Free Centrifugation

CFC-Free Centrifugation

To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of the
Optima XPN ultracentrifuge.

Software Copyright

The software and other information incorporated into the Optima XPN is protected by international
copyright laws. Unauthorized copying, use, distribution, transfer, or sale is a violation of those laws
that may result in civil or criminal penalties. This computer program is also subject to additional
restrictions contained in the following Microsoft OEM Customer License Agreement for Embedded
Systems:

“If you use the Device to access or utilize the services or functionality of Microsoft Windows XP
Server (all editions), or use the Device to permit workstation or computing devices to access or
utilize the services or functionality of Microsoft Windows XP Server, you may be required to obtain
a Client Access License for the Device and/or each such workstation or computing device. Refer to
the End-User License Agreement for Microsoft Windows XP Server for additional information.” The

End-User License Agreement is available on the Microsoft Embedded Systems website.

x

PN B08115AF

RoHS Notice

These labels and materials declaration table (the Table of Hazardous Substance's Name and
Concentration) are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006
“Marking for Control of Pollution Caused by Electronic Information Products” requirements.

China RoHS Caution Label

This label indicates that the electronic information product contains certain toxic or hazardous
substances. The center number is the Environmentally Friendly Use Period (EFUP) date, and
indicates the number of calendar years the product can be in operation. Upon the expiration of the
EFUP, the product must be immediately recycled. The circling arrows indicate the product is
recyclable. The date code on the label or product indicates the date of manufacture.

Safety and Notices

RoHS Notice

China RoHS Environmental Label

This label indicates that the electronic information product does not contain any toxic or hazardous
substances. The center “e” indicates the product is environmentally safe and does not have an
Environmentally Friendly Use Period (EFUP) date. Therefore, it can safely be used indefinitely. The
circling arrows indicate the product is recyclable. The date code on the label or product indicates
the date of manufacture.

PN B08115AF

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Safety and Notices

!

Summary of Instrument Labels

Summary of Instrument Labels

This section provides information for some labels and symbols appearing on the Optima XE
instrument housing. These labels and symbols may be associated with user-serviceable procedures.
Individual hazards associated with a specific procedure in this manual may use these labels and
symbols, and are included in

Caution Symbol

This symbol indicates a caution message and appears adjacent to an explanation or other symbols
that define the caution.

Warnings or Cautions within the procedures for that task.

Universal Serial Bus (USB)

This symbol indicates the location of a universal serial bus (USB) connector.

Ethernet

This symbol indicates the location of an ethernet connector.

xii

PN B08115AF

Recycling Label

A28219-AA

This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE)
Directive of the European Union. The presence of this marking on the product indicates:

• the device was put on the European Market after August 13, 2005 and

• the device is not to be disposed of via the municipal waste collection system of any member

state of the European Union.

It is very important that customers understand and follow all laws regarding the proper
decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing
this label, please contact your dealer or local Beckman Coulter office for details on the take-back
program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal
of the device.

Safety and Notices

Summary of Instrument Labels

Multiple Compliance

This symbol indicates compliance with:

• 169502 – This label indicates recognition by a Nationally Recognized Testing Laboratory (NRTL)
that the instrument has met the relevant product safety standards.

• The RCM mark is intended for use on products that comply with Australian communications
Media Authority (ACMA) EMC Requirements.

• Recycling – Refer to the Recycling Label section in this document.

• – A “CE” mark indicates that a product has been assessed before being placed on the

market, and has been found to meet European Union safety, health, and/or environmental
protection requirements.

PN B08115AF

xiii

Safety and Notices

Summary of Instrument Labels

CAUTION Static Sensitive Area

Indicates an area of the instrument which is sensitive to static electrostatic discharge (ESD). To
prevent damage due to electrostatic discharge, always wear a properly earth-grounded wrist strap
while operating this instrument. For details on proper grounding, see IEEE standard P1100.

DANGER High Voltage

Operation, replacement or servicing of any components where contact with bare, live hazardous
parts could occur, possibly resulting in electric shock, should only be performed by your
Beckman Coulter representative.

Protective Ground

This symbol is used to indicate a protective ground. This instrument must be properly grounded.
Do not under any circumstances operate the instrument unless it is properly grounded.

Alternating Current

xiv

This symbol is used to indicate an alternating current (also known as “AC”).

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Output Plate Label

Safety and Notices

Summary of Instrument Labels

This is the label that indicates what the output rating are on the instrument.

Rotor Rotation

This indicates the direction of instrument rotor rotation.

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xv

Safety and Notices

Symbols

Symbols

xvi

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Contents

Revision History,iii

Safety and Notices,v

Alerts for Danger, Warning, Caution, Important, and Note,v

Safety Notices,vi

Information,vi
Installation,vi
Replacement Parts,vii
Service,vii
Power Supply,vii
Fuse Replacement,vii
Mechanical Safety, viii
Chemical and Biological Safety,viii
Precautions with Liquids,ix
Volatile Liquids,ix

Certification,ix

Scope of Manual,ix

CFC-Free Centrifugation,x

Software Copyright,x

RoHS Notice,xi

China RoHS Caution Label,xi
China RoHS Environmental Label,xi

Summary of Instrument Labels,xii

Caution Symbol,xii
Universal Serial Bus (USB),xii
Ethernet,xii
Recycling Label,xiii
Multiple Compliance,xiii
CAUTION Static Sensitive Area,xiv
DANGER High Voltage,xiv
Protective Ground,xiv
Alternating Current,xiv
Output Plate Label,xv
Rotor Rotation,xv

Symbols,xvi

CHAPTER 1: Description,1-1

Touch Screen,1-1

xvii

Contents

Name Rating Plate,1-1

Rotor Chamber,1-1

Vacuum System,1-1

Temperature Sensing and Control,1-2

Drive,1-2

Safety Features,1-2

Door,1-2
Barrier Ring,1-3
Imbalance Detector,1-3
Overspeed System,1-3
Dynamic Rotor Inertia Check (DRIC),1-3

Specifications,1-3

Control Features,1-4
Physical Data,1-6
Audible Sounds,1-7

Available Rotors,1-8

CHAPTER 2: The Touch Screen Interface,2-1

Areas on the Screen,2-1

Header Bar,2-1

Home Page Button,2-2
Menu Button,2-2
Status Display,2-2
Help Button,2-3

Footer Bar,2-3

Start Button,2-4
Stop Button,2-4
Footer Bar on the Home Page,2-5
Footer Bar on Other Pages,2-5
Vacuum Display/Button,2-5
System Name,2-5
Accel and Decel Display/Button,2-6

Page Display Area,2-6

Help Messages,2-6

Item Help,2-6
Global Help,2-7

xviii

System References,2-8

About Your Instrument,2-9

CHAPTER 3: Operations,3-1

Manual Operation,3-1

Step 1: Start on the Home Page,3-2
Step 2: Set the Speed and Rotor,3-3
Step 3: Set the Acceleration and Deceleration Profiles,3-5

Step 4: Set the Time,3-6
Step 5: Set the Temperature,3-7
Step 6: Start the Run,3-9

Zonal and Continuous Flow Operation,3-10

Preparing for the Run,3-11
Starting the Run,3-11
Loading the Sample,3-12
Running the Sample,3-12
Unloading the Sample,3-12
Stopping the Run,3-13
Finishing the Run,3-13

CHAPTER 4: Configuration,4-1

Managing Your Network ,4-1

Setting Up the Network ,4-1
Selecting a Printer,4-2
Setting Up Email ,4-3
Setting Up VNC,4-4
Enabling API,4-4

Contents

Managing Users,4-5

Adding Users,4-5
Requiring Login,4-6
PIN Expiration and Logout Timer,4-7
User Options,4-8

Managing Rotors,4-8

Adding Rotors,4-9
Requiring Rotor Selection,4-9

Managing Reports,4-10

User Access,4-10
Filter Data,4-10
Graph Data,4-12
Print Data,4-13
Export Data,4-14
Auto Print and Auto Export Run History Data,4-16

To enable Auto Print: ,4-16
To enable Auto Export:,4-16
Run Comments,4-17
E-Signature,4-18

CHAPTER 5: Programs,5-1

Creating Programs,5-1

Running Programs,5-5

Editing Programs,5-6

Deleting Programs,5-6

xix

Contents

CHAPTER 6: Calculations ,6-1

Using Calculations,6-1

Calculations Page,6-1
Reduce Rotor Speed For Dense Solutions,6-3
Reduce Rotor Speed For Precipitating Solutions,6-4
Sedimentation Coefficient From Run Data,6-5
Sedimentation Coefficient From Molecular Mass ,6-7
Pelleting Time,6-8
Refractive Index ,6-9
Concentration Measures ,6-10

CHAPTER 7: Simulations ,7-1

Using Simulations,7-1

Simulations Page,7-1
ESP RNA Pelleting Best Run,7-3
ESP RNA Pelleting Fast Run,7-5
ESP Pelleting Run,7-6
ESP Rate Zonal Run,7-8
ESP Plasmid Run,7-9
Substitute Rotor Run,7-11

CHAPTER 8: Functional Pages,8-1

Home Page,8-3

Home Page (Run in Progress),8-5

Set Speed Page,8-6

Select Rotor and Labware Page (Library),8-7

Set Time Page,8-8

Delay Start Page,8-9

Set Speed ω²t Time Page,8-10

Set Temperature Page,8-11

Set Acceleration/Deceleration Profiles Page,8-12

Example Acceleration and Deceleration Profiles,8-13

Login Page,8-14

Select Program Page,8-15

New/Edit/View Program Page,8-16

Select Rotor and Labware Page (Catalog),8-18

New/Edit Step Page,8-19

xx

Program Log Page,8-20

Export Page,8-21

Authorize Users Page,8-22

Import Page,8-23

Real-Time Run Data/Historical Run Data Page,8-24

Run Graph Options Page,8-25

Run History Page,8-26

Run History Filter Page,8-28

Before Run/After Run Comment Page,8-29

Menu Page,8-30

System Options Page,8-31

The Basic Tab,8-31
The System Tab,8-32
The Network Tab,8-33
The Users Tab,8-34
The Reports Tab,8-35

Select Language Page,8-36

Contents

User Options Page,8-37

Reset User PIN Page,8-38

Select Image Page,8-39

Set Date and Time Page,8-40

System Log Page,8-41

Manage Rotors Page,8-42

Add to Rotor Library Page,8-43

Diagnostic History Page,8-44

Set Sound Page,8-45

Custom Sounds Page,8-46

Archive Data Page,8-47

Setup Network Page,8-48

Select Printer Page,8-49

Setup Email Page,8-50

Setup VNC Page,8-51

Manage Users Page,8-52

Add/Edit User Page,8-53

Authorize Programs Page,8-54

References Page,8-55

Rotor Catalog Page,8-56

Compatible Tubes for Rotor Page,8-57

Labware Catalog Page,8-58

xxi

Contents

Chemical Resistances Page,8-59

Calculations Page ,8-60

Reduce Rotor Speed for Dense Solutions Page,8-61

Reduce Rotor Speed for Precipitating Solutions Page,8-62

Determine Sedimentation Coefficient from Run Data Page,8-63

Determine Sedimentation Coefficient from Molecular Mass Page,8-64

Calculate Pelleting Time Page,8-65

Calculate Concentration Measures Page,8-66

Calculate Refractive Index Page,8-67

About Page,8-68

Zonal/Continuous Flow Authorization Page,8-69

Zonal/Continuous Flow Operation Page,8-70

Simulations Page ,8-72

ESP RNA Pelleting in CsCl with GuSCN, Optimized for Purity Page,8-73

Select Rotor and Labware Page (Catalog/Library),8-74

ESP RNA Pelleting in CsCl with GuSCN, Optimized for Speed Page,8-75

ESP Pelleting Separation Page,8-76

ESP Optimized Plasmid DNA Separation Page,8-78

ESP Rate Zonal Separation Page,8-79

Substitute Rotor Run Page,8-80

CHAPTER 9: Maintenance and Troubleshooting,9-1

Field Service,9-1

Rotors and Labware,9-1

Cleaning,9-1

Instrument Surfaces,9-1
Rotor Chamber,9-2
Chamber Door O-ring,9-2

Decontamination,9-2

Sterilization and Disinfection,9-2

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Diagnostics/User Messages,9-3

Retrieving Your Sample in Case of Power Failure,9-3

During a Run,9-3

Rotor Spinning at Restoration,9-3

Rotor Stopped at Restoration,9-3
Getting Access to the Sample,9-4
Restoring the Instrument to Operating Condition,9-9

Storage and Transportation,9-10

Supply List,9-11

Replacement Parts,9-11

Supplies,9-11

APPENDIX A: Preinstallation Requirements,A-1

Overview,A-1

Space Requirements,A-1

Safety,A-1

Ventilation,A-2

Temperature,A-2

Electrical Requirements,A-2

APPENDIX B: Special Warranty for the Optima XPN,B-1

Special Warranty,B-1

Contents

APPENDIX C: Diagnostics,C-1

Overview,C-1

Diagnostics/User Messages Chart,C-1

APPENDIX D: Third Party Contributions and Legal Notices,D-1

xxiii

Illustrations

Illustrations

2.1 Home Page,2-1

2.2 Header Bar,2-1

2.3 Home Page Button,2-2

2.4 Menu Button,2-2

2.5 Ready Status,2-2

2.6 Header Bar - Ready Status,2-3

2.7 Header Bar - Running Status,2-3

2.8 Header Bar - Stopping Status,2-3

2.9 Header Bar - Warning Message,2-3

2.10 Header Bar - Error Message,2-3

2.11 Help Button,2-3

2.12 Start Button,2-4

2.13 Stop Button,2-4

2.14 Footer Bar - Home Page,2-5

2.15 Footer Bar on Other Pages,2-5

2.16 Item Help Button,2-6

2.17 Global Help Button attached to Help Button,2-7

2.18 Home Page Help with ghost image,2-7

2.19 Transparency Button,2-7

2.20 Home Page Help with opaque screen,2-8

2.21 Help Navigation Buttons,2-8

2.22 References Page,2-9

2.23 About Page,2-10

3.1 Home Page,3-2

3.2 Zonal Operation Page with Cancel Button,3-3

3.3 Set Speed Page,3-3

3.4 Select Rotor and Labware Page,3-4

3.5 Set Acceleration/Deceleration Profiles Page,3-5

3.6 Set Time Page,3-6

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3.7 Delay Start Page,3-7

3.8 Set Temperature Page,3-8

3.9 Start Button,3-9

3.10 Zonal Page,3-10

3.11 Continuous Flow Page,3-12

4.1 Run History Button,4-10

4.2 Run History Filter,4-11

4.3 Set From Date and Time,4-11

4.4 Run History,4-12

4.5 Run Graph Button,4-12

4.6 Run History Button,4-13

4.7 Run Graph Options,4-13

4.8 Run Graph Button,4-14

4.9 Run History Button,4-14

4.10 Run History Button,4-15

4.11 Run History,4-15

4.12 Run History Button,4-15

4.13 Before Run Comment,4-18

Illustrations

4.14 Signature,4-19

5.1 Program Button,5-1

5.2 Home,5-1

5.3 Select Program,5-2

5.4 Unnamed program,5-2

5.5 New Program Name,5-3

5.6 Set Acceleration/Deceleration Profiles,5-3

5.7 Select Rotor and Labware,5-4

5.8 New Step,5-4

5.9 New Step in a numbered sequence,5-5

5.10 Program Button,5-5

5.11 Program Button,5-6

5.12 Program Button,5-6

6.1 Menu Page,6-2

6.2 Calculations Page,6-3

6.3 Reduce Rotor Speed for Dense Solutions Page,6-4

6.4 Reduce Rotor Speed for Precipitating Solutions Page,6-5

6.5 Determine Sedimentation Coefficient From Run Data Page,6-6

6.6 Determine Sedimentation Coefficient from Molecular Mass
Page,6-7

6.7 Pelleting Time Page,6-8

6.8 Calculate Refractive Index Page,6-10

6.9 Calculate Concentration Measures Page,6-11

7.1 Menu Page,7-2

xxv

Illustrations

7.2 Simulations Page,7-3

7.3 ESP RNA Pelleting in CsCI with GuSCN, Optimized for Purity
Page,7-4

7.4 ESP RNA Pelleting in CsCI with GuSCN, Optimized for Speed
Page,7-5

7.5 ESP Pelleting Separation Page,7-7

7.6 ESP Rate Zonal Separation Page,7-8

7.7 ESP Optimized Plasmid DNA Separation Page,7-10

7.8 Substitute Rotor Run Page,7-11

8.1 Home Page,8-3

8.2 Home Page (Run in Progress),8-5

8.3 Set Speed Page (No Rotor Selected),8-6

8.4 Set Speed Page (Rotor and Labware Selected),8-6

8.5 Select Rotor and Labware Page (Library),8-7

8.6 Set Time Page,8-8

8.7 Delay Start Page,8-9

8.8 Set Speed ω²t Time Page,8-10

8.9 Set Temperature Page,8-11

8.10 Set Acceleration/Deceleration Profiles Page,8-12

8.11 Login Page,8-14

8.12 Select Program Page,8-15

8.13 New Program Page,8-16

8.14 Edit Program Page,8-16

8.15 View Program Page,8-17

8.16 Select Rotor and Labware Page (Catalog),8-18

8.17 New Step Page,8-19

8.18 Edit Step Page,8-19

8.19 Program Log Page,8-20

8.20 Export Page,8-21

8.21 Authorize Users Page,8-22

8.22 Import Page,8-23

8.23 Real-Time Run Data Page,8-24

8.24 Historical Run Data Page,8-24

8.25 Run Graph Button,8-25

8.26 Run Graph Options Page,8-25

xxvi

8.27 Run History Page,8-26

8.28 Run History Page (E-Signature Enabled),8-27

8.29 Run History Button,8-27

8.30 Run History Filter Page,8-28

8.31 Before Run Comment Page,8-29

8.32 Menu Page,8-30

8.33 System Options Page, Basic Tab,8-31

8.34 System Options Page, System Tab,8-32

8.35 System Options Page, Network Tab,8-33

8.36 System Options Page, Users Tab,8-34

8.37 System Options Page, Reports Tab,8-35

8.38 Select Language Page,8-36

8.39 User Options Page,8-37

8.40 Reset User PIN Page,8-38

8.41 Select Image Page (Avatar),8-39

8.42 Select Image Page (Background),8-39

8.43 Set Date and Time Page,8-40

Illustrations

8.44 System Log Page,8-41

8.45 Manage Rotors Page,8-42

8.46 Add to Rotor Library Page,8-43

8.47 Diagnostic History Page,8-44

8.48 Set Sound Page,8-45

8.49 Custom Sounds Page,8-46

8.50 Archive Data Page,8-47

8.51 Setup Network Page,8-48

8.52 Select Printer Page,8-49

8.53 Setup Email Page,8-50

8.54 Setup VNC Page,8-51

8.55 Manage Users Page,8-52

8.56 Add User Page,8-53

8.57 Authorize Programs Page,8-54

8.58 References Page,8-55

8.59 Rotor Catalog Page,8-56

8.60 Compatible Tubes for Rotor Page,8-57

8.61 Labware Catalog Page,8-58

8.62 Chemical Resistance Page,8-59

8.63 Calculations Page,8-60

8.64 Reduce Rotor Speed for Dense Solutions Page,8-61

8.65 Reduce Rotor Speed for Precipitating Solutions Page,8-62

8.66 Determine Sedimentation Coefficient from Run Data Page,8-63

8.67 Determine Sedimentation Coefficient from Molecular Mass

xxvii

Illustrations

Page,8-64

8.68 Calculate Pelleting Time Page,8-65

8.69 Calculate Concentration Measures Page,8-66

8.70 Calculate Refractive Index Page,8-67

8.71 About Page,8-68

8.72 Zonal Authorization Page,8-69

8.73 Zonal Mode Button,8-69

8.74 Zonal Operation Page,8-70

8.75 Continuous Flow Operation Page,8-70

8.76 Simulations Page,8-72

8.77 ESP RNA Pelleting in CsCl with GuSCN, Optimized for Purity
Page,8-73

8.78 Select Rotor and Labware Page (Catalog/Library),8-74

8.79 ESP RNA Pelleting in CsCl with GuSCN, Optimized for Speed
Page,8-75

8.80 ESP Pelleting Separation Page,8-76

8.81 ESP Optimized Plasmid DNA Separation Page,8-78

8.82 ESP Rate Zonal Separation Page,8-79

8.83 Substitute Rotor Run Page,8-80

9.1 Panel screws and Tabs,9-5

9.2 Internal Parts — Configuration “A” vents through the port
cap,9-6

9.3 Internal Parts — Configuration “B” vents through the vacuum
solenoid release screw,9-7

A.1 Safety and ventilation space,A-2
A.2 Electrical Connection,A-3

xxviii

Tables

4.1 User Levels,4-5
A.1 Required Wire Connections,A-3
C.1 Diagnostics/User Messages Chart,C-1

Tables

xxix

Tables

xxx

The Optima XPN generates centrifugal forces for the separation of particles. Classified S, it can be
used with all currently manufactured Beckman Coulter rotors for floor-model preparative
ultracentrifuges.

This chapter describes the major components of the instrument.

Touch Screen

The touch screen is both the information display and the control input for the instrument. As they
are needed, control buttons appear on the screen. When you select a button, you activate that
control.

The touch screen position is adjustable (both swivel and tilt) to be made viewable from nearly
anywhere in the lab. This is especially useful in environments where a single operator may oversee
a number of instruments.

CHAPTER 1

Description

Each component of the touch screen interface is explained in the following chapters.

Name Rating Plate

A name rating plate is affixed to the rear of the instrument. Always mention the serial number and
model number (available on the About Page ) when contacting Beckman Coulter regarding your
instrument.

Rotor Chamber

The rotor chamber is made of aluminum and coated with a chemical-resistant epoxy finish. The
central feature is the rotor drive spindle, but several sensors and control systems are also contained
in the rotor chamber.

Vacuum System

The Optima XPN uses a diffusion pump in series with a mechanical vacuum pump to reduce
chamber pressure to a very low level. The system starts automatically when you start a run, or
manually when the chamber door is closed and you use the
Bar of the Home Page. When the vacuum system is on, the chamber pressure is displayed in microns

Vacuum Display/Button.

in the

Vacuum Display/Button on the Footer

PN B08115AF

1-1

Description

Temperature Sensing and Control

At the end of a run, select the Vacuum Display/Button to vent the chamber vacuum before
attempting to open the door. When the chamber vacuum is reduced to the point that you can open
the door, the system sounds an audible tone. (See Audible Sounds.) After you open the door, there
is a five minute period where the chamber temperature remains as set (to allow back-to-back runs).
Then the chamber returns to approximate room temperature to minimize condensation collecting
in the chamber. To help keep the chamber dry and clean, keep the door closed whenever possible.
If you wish to vent the chamber before the rotor has come to a complete stop, you can do so as soon
as it slows below 3000 rpm.

In Zonal or Continuous Flow operation, the door may remain open while the rotor is spinning up to
the loading speed. The vacuum system is activated when the door is closed and the rotor speed is
above 3000 rpm (after loading the rotor). Normally, you vent the chamber at the end of the run when
the rotor has decelerated to the unloading speed, but you can vent the chamber when the rotor has
decelerated below 3000 rpm if the unloading speed is slower than that.

Temperature Sensing and Control

A solid state thermopile in the bottom of the rotor chamber monitors rotor temperature.

The Optima XPN uses a solid state thermoelectric refrigeration and heating system without coolant
or water. Cooling is provided by forced air from the fans.

When the power is on, the temperature control system starts when the door is closed and the
vacuum system is running.

Drive

The frequency-controlled, air-cooled, direct-drive induction motor requires no gears or brushes. In
addition, the drive does not require an oil vacuum seal, external oil reservoir, or continuously
operating damper. Externally cooled by forced air and internally cooled by oil, the drive delivers
ultra-smooth, quiet performance, with high rotor-imbalance tolerance.

Safety Features

The Optima XPN ultracentrifuge has been designed and tested to operate safely indoors at altitudes
up to 2000 m (6562 ft.).

Door

1-2

The high-strength structural steel chamber door has a solenoid interlock to prevent operator
contact with a spinning rotor. When the door is closed and a run begins, it locks automatically. It can
be opened only when the power is on and the rotor is at rest with the chamber at atmospheric
pressure. If there is a power failure, you can manually unlock the door as described in the

Maintenance and Troubleshooting chapter.

PN B08115AF

Barrier Ring

A 41-mm (1.63-in.) steel alloy armor ring acts as the primary barrier, surrounded by a 13-mm
(0.5-in.) vacuum chamber to provide full protection for the operator.

Imbalance Detector

An imbalance detector monitors the rotor during the run, causing automatic shutdown if rotor
loads are severely out of balance. Even at low speeds, an incorrectly loaded rotor can cause an
imbalance.

Overspeed System

The overspeed system is a safety feature designed to ensure that the rotor does not exceed its
maximum allowable speed. This system includes a photoelectric device in the rotor chamber next
to the drive spindle and an overspeed disk on the bottom of the rotor. Individual rotor manuals
provide information on the correct overspeed disks to be used with each rotor.

Description

Specifications

1

The overspeed disk has alternating light and dark sectors. As the rotor spins, the passage of
reflecting and non-reflecting sectors over the photoelectric device generates a series of pulses
detected by the electronic circuitry and software.

After the rotor reaches 600 rpm, the set speed is checked against the overspeed disk. If the set speed
is greater than the maximum speed permitted by the disk, the speed setting is automatically
lowered to the maximum speed of the disk, but the run continues without interruption. An alert
appears to alert you to the change.

Dynamic Rotor Inertia Check (DRIC)

As the rotor accelerates between 15000 and 20000 rpm, the instrument checks the rotor inertia and
calculates the rotor energy for the speed set by the user. If the calculated rotor energy is excessive,
the instrument first attempts to reduce the speed to an appropriate level and continues the run. The
system displays an alert message to notify you of this change. If a safe speed cannot be determined,
the instrument stops the run with braking to avoid possible damage.

Specifications

Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data,
without guarantee.

PN B08115AF

1-3

Description

Specifications

Control Features

Specifications Description

Speed • Set speed:

— 1,000 to rated speed in 100-rpm increments

• Maximum speed:
— XPN-80: 80,000 rpm
— XPN-90: 90,000 rpm
— XPN-100: 100,000 rpm

• Speed display:
— Actual measured rotor speed in 10-rpm increments below 1000 rpm

and 100-rpm increments ≥1000 rpm

• Speed control:
— Actual measured rotor speed, ± 2 rpm at steady state (1,000 rpm to

rated speed)

• Acceleration:
— 10 profiles

• Deceleration:
— 11 profiles, including coast

• Braking:
— Regenerative, power reclamation

Time • Set time:

— Up to 999 hours 59 minutes, including Hold runs

— Up to 3.94780 x 10

• Time display:
— Indicates time remaining in timed runs, time elapsed in Hold runs,

or estimated time remaining in ω²t runs

• Time accuracy:
— ± 70 ppm (6 seconds/day)

• Run modes:
— Time, ω²t, and RCF

14

radians squared per second in ω²t mode

1-4

PN B08115AF

Specifications Description

Temperature • Set temperature:

— 0 to 40°C in 1°C increments

• Rotor Temperature (after equilibration):

— ± 0.5°C over entire set temperature range for all rotors except

SW-32

— ± 0.8°C over entire set temperature range for SW-32

• Temperature display:

— Actual rotor temperature in 0.1°C increments

• Ambient temperature range:

— 10 to 35°C

• Humidity restrictions:

— <80% at <35°C (non-condensing)

• Condensation removal:

— Automatic, begins five minutes after vacuum vent

• Vacuum:

— Below 5 microns (0.7 Pa)

Description

Specifications

1

Ease of Use • Languages:

— Multi-language support

• Help:

— On-screen, context sensitive

• Diagnostic messages:

— 10,000 messages saved

Data • Networking:

— RJ-45 connector

• Remote Control:

— Via standard IP protocol

• Data Transfer:

— 3 USB 2.0 type A connectors

• Run Graphs:

— Speed and temperature vs. time, 5,000 graphs saved

• Run Logs:

— 5,000 logs saved

Electronic Recordkeeping • Users:

— 50 unique users and PINs

• Levels of Access:

— Administrator, Super User, and Operator

• Rotor Library:

— 75 unique rotors by serial number

PN B08115AF

1-5

Description

Specifications

Physical Data

Specification Description

Dimensions • Width :

Ventilation Clearances • Sides

— 94.0 cm (37 in.)

• Depth:
— 68.1 cm (26.8 in.)

• Height:
— 125.7 cm (49.5 in.)

• Weight
— 485 kg (1068 lb)

— 5.1 cm (2.0 in.)

• Rear
— 15.2 cm (6.0 in.)

Finishes • Rotor pad:

— Coated polycarbonate

• Top and Front surfaces:
— Polyurethane

• Other surfaces:
— Acrylic baked enamel

Electrical • Power requirement:

— 200–240 VAC, 30A branch circuit, 50/60 Hz
— Automatic tap selection on every run

• Electrical supply:
— Class I

• Power Consumption:
— 60W in idle
— 1.0 kW average running in steady state at 90K rpm

• Installation (overvoltage) category:
— II

• LCD Monitor:
— Wide tilt range, horizontal and vertical

1-6

PN B08115AF

Specification Description

Outputs • Power Factor:

— 0.99

• Nominal Output Voltage:

— 200/240

• Output Frequency:

— 50/60 Hz

• Max Output Current:

— 8.5A

• Max Fault Current:

— 20A

Environmental • RoHS compliant material:

— 100% of content

• Cooling system:

— Thermoelectric (no CFC gases used)

• Noise output (1m in front of instrument):

— <51 dBA (Sound pressure level)
— 7.0 sones (Zwicker Loudness)

• Maximum heat dissipation into room under steady-state conditions:

— 3400 Btu/hr (1.0 kW)

• Pollution degree:

a

— 2

• HEPA filter:

— Available

Description

Specifications

1

a. Normally, only non-conductive pollution occurs; occasionally, however, a temporary conductivity caused by condensation

must be expected.

Audible Sounds

The Optima XPN instrument makes an audible sound for the following events:

• Boot up

• Start of Run

• End of Run

• Diagnostics/Alert

• Vacuum low enough to open door

• Door open during Zonal or Continuous Flow mode

Sound volume can be regulated through the Set Sound Page, and some sounds can be customized
through the System Options. See Custom Sounds Page.

For safety, the Door Open during Zonal or Continuous Flow mode sound cannot be changed or
muted. It will play every 5 seconds when the door could be open.

PN B08115AF

1-7

Description

Available Rotors

Available Rotors

All currently manufactured Beckman Coulter floor model ultracentrifuge rotors can be used in the
Optima XPN ultracentrifuge. The rotors are described in individual manuals that accompany each
rotor. Information on rotors and accessories is available in the Rotor Catalog accessible from the
touch screen. Additional information is available in Rotors and Tubes for Preparative Ultracentrifuges
(LR-IM) and in the Beckman Coulter Ultracentrifuge Rotors, Tubes & Accessories catalog (publication
BR-8101). The Beckman Coulter website, http://www.beckmancoulter.com, contains additional
information about centrifugation processes and products.

Refer to specific rotor manuals for proper installation and removal of rotors from the instrument.

1-8

PN B08115AF

CHAPTER 2

The Touch Screen Interface

This chapter describes the touch screen interface for the Optima XPN. Except for the power switch,
all the controls for the instrument are programmed controls that appear on the touch screen.

Figure 2.1 Home Page

Areas on the Screen

The items on the screen may change, depending on the state of the instrument. There are three
areas where items appear on the screen:

• The Header Bar

• The Footer Bar

• The Page Display Area

Header Bar

Figure 2.2 Header Bar

The area across the top of the screen is called the Header Bar, containing four important items:

Home Page Button

•

•

Menu Button

PN B08115AF

2-1

The Touch Screen Interface

Areas on the Screen

• Status Display

Help Button

•

The following sections explain these items.

Home Page Button

Figure 2.3 Home Page Button

The Home Page Button displays the Home Page from any other page. When you use Zonal Mode or
Continuous Flow Mode, the

Menu Button

Figure 2.4 Menu Button

Zonal/Continuous Flow Operation Page replaces the Home Page.

The Menu Button displays the Menu page, including the following functions:

• Options

• References

• Calculations

• About

• Zonal Operation

• Continuous Flow Operation

• Simulations

• Service Mode

The menu options are explained in the following chapters.

Status Display

Figure 2.5 Ready Status

2-2

The Status Display is in the middle of the Header Bar and shows you the current instrument status.
The background color of the header bar changes with the type of status:

• Blue background: system ready (no run in progress).

PN B08115AF

Figure 2.6 Header Bar - Ready Status

• Green background: in operation (run in progress).

Figure 2.7 Header Bar - Running Status

• Green background: in operation (stop in progress).

Figure 2.8 Header Bar - Stopping Status

The Touch Screen Interface

Areas on the Screen

2

• Yellow background: warning message.

Figure 2.9 Header Bar - Warning Message

• Red background: error message.

Figure 2.10 Header Bar - Error Message

Help Button

Figure 2.11 Help Button

Footer Bar

PN B08115AF

The Help button gives you access to the built-in online help messages. Help messages are described
at the end of this chapter.

The bottom of the touch screen always shows the footer bar. The footer bar appears in two different
ways, but it always has the

Start Button at the left and the Stop Button at the right.

2-3

The Touch Screen Interface

Areas on the Screen

Start Button

Figure 2.12 Start Button

The Start Button begins a run with the current settings. Use it only after you have set the
parameters for the run.

Stop Button

Figure 2.13 Stop Button

The Stop button stops the current run immediately. This is ordinarily only used for emergencies or
if you mistakenly set too long a run time.

2-4

PN B08115AF

Footer Bar on the Home Page

On the Home Page, the middle of the Footer Bar shows three Items:

The Touch Screen Interface

Areas on the Screen

2

• The

Vacuum Display/Button

• The System Name

• The

Figure 2.14 Footer Bar - Home Page

Accel and Decel Display/Button

Each of these is explained below.

Footer Bar on Other Pages

When not on the Home Page, the three buttons for Speed, Time and Temperature appear in the
Footer Bar for easy access. To set Acceleration or Deceleration profiles, or to use the
select the
within the

• The

• The

• The

Home button to return to the Home Page. The following items are described in detail

Home Page section of the next chapter:

Set Speed Display/Button
Set Time Display/Button
Set Temp Display/Button

Vacuum button,

Figure 2.15 Footer Bar on Other Pages

Vacuum Display/Button

As a display, the Vacuum Display/Button shows the current chamber vacuum and the action that will
be performed when you select the button. As a control, it serves two functions:

• Before a run, after mounting the rotor and closing the chamber door, select this control to
evacuate the chamber and precondition the chamber to the set temperature.

• After a run, select this control to release the vacuum before opening the chamber door.

System Name

The system name displays the system name entered as one of the options on the System Options

Page.

PN B08115AF

2-5

The Touch Screen Interface

Help Messages

Accel and Decel Display/Button

These are two displays that, together, act as a single button.

As a display, each shows the selected acceleration or deceleration profile currently selected. Select
the button to display the Set Acceleration/Deceleration Profiles Page, described in CHAPTER 8.

Page Display Area

The Page Display Area is the main display area between the Header Bar and the Footer Bar. The
pages and help messages all appear in this area. The pages are all described in CHAPTER 8.

Help Messages

When you first select the Help Button, a number of new buttons appear on the screen, giving you
three options:

Item Help

• Item Help

• Global Help

• Exit from Help.

Item Help Buttons appear next to individual fields or objects. Select the button to display a brief

message describing the item. Only one help message remains on the screen at a time.

Figure 2.16 Item Help Button

2-6

PN B08115AF

Global Help

The Global Help Button appears next to the Help Button. If you select the Global Help Button, the
Page Display Area shows you a description of the current page and all the elements it contains.

Figure 2.17 Global Help Button attached to Help Button

While using Global Help, you also have some additional options:

The Touch Screen Interface

Help Messages

2

• The

Global Help page appears over a ghost image of the screen you were on when you selected

global help.

Figure 2.18 Home Page Help with ghost image

If the ghost image makes it difficult for you to read the Help Page, select the Transparency
Button in the upper right corner.

PN B08115AF

Figure 2.19 Transparency Button

The resulting opaque help page eliminates the image of the screen behind it.

2-7

The Touch Screen Interface

System References

Figure 2.20 Home Page Help with opaque screen

Select the Transparency Button again to return to the ghost screen image.

• If you need information about some other aspect of the instrument, you can select the Table of
Contents link at the bottom of the page to see the outline of all the available help pages. You
have access to all of the help pages from the Table of Contents.

Figure 2.21 Help Navigation Buttons

• To navigate through your selected help pages, use the Forward and Back buttons in the upper
left corner.

System References

The XPN system includes reference materials you may need as you operate the system:

• The Rotor Catalog Page lists detailed specifications for rotors that can be used with the XPN.

• The Labware Catalog Page lists detailed specifications for labware that can be used with

approved rotors.

• The Chemical Resistances Page lists chemical interaction between equipment and accessories
used in ultracentrifugation and various commonly used chemicals.

2-8

PN B08115AF

Follow this procedure to display the References page:

Figure 2.22 References Page

The Touch Screen Interface

About Your Instrument

2

1

Select the Menu button on the header bar to display the Menu page.

2

Select the References button to display the References page.

About Your Instrument

The system About page contains information about your instrument, including the model number,
serial number, and software version, that you need when you call Beckman Coulter Field Service.
Follow this procedure to display the About Page.

PN B08115AF

2-9

The Touch Screen Interface

About Your Instrument

Figure 2.23 About Page

1

Select the Menu button on the header bar to display the Menu page.

2

Select the About button to display the About page.

2-10

PN B08115AF

The touch screen interface for the Optima XPN simplifies operations. Although there are many
additional things you can do, this chapter outlines basic operations:

• Manual Operation

• Operations with Preconditioning

• Continuous Flow Operation

• Zonal Operation

Manual Operation

Manual operation is a simple procedure you can do from the Home Page.

Before you begin, you must know:

CHAPTER 3

Operations

• If rotor selection is required, which rotor you are using for the run.

• Acceleration and deceleration rate

• Run speed

• Length of time for the run

• Run temperature

NOTE

When you have this information, the procedure for the run is as follows:

1

2

3

4

Your system may have additional requirements, depending on your configuration.

Start on the Home Page.

Set the speed and rotor.

Set the Acceleration and Deceleration Profiles.

Set the time.

PN B08115AF

5

Set the temperature.

3-1

Operations

Manual Operation

6

Start the run.

Step 1: Start on the Home Page

Start at the Home Page. If your screen shows any other page, select the Home Page Button in the
upper left corner of the screen.

Figure 3.1 Home Page

3-2

NOTE If your system is in Zonal Mode or Continuous Flow Mode, you must select the Cancel Button to go to

the Home Page.

PN B08115AF

Figure 3.2 Zonal Operation Page with Cancel Button

Operations

Manual Operation

3

Step 2: Set the Speed and Rotor

Select the Set Speed Display/Button on the Home Page to go to the Set Speed Page.

Figure 3.3 Set Speed Page

PN B08115AF

3-3

Operations

Manual Operation

When the Set Speed Page appears, follow these steps:

1

If you need to record rotor selection, select the Select Rotor Button to go to the Select Rotor and

Labware

Figure 3.4 Select Rotor and Labware Page

Page.

2

Select the rotor and labware for the run from the library. If the desired rotor is not in the library,
a system Administrator or Super User must add it.

3

Select the OK Button to return to the Set Speed Page.

4

If you plan to set the speed in units of relative centrifugal field (RCF), select the RPM/RCF Button.
Note that the RPM/RCF button is only enabled when you have selected a rotor.

5

Use the keypad to set the desired speed. Note that you actually enter hundreds, with two ending
zeroes added to your number. You can also use the

6

Select the OK key to accept your entry and dismiss the page.

Back and Clear keys to make corrections.

3-4

PN B08115AF

Step 3: Set the Acceleration and Deceleration Profiles

Select the Accel and Decel Display/Button to go to the Set Acceleration/Deceleration Profiles Page.

Figure 3.5 Set Acceleration/Deceleration Profiles Page

Operations

Manual Operation

3

On the Set Acceleration/Deceleration Profiles Page, follow these steps:

1

Select the desired Acceleration and Deceleration profiles.

2

Select the OK key to accept your entries and dismiss the page.

PN B08115AF

3-5

Operations

Manual Operation

Step 4: Set the Time

Select the Set Time Display/Button to go to the Set Time Page.

Figure 3.6 Set Time Page

On the Set Time Page, follow these steps:

1

Use the keypad to set the desired time in hours and minutes. Use the Back and Clear keys to
make corrections. You can also use the

countdown to an automatic end. When you select

Stop key (or until the maximum time of 999 hours and 59 minutes has been reached).

2

You can use the Delay Start button to set a future start or stop time. Select Delay Start to display
the Delay Start Page.

Hold key to set the time to a hold state with no

Hold, the run does not end until you select the

3-6

PN B08115AF

Figure 3.7 Delay Start Page

Operations

Manual Operation

3

• Select Start At or Stop At to set a starting or stopping time. Then you can set the date and
time in the fields above. Select OK to return to the

3

Select the OK key to accept your entry and dismiss the page.

Step 5: Set the Temperature

Select the Set Temp Display/Button to go to the Set Temperature Page.

Set Time Page.

PN B08115AF

3-7

Operations

Manual Operation

Figure 3.8 Set Temperature Page

On the Set Temperature Page, follow two steps:

1

Use the keypad to set the desired temperature in degrees Celsius. Use the Back and Clear keys
to make corrections.

2

Select the OK key to accept your entry and dismiss the page.

3-8

PN B08115AF

Step 6: Start the Run

Once the run values are set, start the run:

Operations

Manual Operation

3

NOTE

1

2

3

4

5

Figure 3.9 Start Button

If you have entered a delayed start, you must select the Start button to begin the countdown to the

delayed start.

Prepare your samples and place them in the rotor following all proper procedures, including
balanced weight distribution.

Preheat or precool the rotor and samples, if necessary.

Mount the rotor in the instrument following all the procedures in the rotor manual and
observing all safety procedures and cautions.

Close and lock the chamber door. For preconditioning, select the Vacuum Display/Button and
wait until the chamber reaches the set temperature.

Select the Start button.

PN B08115AF

When the run begins, wait for the countdown timer to reach zero and the rotor to come to a stop.
You can then remove your rotor and samples.

3-9

Operations

Zonal and Continuous Flow Operation

Zonal and Continuous Flow Operation

WARNING

In zonal and continuous flow operation, the operator is unavoidably exposed to
rotating machinery. For safety, the operator must be properly instructed and
qualified. Guard against accidentally dropping objects, such as pens, pencils, or
hemostats into the chamber. Loose lab coats, neckties, scarves, and long
necklaces should not be worn while operating in the zonal or continuous flow
mode.

Use only zonal rotors in the zonal mode.

Use only continuous flow rotors in the continuous flow mode.

Zonal and Continuous Flow operations require special rotors and have some additional hazards in
that samples are loaded and unloaded while the rotor is spinning. To prevent unauthorized users
from attempting these operations, the instrument requires an authorization code (which is 1793).
When Zonal or Continuous Flow operations are authorized, the system displays the
Page or the
Page button displays the corresponding Zonal or Continuous Flow Operation page. The mode does
not end until the run is complete or the user selects the

Continuous Flow Operation Page. While in Zonal or Continuous Flow operation, the Home

Zonal Operation

Cancel Button to end the mode.

Zonal and Continuous Flow operations are very similar. The specific details vary according to the
rotor you are using, but the summary below shows an outline of the procedure.

Figure 3.10 Zonal Page

There are seven phases to a Zonal or Continuous Flow run:

• Preparing

• Starting

• Loading

• Running

3-10

• Unloading

PN B08115AF

• Stopping

• Finishing

Each phase is outlined in a section below.

Preparing for the Run

The rotor manual details the preparations for a run, which may include cleaning, assembling, and
lubricating the rotor, setting up pumps and tubing, and usually includes overnight cooling to
precondition the rotor, sample and solutions used in the run. When you are ready to install the
rotor, set the parameters for the run:

• Run Speed

• Run Time

• Temperature

• Load Speed

• Unload Speed

Operations

Zonal and Continuous Flow Operation

3

For Continuous Flow operation, you may have two different load speed settings. The initial setting
is for alignment and adjustment checks, which you set in this phase.

When everything else is ready, mount the rotor and select the Vacuum Display/Button to begin
preconditioning the chamber. When the chamber reaches the set temperature, you are ready to
start the run.

Starting the Run

To start the run, select the Start Button and wait for the rotor to reach loading speed. The status
display at the top of the screen highlights Starting.

For a Continuous Flow run, you need to perform alignment and adjustment checks as detailed in the
rotor manual. You may need to use the
with the
checks, set the new loading speed (if it is different from the adjustment speed) and select the
Button again.

Slow to Zero RPM button to make adjustments and restart

Start Button. Refer to Figure 3.11. When the rotor passes the alignment and adjustment

Start

PN B08115AF

3-11

Operations

Zonal and Continuous Flow Operation

Figure 3.11 Continuous Flow Page

When the rotor reaches the Loading speed, the status display highlights Loading, to show that you
have moved into the next phase.

Loading the Sample

In the Loading phase, you inject the sample into the medium in the rotor. Again, the details depend
on the rotor and the type of operation as detailed in the rotor manual. When you have finished all
the specified loading steps, close the door and select the
phase.

Running the Sample

The instrument highlights Running on the Status Display, accelerates to the set run speed, and
begins counting down the set run time. The run phase ends in one of three ways:

• When the timer countdown reaches zero, the instrument decelerates to the unloading speed
and goes to the Unloading phase.

• If you select the
unloading speed and goes to the Unloading phase.

• If you select the
the unloading phase, and exits Zonal or Continuous Flow mode entirely.

Unloading the Sample

Loading Complete button to go to the next

Unload Button to terminate the run early, the instrument decelerates to the

Stop Button to abort the run, the instrument brings the rotor to a halt, skips

3-12

While the rotor is running at unload speed, follow the instructions in the rotor manual to inject the
displacement solution into the rotor and collect the resulting centrifugate sample.

PN B08115AF

Stopping the Run

When you have completed all the defined unloading steps, select the Stop Button to bring the rotor
to a halt.

Finishing the Run

When the rotor comes to a halt, unmount it and perform all the cleanup and follow-up steps given
in the rotor manual. The instrument exits Zonal or Continuous Flow mode when the rotor comes to
a stop.

Operations

Zonal and Continuous Flow Operation

3

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3-13

Operations

Zonal and Continuous Flow Operation

3-14

PN B08115AF

The Optima XPN includes the many options for configuring your system. Some of these options
have a significant effect on system functionality, and must be configured before you begin to use the
system. These options include:

• Managing Your Network

• Managing Users

• Managing Rotors

• Managing Reports

Managing Your Network

You can add the Optima XPN to your network, enabling email, file transfer, and printing to a
network printer. You can also enable VNC (Virtual Network Connection) or API (Application
Programming Interface) to allow the system to receive instructions from a remote application.

CHAPTER 4

Configuration

Setting Up the Network

1

Select the Menu button on the Header Bar to display the Menu page.

2

Select Options to display the System Options page.

3

Select the Network Tab, then select Setup Network to display the Setup Network page.

4

Network path is the path the instrument automatically uses for import and export. Select the
Network Path field to display the Export Path page and enter the default path. Select OK to

return to the Setup Network page.

5

DHCP Mode (Dynamic Host Configuration Protocol) is enabled by default and automatically

configures the IP addressing parameters using a DHCP Server on the network.
If your network administrator provides a specific IP address, disable DHCP mode and enter the

values provided for the following fields:

• IP Address

• Subnet Mask

• Default Gateway

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4-1

Configuration

Managing Your Network

• DNS Server

To disable DHCP Mode, select

6

Select Save to save the network information and return to the System Options page.

Selecting a Printer

Use this option to select a USB or network printer for the instrument. The system automatically
sends all print requests to the selected printer.

NOTE

1

2

3

Printer drivers must be installed by a Beckman Coulter Field Service representative.

Select the Menu Button on the Header Bar to display the Menu page.

Select Options to display the System Options page.

Select the Network Tab, then select Select Printer to display the Select Printer page, which lists
the available printers.

Enable so that the green square is cleared.

4

Select a printer from the list. You can select Test Print to send a test page to the printer.

5

Select Save to save the printer selection and return to the System Options page.

4-2

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Setting Up Email

Use this option to configure email that can be sent from the instrument. The instrument will send
diagnostic notifications to the email account of all users with an email address entered in their user
profile.

1

Select the Menu button on the Header Bar to display the Menu page.

2

Select Options to display the System Options page.

3

Select the Network Tab, then select Setup Email to display the Setup Email page.

4

Select SMTP Server to enter your email server address. Select OK to save the address and return
to the

Setup Email page.

Configuration

Managing Your Network

4

5

Port Number defaults to 25. Do not change it unless you must use another, specific port number.

6

User Name and Password are optional, but may be required by your email server. Select the

fields to enter the required values, then select

7

Email From defines the return email address that appears on email notifications sent by the

instrument. You can change the default to a legitimate or fictitious address, depending on your
requirements. Select the field to enter the new address, then select
return to the

8

Select the SSL Server Enable button to enable email encryption, if required by your email server.
The button displays a green square when the option is enabled.

9

You can select Test Email to send an email to test your configuration. Enter the recipient email
address and select OK to send the email and return to the

a status message for the success or failure sending the test email.

10

When you have completed your configuration, select Save to return to the System Options page.

Setup Email page.

OK to return to the Setup Email page.

OK to save the address and

Setup Email page. The system displays

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4-3

Configuration

Managing Your Network

Setting Up VNC

Use VNC (Virtual Network Connection) to connect to the instrument from a laptop or other remote
device.

1

Select the Menu Button on the Header Bar to display the Menu page.

2

Select Options to display the System Options page.

3

Select the Network Tab, then select Setup VNC to display the Setup VNC page.

4

Select the Enable or Disable VNC Server Enable button to enable the VNC server. The button
displays a green square when the option is enabled.

5

6

Enabling API

Use this option to allow a remote device to connect to the instrument using the API.

1

2

3

To use the PIN of the current user as the VNC password, select the Synchronize VNC Password
To Logged in User PIN

Enter and confirm the password and select

Select Back to return to the System Options page.

Select the Menu Button on the Header Bar to display the Menu page.

Select Options to display the System Options page.

Select the Network Tab, then select Enable API. The button displays a green square when the
option is enabled.

Enable button. To enter a separate VNC password, select Set Password.

OK to save and return to the Setup VNC page.

4-4

4

Select Done to return to the Home page.

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Managing Users

You can require users to log in prior to using the Optima XPN. You can use the login to build a run
log for each user, and to control access to the system.

The system includes three user levels that grant different ranges of system access.

Table 4.1 User Levels

User Level Permission

Operator Can run assigned programs and change user options.

Super User Can run all programs, run the XPN manually, manage users, assign

Administrator Unlimited access.

programs, manage the rotor library, perform calculations and
simulations.

Configuration

Managing Users

4

Adding Users

1

2

3

4

5

6

7

To add users to the system, select the Menu Button on the Header Bar to display the Menu Page.

Select Options to display the System Options Page.

Select the Users Tab, then select Manage Users to display the Manage Users page.

Select Add to display the Add User Page.

Select the User ID field to display the Edit User ID Page.

Use the keypad to enter the new User ID. Select OK to return to the Add User page.

Repeat the procedure for the remaining fields. The PIN and Full Name fields are required.

PN B08115AF

8

Select the User Level. For Operators, you can select the Authorize Programs button to add
programs to the User. See Creating Programs for more information.

9

Select Save to add the user to the system and return to the Manage Users Page.

4-5

Configuration

Managing Users

You can also use the Manage Users page to edit or delete users, to copy existing user information
for a new user, or to edit program permissions for Operator-level users.

Requiring Login

1

Select the Menu Button on the Header Bar to display the Menu Page.

2

Select Options to display the System Options Page.

3

Select the Users Tab, then select Require Login. The button displays a green square when the
option is enabled.

4-6

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PIN Expiration and Logout Timer

The system defaults to expire PINs every 60 days, and to log out users after two minutes of inactivity.
Follow these steps to change the defaults.

1

Select the Menu Button on the Header Bar to display the Menu Page.

2

Select Options to display the System Options Page.

3

Select the Users Tab.

4

To change the PIN expiration, select the PIN Expiration button.

5

Select Clear to clear the field. Enter the number of days that you want PINs to remain valid. To
disable PIN Expiration, enter 0.

Configuration

Managing Users

4

6

Select OK to return to the Users Tab.

7

To change the logout time, select the Logout Timer button.

8

Change the field to the number of minutes of inactivity before the system logs out a user. To
disable the Logout Timer, enter 0.

9

Select OK to return to the Users Tab.

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4-7

Configuration

Managing Rotors

User Options

When Login is required, the User Options page becomes active. From this page, users can change
their PIN, add or change their email and phone number, and select an avatar and background to
appear on their Home page.

1

2

3

4

Select the Menu button on the Header Bar to display the Menu page.

Select Options to display the System Options page.

Select the Basic Tab, then select User Options. They system displays the User Options page for
the user that is currently logged in.

To change the PIN, select the PIN field to display the Reset User PIN page.

• Use the keypad to enter the current PIN, and confirm the new PIN.

• Select

5

To add or change the email or phone number, select the Email or Phone field to display the Edit

Email

• Use the keypad to update the field.

• Select

6

To select or change the avatar or background, select Set Avatar or Set Background to display the

Select Image Page.

• Select an image from the available images on the left, or select

• Select

7

Select an image from the available images on the left, or select Import Image to import an image
from a USB device or network location.

Save to change the PIN and return to the User Options Page.

or Edit Phone page.

Save to save your changes and return to the User Options Page.

Import Image to import an

image from a USB device or network location.

Save to set the image and return to the User Options Page.

8

Select Done to return to the System Options page.

Managing Rotors

When you enter a manual run or create a program, you can record the rotor that you are using, and
thus track rotor usage and permit conversion from RPM to RCF.

4-8

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When you enable Require Rotor Selection, all users must select a rotor from the rotor library before
a run.

To make rotors available for selection, you must add rotors to the rotor library

Adding Rotors

1

2

3

Configuration

Managing Rotors

To add rotors to the rotor library, select the Menu button on the Header Bar to display the Menu
page.

Select Options to display the System Options page.

Select the System Tab, then select Manage Rotors to display the Manage Rotors page.

4

4

Select Add to display the Add to Rotor Library page.

5

Select a type of rotor from the list on the left.

6

You must enter a serial number for the rotor. Select the Serial Number field to display the Serial

Number

7

If you want to enter an existing run count, repeat the procedure for the Run Count field. The run
count will increment automatically as the rotor is used.

8

Select Save to add the rotor to the Rotor Library and return to the Manage Rotors Page.

You can also use the Manage Rotors page to delete rotors from the library.

Page. Enter the serial number and select OK to return to the Add to Rotor Library page.

Requiring Rotor Selection

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1

Select the Menu button on the Header Bar to display the Menu page.

2

Select Options to display the System Options page.

3

Select Require Rotor Selection. The button displays a green square when the option is enabled.

4-9

Configuration

Managing Reports

Managing Reports

Optima XPN automatically saves run history data associated with each run. The run history
generated includes:

• User information

• Start date and time of the run

• End date and time of the run

• Rotor type

• Labware (tube)

• Program name

• Acceleration and Deceleration parameters

• Before Run Comment

• After Run Comment

• Detailed information about each step in a run

• Graph of the run

User Access

• An Operator-level user can manually filter data, graph data, and print data for a run.

• A Super User-level user can also export run history data to an external media source, such as a

• An Administrator-level user can also configure Optima XPN to automatically print and/or

Filter Data

You can filter run history data before you graph or print it.

1

Figure 4.1 Run History Button

2

USB drive or network location.

export run history data.

On the Home page, select the Run History button on the side menu.

On the Run History page, select the Filter tab at the bottom of the screen.

4-10

3

On the Run History Filter page, select the Filter by User button and choose from the options
below.

PN B08115AF

Figure 4.2 Run History Filter

Configuration

Managing Reports

4

4

Select the Filter by Date button, then select the From button.

Figure 4.3 Set From Date and Time

5

On the Set From Date and Time page, use the up and down arrow buttons to set the parameters
for the Month, Day, Year, Hour, Minute, and AM/PM. Then select OK to return to the

Filter

page.

Run History

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6

Select the To button under Filter by Date.

7

On the Set To Date and Time page, again use the up and down arrow buttons to set the
parameters for Month, Day, Year, Hour, Minute, and AM/PM (depending on selected time

format). Then select OK to return to the

Run History Filter page.

4-11

Configuration

Managing Reports

8

Figure 4.4 Run History

On the Run History Filter page, select OK to return to the Run History page.

You can now Print, Graph or Export the filtered run history.

Graph Data

To graph data for a run in progress:

1

Figure 4.5 Run Graph Button

2

3

To graph data for a previously completed run:

Select the Run Graph button on the side menu of the Home page.

On the Real-Time Run Data page, select Options.

On the Run Graph Options page, select one of three options under Run Graph View Scope: Fit To

Screen

, Auto Scroll, or Manual Scroll. The default is Fit To Screen. Then select OK.

4-12

1

Select the Run History button on the side menu of the Home page.

PN B08115AF

Configuration

Managing Reports

Figure 4.6 Run History Button

2

On the Run History page, select the Date of the run from the left column.

3

Select the Graph tab at the bottom of the page.

4

On the Historical Run Data page, select Options.

5

On the Run Graph Options page, choose one of three options: Fit To Screen, Auto Scroll, or Manual

. (Fit To Screen is the default.) Then select OK.

Scroll

4

Print Data

Figure 4.7 Run Graph Options

PN B08115AF

You can print run history from the Run Graph page or the Run History page.

NOTE

To print run history from the Run Graph page:

1

You must have Administrator access to configure the printer.

After the run is completed, select the Run Graph button from the side menu on the Home page.

4-13

Configuration

Managing Reports

Figure 4.8 Run Graph Button

2

3

4

On the Historical Run Data page, select the Options tab.

On the Run Graph Options page, choose one of three options: Fit To Screen, Auto Scroll, or Manual

. (Fit To Screen is the default.) Then select OK.

Scroll

Select the Print button. The system sends the run graph to the printer configured by the
Administrator.

To print run history from the Run History page:

1

Figure 4.9 Run History Button

2

3

Export Data

Run history data can be manually exported to an external media source, such as a USB drive, or
automatically exported to a network.

Select the Run History button from the side menu of the Home page.

Use filtering to display the run histories you want to print.

Select the Print button. The system sends the run history for all currently listed runs to the
printer configured by the Administrator.

4-14

NOTE

To manually export run history data to a USB drive:

1

You must have Administrator access to configure the network to automatically export data.

Select the Run History button on the side menu of the Home page.

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Configuration

Managing Reports

Figure 4.10 Run History Button

2

On the Run History page, use filtering to display the run histories you want to export, then select
the

Export button.

Figure 4.11 Run History

4

3

On the Export Run History page, the system displays a prompt to insert the USB drive.

4

When the system reads the USB drive, select it from the Available Drives list, and select the

Export button.

Optima XPN will then export the currently listed run histories to the USB drive.

To manually export run history data to a mapped network:

1

Select the Run History button on the side menu of the Home page.

Figure 4.12 Run History Button

2

On the Run History page, use filtering to display the run histories you want to export, then select
the

Export button.

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4-15

Configuration

Managing Reports

3

On the Export Run History page, select the network from the Available Drives list, then select the

Export button.

Auto Print and Auto Export Run History Data

A user with Administrator access can configure Optima XPN to automatically print run history data.
Optima XPN can also be configured to automatically export run history data to a previously
configured network. Run history data automatically sent to a network is generated as a .csv file (a
simple spreadsheet format) and an .xml file (a simple, structured text format).

IMPORTANT

write permission to a folder where the automatically exported run history data can be stored.

Before you enable Auto Export, check with your network administrator to make sure you have

To enable Auto Print:

1

Select the Menu button at the top of the Home page.

2

Select the Options button.

3

Select the Reports tab.

4

Select the Auto Print button.

The small box in the
When a run is completed, Optima XPN will automatically print the run history data to the

printer previously configured to the program.

5

To deactivate the Auto Print function, follow the previous four steps.

Auto Print button will turn green to indicate it is enabled.

4-16

The small box in the

Auto Print button will turn grey to indicate it is disabled.

To enable Auto Export:

NOTE Before you enable Auto Export, uses the Setup Network page to configure network settings. See

Setting Up the Network for more information.

1

Select the Menu button at the top of the Home page.

PN B08115AF

2

Select the Options button.

3

Select the Reports tab.

4

Select the Auto Export button.

Configuration

Managing Reports

4

The small box in the
When a run is completed, the instrument automatically exports the run history data to the

network (if previously configured to the program) as a .csv file (a simple text spreadsheet) and
an .xml file (a simple, structured text format).

5

To deactivate the Auto Export function, follow the previous four steps.

The small box in the

Run Comments

The Run Comments function enables the user to add comments to the run log before and after the
end of a run.

To enable Run Comments:

1

On the Home page, click on Menu icon.

2

Select Options.

Auto Export button will turn green to indicate it is enabled.

Auto Export button will turn grey to indicate it is disabled.

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3

Select the Reports tab.

4

Select the Run Comments button.

The small box in the

5

To deactivate the Run Comments function, follow the previous four steps.

The small box in the

To use Run Comments:

1

Select the Start button to begin a run.

Run Comments button will turn green to indicate it is enabled.

Run Comments button will turn grey to indicate it is disabled.

4-17

Configuration

Managing Reports

2

Figure 4.13 Before Run Comment

The system displays the Before Run Comment page. Enter a comment and select OK to start the
run. You can leave the comment field blank, but you must select

Cancel, the run will not start.

OK to start the run. If you select

3

When the run stops or you select the Stop button, the system displays the After Run Comment
page. Enter a comment, and select

The comments appear in the

OK.

Summary tab of the Run History page.

E-Signature

The E-Signature function permits a user to electronically add a signature and add a note (if desired)
to run history data after the run is complete.

NOTE

To enable E-Signature:

1

2

3

You must have Administrator access to enable E-Signature.

On the Home page, select the Menu button.

Select the Options button.

Select the Reports tab.

4-18

4

Select the E-Signature button.

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The small box in the E-Signature button will turn green to indicate it is enabled.

5

To disable the E-Signature function, follow the previous four steps.

Configuration

Managing Reports

4

The small box in the

To use E-Signature:

1

After a run is complete, select the Run History button on the side menu of the Home page.

2

On the Run History page, select the run to which comments will be added.

3

Select the Signature tab.

4

In the Sign As box, select Author, Reviewer, or Approver.

NOTE

Figure 4.14 Signature

You can select the Author option only if you started the run. You must have Super User or

Administrator access to select Reviewer or Approver.

E-Signature button will turn grey to indicate it is disabled.

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5

Select Sign or Add Note.

a. If you select

The system adds the E-Signature to the Summary on the

History Page for more information.

b. If you select

Sign. The system displays the Sign page. Enter your PIN and select OK.

Sign, the system displays the Sign page. Enter your PIN and select OK.

Run History Page. See the Run

Add Note, the system displays the Add Note page. Enter the note, then select

4-19

Configuration

Managing Reports

The note and E-Signature will be added to the Summary on the Run History page. See the

Run History Page for more information.

4-20

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The Optima XPN includes the functionality to create and store programs in the centrifuge memory.
A program is a series of steps containing parameters for a run. Programs are retained in the
centrifuge memory until they are deleted.

Creating Programs

1

Select Program from the side menu on the Home page.

Figure 5.1 Program Button

CHAPTER 5

Programs

Figure 5.2 Home

2

On the Select Program page, select the New button.

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5-1

Programs

Creating Programs

Figure 5.3 Select Program

3

On the New Program page, •Unnamed• appears as the title. To name the new program now,

•Unnamed• on the screen.

select

Figure 5.4 Unnamed program

4

On the New Program Name page, use the keyboard to name it, then select OK to save it.

5-2

NOTE

Program names cannot be reused.

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Figure 5.5 New Program Name

Programs

Creating Programs

5

5

On the New Program page, select the Accel Decel button to set the Acceleration and Deceleration
profiles. On the

them and return to the previous page.

Figure 5.6 Set Acceleration/Deceleration Profiles

6

To specify the rotor and labware, select the Rotor Labware button. The system displays rotor
types on the left side of the page. Select a rotor, then select the compatible labware from the list

on the right side of the page. When you are finished making your selections, select
them and return to the previous page.

Acceleration/Deceleration Profiles page, set the profiles, then select OK to save

OK to save

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5-3

Programs

Creating Programs

Figure 5.7 Select Rotor and Labware

7

On the New Program page, select the New Step button.

Figure 5.8 New Step

a. Select the Set Speed button at the top of the page, then use the keypad to set the speed. The

speed must be at least 1,000 RPM up to the maximum speed for selected rotor and labware,
or if no rotor is specified, the rated speed for the instrument.

b. Select the

Set Time button, then use the keypad to set the time range. The run time must be

between 1 minute and 999 hours 59 minutes.

c. Select the

Set Temp button, then use the keypad to set the temperature.

5-4

d. When the parameters for Speed, Time, and Temperature are set, select

and return to the

New Program page.

OK to save the step

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Running Programs

8

The new step appears in a numbered sequence for the run. From this point, more steps can be
added to the run, edited, or deleted using the

Figure 5.9 New Step in a numbered sequence

New Step, Edit Step, and Delete Step buttons.

Programs

5

Running Programs

1

Select Program from the side menu on the Home page.

Figure 5.10 Program Button

2

On the Select Program page, choose the program you want to run, then select OK.

3

Select the Start button. The run program begins.

NOTE

You must have Super User or Administrator access to change a run in progress. If you select the
Set Speed, Set Time, or Set Temp buttons to change the parameters while a program is running, the
system displays a message that you cannot change parameter values for the selected program. It
will ask if you want to exit the program and run with the changes, in which case, the remaining steps
in the program will NOT be run.

PN B08115AF

4

To stop the run for any reason, select the Stop button.

5-5

Programs

Editing Programs

Editing Programs

You must have Administrator or Super User access to modify any part of a program, which includes
the Steps, Acceleration/Deceleration rates, and Rotor and Labware. You can edit a program only
when it is not running.

To edit a program:

1

Select Program from the side menu on the Home page.

Figure 5.11 Program Button

2

Select the program you want to edit and select the Edit button. If the program is selected to run,
that selection will be cleared. If the program is running, the

3

On the Edit Program page, select the numbered step you want to edit, then select the Edit Step
button.

4

On the Edit Step page, select each button you want to modify (Set Speed, Set Time, and Set

Temp

), then select the Clear button and enter the new parameters. Select OK to save the changes

and return to the previous page.

5

On the Edit Program page, you can select the Accel Decel button and/or the Rotor Labware
button, and modify each set of parameters. Then select Save.

Deleting Programs

You can delete any program that is not currently running.

Edit button will be disabled.

5-6

1

On the Home page, select Program from the side menu.

Figure 5.12 Program Button

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Deleting Programs

2

On the Select Program page, select the program you want to delete, then select the Delete
button.

3

The system displays a confirmation message. Select Yes to delete the program, or select No to
cancel the deletion.

Programs

5

NOTE

A deleted program name cannot be reused.

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5-7

Programs

Deleting Programs

5-8

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Using Calculations

Use Calculations to perform a variety of calculations commonly used in ultracentrifugation. These
calculations help simplify run preparation.

The Calculations function includes the following options:

• Reduce Rotor Speed For Dense Solutions

• Reduce Rotor Speed For Precipitating Solutions

• Sedimentation Coefficient From Run Data

• Sedimentation Coefficient From Molecular Mass

• Pelleting Time

• Refractive Index

• Concentration Measures

CHAPTER 6

Calculations

The following sections describe how to access the Calculations Page and use the options.

Calculations Page

To display the Calculations page:

1

On the Menu Page, select Calculations.

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6-1

Calculations

Using Calculations

Figure 6.1 Menu Page

The system displays the Calculations page.

6-2

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Figure 6.2 Calculations Page

Calculations

Using Calculations

6

Reduce Rotor Speed For Dense Solutions

This function calculates the reduced run speed when centrifuging a solution with a density greater
than the allowable density rating of the rotor (as listed in the applicable rotor manual) to protect
the rotor from excessive stresses due to the added load.

To calculate the reduced speed for dense solutions:

1

On the Calculations page, select Reduce Rotor Speed For Dense Solutions.

The system displays the Reduce Rotor Speed for Dense Solutions page.

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6-3

Calculations

Using Calculations

Figure 6.3 Reduce Rotor Speed for Dense Solutions Page

2

Select Select Rotor and Labware to select a rotor and labware combination, then select OK.

3

Select Average Density to enter the average density of the tube contents in g/mL, then select OK.

The

Maximum Allowable Speed (RPM) is displayed.

4

Note the calculated speed, then select Done to return to the Calculations page.

Reduce Rotor Speed For Precipitating Solutions

This function calculates the reduced run speed to avoid precipitation of gradient material during
centrifugation, based on CsCl at 25°C.

To calculate the reduced speed for precipitating solutions:

1

On the Calculations page, select Reduce Rotor Speed For Precipitating Solutions.

The system displays the Reduce Rotor Speed for Precipitating Solutions page.

6-4

PN B08115AF

Figure 6.4 Reduce Rotor Speed for Precipitating Solutions Page

Calculations

Using Calculations

6

2

Select Select Rotor and Labware to select a rotor and labware combination, then select OK.

3

Select Average Density to enter the average density of the tube contents in g/mL, then select OK.

Maximum Allowable Speed (RPM) is displayed.

The

4

Note the calculated speed, then select Done to return to the Calculations page.

Sedimentation Coefficient From Run Data

This function calculates the sedimentation coefficient from the given run data, based upon a rate
zonal run.

To calculate the sedimentation coefficient:

1

On the Calculations page, select Sedimentation Coefficient From Run Data.

The system displays the Determine Sedimentation Coefficient From Run Data page.

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Figure 6.5 Determine Sedimentation Coefficient From Run Data Page

2

Select Select Rotor and Labware to select a rotor and labware combination, then select OK.

3

Select Material Location to enter the material location as the percentage down the tube from the
meniscus, then select

4

Select Gradient to select the gradient, then select OK.

Density gradient centrifugation is a technique used to separate proteins of different sizes from
a sample. These are the options:

• Water

• Sucrose 5% - 20%

• Sucrose 10% - 40%

• Sucrose (Custom)

Use the Sucrose (Custom) option to manually select the gradient of your choice. Once the
(Custom) option is selected, use the up or down arrow to set the limits, then select

5

Select Particle Density to enter the value for the particle density in g/mL, then select OK.

OK.

OK.

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6

Select Speed/Time/Temperature and enter the values for the speed, time, and temperature, then
select

OK.

7

Note the sedimentation coefficient, then select Done to return to the Calculations page.

Sedimentation Coefficient From Molecular Mass

This function calculates the sedimentation coefficient from the given molecular weight of the
macromolecule.

To calculate the sedimentation coefficient:

1

On the Calculations page, select Sedimentation Coefficient from Molecular Mass. The system
displays the

Determine Sedimentation Coefficient from Molecular Mass page.

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6

Figure 6.6 Determine Sedimentation Coefficient from Molecular Mass Page

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2

Select the macromolecule.

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3

Select Molecular Mass or Molecular Length to enter the molecular mass or molecular length of
the molecule, then select

OK.

NOTE

The Sedimentation Coefficient is displayed.

4

Note the calculated sedimentation coefficient, then select Done to return to the Calculations
page.

Pelleting Time

This function calculates the minimum time required to pellet a particle with a known sedimentation
coefficient in water.

To calculate the pelleting time:

1

On the Calculations page, select Pelleting Time.

The system displays the Calculate Pelleting Time page.

Figure 6.7 Pelleting Time Page

The Molecular Mass button changes to Molecular Length for the DNA or RNA macromolecules.

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2

Select Select Rotor and Labware to select a rotor and labware combination, then select OK.

3

Select Sedimentation Coefficient to enter the sedimentation coefficient of the particles being
separated, then select

4

Select Speed (RPM/RCF) to enter the speed in RPM or RCF.

OK.

6

NOTE

5

Select Calculate.

The time required to pellet the particles is displayed in the

NOTE

6

Note the calculated time, then select Done to return to the Calculations page.

Refractive Index

Calculates the values for the refractive index, density, and molarity for CsCl at 20°C.

To calculate the refractive index, density, or molarity:

1

On the Calculations page, select Refractive Index.

The system displays the Calculate Refractive Index page.

This option is not enabled until a rotor is selected.

Pelleting time (h:mm): field.

This option is not selectable until a rotor, sedimentation coefficient, and speed are selected.

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Figure 6.8 Calculate Refractive Index Page

2

Enter the value for one of the following options:

•

Refractive Index

• Density

• Molarity

The remaining two parameters are calculated and displayed.

3

Note the calculated parameters, then select Done to return to the Calculations page.

Concentration Measures

This function converts between concentration measures.

1

On the Calculations page, select Concentration Measures. The system displays the Calculate
Concentration Measures page.

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