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P H A R M A C I A B I O T E C H

MultiTemp® III

User Manual

18-1106-33

Edition AD

A

Important user information

Reading this entire manual is necessary for full understanding and safe use of this product.

Should you have any comments on this manual, we will be pleased to receive them at:

Pharmacia Biotech AB S-75182Uppsala Sweden

Pharmacia Biotech reserves the right to make changes in the specifications without prior notice.

Warranty and Liability

Pharmacia Biotech AB guarantees that the product delivered has been thoroughly tested to ensure that it meets its published specifications. The warranty included in the conditions of delivery is valid only if the product has been installed and used according to the instructions supplied by Pharmacia Biotech AB.

Pharmacia Biotech AB shall in no event be liable for incidental or consequential damages, including without limitation, lost profits, loss of income, loss of business opportunities, loss of use and other related exposures, however caused, arising from the faulty and incorrect use of the product.

Copyright© 1997 Pharmacia

Biotech AB

All rights reserved. No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form by any means, without permission in written form from the company.

Contents

1.

Introduction.....................................................................

3

2.

Safety information ...........................................................

3

 

2.1

Safety precautions.................................................

3

 

2.2

Safety standards....................................................

4

 

2.3

Safety features ......................................................

5

3.

Installation.......................................................................

6

 

3.1

Unpacking and installation...................................

6

 

3.2

Tubing connections...............................................

7

 

3.3

Recommended bath liquids...................................

7

 

3.4

Filling and draining the bath ................................

8

 

3.5

Mains connection .................................................

8

4.

Operation ........................................................................

9

 

4.1

Start-up..................................................................

9

 

4.2

Setting the temperature limit ..............................

10

 

4.3

Temperature setting limit....................................

10

 

4.4

Temperature setting ............................................

11

 

4.5

Temperature correction value .............................

11

5.

Trouble shooting..............................................................

13

 

5.1

Error messages......................................................

13

6.

Maintenance ....................................................................

14

 

6.1

General .................................................................

14

 

6.2

Fuses.....................................................................

14

7.

Detailed Description ........................................................

16

 

7.1

General operating principle...................................

16

 

7.2

Front and rear panels............................................

16

 

7.3

Control panel........................................................

16

 

7.4

Description of safety features................................

18

 

7.5

Manual restart in operation mode 8 .....................

20

8.

Technical specification .....................................................

22

9.

Ordering information.......................................................

23

Short instructions (Back page)

 

2

1. Introduction and 2. Safety information

2.1Safety precautions

1. Introduction

MultiTemp® III circulates constant temperature liquid to external electrophoresis equipment. The liquid temperature range is-10to +90 °C. The unit contains KLEA 134a*, an ozone friendlyHFC-typecoolant.

MultiTemp III both supplies coolant for high voltage techniques and provides temperature control at higher temperature (e.g. 60 °C) when performing DNA sequencing. When used with equipment such as the Pharmacia Biotech Multiphor® II electrophoresis unit, two units can be connected to MultiTemp III at the same time.

MultiTemp III can also be used as a stand-aloneconstant temperature water bath.

*KLEA 134a is the Trademark of ICI.

2. Safety information

Read this entire manual before using MultiTemp III.

1.This instrument is designed for indoor use only.

2.The unit must be connected to a grounded mains socket.

3.Ensure that the vents at the sides of the instrument are not covered.

4.Do not use in a dusty atmosphere or close to spraying water.

5.Set the temperature limit cut-offcorrectly.

6.Use only water or water with ethylene glycol in the bath.

3

1.Introduction and 2. Safety information

7.Do not use the circulator beyond its specifications or for other than its intended use.

8.Do not touch the stainless steel bath cover and tubings when the unit is used at a high working temperature. Under these conditions, the surface temperature can rise up to approximately 70 °C.

2.2Safety standards

MultiTemp III is designed in accordance with:

Protection class 1 according to IEC 536: all conductive parts that might become accessible are connected to earth.

Protection IP 32 according to IEC 529/EN 60529: protection against accidentally touching accessible live parts by penetration of particles with a thickness or a diameter of more than 2.5 mm and protection against dropping water onto the unit, inclined at max. 15 degree angle.

This product meets the following EG directives:

72/23/EEC through the harmonised standard;

EN 61010-1,Safety requirements for electrical equipment for measurement, control and laboratory use.

-Part 1: General requirements.

-Part 2: Requirements for laboratory Equipment for heating of material.

Note: the operator has to apply the safety precautions listed in section 2.1 above.

89/336/EEC through the standards;

-EN 50081/Part 1:03.93 Electromagnetic Compatibility emission standard

-EN 50082/Part 1:03.93 Electromagnetic Compatibility, Generic immunity standard.

The CE symbol, and corresponding declaration of confirmity, is valid for the instrument when it is

-used as a “stand alone” unit or

-connected to other CE marked Pharmacia Biotech instruments or

-connected to other products recommended or described in this manual and

-is used in the same state as it was delivered from Pharmacia Biotech AB except for alterations described in this manual.

4

1. Introduction and 2. Safety information

2.3Safety features

General cut-off

The heater, the compressor and the circulation pump can be switched off by a relay built into the unit. This general cutoff will be activated if a malfunction is detected by the safety sensors.

See 7.4, Description of Safety Features.

Protection against over-heating

A built-intemperature limit switch protects against uncontrolled heating of the bath liquid by activating the generalcut-offif this limit is exceeded.

The built-intemperature limit switch also senses low levels of coolant.

See 7.4, Description of Safety Features.

Pump motor protection

When the temperature in the winding of the pump motor reaches a certain level, the general cut-offwill be activated, preventing the motor from overheating.

See 7.4, Description of Safety Features.

Sensor check

The temperature sensor is permanently monitored. The general cut-offis activated if sensor breakage or current leakage is detected.

See 7.4, Description of Safety Features.

Restart after power failure

MultiTemp III automatically restarts after a power failure. See 7.4, Description of Safety Features.

5

3. Installation

3. Installation

3.1Unpacking and installation

1.Check the contents against the packing list supplied. Inspect for any damage that may have occurred during transit. Report any damage immediately to your local Pharmacia Biotech representative and to the transport company concerned.

2.Place MultiTemp III in its working location, ensuring adequate space for ventilation of air through the grids on the sides (air outlet) and rear (air inlet) of the unit.

3.Allow MultiTemp III to stand in an upright position for

24hours before use. Compressor lubricant may have run into the liquifier and evaporator if the unit has not been transported in an upright position.

6.

2.

1.

3.

5.

9.

 

4.

 

8.

8.

7.

10.

Fig. 1. Front and rear view of MultiTemp III.

1.Control Panel

2.Temperature limit dial

3.Outlet nozzle

4.Inlet nozzle

5.Mains cable

6.Circuit breaker

220 V, two thermal fuses

115 V, one thermal fuses

7.Bath Drain

8.Handles (front and rear)

9.Cover lid

10.Air ventilation

6

3. Installation

3.2.Tubing connections

3.3Recommended bath liquids

3.4Filling and draining the bath

Silicone tubing (inner diameter 8 mm) can be used over the entire working temperature range of the unit. PVC tubing can only be used in the temperature range of 10 to 60 °C.

1.Connect the inlet and outlet tubing to the nozzles on the rear of the circulator (Fig. 1).

2.Connect the other ends to the electrophoresis unit. The nozzles have an outer diameter of 9.5 mm. To ensure safe tubing connections, secure all tubing with hose clamps.

When MultiTemp III is used as a water bath, the nozzles should be connected to each other.

Condensation of water on cold tubing can be avoided by covering it with insulating tubing. (See Insulation for cooling tubing in the Ordering Information section.)

Condensation on the cooling plate of Multiphor II during sample application can be avoided by shutting off the circulation to the cooling plate with a three way valve. (See 3-wayValve Set in the Ordering Information section.)

Note: Do not touch or remove tubing when the unit is in operation. Stay alert to the dangers of hot water. The surface temperature of the tubing can reach 70 °C at high working temperatures.

Use distilled water in the temperature range of 5 to 90 °C. A mixture of distilled water and ethylene glycol in a concentration up to 20% is suitable for lower ranges (-10to +40 °C).

Warning! Ethylene glycol is poisonous. Handle with care and follow recommended safety procedures.

Warning! Methanol and ethanol are not recommended because of their low flash points. Both liquids are unsafe to use.

Remove the plastic lid and fill the bath with liquid. The distance between the surface of the liquid and the stainless steel top plate should be about 2 cm. If it is more than 4 cm, a sensor will activate the temperature limit switch and cause cut-off.

To drain the bath, switch off the mains power and remove the outlet tubing from the external equipment and let the

7

3. Installation

3.5Mains connection

liquid pour into a large vessel using hydrostatic pressure. The bath contains 3 litres of liquid, plus the volume in tubing and connected equipment. The last millilitres can be removed by opening the screw on the front of the unit, (see Fig. 1).

Warning! Do not drain hot water. Use protective gloves and clothing when draining the bath liquid.

Compare the local mains voltage and frequency with the specifications written on the label on the rear of the unit before connecting the unit to a grounded mains socket. Deviations of voltage and frequency must not be more than those specified in this manual (95 to 132 V/60 Hz, 95 to 125 V/ 50 Hz, 198 to 253 V/50 Hz). Connect the unit to a grounded mains socket only. The socket must be rated as suitable for the total power consumption of the unit (1,200 W).

8