Leica DM5000B, DM4000M User Manual

4.3 (4)

Leica DM4000B

Leica DM4000M

Leica DM4500P

Leica DM5000B

Operating Manual

Bedienungsanleitung

1

Published September 2007 by:

Herausgegeben September 2007 von:

Leica Microsystems CMS GmbH

Ernst-Leitz-Straße

D-35578 Wetzlar (Germany)

Responsible for contents:

 

 

Verantwortlich für den Inhalt:

 

 

Dr. Jasna Roeth, Stefan Motyka

 

 

(Marketing CM, Compound Microscopy, Product Management)

 

 

(Marketing CM, Compound Microscopy, Produktmanagement)

 

 

Holger Grasse

 

 

(Safety Officer according to MPG §30)

 

 

(Sicherheitsbeauftragter nach MPG §30)

 

 

In case of questions, please contact the hotline:

 

 

Bei Fragen wenden Sie sich bitte an die Hotline:

Phone

+49 (0) 64 41-29 22 86

 

Fax

+49 (0) 64 41-29 22 55

E-Mail: MQM-Hotline@leica-microsystems.com

2

Leica DM4000B

Leica DM4000M

Leica DM4500P

Leica DM5000B

Operating Manual

3

Copyrights

Copyrights

All rights to this documentation are held by Leica Microsystems CMS GmbH. Reproduction of text or illustrations (in whole or in part) by print, photocopy, microfilm or other method (including electronic systems) is not allowed without express written permission from Leica Microsystems CMS GmbH.

The term "Windows" may appear in the following text without further identification. It is, however, a registered trademark of Microsoft Corporation. The names of companies and products used herein may be trademarks of their respective owners.

The instructions contained in the following documentation reflect state-of-the-art technology and knowledge standards. We have compiled the texts and illustrations as accurately as possible. Nevertheless, no liability of any kind may be assumed for the accuracy of this manual’s contents. Still, we are always grateful for comments and suggestions regarding potential mistakes within this documentation.

The information in this manual is subject to modification at any time and without notification.

4

 

 

 

 

 

 

 

Contents

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Contents

 

 

 

 

 

 

 

1.

Important notes about this manual .......

7

7.

Startup .........................................................

35

 

 

 

7.1

Functional principle ..................................

35

2.

Intended purpose of the microscope ....

8

7.2

Switching on the unit ................................

38

 

 

 

7.3

The display

 

3.

Safety notes ................................................

9

 

(DM4000 B/4500 B/4000 M/4500 P) .........

39

3.1

General safety notes .................................

9

7.4

The function keys ......................................

40

3.2

Electrical safety .........................................

10

7.5

Köhler illumination ....................................

41

3.3

Disposal .......................................................

11

 

7.5.1

Transmitted light .............................

41

 

 

 

 

7.5.2

Incident light ....................................

42

4.

Overview of the instrument ....................

12

7.6.

Checking the phase contrast rings ........

44

 

 

 

7.7

Setting the motorized polarizer

 

5.

Unpacking the microscope .....................

17

 

(DM4500 P/DM5000 B) ..............................

45

 

 

 

7.8

Adjusting the light sources ......................

45

6.

Assembling the microscope ...................

19

 

 

 

 

 

 

6.1

Specimen stage .........................................

19

8.

Operation ....................................................

51

6.2

Condenser ...................................................

21

8.1

Switching on the unit ................................

51

6.3

Tube and eyepieces ..................................

22

8.2

Stages and object displacement ............

51

6.4

Objectives ...................................................

23

8.3

Focusing ......................................................

53

6.5

Light sources for the transmitted

 

8.4

Tubes

.......................................................

 

 

53

 

light axis ......................................................

23

8.5

Eyepieces ....................................................

55

6.6

Light sources for the incident

 

8.6

Objectives ...................................................

55

 

light axis ......................................................

25

8.7

Magnification changer .............................

58

6.7

Equipping the incident light

 

8.8

HC P 1x/1.6x tube optics ...........................

58

 

turret disk ....................................................

30

8.9

Light sources ..............................................

59

6.8

Polarizer and analyzer ..............................

31

8.10

Aperture diaphragm and

 

6.9

DIC prisms ...................................................

32

 

field diaphragm ..........................................

59

6.10

Optional accessories ................................

33

 

 

 

 

 

 

6.11

Connection to the power supply ............

34

 

 

 

 

 

 

6.12

Connecting to the CTR5000

 

 

 

 

 

 

 

 

electronics box ..........................................

34

 

 

 

 

 

 

5

Contents

9.

Contrast methods for

 

11.

 

Troubleshooting .........................................

74

 

Leica DM4000 B/DM4500 B/

 

 

 

 

 

 

DM4500 P/DM5000 B ................................

60

12.

 

Care of the microscope ...........................

77

9.1

Transmitted light ........................................

60

12.1

Dust cover ...................................................

77

 

9.1.1 Bright field .........................................

60

12.2

Cleaning .......................................................

77

 

9.1.2 Phase contrast ..................................

60

12.3

Handling acids and bases .......................

78

 

9.1.3 Dark field ............................................

61

 

 

 

 

 

9.1.4 Polarization ........................................

61

13.

 

Essential wear and spare parts .............

79

 

9.1.4.1 Manual method .............................

61

 

 

 

 

 

9.1.4.2 DM4500 P - examinations

 

14.

 

Abbreviations and pictograms ...............

80

 

in polarized transmitted light ......

62

 

 

 

 

 

9.1.4.3 Motorized method ........................

68

15.

 

Index ............................................................

81

 

9.1.4.4 Combined methods .......................

68

 

 

 

 

 

9.1.5 Differential interference

 

16.

EU Declaration of Conformity .................

82

 

contrast ............................................

68

 

 

 

 

 

9.1.5.1 DM4500 B/DM4500 P ....................

68

 

 

 

 

 

9.1.5.2 DM5000 B ........................................

69

 

 

 

 

9.2

Fluorescence ..............................................

70

 

 

 

 

10.

Contrast methods for

 

 

 

 

 

 

Leica DM4000 M ........................................

71

 

 

 

 

10.1

Incident light ..............................................

71

 

 

 

 

 

10.1.1 Bright field .......................................

71

 

 

 

 

 

10.1.2 Dark field ..........................................

71

 

 

 

 

 

10.1.3 Polarization ......................................

72

 

 

 

 

 

10.1.4 Interference contrast ....................

73

 

 

 

 

10.2

Transmitted light ........................................

73

 

 

 

 

 

10.2.1 Bright field .......................................

73

 

 

 

 

 

10.2.2 Polarization ......................................

73

 

 

 

 

6

1. Important notes about this manual

1. Important notes about this manual

Caution!

This operating manual is an essential component of the microscope, and must be read carefully before the microscope is assembled and put into operation.

Text symbols, pictograms and their meanings:

(1.2)

p.20

!

*

This operating manual contains important instructions and information for the operational safety and maintenance of the microscope and accessories. It must therefore be kept safely for future reference.

Numbers in parentheses, such as "(1.2)", correspond to illustrations (in the example, Figure 1, Item 2).

Numbers with pointer arrows (for example → p.20), point to a certain page of this manual.

Caution!

Special safety instructions within this manual are indicated with the triangle symbol shown here, and have a gray background.

Caution! The microscope and accessories can be damaged when operated incorrectly.

Explanatory note.

Instructions on disposing of the microscope, accessory components and consumables.

Item not contained in all configurations.

7

2. Intended purpose of the microscope

2. Intended purpose of the microscope

The DM4000 – DM5000 microscopes to which these operating instructions belong, and which have the identifying letter B, are intended for biological routine and research applications. This includes examining specimens taken from the human body for the purpose of gaining information about physiological or pathological conditions or inborn anomalies, or testing for safety and compatibility for potential recipients, or for monitoring therapeutic measures.

The microscopes that have the identifying letters M or P are intended for materials science, geological or mineralogical examinations.

The above-named microscopes comply with the Council Directive 98/79/EEC concerning in vitro diagnostics. They also conform to the Council Directives 73/23/EEC concerning electrical apparatus and 89/336/EEC concerning electromagnetic compatibility for use in an industrial environment.

Caution!

The manufacturer assumes no liability for damage caused by, or any risks arising from using the microscopes for other purposes than those for which they are intended or not using them within the specifications of Leica Microsystems CMS GmbH.

In such cases the declaration of conformity shall cease to be valid.

Caution!

These (IVD) instruments are not intended for use in the patient environment defined by DIN VDE 0100-710. Nor are they designed to be combined with medical instruments in accordance with EN 60601-1. If a microscope is electrically connected to a medical instrument in accordance with EN 60601-1, the requirements defined in EN 60601-1-1 shall apply.

8

3. Safety notes

3. Safety notes

3.1 General safety notes

This safety class 1 device was built and tested in accordance with the safety regulations for electrical measuring, control, regulating and laboratory devices in accordance with

EN 61010-2-101:2002 EN 61010-1:2001 IEC 1010-1:2001

Caution!

In order to maintain this condition and to ensure safe operation, the user must follow the instructions and warnings contained in this operating manual.

Caution!

The devices and accessories described in this operating manual have been tested for safety and potential hazards.

The responsible Leica affiliate or the main plant in Wetzlar, Germany, must be consulted whenever the device is altered, modified or used in conjunction with non-Leica components that are outside of the scope of this manual.

Unauthorized alterations to the device or noncompliant use shall void all rights to any warranty claims and void product liability!

9

3. Safety notes

3.2 Electrical safety General specifications

Leica CTR5000 electronics box (for DM5000 B)

For indoor use only. Supply voltage: Frequency:

Power input: Fuses:

Ambient temperature: Relative humidity: Overvoltage category: Pollution degree:

Microscope

For indoor use only. Supply voltage: Frequency:

Power input: DM4000 DM4500 DM5000

Fuses:

DM4000

DM4500

DM5000

Ambient temperature: Relative humidity: Overvoltage category: Pollution degree:

90-250 V~

50-60 Hz max. 290VA T6.3 A

(IEC 60127-2/3) 15-35°C

max. 80% to 30°C II

2

90-250 V~

50-60 Hz

max. 180 VA max. 180 VA max. 290VA

T6.3 A (IEC 60127-2/3)

T6.3 A (IEC 60127-2/3) See CTR5000 15-35°C

max. 80% to 30°C II

2

ebq 100 supply unit*

 

For indoor use only.

 

Supply voltage:

90-250 V~

Frequency:

50-60 Hz

Power input:

max. 155VA

Fuses:

2xT2A (IEC 127)

Ambient temperature:

10-36°C

Relative humidity:

max. 80% to 30°C

Overvoltage category:

II

Pollution degree:

2

(see enclosed manual)

 

Caution!

The power plug may only be plugged into an outlet equipped with a grounding contact.

Do not interfere with the grounding function by using an extension cord without a ground wire. Any interruption of the ground wire inside or outside of the device, or release of the ground wire connection, can cause the device to become hazardous. Intentional ground interruption is not permitted!

Caution!

Through connection to the grounding connection, ancillary equipment with its own and/or extra power supply may be brought to the same ground wire potential. For connections without a ground connector, Leica Service must be consulted.

10

3. Safety notes

Caution!

Never use any fuses as replacements other than those of the types and the current ratings listed here. Bypassing fuse holders is not permitted.

Caution!

The microscope’s electrical accessory components are not protected against water. Water can cause electric shock.

Caution!

Protect the microscope from excessive temperature fluctuations. Such fluctuations can lead to the accumulation of condensation, which can damage the electrical and optical components.

Operating temperature: 15-35°C

3.3 Disposal

Once the product has reached the end of its service life, please contact Leica Service or Sales about disposal.

Please observe and comply with the national and federal laws and regulations that are equivalent to EU guidelines such as WEEE.

Note!

Like all electronic devices, the microscope, its accessory components and consumables must never be disposed of with general household waste.

Caution!

Before exchanging the fuses or lamps, be absolutely certain to switch off the main power switch and remove the power cable.

11

4. Overview of the instrument

4. Overview of the instrument

Specification

Leica DM4000 B

Leica DM4000 M

 

Leica DM5000 B

Leica DM4500 P

 

 

 

 

 

Contrast methods

Transmitted light:

Transmitted light:

 

 

DM4000 B: BF, DF, PH, Pol

 

DM4000 M: BF, DF, PH, ICT,

 

 

DM5000 B: and ICT (mot.)

 

Pol

 

Incident light: Fluorescent

 

DM4500 P: BF, DF, PH, ICT,

 

 

 

 

 

Pol (conoscopy)

 

 

 

 

Incident light:

 

 

 

 

 

BF, DF, ICR, Pol, Fluo

 

 

 

 

Transmitted light axis

 

• Automatic illumination manager (mot. aperture diaphragm

 

 

 

and field diaphragm, mot. intensity control)

 

 

Automatic constant-color intensity control

 

 

Motorized shutter

 

 

 

 

 

 

Incident light axis

Integrated into the stand

• Integrated into the stand

 

• Motorized 5x filter turret disk

• Motorized 4x filter turret

 

 

(DM5000 B 8x optional)

 

disk

 

• With

FIM (fluorescence in-

Automatic

 

 

tensity manager) for reduc-

 

illumination manager

 

 

ing the light intensity in 5 in-

DM4000 M: motorized

 

 

crements

 

shutter

 

Mechanical booster lens for

 

 

 

 

increasing fluorescence in-

 

 

 

 

tensity

 

 

 

Motorized shutter

 

 

 

 

 

 

 

 

Z pinion

 

Manual

 

 

 

 

 

 

 

Objective turret

Manual, fully encoded

Manual, fully encoded

 

• DM4000 B: 6x/7x with

• DM4000 M: 6x with

 

 

 

M25 thread

 

M32 thread

 

 

DM5000 B: 7x (M25)

 

DM4500 P: 6x with

 

 

DM5000 B: With object prism

 

M25 thread, centerable,

 

 

disk (3 positions)

 

encoded

 

 

 

 

Receptacle for DIC prisms

 

 

 

 

 

and Pol compensators

 

 

 

 

 

(for DM4000 M: optional)

 

 

 

 

 

 

12

4. Overview of the instrument

Specification

Leica DM4000 B

 

Leica DM4000 M

 

Leica DM5000 B

 

Leica DM4500 P

 

 

 

 

 

 

X/Y stage

Manual

 

Manual

 

Replaceable specimen stage

 

DM4000 M:

 

Coaxial drive length: 155 mm

 

 

• Replaceable specimen stage

 

 

 

 

 

 

 

• Coaxial drive length: 140 mm

 

 

 

 

 

 

 

DM4500 P:

 

 

 

 

 

 

 

• Replaceable Pol stage

 

 

 

 

 

Tube

 

Manual or motorized (DM4500P: manual)

 

 

Optionally with one or two camera outputs

 

 

DM4500 P: conoscopy module

 

 

 

 

(tube optics HC P1x/1.6x with Bertrand lens, encoded)

 

 

 

 

 

 

 

Condenser

 

Motorized condenser head

 

 

 

 

Condenser disk for the light ring, DF-Stop, DIC prisms

 

 

Automatic Köhler illumination

 

 

 

Optional polarizer (integrated and motorized)

 

 

 

 

 

 

Magnification changer

Manual

 

Manual

(optional)

3x fully encoded

 

3x fully encoded

 

1x; 1.25x; 1.6x

 

1x; 1.5x; 2x

 

 

 

 

 

Controls

 

Operating buttons on the stand for all motorized

 

 

 

microscope functions

 

 

 

 

Additional variable multifunction keys

 

 

Focus wheels

 

 

 

 

LCD

 

 

 

 

DM5000 B with Leica SmartTouch

 

 

 

 

 

 

 

Computer interface

 

USB2.0

 

 

 

 

 

 

 

 

Software tools

 

Leica Application Suite (LAS)

 

 

 

 

for WindowsTM 2000, XP, Vista

 

 

 

• With plug-ins for:

 

 

 

 

 

Microscope and camera configuration

 

 

 

Microscope and camera control

 

 

 

Image acquisition

 

 

 

 

 

 

 

 

 

 

13

4. Overview of the instrument

Specification

Leica DM4000 B

Leica DM4000 M

 

Leica DM5000 B

Leica DM4500 P

 

 

 

Electronics box

Only for the Leica DM5000 B:

 

Leica CTR5000

Separate operating unit with a

 

 

power supply for 100 W halogen

 

 

lamps. See → p.10

 

 

(Electrical safety)

 

 

 

 

14

4. Overview of the instrument

1

2

3

14

4

5

6

7

13

12

11

10 9

8

 

 

Fig. 1

Left side of the stand with the advanced AET22 ErgoTube

 

1

Eyepiece

8

Field diaphragm operating buttons

2

Eyepiece tube

9

Transmitted light / incident light switch

3

Tube

 

10

Aperture diaphragm operating buttons

4

Objective turret with objectives

11

Brightness adjustment buttons

5

Specimen stage with specimen holder

12

Focus wheel with coarse and fine adjustment

6

Condenser

13

Variable function keys (preset at the factory)

7

LCD

 

14

Lamp adjustment window

15

4. Overview of the instrument

15

22

16

21

20

19 18

17

Fig. 2 Right side of the stand with the advanced ErgoTube AET22

15Lamp housing for incident light

16Lamp housing for transmitted light

17Transmitted light filter, optional

18Transmitted light filter, optional

19Variable function keys (preset at the factory)

20x/y coaxial drive, adjustable height

21Handwheel for fine focus

22Motorized filter block exchanger

16

5. Unpacking the microscope

5. Unpacking the microscope

The microscope is delivered in two packages.

The stand package contains the following components:

Stand with integrated incident light axis and objective turret

Specimen stage with stage bracket

Power cable and PC connecting cable

CD with Leica Application Suite (LAS) software package

Instructions and list of microscope default settings

The system package contains the microscope’s accessories:

Tube

Eyepieces

Objectives

Condenser

Lamp housings with accessories

Assembly tools

Additional microscope accessories such as filter cubes, etc. depending on product configuration

The external ebq 100 supply unit* is delivered in a separate package.

For the Leica DM5000 B microscope:

The Leica CTR5000 electronics box is delivered in a separate package.

First, carefully remove all components from the transportation and packaging materials.

Note:

If at all possible, avoid touching the lens surfaces of the objectives. If fingerprints do appear on the glass surfaces, remove them with a soft leather or linen cloth. Even small traces of finger perspiration can damage the surfaces of optical devices in a short time. See the chapter on "Care of the microscope" → p. 77 for additional instructions.

Caution!

Do not connect the microscope or peripherals to an AC power source at this time under any circumstances!

17

5. Unpacking the microscope

Installation location

Work with the microscope should be performed in a dust-free room, which is free of oil vapors and other chemical vapors, as well as extreme humidity. At the workstation, large temperature fluctuations, direct sunlight and vibrations should be avoided. These conditions can distort measurements and micrographic images.

Allowable ambient conditions

Temperature

15-35°C

Relative humidity

maximum 80% up to 30°C

Microscopes in warm and warm-damp climatic zones require special care in order to prevent the build up of fungus.

See the chapter on "Care of the microscope" → p. 77 for additional instructions.

Caution!

Electrical components must be assembled at least 10 cm away from the wall and from flammable substances.

Transport

For shipping or transporting the microscope and its accessory components, the original packaging should be used.

As a precaution to prevent damage from vibrations, the following components should be disassembled and packaged separately:

Unscrew the objectives.

Remove the condenser.

Remove the specimen stage.

Remove the lamp housings.

Disassemble the burner of 106 z lamp housing.

Remove all moving or loose parts.

18

6. Assembly

6. Assembling the microscope

The microscope components are logically as- 6.1 Specimen stage sembled in this order:

Specimen stage

Condenser with condenser head

Tube

Eyepieces

Objectives

Lamp housings with light sources

Equipment for the incident light turret disk*

Only a few commonly used screwdrivers and keys are necessary for assembly; these are included in the delivery package.

When using intermediate systems and optical accessories, the sequence may vary.

In this case, read chapter,

"6.10 Optional accessories" → p.33

!Caution:

Never install objectives before assembling the stage.

Place the specimen holder on the stage and fasten it with the two screws (3.1).

Using the condenser height adjuster (3.2), turn the condenser holder completely upwards, i.e. as close to the stage as possible.

Loosen the stage clamp (3.3) slightly.

Fig. 3 Mechanical object stage

1Locking screws for specimen holder

2Condenser height adjuster

3Stage clamp

1

2 3

19

6. Assembly

From above, set the stage clamp onto the dovetail guide (4.2) and push the stage downwards until the upper end of the dovetail guide is tightly fastened to the upper end of the stage clamp.

Firmly tighten the stage clamp (4.1).

Note:

Only for DM4500 P:

Pol attachable mechanical stage*

Adjust the attachable mechanical stage so that the fastening screw is visible below the holes (4a.1). Set the attachable mechanical stage in the guide holes on the rotating stage and tighten the fastening screw using the hexagonal key.

Attachable mechanical stage*

For thicker specimens (Leica DM4000 M) the stage can be set to a correspondingly lower level.

The attachable mechanical stage can be installed on the left, on the right or on the front (not pictured). The two clamping screws fasten it into place.

 

 

 

Fig. 4a Pol rotating stage* and Pol 3 attachable mechani-

 

 

 

cal stage*

 

 

 

1

Holes for the fastening screw.

 

 

 

2

Lever for the holder for glass slides of various formats,

 

 

 

 

which can be turned inward and outward

 

 

 

3

Storage for the centering key

Fig. 4

Assembling the stage

4

Locking button pair

1

Stage clamp

5

45° click stop

2

Dovetail guide

6

Clamping system for the stage rotation

4

 

 

 

3

 

 

 

 

 

 

1

1

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

2

 

 

 

 

 

 

 

 

2

 

 

 

 

 

 

 

5

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

6

 

 

 

 

20

Leica DM5000B, DM4000M User Manual

6. Assembly

6.2 Condenser

Screw the condenser head into the condenser.

Using the condenser height adjuster (5.4), turn the condenser holder (5.1) downward as far as it will go.

Unscrew the clamping screw for the condenser (5.3) far enough so that the condenser can be inserted from the front.

From the front, insert the condenser into the condenser holder as far as it will go. On the underside of the condenser, there is an orientation pin (6.1) that must be locked into place in the guiding notch (7.1).

Tighten the condenser’s clamping screw (5.3) until the condenser locks into place.

Connect the condenser over the connection (8.1) with the stand.

Fig. 5 Condenser holder

1Condenser holder

2Condenser centering

3Clamping screw for the condenser

4Condenser height adjuster

Note:

The condenser must be centered before using the microscope.

→ Köhler illumination p. 41.

Fig. 6

 

Underside of condenser

1

1 Orientation pin

Fig. 7 Condenser holder 1 Guiding groove

1

Fig. 8 Condenser connection 1 Condenser cable socket

1

2 3

4

1

 

21

6. Assembly

6.3 Tube and eyepieces

The tube is mounted on the stand either directly or with the use of intermediate modules. The side clamping screw fastens it into place (9b.1).

For the MBDT motorized tube only:

Remove the transport anchor (9a.1) on the underside of the tube.

Partially unscrew the clamping screw (9b.1).

Insert the tube into the circular receptacle (dovetail ring).

Retighten the clamping screw (9b.1).

For the MBDT motorized tube only:

Connect the tube to the stand with the connector bushing (10.1).

The eyepieces are inserted into the eyepiece tubes on the tube.

For the BDTP tube only:

The Pol eyepieces are inserted into the eyepiece tubes (using the locking groove).

Fig. 9b Fastening the tube 1 Clamping screw

1

Fig. 9a Underside of the tube

Fig. 10 Motorized tube connection

1 Transport anchor

1 Connector socket

1

1

22

6. Assembly

6.4 Objectives

The receptacles on the objective turrets are numbered (Fig. 11). Based on your equipment, the individual objectives have already been assigned to specific positions at the factory.

For details on the exact positions of the objectives, please refer to the enclosed identification sheet.

!Caution:

Close vacant threads in the turret with dust protection caps!

6.5 Light sources for the transmitted light axis

Caution!

Ensure that the lamp housing has been disconnected from the power supply. Unplug the power plug and the power supply during assembly.

Caution!

Light sources pose a potential irradiation risk (glare, UV-radiation, IR-radiation). Therefore, lamps have to be operated in closed housings.

Lamp housing 107/2

This lamp housing is used with a 12 V 100 W halogen lamp, which is already mounted.

In case the lamp has to be removed:

Fig. 11

Objective turret with engraved objective receptacles

• Remove the fastener screw on the housing (Fig. 12).

• Remove the housing by pulling it upwards.

• Remove the lamp.

23

6. Assembly

Insert the new 12 V 100 W lamp (13.1) with the dust cover straight into the socket until it stops. Be sure that the lamp is inserted straight.

Remove the lamp’s dust cover.

Caution!

Do not remove the lamp’s dust cover until after you have installed the lamp. Avoid fingerprints on the lamp.

Replace the housing and fasten it in place using the fastening screw.

Place the lamp housing in the transmitted light lamp housing receptacle (14.2) and fasten it with the clamping screw on the side.

Connect the lamp housing to the power supply for transmitted light (symbol: ) (14.3).

Fig. 12

Lamp housing 107/2 Releasing the fastening screw

Fig. 14 Rear view of the stand

1 Incident light lamp housing receptacle

2 Transmitted light lamp housing receptacle

3 12 V 100 W connection for transmitted light (symbol: ) 4 12 V 100 W connection for incident light (symbol: )

Fig. 13

1

 

 

 

Lamp housing 107/2

 

 

 

opened

 

 

 

 

1

Mount with

1

 

 

 

 

 

 

 

halogen bulb

 

 

 

 

2

Collector

 

 

 

 

 

 

2

 

 

 

 

 

2

 

 

3 4

 

 

 

24

6. Assembly

6.6 Light sources for the incident light axis

Caution!

Light sources pose a potential irradiation risk (glare, UV-radiation, IR-radiation). Therefore, lamps have to be operated in closed housings.

Ensure that the lamp housing has been disconnected from the power supply. Unplug the power plug and the power supply during assembly.

During assembly work on xenon burners, always wear the protective gloves and face protection supplied (Fig. 15) (risk of explosion).

Never touch the glass parts of the burner with bare hands.

Never look directly into the beam path (blinding hazard).

Lamp housing 106/106 z

This lamp housing is suitable for use with a 12 V 100 W halogen lamp or a variety of gas discharge lamps.

Caution!

Make sure to follow the instructions and safety notes of the lamp supplier.

Before changing lamps allow it to cool down for at least 30 min.!

Fig. 16 106/106 z lamp housing (on the side, open)

1Cover raised

2Collector

312 V 100 W lamp or

gas discharge lamp in mount

4Reflector (mirror)

5, 6, 7 Adjusting screw for x-y reflector

8Fastening screw for the lamp mount

9Socket for contact plug

Fig. 15

1

Protective gloves and mask

2

4

5

3

6

7

8

9

8

25

6. Assembly

Inserting the 12 V 100W halogen bulb into the 106/106 z lamp housing

Unscrew the fastening screws of the cover and flip the cover up (16.1).

Unscrew the fastening screws of the lamp mount (16.8) and pull out the mount (Fig. 17).

Insert the lamp with the dust cover straight into the socket until it stops.

Caution!

Do not remove the lamp’s dust cover until after you have installed the lamp. Avoid fingerprints on the lamp.

Insert the lamp mount, with the burner installed, into the lamp housing and tighten it with the screws (16.8).

Close the lamp housing and retighten the fastening screws.

Place the lamp housing in the incident light lamp housing receptacle (18.1) and fasten it with the clamping screw on the side.

Connect the lamp housing to the power supply for incident light (symbol: ) (18.4).

• Remove the dust cover.

 

Fig. 18 Rear view of stand

 

 

1

Incident light lamp housing receptacle

 

 

2

Transmitted light lamp housing receptacle

 

 

3

12 V 100 W connection for transmitted light (symbol:

)

Fig. 17 Lamp mount with 12 V 100 W halogen bulb

4

12 V 100 W connection for incident light (symbol:

)

1

2

 

3 4

 

26

6. Assembly

Inserting gas discharge lamps (Hg and Xe) into the 106/106z lamp housing

Hg and Xe lamps are powered by separate supply units.

Please also read the separate instruction manual provided with these supply units.

The following gas discharge lamps may be used and require different power supplies and lamp mounts (Fig. 19):

Type

Typical bulb life*

 

 

 

50

W high-pressure mercury burner (alternating current)

100 hrs.

100

W high-pressure mercury burner (direct current)

200 hrs.

100

W high-pressure mercury burner (direct current, type 103 W/2)

300 hrs.

75

W high-pressure xenon burner (direct current)

400 hrs.

* Please observe the data sheets of the lamp manufacturer.

27

6. Assembly

To open the 106 z lamp housing, unscrew the fastening screws on the cover.

Remove the transport anchor (red plastic rod in place of the burner) in the lamp mount. To do so, remove the lower clamp (19.1). Pull up the cooling element (19.3) and turn it to the side. Detach the lower clamp system (19.2) and remove the transport anchor.

Install the burner in reverse order.

Caution!

Hg 50 burner:

After installation, the labeling must be upright.

If a glass melt nipple is present (19a.4), position it by turning the burner so that the nipple does not impede the beam path later, but instead is positioned sideways.

Xe 75 burner:

Remove the burner’s dust cover (19b.5) after you have installed the burner.

Fig. 19 a-c Lamp mounts for gas discharge lamps

1 Upper clamping system, 2 Lower clamping system, 3 Cooling element 4 Melt nipple for the Hg 50 arc lamp, 5 Dust cover for the Xe 75 arc lamp

1

 

 

 

 

 

 

1

 

 

 

 

5

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

4

 

 

 

 

 

 

 

 

 

 

 

 

 

 

2

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Hg 50

 

a

Xe 75

 

 

 

 

 

b

 

 

 

 

3

 

3

 

 

 

 

 

2

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Hg 100 c

3

1

2

28

6. Assembly

Insert the lamp mount, with the burner installed, into the lamp housing and tighten it with the screws (20.8).

Close the lamp housing and retighten the fastening screws.

Place the lamp housing in the incident light lamp housing receptacle (21.1) and fasten it with the clamping screw on the side.

Connect the lamp housing to the external power supply (22.1).

Fig. 21 Rear view of the stand

1Incident light lamp housing receptacle

2Transmitted light lamp housing receptacle

312 V 100 W connection for transmitted light (symbol: )

412 V 100 W connection for incident light (symbol: )

Fig. 20 106/106 z lamp housing (on the side, open)

1Cover raised

2Collector

312 V 100 W lamp or

gas discharge lamp in mount

4Reflector (mirror)

5, 6, 7 Adjusting screw for x-y reflector

8Fastening screw for lamp mount

9Socket for contact plug

1

2

4

5

3

6

7

8

9

8

Fig. 22 Rear panel of the ebq 100 supply unit 1 Lamp connection

1

1

2

 

3 4

 

29

6. Assembly

6.7 Equipping the incident light turret disk

Fig. 23 Filter cube,

Fig. 24 Filter cube,

 

front side

back side

The positions in the turret disk are numbered. Depending on how they are equipped, the individual filter and/or reflector cubes are set in pre-assigned positions at the factory. For details, check the identification sheet included with your order.

Insert the filter and reflector cubes in the following manner:

Never fit the incident light turret disk while the microscope is in operation.

Remove the face plate from the upper part of the microscope (Fig. 25). Press the locking pin (25.2) to turn the turret disk. When the locking pin is released, the turret disk locks into place again.

With the holder facing you squarely, insert the filter cube or reflector cube into the holder as described in the identification sheet provided. To do so, place the filter or reflector cube on the right side and press it toward the left into the mounting (Fig. 26).

Press the locking pin (25.2) and turn the filter turret to the next click stop.

Make sure that the turret engages (the locking pin springs forward) and insert the next filter and/or reflector cube as described above.

When all filters and reflector cubes have been inserted, close the front cover plate again.

Fig. 25 Removing the front panel

1Filter receptacle

2Locking pin

3Front panel

Fig. 26 Inserting the filter or reflector cubes 1 Mounting

1

1

2

3

30

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