Leica M50
Leica M60
Leica M80
User Manual
General Instructions
Safety concept
Before using your microscope for the first time, please read the "Safety concept" brochure included with your instrument. It contains additional information about handling and care.
Use in clean rooms
The Leica M series can be used in clean rooms without any problems.
Cleaning
Do not use any unsuitable cleaning agents, chemicals or techniques for cleaning.
Never use chemicals to clean colored surfaces or accessories with rubberized parts. This may damage surfaces and abraded particles may contaminate preparations.
In most cases, we can provide special solutions on request. Some products can be modified, and we can offer other accessories for use in clean rooms.
Servicing
Repairs may only be carried out by Leica Microsystems-trained service technicians. Only original Leica Microsystems spare parts may be used.
Responsibilities of person in charge of instrument
Ensure that the Leica stereomicroscope is operated, maintained and repaired by authorized and trained personnel only.
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Important Safety Notes
User Manual
The individual modules of the Leica M stereomicroscopy series include an interactive CD-ROM with all relevant user manuals in 20 other languages. Keep it in a safe place, and readily accessible to the user. User manuals and updates are also available for you to download and print from our web site at www.stereomicroscopy.com.
This operating manual describes the special functions of the individual modules of the Leica M stereomicroscopy series and contains important instructions for their operational safety, maintenance, and accessories.
The"Safetyconcept"bookletcontainsadditional safety information regarding the service work, requirements and the handling of the stereomicroscope, accessories and electrical accessories as well as general safety instructions .
You can combine individual system articles with articles from external suppliers (e.g. cold light sources, etc.). Please read the user manual and the safety requirements of the supplier.
Before installing, operating or using the instruments, read the user manuals listed above. In particular, please observe all safety regulations.
To maintain the unit in its original condition and to ensure safe operation, the user must follow the instructions and warnings contained in these user manuals.
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Symbols Used
Warning of a danger
•This symbol indicates especially important information that must be read and
complied with.
Failure to comply can cause the following:Hazards to personnel
Functional disturbances or damaged instruments
Warning of hazardous electrical voltage
This symbol indicates especially important information that is mandatory to
read and observe.
Failure to comply can cause the following:Hazards to personnel
Functional disturbances or damaged instruments
Danger due to hot surface.
This symbol warns against touching hot surfaces, e.g. those of light bulbs.
Important information
This symbol indicates additional information or explanations that are intended to
provide clarity.
Explanatory notes
This symbol within the text stands for additional information and explanations.
Figures
(1)Numbers in parentheses within the descriptions relate to the figures and the items within those figures.
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Safety Regulations
Description
The individual modules fulfill the highest requirements for observation and documentation of Leica stereomicroscopes of the M series.
Designated use
Refer to "Safety Concept" booklet
Non-intended use
Refer to "Safety Concept" booklet
Never use M series microscopes or their components for surgical procedures (e.g. on the eye) unless they are specifically intended for that purpose.
The instruments and accessories described in this operating manual have been tested for safety and potential hazards. The responsible Leica affiliate must be consulted whenever the instrument is altered, modified or used in conjunction with non-Leica components that are outside of the scope of this manual.
Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims!
Place of use
Refer to "Safety Concept" booklet
Set up the electrical components at least 10 cm from walls and combustible objects.
Avoid large temperature fluctuations, direct sunlight and vibrations. These conditions can distort measurements and micrographic images.
In warm and warm-damp climatic zones, the individual components require special care in order to prevent the buildup of fungus.
Responsibilities of person in charge of instrument
Refer to "Safety Concept" booklet
Ensure that:
The M series stereomicroscopes and accessories are operated, maintained and repaired by authorized and trained personnel only.
All operators have read, understood and observe this User Manual, and particularly the safety regulations.
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Safety Regulations (cont'd.)
Repairs, service work |
Installation in third-party products |
assignments and work flow (changing tasks |
Refer to "Safety Concept" booklet |
Refer to "Safety Concept" booklet |
frequently), thorough training of the personnel, |
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giving consideration to ergonomic and organi- |
Only original Leica Microsystems spare |
Disposal |
zational aspects. |
parts may be used. |
Refer to "Safety Concept" booklet |
The ergonomic design and construction of the |
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Leica M stereomicroscopy series are intended to |
Before opening the instruments, switch off |
Legal requirements |
reduce the exertion of the user to a minimum. |
the power and unplug the power cable. |
Refer to "Safety Concept" booklet |
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Direct contact with eyepieces can be a potential |
Touching the live circuit can cause injury. |
EC Declaration of Conformity |
transmission path for bacterial and viral infec- |
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Refer to "Safety Concept" booklet |
tions of the eye. |
Transport |
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The risk can be kept to a minimum by using |
Use the original packaging for shipping or |
Health risks |
personal eyepieces for each individual or |
transporting the individual modules of the |
Workplaces equipped with stereomicroscopes |
detachable eyecups. |
Leica M stereomicroscopy series and the |
facilitate and improve the viewing task, but |
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accessory components. |
they also impose high demands on the eyes |
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and holding muscles of the user. Depending |
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In order to prevent damage from vibrations, |
on the duration of uninterrupted work, asthe- |
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disassemble all moving parts that (accord- |
nopia and musculoskeletal problems may |
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ing to the user manual) can be assembled |
occur. For this reason, appropriate measures |
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and disassembled by the customer and |
for reduction of the workload must be taken: |
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pack them separately. |
optimal arrangement of workplace, work |
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Safety Regulations (cont'd.)
Light sources: safety regulations
Protective measures of the manufacturer:
UV protection screen in front of the specimen plane prevents the user from looking directly into the UV rays.
Dummy filter carriers in the free positions of the rapid filter changer prevent direct UV radiation from reaching the eyes.
UV filters are installed in the observation beam paths to protect the eyes.
The stray-light protection on the lamp housing prevents irradiation of the hands.
Warning
UV radiation could damage the eyes. Therefore:
Never look into the light spot on the specimen plane without a UV protection screen.
Never look into the eyepieces if no excitation filter is in the beam path.
Fill empty filter positions with dummy filter carriers (e.g. M165 FC).
Do not select a white, strongly reflective background for the specimen.
Supply unit
Always unplug the supply unit from the power supply:
When installing and disassembling the lamp housing
Before opening the lamp housing
When replacing the high-pressure mercury lamp and other parts, such as the heatabsorbing filter or the collector
During maintenance work on the supply unit
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Safety Regulations (cont'd.)
Lamp housing
Never open the lamp housing while the lamp is switched on. Risk of explosion, UV exposure, blinding!
Before opening the lamp housing, allow it to cool off for at least 15 minutes. Danger of explosion!
Never cover the air duct on the lamp housing. Danger of fire!
Mercury lamp
Follow the user manual and safety regulations provided by the lamp manufacturer, and, in particular, the section on how to proceed if a lamp breaks and releases mercury.
For transport, remove the mercury lamp, transport it in its original packaging and protect moving parts in the lamp housing using the transport anchors.
When it has reached the end of its rated life (follow the manufacturer's specifications and the minute meter on the supply unit).
To minimize the risk of explosion, replace a discolored mercury lamp promptly.
Leica Microsystems assumes no liability for damage caused by exploding, incorrectly installed or improperly used mercury lamps.
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Table of Contents
Inhaltsverzeichnis
General Instructions |
2 |
Important Safety Notes |
3 |
Symbols Used |
4 |
Safety Regulations |
5 |
Safety Regulations (cont'd.) |
6 |
Safety Regulations (cont'd.) |
7 |
Safety Regulations (cont'd.) |
8 |
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M series |
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Congratulations! |
12 |
The Modular Design: Everything is Relative |
13 |
Maximum Compatibility |
14 |
On We Go |
15 |
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Assembly |
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Base and Focusing Column |
17 |
Microscope Carrier |
18 |
Optics Carrier |
19 |
Tube |
20 |
Eyepieces |
21 |
Objective |
22 |
Leica LED5000 CXI (Coax Illumination) |
23 |
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Leica LED5000 CXI (cont'd.) |
24 |
Leica LED3000 NVI™ (Near Vertical Illumination) |
25 |
Leica LED3000 NVI™ (cont'd.) |
26 |
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Quick Start Guide |
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The Reliable Route to Success |
28 |
Overview of an M Series Microscope |
29 |
The Correct Interpupillary Distance |
30 |
Using the Eyepieces |
31 |
Focusing |
32 |
Adjusting the Resistance of the Focus Drive |
33 |
Changing the Magnification (Zoom) |
34 |
Ratchet Steps and Magnification Levels |
35 |
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Eyepieces & Tubes |
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Magnification Factor of the Eyepieces |
37 |
Health Notes |
38 |
Dioptric Correction |
39 |
Dioptric Correction and Parfocality |
40 |
Graticules |
41 |
ErgoTube 10° to 50° |
42 |
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Photography & Video |
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Photography & Video |
44 |
Photo Tubes and C-mounts |
45 |
Video/Phototubes HD F & HD V |
46 |
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Optical Accessories |
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The Different Types of Objectives |
48 |
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System Illumination |
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Leica LED3000 NVI™ (Near Vertical Illumination) |
50 |
Leica LED5000 CXI (Coax Illumination) |
51 |
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Dimensional Drawings |
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Leica M50 |
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Leica M50 |
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Leica M60 |
56 |
Leica M80 |
57 |
Leica M80 |
58 |
Leica M80 |
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Leica M80 |
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Leica M80 |
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Appendix |
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Calculating the Total Magnification and Field of View Diameter |
63 |
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Care, Maintenance, Contact Persons |
64 |
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Care, Maintenance, Contact Persons (cont'd.) |
65 |
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The Leica M series
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Congratulations!
The New Leica M Series
The introduction of the Leica M50, M60 and M80 adds three new highquality routine stereomicroscopes to the proven M-Series range from Leica Microsystems. The optical brilliance and the wide range of accessories make them the ideal microscope for quality assurance and similar industrial applications.
Magnification in stages or zoom
The Leica M50 stereomicroscope includes precisely reproducible magnification levels for repeated examinations, measurements, drawing or photography of objects under absolutely identical conditions. The five easily selectable positions can be set without removing the eyes from the eyepieces. This ensures that your results remain comparable at all times without great efforts.
The Leica M60 and M80 stereomicroscopes can be used for a wide range of routine applications with the 6:1 / 8:1 zoom and switchable ratchet steps. The large working distance and the brilliant imaging power shows you the finest details of your specimens without losing your overview of large workpieces.
Common to all microscopes is the Leica range of accessories. Whether you need a variety of illumination types, a wide selection of objectives or the Leica swinging-arm system – there is a solution for everything there!
Do you already own stereomicroscopic equipment and are thinking of switching to Leica? No problem! Leica M50, M60 and M80 fit into microscope carrier with a diameter of 76 mm and are therefore compatible with many suppliers. You can simply continue to use your components and add the proven Leica stereomicroscopes.
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The Modular Design: Everything is Relative
The Leica M series provides maximum flexibility in choosing equipment, thanks primarily to the modular configuration and the compatibility that Leica has painstakingly maintained for decades. The optics carriers, eyepieces, bases and more can be combined in any way you choose, allowing you to create the microscope that best suits your needs.
Despite this, you will notice that the controls and individual components do not differ significantly. Whichever configuration you choose, you will quickly feel right at home.
Have a special request? Let us know!
Leica Microsystems enjoys an exceptional reputation when it comes to devising customer-
specific solutions. If you have a special request that cannot be met with standard parts, contact your Leica consultant. We have a solution for every problem.
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Maximum Compatibility
Leica engineers were careful to ensure that the new Leica M series—like its predecessors— remains compatible with existing series. This means that objectives, bases, tubes and so on can be reused.
Objectives
All objectives of this new generation have the same reference focal length as the objectives of the MZ generation. If you prefer, you can therefore continue to use the previous Leica objective series.
Tubes
The interface between the optics carrier and the tube has remained the same, so existing tubes fit the new M series. The new tubes are designed for eyepieces with field number 23, while the predecessor models were only designed for field number 21, resulting in a smaller object field.
Eyepieces
The new M-series eyepieces have an audible and tangible click to provide immediate feedback in case of accidental adjustment.
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On We Go
If your new Leica microscope has already been assembled and commissioned by your Leica consultant, click here to skip through the installation instructions and go directly to the Quick Start Guide on Page 29.
If, on the other hand, you are assembling the microscope yourself, continue with the "Assembly" chapter, which begins on Page 16.
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Assembly
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Base and Focusing Column
The first step is to connect the focusing column |
Assembling the column adapter |
2. Install the base on the column using the |
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of the M series to the corresponding base. |
1. Remove the three screws provided from the |
three screws. |
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column. |
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Tools used |
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Hex socket screwdriver, 3 mm |
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Tip: Place the column on the edge of a table to attach the base and have another person help you.
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Microscope Carrier
Tools |
Alternative installation options |
Allen screwdriver provided |
Depending on the desired working distance, the |
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microscope carrier can be installed in the stan- |
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dard way or upside down (see figure below). |
Assembly |
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The microscope carrier is fastened to the column |
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using the screw provided. |
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Optics Carrier
Tools used |
Assembling the optics carrier |
None |
1. Unscrew the clamping screw on the holder |
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for the optics carrier. |
2. Place the optics carrier into the holder and align it in such a way that the notch in the optics carrier and the bottom clamping screw are on top of each other.
2.Tighten the clamping screw below the optics carrier.
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Tube
All intermediate tubes that fit between the optics carrier and the binocular tube are fitted in the same manner.
Tools used
No tools required.
Preparation
1.Unscrew the positioning screw and remove the protective cover.
Assembling the tube
2.Push the tube (for example, the inclined binocular tube) into the dovetail ring and rotate it slightly in both directions until the positioning screw meshes with the guide groove.
3. While holding the tube only slightly, carefully tighten the positioning screw. It is automatically brought to the correct position .
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