Gentek 3980323 User Manual

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User’s Manual

Purifier® HEPA Filtered Enclosures

& Purifier® Class I Filtered

Enclosures

Models

3980200, 3980201, 3980202, 3980203, 3980220, 3980221, 3980222, 3980223, 3980300, 3980301, 3980302, 3980303, 3980320, 3980321, 3980322, 3980323, 3980400, 3980401, 3980402, 3980403, 3980420, 3980421, 3980422, 3980423

Register your product online at www.labconco.com/productreg.html or return the attached card and receiveFREE LabbyWear!

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Labconco HEPA Filtered Enclosure Manual

Warranty

Labconco provides a warranty on all parts and factory workmanship. The warranty includes areas of defective material and workmanship, provided such defect results from normal and proper use of the equipment.

The warranty for all Labconco products will expire one year from date of installation or two years from date of shipment from Labconco, whichever is sooner, except the following:

Purifier® Delta® Series Biological Safety Cabinets and PuriCare® Animal Laboratory Research Enclosures carry a three-yearwarranty from date of installation or four years from date of shipment from Labconco, whichever is sooner.

Carts carry a lifetime warranty.

Glassware is not warranted from breakage when dropped or mishandled.

This limited warranty covers parts and labor, but not transportation and insurance charges. In the event of a warranty claim, contact Labconco Corporation or the dealer who sold you the product. If the cause is determined to be a manufacturing fault, the dealer or Labconco Corporation will repair or replace all defective parts to restore the unit to operation. Under no circumstances shall Labconco Corporation be liable for indirect, consequential, or special damages of any kind. This statement may be altered by a specific published amendment. No individual has authorization to alter the provisions of this warranty policy or its amendments. Lamps and filters are not covered by this warranty. Damage due to corrosion or accidental breakage is also not covered.

Limitation of Liability

The disposal and/or emission of substances used in connection with this equipment may be governed by various federal, state, or local regulations. All users of this equipment are required to become familiar with any regulations that apply in the user’s area concerning the dumping of waste materials in or upon water, land, or air and to comply with such regulations. Labconco Corporation is held harmless with respect to user’s compliance with such regulations.

.

Part #3905500, Rev. A

ECO C415

ABLE

F

ONTENTS

 

T

O C

 

CHAPTER 1: INTRODUCTION

1

About This Manual

 

2

Typographical Conventions

 

3

CHAPTER 2: PREREQUISITES

5

Support, Vibration & Movement Requirements

6

Location and Air Current Requirements

6

Exhaust & Blower Requirements

6

Electrical Requirements

 

7

Space Requirements

 

7

CHAPTER 3: GETTING STARTED

9

Unpacking Your Enclosure

 

10

Installing the Filtered Enclosure on a Supporting

 

Structure and Work Surface

10

Connecting to the Exhaust System (Optional)

13

Installation of HEPA Filters and Accessory Odor-

 

Control Carbon Filters

 

15

Connect the Electrical Supply Source to Filtered Enclosure

16

Set the Face Velocity with Speed Control Adjustment

17

Validating the Vented Enclosure

17

Sealing the Filtered Enclosure to the Work Surface

18

CHAPTER 4: HIGH PERFORMANCE FEATURES AND

 

SAFETY PRECAUTIONS

19

Components

 

 

20

Safety Precautions

 

27

CHAPTER 5: APPROPRIATE APPLICATIONS FOR YOUR

 

FILTERED ENCLOSURE

31

Routine Daily Work Procedures

31

Suitable Applications

 

33

HEPA Filter Applications, Suitability & Guidelines

33

Odor Control Carbon Filter Applications

34

Definition of Terms

 

35

Appropriate Chemicals for Odor Control Carbon Filters

36

Hazardous Misapplications for Odor Control Carbon

 

Filters with Volatile Chemicals

37

Chemical Carcinogen Use with Odor Control

 

Carbon Filters

 

37

Prohibited Acid Use

38

CHAPTER 6 MAINTAINING YOUR FILTERED ENCLOSURE 39

Routine Maintenance Schedule

40

Decontamination

41

Determination of when to Replace HEPA Filters

41

How to Install a New HEPA Filter

42

HEPA Filter Leak Test

44

Setting the Inflow Face Velocity with the Speed Control

 

Adjustment

46

Calibrate and Operate the Airflow Monitor

46

Determination of When to Replace Odor control

 

Carbon Filters and How to Replace

50

Calculating Odor control Carbon Filter Life

52

Initial Certification

53

Re-Certification

53

Fluorescent Light Replacement

54

UV Light Replacement

54

Motorized Impeller Replacement

54

Speed Control Replacement

56

CHAPTER 7 ACCESSORIZING & MODIFYING YOUR

 

FILTERED ENCLOSURE

57

CHAPTER 8 TROUBLESHOOTING & SERVICER

 

OPERATING LOG

63

APPENDIX A: FILTERED ENCLOSURE COMPONENTS

 

AND REPLACEMENT PARTS

67

APPENDIX B: DIMENSIONS AND EXHAUST OPTIONS

72

APPENDIX C: FILTERED ENCLOSURE SPECIFICATIONS

75

APPENDIX D: QUICK CHART FOR THE FILTERED

 

ENCLOSURES

81

APPENDIX E: REFERENCES ON VENTILAITON, SAFETY,

 

OCCUPATIONAL HAZARDS, BIOSAFETY

 

AND DECONTAMINATION

82

DECLARATION OF CONFORMITY

87

CHAPTER1

INTRODUCTION

Congratulations on your purchase of a Labconco Purifier® HEPA Filtered Enclosure or Purifier® Class I Filtered Enclosure. Your enclosure provides personnel protection through superior containment. It is the result of Labconco’s more than 50 years experience in manufacturing fume hoods and more than 30 years experience in manufacturing filtered enclosures.

These enclosures will effectively contain toxic, noxious, or biohazardous particulates when properly installed and operated. Each enclosure uses a single HEPA filter, which is rated at least 99.99% efficient for 0.3-micronparticles. Additionally, an accessory Odor Control carbon filter may be added to adsorb nuisance odors from organic vapors, formaldehyde or ammonia and amines. The Purifier enclosures offer many unique features to enhance safety, performance, and energy savings. To take full advantage of them, please acquaint yourself with this manual and keep it handy for future reference. If you are unfamiliar with how high performance HEPA filtered enclosures operate, please review

Chapter 4: High Performance Features and Safety Precautions before you begin working in the enclosure. Even if you are an experienced user, please review Chapter 5: Using Your HEPA Filtered Enclosure,which describes the Purifier features so that you can use the filtered enclosure efficiently. For hazardous biological applications requiring user protection but not product protection, Labconco recommends the purchase of a Class I enclosure, which includes a UV light for general surface decontamination. For general hazardous biological applications,

1

Chapter 1: Introduction

particulates, and nuisance powders, Labconco recommends the use of the Purifier HEPA Filtered Enclosure. See chart below and contact Labconco for additional ordering information.

No.

Application

Product

Filters

Bag-in/Bag-out

UV

HEPA

Light

 

 

 

 

1.

Nuisance Powders,

Purifier HEPA

HEPA

NO

 

 

Biohazardous

Filtered

 

Decontamination

NO

 

Materials

Enclosure

 

Required

 

2.

Nuisance Powders,

Purifier HEPA

HEPA

NO

 

 

Biohazardous &

Filtered

and

Decontamination

NO

 

Volatile Chemicals

Enclosure

Carbon*

Required

 

3.

Biohazardous

Purifier Class I

HEPA

NO

Included for

 

Materials & Nuisance

Enclosure

 

Decontamination

Surface

 

Powders

 

 

Required

Decontamination

4.

Biohazardous

Purifier Class I

HEPA

NO

Included for

 

Materials, Nuisance

Enclosure

and

Decontamination

 

Surface

 

Powders & Volatile

 

Carbon*

Required

 

 

Decontamination

 

Chemicals

 

 

 

 

 

 

 

 

* It is recommended that a Labconco product specialist review the chemical application to determine if it is suitable. Consult Chapter 5 and Chapter 6.

About This Manual

This manual is designed to help you learn how to install, use, and maintain your filtered enclosure. Instructions for installing optional equipment or accessory carbon filters on your filtered enclosure are also included.

Chapter 1: Introduction provides a brief overview of the filtered enclosure, explains the organization of the manual, and defines the typographical conventions used in the manual.

Chapter 2: Prerequisites explains what you need to do to prepare your site before you install the filtered enclosure. Electrical and service requirements are discussed.

Chapter 3: Getting Started contains the information you need to properly unpack, inspect, install, and certify the filtered enclosure.

Chapter 4: High Performance Features and Safety Precautions explains how the Purifier filtered enclosure operates and the appropriate precautions you should take when using it.

2

Chapter 1: Introduction

Chapter 5: Using Your Filtered Enclosure discusses the basic operation of how to prepare, use and shut down your filtered enclosure.

Chapter 6: Maintaining Your Filtered Enclosure explains how to perform routine maintenance on the filtered enclosure.

Chapter 7: Accessorizing Your Filtered Enclosure explains acceptable modifications to the filtered enclosure or how to add accessories.

Chapter 8: Troubleshooting contains a table of problems you may encounter while using the filtered enclosure including the probable causes of the problems and suggested corrective actions.

Appendix A: Components and Replacement Parts contains labeled diagrams of all of the components of the filtered enclosures.

Appendix B: Dimensions and Exhaust Options contains comprehensive diagrams showing all of the dimensions for the filtered enclosures.

Appendix C: Filtered Enclosure Specifications contains the electrical requirements for filtered enclosures. Wiring diagrams are also included.

Appendix D: Quick Chart provides a table of airflow and test data for the filtered enclosures.

Appendix E: References lists the various resources available that address laboratory ventilation and biosafety.

Typographical Conventions

Recognizing the following typographical conventions will help you understand and use this manual:

• Book, chapter, and section titles are shown in italic type (e.g.,

Chapter 3: Getting Started).

• Steps required to perform a task are presented in a numbered format.

Comments located in the margins provide suggestions,

 

reminders, and references.

 

Critical information is presented in boldface type in paragraphs

 

!

 

that are preceded by the exclamation icon. Failure to comply

 

 

 

 

 

 

 

 

 

 

 

 

3

Chapter 1: Introduction

with the information following an exclamation icon may result in injury to the user or permanent damage to the enclosure.

Critical information is presented in boldface type in paragraphs that are preceded by the wrench icon. A trained certifier or contractor should only perform these operations. Failure to comply with the information following a wrench icon may result in injury to the user or permanent damage to your hood.

• Important information is presented in capitalized type in paragraphs that are preceded by the pointer icon. It is imperative that the information contained in these paragraphs be thoroughly read and understood by the user.

 

 

 

 

 

 

 

 

• A number icon precedes information that is specific to a

 

2'

 

 

3'

 

 

4'

 

 

 

 

 

particular model of enclosure. The 2' icon indicates the text is

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

specific to the 2-footwide model. The 3' icon indicates the text

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

is specific to the 3-footmodel, etc.

4

CHAPTER2

PREREQUISITES

Before you install the filtered enclosure, you need to prepare your site for installation. You must be certain that the area is level and of solid construction. In addition, a dedicated source of electrical power should be located near the installation site to power the filtered enclosure, and other apparatus. Additionally, the enclosure should be strategically placed in the lab to provide efficient workflow.

Carefully read this chapter to learn the requirements for your installation site:

The support, vibration and movement requirements.

The location and air current requirements.

The exhaust and blower requirements.

The electrical power requirements.

The space requirements.

Refer to Appendix B: Purifier Dimensions for complete enclosure dimensions.

Refer to Appendix C: Purifier Specificationsfor complete filtered enclosure electrical and environmental conditions, specifications and requirements.

5

Chapter 2: Prerequisites

Support, Vibration and Movement Requirements

At a minimum, the supporting structure usually consists of a base cabinet and chemically-resistantwork surface.

A stand or mobile bench is not recommended if the enclosure will be used for precise weighing of powders.

Location and Air Current Requirements

The Purifier Filtered Enclosures have been designed to contain hazards by negating typical cross drafts and turbulence within the opening. However, as a precautionary safety measure and a higher level of quality management, it is recommended that the enclosure be placed in an area to avoid:

High traffic areas where walking might cause an air disturbance or be a nuisance.

Overhead or wall HVAC diffusers, fans, radiators or other lab equipment producing air currents.

Next to doorways or windows that may be opened.

Exhaust and Blower Requirements

The Purifier Class I Enclosure and Purifier Filtered Enclosure use an integral motorized impeller to draw room air past the operator and through the enclosure. This contaminated air is then pushed through the HEPA filter. The HEPA-filteredexhaust air is then forced out the top of the enclosure. An optional carbon filter may be installed on the downstream side of the HEPA filter to protect against nuisance odors.

The HEPA-filteredexhaust air can be recirculated into the laboratory or exhausted outside with the addition of the exhaust connection kit and remote blower listed in Chapter 7. Data for the exhaust volume and enclosure static pressure loss are listed for each filtered enclosure model at face velocities of 60, 75, 80, 90, 100, and 105 fpm.

6

Chapter 2: Prerequisites

 

 

 

 

 

Max.

 

 

 

 

 

 

Equivalent

Max.

 

 

Face

Exhaust

Noise

Resistance

External

Enclosure

 

Velocity

Volume

Pressure

of 6" Duct

Static

Width

Model Description

(fpm)

(CFM)

db(A)

(Ft)

Pressure

2'

2' Purifier Class I Enclosure

60

85

48-53

400

.20"

 

2' Purifier HEPA Filtered

75

110

49-55

220

.19"

 

Enclosure

80

115

50-56

200

.18"

 

 

90

130

51-57

140

.16"

 

 

100

145

53-58

80

.13"

 

 

105

155

54-59

70

.12"

3'

3' Purifier Class I Enclosure

60

130

48-53

130

.15"

 

3' Purifier HEPA Filtered

75

165

52-56

65

.12"

 

Enclosure

80

175

53-57

50

.11"

 

 

90

200

55-58

35

.09"

 

 

100

220

58-61

25

.08"

 

 

105

230

60-64

20

.07"

4'

4' Purifier Class I Enclosure

60

175

49-54

100

.20"

 

4' Purifier HEPA Filtered

75

220

54-58

60

.19"

 

Enclosure

80

235

58-61

50

.18"

 

 

90

265

60-63

35

.16"

 

 

100

295

62-66

25

.13"

 

 

105

310

64-67

20

.12"

Proper blower selection can be determined from these exhaust requirements and the total system static pressure loss. For outside exhaust, the enclosure must be connected to either a dedicated blower or a house exhaust system.

Labconco offers accessory remote blowers listed in Chapter 7.

Contact Labconco for blower sizing assistance.

If the enclosure is connected directly to a house exhaust system, an

adjustable damper (or valve) must be installed to control the ! airflow properly. This is equally important when a house exhaust

system is controlling multiple filtered enclosures. See Chapter 7 for accessory adjustable damper ordering information.

Electrical Requirements

Standard duplex electrical receptacles should be nearby for connecting the filtered enclosure, or other equipment. The enclosures include iris pass-throughsto allow electrical cords through the back of the enclosure without leaving a large hole for contaminants to escape.

Space Requirements

The dimensions for the different models are shown in Appendix B:

Dimensions

7

Chapter 2: Prerequisites

8

CHAPTER3

GETTINGSTARTED

Now that the site for your filtered enclosure is properly prepared, you are ready to unpack, inspect, install, and validate your system. Read this chapter to learn how to:

Unpack and move the enclosure.

Set up the enclosure with the proper supporting structure and work surface.

Connect to an exhaust system if applicable.

Installation of HEPA and accessory Odor Control carbon filters.

Connect the electrical supply.

Set the face velocity with the speed control adjustment.

Arrange validation for the enclosure.

Seal the enclosure to the work surface.

Depending upon which model you are installing, you may need common mechanical and electrical installation tools in addition to 5/16", 3/8", 7/16", and 1/2" wrenches, ratchets, sockets, a nut driver set, a flat-bladescrewdriver, a Phillips screwdriver, and a carpenter level to complete the instructions in the chapter.

Each enclosure model weighs between 125 to 195

!lbs. each (55 to 85 kg). The shipping container allows for lifting with a mechanical lift truck or floor jack. If you must lift the enclosure manually, follow safe-liftingguidelines.Do not

lift by the front air foil.

9

Chapter 3: Getting Started

The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen (15) days of delivery.

Unpacking the Enclosure

Carefully remove the shrink-wrapor carton on the enclosure and inspect it for damage that may have occurred in transit. If damaged, notify the delivery carrier immediately and retain the entire shipment intact for inspection by the carrier.

DO NOT RETURN GOODS WITHOUT THEPRIOR AUTHORIZATION OF LABCONCO.

UNAUTHORIZED RETURNS WILL NOT BE ACCEPTED.

IF ENCLOSURE WAS DAMAGED IN TRANSIT,YOU MUST FILE A CLAIM DIRECTLY WITH

THE FREIGHT CARRIER. LABCONCO CORPORATION AND ITS DEALERS ARE NOT RESPONSIBLE FOR SHIPPING DAMAGES.

Do not discard the packing material until you have checked all of the components and tested the enclosure.

We recommend that you do not remove the enclosure from its shipping container until it is ready to be placed into its final location. Move the unit by placing a flat, low dolly under the shipping skid, or by using a floor jack.

!Do not move the enclosure by tilting it onto a hand truck.

Installing the Filtered Enclosure on a Supporting Structure and Work Surface

Use caution when lifting or moving the enclosure.

When installing the enclosure onto a chemical-resistantwork surface or benchtop, ensure that the structure can safely support the combined weight of the enclosure and any related equipment. The work surface should be at least as wide as the enclosure to properly support it. The front of the enclosure should be aligned within

10

Chapter 3: Getting Started

0.36" of the front of the work surface. Mounting holes are provided in the Labconco accessory work surfaces to secure the enclosure.

Work Surface Specifications

The work surface should be smooth, rigid, and durable, such as a chemical-resistantepoxy resin. The surface should benon-porousand resistant to the biohazards, powders, solvents and chemicals used in conjunction with the Purifier Filtered Enclosure. The work surface should also contain a dished recessed area for containing primary spills.

Work Surface and HEPA Filtered Enclosure Installation

1.Level the base cabinets and the work surface. Work surface should be placed flush with the front of the base cabinet as shown in Figure 3-1.

2.Position the work surface in its intended location and with the front of the work surface towards you. (Rear mounting holes are located close to the rear edge.)

3.Secure the work surface to the base cabinet with a structural adhesive or silicone sealant.

4.Insert the supplied mounting screws in the four holes. Allow a minimum of 1/8" clearance under the head of the screw for positioning the enclosure.

5.Place the enclosure on the work surface and slide the rear flange and front air foil flanges under the mounting screw heads.

6.Tighten the four screws to complete the installation.

11

Chapter 3: Getting Started

Figure 3-1

Filtered Enclosure Installation

12

!

!

Chapter 3: Getting Started

Connecting to the Exhaust System (Optional)

WARNING: The weight of the exhaust ductwork system must be supported independently of the enclosure superstructure or damage may occur.

The exhaust system should be installed by a qualified HVAC contractor.

Exhaust transition kits aid in the removal of chemicals or applications where a higher degree of biohazard and particulate removal is required. See Chapter 7 for ordering exhaust transition kits for the Purifier HEPA Filtered Enclosure or Purifier Class I Enclosure. Review Chapter 2 for exhaust prerequisites and review Chapter 7 for ordering blower exhaust equipment. See Figure 3-2for exhaust kit options on Purifier filtered enclosures.

Consult Labconco Customer Service should you require help sizing your blower for the exhaust volume and system static pressure loss.

To ensure compatibility, the selected exhaust duct material should match the enclosure, procedures and chemical applications.

13

Chapter 3: Getting Started

Figure 3-2

Exhaust Duct Connection Kit shown installed on

Purifier Models (Optional)

Kit #3924400 (2'), 3924401 (3'), and 3924402 (4')

14

Chapter 3: Getting Started

Installation of HEPA Filters and

 

Accessory Odor Control Carbon

 

Filters

 

 

HEPA Filters

 

HEPA

 

 

HEPA filters are shipped installed with the gasket on the

 

downstream side. The HEPA filters are leak checked at Labconco.

 

A second leak check is recommended before using the enclosure

 

and at least annually thereafter. Consult your Safety Officer and

 

Chapter 6 for the HEPA Filter Leak Test. See Figure 4-2for

 

HEPA filter location, HEPA filter gasket, and filter clamp bolts.

 

The Purifier enclosures require decontamination of biohazardous

 

materials or are used with nonbiohazardous materials or nuisance

 

powders. All seams upstream of the HEPA filter are jacketed by

 

negative pressure. This intrinsically safe design ensures

 

containment.

 

Carbon Filters (Accessory for Odor Control only)

 

ACCESSORY

 

 

 

 

CARBON

 

 

 

 

 

 

 

 

 

For carbon filter installation, first remove the upper perforated cover, secured by Phillips head screws, on the top of the enclosure. See Figures 4-1and4-2for location of the upper perforated cover. See Chapter 7 for a list of available carbon filters. All carbon filters are simply gravity installed with the gasket side down and down stream on the exhaust side of the HEPA filter. Install the correct carbon filter for the application.

15

Chapter 3: Getting Started

HEPA

Carbon

Carbon

Carbon

Filter Type

Appropriate Use

 

HEPA

HEPA filters are high-efficiencyparticulate

2'

3707900

air filters having a particulate removable

3'

3707901

efficiency of 99.99% for particles with a

4'

3707902

diameter of 0.3 micron.

Organic Vapor

Adsorbs organic compounds designated by

Activated Carbon

NIOSH guidelines as acceptable for use with

2'

3937200

chemical cartridge-typerespirators.

3'

3937300

Concentrations in the enclosure’s work area

4'

3937400

must not exceed the IDLH for the chemical

 

 

and the exhaust from the enclosure must not

 

 

exceed the TWA.

Formaldehyde

Formaldehyde requires the use of an

(Formalin)

impregnated carbon for the treatment of

2'

3937201

formaldehyde. Concentrations in the

3'

3937301

enclosure’s work area must not exceed the

4'

3937401

IDLH for the chemical and the exhaust from

 

 

the enclosure must not exceed the TWA.

Ammonia and

Treats ammonia, low molecular weight

 

Amines

amines and other bases designated by NIOSH

2'

3937202

as acceptable for use with ammonia cartridge

3'

3937302

type respirators. Requires the use of an

4'

3937402

impregnated carbon. Concentrations in the

 

 

enclosure’s work area must not exceed the

 

 

IDLH for the chemical and the exhaust from

 

 

the enclosure must not exceed the TWA.

Important: Carbon filters do not provide any particulate protection, but provide odor control.

Connecting the Electrical Supply Source to the Filtered Enclosure

115V Models

Simply connect the 115V power cord supplied to the IEC electrical supply plug on the back of the enclosure. If using at 50 Hz operations, blower performance maximum airflow will be reduced by 17%.

230V Models

The above procedure applies for the 230V except it is shipped without a plug. Install the appropriate plug for your electrical specifications per local codes.

16

Chapter 3: Getting Started

Set the Face Velocity with the Speed Control Adjustment

Adjustment of the speed control gives the correct face velocity and is located behind the front panel. The face velocity should be from 75 to 105 fpm for biohazardous operations. (Consult your Safety Officer for airflow recommendations for your application). Containment is maximized at a setting within this range. Working at the lowest face velocity appropriate for the application will give the quietest operation. Face velocity measurements are made using an anemometer. An electric anemometer can be obtained from your laboratory supply dealer. Face velocity measurements should be taken in accordance with the Industrial Ventilation Manual. Using a small Phillips screwdriver, adjust the speed control to give the required face velocity. See Figures6-1and6-2in Chapter 6 to locate the speed control. The face velocity is increased by turning the speed control counter clockwise and clockwise to decrease face velocity.

Validating the Vented Enclosure

To determine the actual face velocity at the sash opening, airflow velocity readings are taken. This should be done across the sash opening of the enclosure in accordance with the Industrial Ventilation Manual. (See Appendix E) The “average face velocity” is achieved by taking readings in two rows across the enclosure with the readings 6" from the ends and evenly spaced every 12"; the first row is 3" down from the upper sash foil and the second row is 3" up from the work surface. A total of four readings will be taken for the 2' enclosure, six readings taken for the 3' enclosure and eight readings taken for the 4' enclosure and then averaged. Refer to Chapter 2 for proper airflow volumes for your particular model.

The Purifier enclosures have been tested at Labconco’s airflow test facility per ASHRAE 110-1995.All enclosures achieve an “as manufactured rating” of less than 0.05 part per million (ppm) at 4 liters per minute (lpm); AM <0.05 (Consult Labconco for individual ratings). For “field use” ASHRAE testing contact Labconco for a certifiedon-sitecontractor. Labconco also performed extensive ASHRAE testing to validate the enclosures for gas containment. For copies of these validation reports, contact Labconco Customer Service.

17

Chapter 3: Getting Started

The Purifier Enclosures were also subjected to Biological Containment testing, using a modified Personnel Protection Test, as described in NSF/ANSI Standard Number 49. An aerosol challenge of approximately 5 x 108 spores ofBacillus subtilus var Niger were released in the enclosure and appropriate air sampling equipment established the number of spores that escaped through the sash opening. Each size enclosure was tested three times. All three sizes of the enclosure were tested at 75 FPM inflow and typically 1 or less than one spore escaped from the enclosure during each of the tests.

The Purifier Enclosures were also subjected to an industrial hygiene air-monitoringstudy to confirm the performance of the enclosure for powders. The Purifier Enclosures demonstrated excellent containment when used by an operator using excellent technique and good containment when used by an operator using marginal technique. While no enclosure can compensate for improper technique, these tests confirm the Purifier Enclosures provide a safe working environment.

NOTE: Face velocity profiles and smoke testing ! should be performed frequently per your

organization’s quality system to ensure safe performance.

Sealing the Filtered Enclosure to the Work Surface

When the filtered enclosure has been set in place, ducted, (if necessary), and wired, it may be sealed at the work surface to prevent spilled materials from collecting under the walls. A bead of silicone sealant is recommended to seal the filtered enclosure to the work surface.

18

CHAPTER4

HIGHPERFORMANCE

FEATURESANDSAFETY

PRECAUTIONS

High Performance Features:

The patented* Purifier HEPA Filtered Enclosure and Purifier Class I Enclosure are designed to meet the needs of the laboratory scientist, and provide superior containment while operating at velocities between 75-105feet per minute. The filtered enclosures have been tested to effectively contain toxic, noxious, and biohazardous materials when properly installed and operated. What makes the filtered enclosures so unique is the revolutionary way they direct air into and through the contaminated air chamber. Labconco engineered the filtered enclosures to minimize the effects of turbulence. Thecontainment-enhancingand aerodynamic designs of the upper sash foil, side air foils, lower air foil, upper dilution air supply, and rear perforated baffle all work in concert to produce horizontal airflow patterns that significantly reduce powder, chemical and biological material concentrations through the work area.

*(U.S. Patent No. 6,461,233)

19

Chapter 4: High Performance Features and Safety

These concentrations of materials are predominantly removed on the “first pass” of airflow through the chamber resulting in high performance containment.

The plenum and the HEPA filter are jacketed by negative pressure. Should a leak occur in the filter gasket or the plenum, the contaminated air is recaptured and refiltered.

The Purifier Class I Enclosure and Purifier HEPA Filtered Enclosure have an access port located behind the front panel. For HEPA filter removal in biohazardous applications on Purifier enclosures, the HEPA filter will require decontamination before removal. Users of all equipment are encouraged to routinely check airflow or purchase a model with an airflow monitor.

20

Chapter 4: High Performance Features and Safety

17

26

16

19

15

10

3

8

18

2

4

6

1

5

Not Shown 7, 9, 10, 11, 12, 13, 20, 21, 22, 23, 24, 25, 28 (See

Figure 4-2)

Figure 4-1

21

Chapter 4: High Performance Features and Safety

Figure 4-2

HEPA Filtered Enclosure Airflow Diagram

22

Chapter 4: High Performance Features and Safety

1.Aerodynamic Clean-Sweep™Air Foilhas a unique shape that allows air to sweep the work surface for maximum containment. The Clean-Sweep™ openings create a constant protective barrier from contaminants. Should the operator inadvertently block the airflow entering the air foil, air continues to pass under the air foil and through the Clean-Sweep openings. See Figures 4-1 and 4-3.

Figure 4-3

2.Containment-EnhancingUpper Sash Foilincludes an open air passage directly atop the sash foil to bleed air into the hood chamber and direct chemical, powder and particulate materials away from the sash opening. The radiused sash foil sweeps airflow into the hood with minimal turbulence. See Figures 4-1 and 4-4.

Figure 4-4

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Chapter 4: High Performance Features and Safety

3.Upper Dilution Air Supply provides bypass air from above the work area. This feature constantly bathes the inside of the sash with clean air and reduces powders, particulate materials and chemical fumes along the sash plane, near the critical breathing zone. Five to seven percent of the required air volume is introduced through the upper dilution air supply. The upper dilution air supply also reduces stagnant pockets of air in the upper interior. See Figure4-2.

4.Zoned Rear Perforated Baffle directs horizontal laminar air streams to thethree-zonedsections of the perforated baffle. Thethree-zonedsections have increasingly more open area at the bottom that help form laminar airflow. This minimizes the potential for air to roll forward preventing contaminants from moving toward the sash opening. The majority of contaminants are highly diluted, captured and removed on the first pass through the enclosure. See Figures4-1and4-2.

5.Side-Entry Air Foils allowturbulence-freeair to enter the enclosure from the sides and allow clean air to sweep the interior walls of the enclosure. See Figures4-1and4-2.

6.Ergonomic Slope of 10 degrees provides maximum visibility, and comfort reduces glare, thereby minimizing operator fatigue. See Figure4-1.

7.Internal Depth of 23" provides necessary depth to support modern balances and other auxiliary equipment without extending outside the enclosure or resting on the lower air foil. See Figure4-1

8.Safety Glass Sash with Spring-LoadedLatchhas a wiping seal to contain contaminants and features a springloaded latch to secure sash open for loading and cleaning. The sash must be down for normal operation. See Figure 4-1.

9.Utility Ports with Iris allows electrical cords and data cords to pass through the back of enclosure without leaving a large hole for contaminants to escape. The enclosure ships with solid plugs. Iris plugs are included with the User’s Manual. See Figure4-1and4-2.

10.Accessory Guardian™ Airflow Monitor continuously monitors airflow. An audio/visual alarm alerts the user to low airflow conditions. The Guardian™ Airflow

24

Chapter 4: High Performance Features and Safety

Monitor is an available option on all Purifier models. See

Figure 4-1and4-2.

11.Inherently Safe Impeller has a negative pressure plenum that surrounds the positive pressure impeller so that if a leak should occur, the unfiltered air is captured and refiltered. See Figure4-2.

12.Vibration-IsolatedMotorized Impellerhas vibration isolation supports, which eliminates transfer of vibration to the work surface. This is extremely useful in weighing operations with precision balances. The impeller wheel is also dynamically balanced. See Figure 4-2.

13.Speed Control regulates the speed of the motorized impeller and is used by the certifier to validate and adjust the inflow velocity. Located behind the front panel. See Figure4-2.

14.Space-Saving Design increases effective laboratory workspace, because the impeller and HEPA filter are contained within the enclosure, a separate filtered blower module is not required. See Figure4-1

15.Fluorescent Lamp is located above the work area, out of contact with contaminated air. A safety glass window beneath the lamp distributes the light evenly across the work surface. See Figure4-1.

16.Filter System Pressure Gauge indicates the total system pressure across the HEPA filter and pressurized plenum to help predict HEPA filter loading. See Figure 4-1.

17.Filter Clamping Bolt evenly seals the HEPA filter to the frame of the enclosure. See Figure4-1and4-2.

18.Sash. The sash is constructed of 1/4 inch tempered safety glass. It pivots up for loading and cleaning. See Figure4-1

19.Control Panel. The control panel, which is located above the sash, contains the filter system pressure gauge, control switches and the electronics. See Figure4-1.

20.HEPA (High Efficiency Particulate Air) Filter is rated to remove 99.99% of all particles 0.3 micron in size. See Figure 4-2.

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Chapter 4: High Performance Features and Safety

21.Lamp Ballast (Not Shown) for the fluorescent lamp is located behind the control panel.

22.UV (Ultraviolet) Lamp (Not Shown), found only on Purifier Class I Enclosure, allows the operator to surface disinfect the work area of the enclosure when it is not in use. The UV will not penetrate the plane of the sash and lower airfoil.

23.Accessory Work Surface (Not Shown) is dished and contoured to fit the dimensions of the enclosures to contain spills.

24.Accessory Odor-ControlCarbon Filtersare available for nuisance odor control. Accessory carbon filters are designed to remove trace amounts of noxious fumes; capacity is not adequate to remove continuous fume generation. The carbon filters are supported above the HEPA filter and the gasket side is down and compressed by the weight of the carbon filter. Carbon filters are listed in Chapter 7. See Figure 4-2.

25.Accessory Exhaust Connection Kits are available to duct the Purifier Class I Enclosure and Purifier HEPA Filtered Enclosure to the outside. The exhaust connection allows the operator additional protection from hazardous fumes or vapors and should be used if the carbon filter capacity is not adequate. Each kit includes an epoxy-coated steel exhaust transition adapter with manual adjustable damper designed for 6" diameter PVC duct. See Figure 3-3 in Chapter 3.

26.Upper Diffuser Screen provides protection for the HEPA Filter and (LED) airflow sensor. See Figure4-1and4-2.

27.HEPA Filter Access Port to access HEPA filter during filter changing procedures. For harmful powders and particulates, the truebag-in/bag-outprocedure is used to properly dispose of the contaminated HEPA filter. See Figure4-2.

28.Utility Shelves (Not Shown) allow the user to hold utensils and printers on shelves inside the enclosure. See Chapter 7.

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Chapter 4: High Performance Features and Safety

Safety Precautions

1.Although the enclosure has been engineered to maintain optimum operator safety, caution should always be used while working. Prior to using the enclosure, check to make sure that the exhaust blower is operating and that air is entering the enclosure at its specified face velocity. The use of an airflow monitor is recommended to alert the user to a problem with airflow.

2.Use good housekeeping in the enclosure at all times. Clean up spills immediately. Periodically clean enclosure interior.

3.Do not overload the work surface with apparatus or work material. The safe operation of the enclosure is based upon having proper airflow through the structure. Do not place large objects directly on the work surface. Instead, elevate the object 3/4" on blocks to allow a flow of air under the object and into the rear baffle exhaust slots. Ensure blocks are level and secured in place.

4.Blocking large portions of the rear baffle will change the airflow pattern in the enclosure causing turbulence. (Do not store containers or supplies against the rear baffle, as this will affect airflow).

5.Always work with your hands as far back into the enclosure as possible. Keep all biohazardous materials, chemicals and apparatus inside the lower air foil of the enclosure.

6.Do not work in this enclosure without the exhaust system running.

7.Perchloric acid use in this enclosure is prohibited.

8.High-levelradioisotope materials are prohibited in this enclosure. Consult your Safety Officer.

9.Avoid cross drafts and limit traffic in front of the enclosure. Air disturbances created may draw contaminants out of the enclosure.

10.A qualified certification technician should test the enclosure before it is initially used. The enclosure should be validated whenever it is relocated.

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Chapter 4: High Performance Features and Safety

11.The use of safety goggles, protective clothing, gloves and any other personal protective equipment recommended by your safety officer should be used.

12.The sash must remain in the down position while using the enclosure.

13.Proper performance of the enclosure depends largely upon its location and the operator’s work habits. Consult the references in Appendix D.

14.The enclosure should be recertified whenever it is serviced or at least annually thereafter.

15.The HEPA filter provides personnel and environmental protection from particulate matter. Because room air is drawn over the work surface during operation, this enclosure should not be used for operations requiring product protection from environmental contamination.

16.Avoid the use of flammable gases or solvents in the enclosure if possible. Care must be taken to ensure against the concentration of flammable or explosive gases or vapors. Use of an open flame should be avoided in the enclosure. Open flames may disrupt the airflow patterns in the cabinet, burn the HEPA filter and damage the filter’s adhesive. Gases under high pressure should not be used in the enclosure as they may disrupt the airflow patterns of the cabinet.

17.HEPA filters are only effective for entrapment of particulate matter. Manipulations that generate gases or vapors from toxic chemicals or radionuclides, must be evaluated carefully from the standpoint of buildup to dangerous levels, the decontamination of the enclosure and compliance with applicable regulations.

18.The surface of the HEPA filter is fragile and should not be touched. Care must be taken to avoid puncturing the HEPA filter during installation or normal operation. If you suspect that a HEPA filter has been damaged DO NOT use the enclosure; contact a local certification agency or Labconco.

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Chapter 4: High Performance Features and Safety

19.The HEPA filter in the enclosure will gradually accumulate airborne particulate matter from the room and from work performed in the enclosure. The rate of accumulation will depend upon the cleanliness of the room air, the amount of time the enclosure is operating and the nature of work being done in the enclosure. With normal usage, the HEPA filters will last two to five years before requiring replacement.

20.Avoid direct exposure to ultraviolet (UV) radiation; NEVER work in the Purifier Class I Enclosure when the UV light is on.

21.Ensure that the filtered enclosure is connected to an electrical service in accordance with local and national electrical codes. Failure to do so may create a fire or electrical hazard. Do not remove or service any electrical components without first disconnecting the filtered enclosure from electrical service.

22.Use of an open flame must be avoided with the enclosure. Open flames may disrupt the airflow patterns in the enclosure and cause a fire hazard with volatile solvents.

23.Increase the face velocity by adjusting the speed control of the motorized impeller or change the HEPA filter when the face velocity falls below acceptable limits established by your Safety Officer.

24.Exhaust carbon filters and HEPA filters are disposed as hazardous waste. The user is responsible for recording the chemicals adsorbed or treated by the filters and disposing properly. HEPA filters should be decontaminated before disposal on Purifier enclosures.

25.Ensure only trained operators use the enclosure. New users should review the User’s Manual and become familiar with the operation of the enclosure.

26.If the filtered enclosure is to be used in a confined space, make sure the space is well ventilated and the concentration of toxic contaminants cannot accumulate greater than the TWA.

27.Proper operation of the enclosure depends largely upon the enclosure location and the operator’s work habits. The enclosure should be located away from traffic patterns, doors, fans, ventilation registers, fume hoods, and any other air-handlingdevice that could disrupt its airflow patterns. ConsultChapter 2: Prerequisites andChapter 3: Getting Started sections of this manual for further details.

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Chapter 4: High Performance Features and Safety

28.Only nuisance powders, biohazardous and particulates removed by HEPA filters and trace chemicals which can be safely adsorbed and treated with specific carbon based filters are appropriate for use in this enclosure.

29.The warning properties (i.e., odor, taste) of the volatile organic compounds or other material being used in the enclosure must be adequate to provide an early indication that the carbon filter may be saturated or inadequate. Contact Labconco for help with chemical assessment.

30.Use the smallest possible quantity of chemical(s) within the enclosure and never exceed the amount, which can be effectively adsorbed by the Odor Control carbon filter before breakthrough.

31.Adjust face velocity to fall between 75 and 105 FPM for biological procedures.

32.Leave the blower on for at least one minute after work in the enclosure has been completed.

33.If a chemical, powder or particulates are spilled on the work surface, DO NOT switch off the blower until all traces have been removed.

34.Tag enclosures with appropriate warning, if filters have been removed for service.

35.If the blower fails during use, processes should cease and the area should be vacated and ventilated.

36.Always refer to the NIOSH Pocket Guide to Chemical Hazards before proceeding.

30

CHAPTER5

APPROPRIATE

APPLICATIONS FOR YOUR

FILTEREDENCLOSURE

Now that the installation of your filtered enclosure is completed, you are ready to use your filtered enclosure. Read this chapter to learn about:

1.Routine Daily Work Procedures.

2.Suitable Applications.

3.Appropriate HEPA Filter Applications, Suitability and Guidelines.

4.Odor Control Carbon Filter Applications.

5.Definition of Terms.

6.Appropriate Chemicals for Odor Control Carbon Filters.

7.Hazardous Misapplications for Odor Control Carbon Filters.

8.Chemical Carcinogen use with Odor Control Carbon Filters.

9.Prohibited Acid Use.

Routine Daily Work Procedures

Planning

Thoroughly understand procedures and equipment required before beginning work.

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Chapter 5: Using Your Filtered Enclosure and Appropriate Application

Arrange for minimal disruptions, such as room traffic or entry into the room while the enclosure is in use.

Start-up

Turn on exhaust system and accessory light. Turn off the UV light, if equipped.

Only raise the sash for loading and cleaning.

Check the baffle air slots for obstructions.

Allow the enclosure to operate unobstructed for 1 minute.

Wear a long sleeved lab coat and rubber gloves. Use protective eyewear. Wear a protective mask if appropriate. Consult your Safety Officer for additional personal protective equipment recommendations.

Loading Materials and Equipment

Load only the materials required for the procedure. Do not overload the enclosure.

Do not obstruct the air foil, or rear baffle slots.

Large objects should not be placed close together and should be elevated above the work surface to permit airflow to sweep under the equipment.

After loading, wait one minute to purge airborne contaminants from the work area.

Work Techniques

Keep all materials inside the lower air foil, and perform all contaminated operations as far to the rear of the work area as possible.

Segregate all clean and contaminated materials in the work area.

Avoid using techniques or procedures that disrupt the airflow patterns of the enclosure.

Final Purging

Upon completion of work, the enclosure should be allowed to operate for two to three minutes undisturbed, to purge airborne contaminants from the work area before shutting down the blower.

If equipped, turn on the UV light to decontaminate the work area as determined by your Safety Officer.

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Chapter 5: Using Your Filtered Enclosure and Appropriate Application

Unloading Materials and Equipment

Objects in contact with contaminated material should be surface decontaminated before removal from the enclosure.

All open trays, weigh vessels or containers should be covered before being removed from the enclosure.

Shutdown

Turn off the exhaust system and light.

Turn off the UV light, if applicable.

Suitable Applications

NIOSH (National Institute for Occupational Safety and Health, USA) has established guidelines for chemical cartridge and HEPA filtered respirators. Suitable applications for the filtered enclosures are based on these guidelines. As with respirators, chemical contaminants are adsorbed or treated by carbon; particulates, powders and biohazardous materials are filtered by the HEPA filter before air is returned to the room. Biohazardous materials or other hazardous solids can be used with the HEPA filtered enclosure as long as appropriate decontamination occurs before filter changing operations. See Chapter 6 for decontamination procedures.

Listed below are suitable applications for HEPA filters and accessory Odor Control Carbon Filters.

HEPA Filter Applications,

Suitability and Guidelines

Biohazardous materials and hazardous solids to protect only the operator and the surrounding environment.

Weighing of powders.

Release of particulates using a HEPA filter.

Powder or particulate procedures traditionally performed on an open bench.

The HEPA filtered enclosure provides personal and environmental protection from particulate matter, hazardous powders and biohazardous materials. Because room air is drawn through the enclosure during operation, this enclosure should not be used for operations requiring sample protection from environmental contamination.

HEPA filters are only effective for entrapment of particulate matter, hazardous powders and biohazardous materials. Manipulations that generate gases or vapors, i.e., toxic chemicals or radionuclides, require the use of a HEPA and carbon filter in combination. Otherwise the enclosure should

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Chapter 5: Using Your Filtered Enclosure and Appropriate Application

be ducted to the outside. The surface of the HEPA filter is fragile and should not be touched. Care must be taken to avoid puncturing the HEPA filter during installation. If you suspect that a HEPA filter has been damaged, DO NOT use the enclosure. See Chapter 6 for Replacing the HEPA Filter.

The HEPA filter will gradually accumulate airborne particulate matter from the room and from work performed in the enclosure. The rate of accumulation will depend upon the cleanliness of the room air, the amount of time the enclosure is operating, and the nature of work being done. With normal usage, the HEPA filters will last two to five years before requiring replacement.

Each filtered enclosure is provided with a HEPA filter. For biohazardous materials, follow the Decontamination Procedure in Chapter 6 for disposal instructions.

Odor Control Carbon Filter Applications (sold as an accessory)

Release of low concentrations of vapors effectively adsorbed or treated in carbon based filters.

Treatment of low-levelcarcinogens or suspected carcinogens. (See Chemical Carcinogens in Chapter 5)

Procedures that may have traditionally been done on the open bench (low levels only).

Odoriferous chemicals that are an unpleasant nuisance.

Other applications, not fitting the above guidelines, would be better suited with the exhaust ducted to the outside. The exhaust connection kit (See Chapter 3 and Chapter 7) can be added to the filtered enclosures for ducting to the outside. Labconco also manufactures fume hoods and Class II Biological Safety Cabinets for exhausting to the outside. Consult Labconco product specialist for additional information.

REFERENCES TO NIOSH OR OSHA

GUIDELINES AND REGULATIONS APPLY TO

ANY WORK PLACE UNDER THE JURISDICTION OF THE U.S. DEPARTMENT OF LABOR. OTHER COUNTRIES OUTSIDE THE U.S. HAVE ESTABLISHED STANDARDS, WHICH MAY DIFFER SLIGHTLY FROM

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Chapter 5: Using Your Filtered Enclosure and Appropriate Application

THOSE USED AS GUIDELINES FOR THIS

PRODUCT. IT IS THE USER’S

RESPONSIBILITY TO BECOME AWARE OF

LOCAL REGULATIONS GOVERNING THE

SAFE USE AND DISPOSAL OF CHEMICALS,

CARBON AND HEPA FILTERS. KNOWLEDGE

OF ESTABLISHED SAFE EXPOSURE LEVELS

IS IMPERATIVE TO THE PROPER USE OF

FILTERED ENCLOSURES.

Definition of Terms

NIOSH – National Institute for Occupational Safety and

Health/Mine Safety and Health Administration. (U.S.A.)

TWA – Recommended Exposure Limits expressed as a Time Weighted Average. The exposure limit for that chemical for up to a10-hourworkday, 40 hours a week. Expressed in units of parts per million or milligrams per cubic meter.

Odor Threshold – The value in parts per million or milligrams per cubic meter for which one might expect to smell a chemical’s presence in the air. This value is very subjective and detection will vary with the sensitivity of one’s nose. The period of time until the odor threshold is reached in the exhaust stream can be estimated from Labconco exclusive computerized filter modeling program. Contact Labconco on carbon filter life for specific applications. See Chapter 6.

Saturation Level or Time – There is a limit to the amount of chemical that can be adsorbed by activated carbon, or neutralized bychemically-treatedcarbon. Once the capacity of the carbon is reached, it is considered to be saturated and will adsorb (or neutralize) no further material; the outlet concentration of the chemical will equal the inlet concentration from that point until the filter is replaced. (Note that the capacity of activated carbon is not a constant, but varies with the inlet concentration). Labconco Technical specialists can determine with the computerized carbonmodeling program the estimated time saturation for a particular chemical. When using a HEPA filter alone in all filtered enclosures or in combination with an Odor Control carbon filter, the speed control will need to be increased to allow for HEPA filter loading. Measure filtered enclosure face velocity with an anemometer.

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Chapter 5: Using Your Filtered Enclosure and Appropriate Application

IDLH (Immediately Dangerous to Life and Health). An atmosphere that poses an immediate hazard to life or produces immediate irreversible health effects. IDLH concentrations should not be approached in the enclosure.

Appropriate Chemicals for Odor Control Carbon Filters

Below is a general set of rules to determine appropriateness of chemical usage.

Selected organic chemicals considered to be ! occupational carcinogens by NIOSH can be used

in the filtered enclosure with carbon filters under rigid restrictions. See separate discussion on carcinogens for special instructions.

Organics must have time weighted exposure limits (TWA) of 1

PPM or greater.

Chemicals must have a detectable odor at concentrations below the

TWA for the chemical.

Chemicals must be designated by NIOSH guidelines as acceptable for use with chemical cartridge-typerespirators (the exception is formaldehyde and ammonia/amines, which used impregnated carbon). Chemicals not listed by NIOSH in the Pocket Guide must be approved by Labconco Product Specialist (or Engineering).

Inlet concentration must never exceed the IDLH (Immediately

Dangerous to Life and Health) concentrations.

Chemicals having a recommendation by NIOSH of at least “Escape GMFOV” (Gas Mask Full-FaceRespirator).

When evaporating a mixture of chemicals, the chemical having the lowest TWA will be used to determine if the mixture meets the guidelines.

Call a Labconco Product Specialist at 1-800-821-5525for assistance in chemical appropriateness.

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Chapter 5: Using Your Filtered Enclosure and Appropriate Application

Hazardous Misapplications for Odor Control Carbon Filters with Volatile Chemicals

There is one scenario where the accessory carbon filter misapplication would be a part of a hazardous condition. If the user continues to operate the enclosure with any of the following conditions present a potentially hazardous condition will exist:

1.The inlet concentration of vapors is greater than the TWA.

2.The carbon filter becomes saturated.

3.The ventilation of the room is insufficient to dilute the exhaust of the enclosure to below the TWA for the

chemical.

When the inlet concentration is greater than the TWA, extra measures must be taken to monitor the filter and number of room air exchanges.

Chemical Carcinogen Use with Odor Control Carbon Filters

Selected carcinogens may be used safely with Odor Control carbon filters under the following restrictions.

The use of a vented fume hood or ventilated

!enclosure with ducting to the outside is always the preferred method when working with carcinogens. The Odor Control carbon filters should only be used, as a last resort when venting to the outside is not an option.

The potential carcinogens are listed in the NIOSH Pocket Guide to Chemical Hazards as “Ca.” Each potential carcinogen must have a TWA of 1 or greater; have minimum respirator recommendation of Escape GMFOV, and an odor threshold significantly lower than the TWA for the chemical.

The inlet concentration or the evaporation rate of the chemical must never exceed the TWA for the chemical.

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Chapter 5: Using Your Filtered Enclosure and Appropriate Application

Consult a Labconco Technical Specialist for estimated saturation life. See Chapter 6 for an example of estimating saturation life. Another source is the Labconco chemical guide for carbon filtered enclosures.

Prohibited Acid Use

The Purifier HEPA and Purifier Class I filtered enclosures motorized impeller cannot be exposed to acids. Where applications require the use of acids, a separate ventilated enclosure or vented fume hood should be used with a remote blower ducted to the outside.

No exceptions are permitted, as the impeller life span will be limited with acid use.

38

CHAPTER6

MAINTAININGYOUR

FILTEREDENCLOSURE

Monitoring airflow and changing the filters is the primary maintenance required. Decontamination may be required and is reviewed in Chapter 6. Certification and recertification is reviewed in Chapter 6.

Review this chapter on maintenance for the following:

1.Routine Maintenance.

2.Decontamination.

3.Determination of when to replace the HEPA filters.

4.How to install a new HEPA filter.

5.HEPA filter leak test.

6.Speed control adjustment and setting the inflow face velocity.

7.Operating and calibrating the airflow monitors.

8.Determination of when to replace Odor Control carbon filters and how to replace.

9.Calculating Odor Control carbon filter life.

10.Initial certification.

11.Re-certification.

12.Fluorescent light replacement.

13.UV light replacement on Purifier Class I.

14.Motorized impeller replacement.

15.Speed control replacement.

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Chapter 6: Maintaining Your Filtered Enclosure

HEPA

CARBON

CARBON

Routine Maintenance Schedule

Weekly

Wipe down the interior surfaces of the enclosure with a disinfectant or cleaner, depending upon the usage of the unit and allow to dry.

Using a damp cloth, clean the exterior surfaces of the enclosure, particularly the front and top to remove any accumulated dust.

Operate the exhaust system, noting the airflow velocity through the enclosure using a source of visible smoke. Airflow monitors are recommended for constant monitoring.

Monthly (or more often as required)

Determine the actual face velocity through the sash opening of the enclosure where the average reading should be at the specified velocity. (Use calibrated thermal anemometer or other approved apparatus). Airflow alarms are recommended for constant monitoring.

The enclosure rear baffle should be checked for any blockage to ensure that the enclosure is maintaining proper airflow.

All weekly activities.

Check face velocity. Increase speed control or change HEPA filter when face velocity of the enclosure drops below the recommended speed for your facility or if the airflow alarm monitor alerts you. Airflow monitors are recommended.

While the enclosure is filled with the contaminant, test filter condition on Odor Control carbon filters using the appropriate gas detector tube at intervals of 20% of the total estimated time. The exception to the 20% recommendation is formaldehyde or any carcinogen or suspected carcinogen. These hazardous chemicals must be checked at least every 10% of the total estimated time. Gas detector tubes for the specific chemicals that are being used in the enclosure can be obtained from your laboratory supply dealer.

Replace Odor Control carbon filters when chemical breakthrough is indicated by odor, time, detector tube, or for some chemicals, analytical instrumentation. See “Replacing Odor Control Carbon Filters” section of this manual in Chapter 6.

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Chapter 6: Maintaining Your Filtered Enclosure

Annually

Replace the fluorescent lamps. Replace UV lamps if equipped.

Have the enclosure validated by a qualified certification technician. See Certification and Recertification in Chapter 6.

All monthly activities.

Decontamination

When used in conjunction with biohazards, the Purifier Class I Enclosure and Purifier HEPA Filtered Enclosure should be decontaminated with formaldehyde gas before:

maintenance work in contaminated areas

HEPA filter changes

moving the cabinet to a new location

changing research programs

after a gross spill of biohazardous material

The procedures for performing a gaseous decontamination are thoroughly outlined in the U.S. Department of Health, Education and Welfare booklet entitled Formaldehyde Decontamination of Laminar Flow Biological Safety Cabinets, available from NIH, Division of Safety, Bethesda, MD 20892, call301-496-2801,or from Labconco Corporation.

Determination of when to Replace

HEPA Filters

The HEPA filters in the filtered enclosures gradually accumulate airborne particulate matter and powders from the enclosure and room. The rate of accumulation will depend upon the cleanliness of the room air, the amount of time the enclosure is operating, and the nature of work being done in the enclosure. In typical installations and usage, the HEPA filters will last two to five years before requiring replacement. Replace HEPA filters when face velocity drops below the recommended 75-105fpm velocity, and the speed control is adjusted to full speed. Replace HEPA filters if it fails the HEPA Filter Leak Test in Chapter 6.

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Chapter 6: Maintaining Your Filtered Enclosure

How to Install a New HEPA Filter

NOTE: The enclosure must be properly decontaminated before servicing the HEPA filter. Only a qualified certifier should service the HEPA filter. After the HEPA filter is replaced, the enclosure MUST be certified. See Figure 6- 1.

1.Unplug the enclosure.

2.Remove the front panel by loosening the two screws that secure it, and then remove the filter access cover.

3.Using a 9/16" deep socket, loosen the filter clamp bolts located on top. Refer to Figures 4-1and4-2.

4.With the clamp bolts loosened, the HEPA filter-clampingframe should be clear of filter. Carefully pull the filter straight out of the enclosure and discard properly.

5.With the filter removed, inspect the clamping frame and the frame of the cabinet for damage.

6.Cover the surface of the new HEPA filter gasket with a light coating of silicone grease, if desired.

7.Install the new HEPA filter by pushing it straight into the cabinet, ensuring that it is correctly oriented with the gasket facing up on the exhaust side of the enclosure. The filter gasket is surrounded by a negative pressure plenum and the gasket will not leak. Be sure the filter fits properly.

CAUTION: The filter clamp bolts should only be tightened enough to ensure a proper seal at maximum tightness. The filter gasket should be compressed 50% or less.

8.Tighten the clamp bolts uniformly until the filter gasket is properly compressed against the frame. Inspect the seal thoroughly before proceeding.

9.Reinstall the filter access cover and the front panel.

10.Plug the enclosure in and have it recertified before use.

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Chapter 6: Maintaining Your Filtered Enclosure

Figure 6-1

HEPA Filter Changing Diagram & Filter Leak Test Diagram

43

Chapter 6: Maintaining Your Filtered Enclosure

HEPA Filter Leak Test

HEPA Purpose

After installing the new HEPA filter, the HEPA filter should be leak checked. This test is performed to determine the integrity of the HEPA filter, the filter housing, and the filter mounting frames. Leak testing is to be done by a qualified technician with calibrated equipment. Remove the top perforated exhaust cover by using a Phillips screwdriver to unfasten the (2) screws used to secure (2) clips. See Figure 6-1.The filter passes the leak test at .01% or better. Reference Leak Testing and Photometer scanning from the Institute of Environmental Services(IES-RP-CC001.3)

Apparatus

1.An aerosol photometer ATI model 2D, 2E, 2G or equivalent. Air Techniques Hamilton Associates inc. 11403 Cron Ridge Dr. Owings Mills, MD 21117

2.One aerosol generator of the Laskin nozzle(s) type. An aerosol of mineral oil or suitable liquid shall be created by flowing air through it. The compressed air supplied to the generator should be adjusted to a pressure of 10± 1 psig. during operation. Air Techniques Inc. Model TDA-4A or equal. One nozzle at 10 psig is (67.5 cfm x 100ug/l)/(Vol. of air), For the 2' at 90 fpm or 130 cfm, one nozzle @10 psig is 13,500/130 cfm = 52 ug/l. For the 3' at 90 fpm or 200 cfm, one nozzle @ 10 psig is 34 ug/l. For the 4' at 90 fpm or 265 cfm, one nozzle at 10 psig is 26 ug/l.

3.Mineral oil (Catalog #1491400).

4.Sampling Nozzle, Rectangular 1/2" x 3-1/4", Air Techniques, Inc.

Procedure

For the ATI 2G Photometer

1.Turn on the photometer and allow it to operate for a minimum of 5 minutes. Leave the valve in the “CLEAR” setting.

2.Press the “ENTER” keypad. Press the “REF” keypad.

The display will display “P1” for approximately 1 second, and then display a numerical value.

3.The display will display “P1” for approximately 1 second, and then display a numerical value.

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Chapter 6: Maintaining Your Filtered Enclosure

4.Using the “^” or “ν” keypads, respectively, increase or decrease the numerical value until it equals 52 (2'), 34 (3'). 26 (4') for the enclosure at 90 fpm inflow velocity.

5.Press the “ENTER” Keypad. The photometer will scan for 15 seconds, and then the “0” keypad will flash. Press the “Enter” keypad. The unit will scan for 5 seconds, the display will read “0000,” and the unit will sound a confirming tone.

6.Set the valve to “DOWNSTREAM.” Place the palm of your hand over the sampling port of the pistol. There should be a strong vacuum at this port. If the vacuum is weak, contact Air Techniques Hamilton Associates.

7.Turn the enclosure on and let it operate for a minimum of 5 minutes.

8.If necessary, adjust the speed control of the enclosure to maintain the following airflows at 90 fpm; 2' (130cfm), 3' (200 cfm, 4' (265 cfm.)

9.Position the aerosol generator discharge in the intake of the baffle inside the enclosure.

10.Start the aerosol generator (Pressure to be +/- 1 PSIG). Ensure that one Laskin nozzle is in the “open” position.

11.Allow the generator to operate for a minimum of 15 seconds. For all integral motorized impeller models, scan the downstream exhaust side of the HEPA filter by passing the sampling nozzle of the gun in slightly overlapping strokes over the entire surface the filter, with the sampling port not more than 1 inch from the surface of the filter media. Scan the entire periphery of the filter and the gasket between the filter frame and the enclosure frame. Scanning shall be done at a traverse rate of not more than 2 inches per second.

NOTE: For the Purifier Enclosures ducted to the outside, place the sampling nozzle in the center of the remote blower exhaust.

Acceptance

Aerosol penetration shall not exceed 0.01 percent measured by the photometer.

45

Chapter 6: Maintaining Your Filtered Enclosure

Setting the Inflow Face Velocity with the Speed Control Adjustment

1. Remove the front panel by loosening the (2) Phillips screws on top that secure the front panel.

2. The speed control is located on the electrical subassembly located behind the switched control panel and below the front panel. See Figure 6-1.

3. Adjust the speed control with a small Phillips screwdriver by turning the screw counterclockwise to increase blower speed or clockwise to decrease the blower speed. The speed control is very sensitive, so proceed with caution.

4. Measure the inflow velocity per the averaging technique

 

 

outlined in Chapter 3 and adjust the speed control slowly

 

 

for the desired speed. Allow the speed to stabilize and re-

 

 

measure the inflow velocity to confirm.

 

 

5. Replace the front panel and tighten the screws.

 

 

Calibrate and Operate the Airflow

 

 

Monitor

 

Options

Guardian Airflow Monitor (LED Monitor)

 

Refer to Figure 6-2for operation and calibration.

 

 

Labconco Airflow Monitor / Airflow Switch Operation

 

 

 

 

The Guardian Airflow Monitor (LED) consists of a circuit board

 

 

and an airflow switch. This switch indicates airflow as safe or low.

 

 

It does not provide an actual face velocity, but a small setscrew in

 

 

the back of the sensor can adjust the airflow level that it classifies

 

 

as “good/safe” or “low/alert.”

 

 

The circuit board provides power to the sensor and also contains a

 

 

“safe (green)” and “alert (red)” airflow LED indicators, as well as

 

 

a “SILENCE ALARM” button to quiet the audio alarm. When

 

 

first powered up, the PCB will light both red and green LED

 

 

indicators and sound the alarm to indicate it is working. After 5

 

 

seconds, the air monitor will indicate either good or bad airflow

 

 

based on what the connected airflow switch detects. For low

 

 

airflow, the unit will wait for 10 seconds of bad indications before

 

 

it sounds both the audio alarm and the red “alert” LED indicator. If

46

Chapter 6: Maintaining Your Filtered Enclosure

the “SILENCE ALARM” button is pressed, the audio alarm will be silenced, but the red “alert” LED will remain on. The alarm is silenced indefinitely unless an airflow change is detected. If safe airflow is later detected for 10 seconds, the green “safe” LED will be lit and the “alert” (red) LED will be shut off. At any time the airflow is safe/good, one can press the SILENCE ALARM test button and the audio alarm and the red LED will turn on as long as this button is held down. The PCB has also a two-pinconnector for use as an external output with isolated relay contacts that close when the red/alert LED is lit (low airflow). These relay contacts are not affected by the “SILENCE ALARM” button.

The PCB is mounted behind the front panel using standoffs and an appropriate label is used to highlight the “SILENCE ALARM” button with clear areas for the red and green LED’s. No holes to allow sound to be broadcast louder are necessary.

The PCB can be prepared as a factory special with an additional connector for the following external inputs, and having the following possible functions:

External Alarm allows an external signal to sound the alarm, such as a sash open switch, or a “fail” signal from the building airflow system.

Alarm Disable allows an external signal to prevent a “low” airflow alarm from occurring.

Night Setback allows an external signal to prevent a “low” airflow alarm from occurring (not any different from Alarm Disable above other than the terminology.)

Contact Labconco for ordering information on this special PCB.

Calibration

1.Ensure the flow switch and alarm circuit board are installed and operational.

2.Allow the enclosure to operate for at least two minutes.

3.If factory installed, the monitor will alarm at 60±10 fpm with the inflow velocity set at 90±10 fpm.

4.To change the factory setting, set the inflow velocity required by your Safety Officer to the desired alarm

condition using the speed control adjustment procedure outlined in Chapter 6.

Once the alarm condition is set, use a small screwdriver to turn the adjustment screw on the airflow switch counterclockwise (facing the screw) until the “low” airflow red LED lights and the audible flow alarm sounds.

47

Chapter 6: Maintaining Your Filtered Enclosure

5.Adjust the inflow velocity to the nominal operating point required by your Safety Officer.

6.Over time the HEPA filter will load and eventually slow the inflow velocity. Once the alarm condition is met, simply increase the speed control outlined in Chapter 6 or replace the HEPA filter if the speed control is maximized.

7.The table below lists typical alarm conditions based on normal operating conditions. Typical alarm conditions are set at face velocities of 10 to 30 feet per minute below the normal operating conditions due to supply air and exhaust air fluctuations, as well as room air cross drafts. Consult your Safety Officer for proper operating speeds.

Enclosure Operating In-Flow

Alarm Condition Set Point

Speed

Speed

100 ± 10 fpm

70-90fpm

90 ± 10 fpm

60-80fpm

80 ± 10 fpm

50-70fpm

70 ± 10 fpm

50-60fpm

60 ± 10 fpm

50 fpm

48

Chapter 6: Maintaining Your Filtered Enclosure

Figure 6-2

Guardian Airflow Monitor (LED) with Airflow Switch

49

Chapter 6: Maintaining Your Filtered Enclosure

Determination of When to Replace Odor Control Carbon Filters and How to Replace

The carbon filters MUST be replaced when anyone of the following two conditions are met:

1.The filtered enclosure outlet (exhaust) concentration approaches the inlet concentration, indicating filter saturation.

2.The odor in the work area becomes intolerable or the concentration of the chemical in the work area is greater than the TWA.

There are four means of determining when its time to change the carbon filters (not shown in the order of preference).

Odor - A person’s sensitivity to odor, tolerance of odor and their comfort level under odoriferous conditions vary with the individual. While odor is an indicator that chemicals are passing through the carbon filter, several points need to be understood:

Odor within the room is not necessarily an indication of saturation or hazardous exposure concentrations.

Odor can be used as a prompt to sample the chemical concentration on the exit side of the carbon filter.

Organic chemicals approved for use in the filtered enclosure have odors that are detectable before reaching the time weighted exposure limits.

Detection Tubes - Color change indicators can be used to measure the concentration of the chemical at the exit side of the carbon filter or in the outlet exhaust. A kit including syringe pump and flexible tubing can be purchased as an accessory from Labconco (Catalog # 6924900). Labconco Customer Service Representatives are supplied with detector tube catalog numbers, as well as telephone numbers to direct you to where to purchase these items.

For Organic, Formaldehyde and Ammonia, chemical specific detector tubes should be purchased when installing fresh filters. Each kit contains instructions on how many strokes of the syringe are required to obtain the stated sensitivity. The sampling syringe is connected to the filtered enclosure exhaust. Connect the syringe to the detector tube while the system is running and pull the air through the tube with the syringe. Each stroke of the

50

Chapter 6: Maintaining Your Filtered Enclosure

syringe represents a 100-mlsample and corresponds to the number of strokes necessary to give the indicated color changes. Due to the wide variety of organics and varying TWA’s, it is recommended that specific detector tubes be purchased directly from Sensidyne, Draeger or your laboratory supply dealer. Alternate detector pumps can also be purchased from your laboratory supply dealers. The vast majority of detector tubes available start measuring at the TWA. When a user observes a color change in the tube, they should replace the filter immediately. If no detector tube for your specific chemical is available, other means of detection must be used.

Time - For applications that have very consistent inlet concentrations and operating time, “Time” can be used to anticipate saturation or TWA levels based on prior experience. However, this does not replace the need for sampling. Consult Labconco technical specialist for an estimate of carbon filter life based on chemical usage. Detector tubes, or analytical instrumentation shouldalways be used to determine concentrations in the carbon filter. It is recommended that the carbon filters be checked with detector tubes or other means at intervals of 20% of the total estimated filter life. The exception to the 20% recommendation is formaldehyde and any carcinogen or suspected carcinogen. These more hazardous chemicals must be checked at least every 10% of the total estimated time.

Analytical Instrumentation - This is the most accurate means of measuring concentrations ofany chemical. It is the method of choice when no detector tubes are available or the tubes are not sensitive enough to measure at the TWA concentration for the chemical. This method is also to be used to determine saturation when the chemical concentration is below the measurement range of detector tubes.

Odor Control Carbon Filter Replacement Procedure – See

Figures 4-1and4-2for Carbon Filter Replacement.

1.The carbon filters are replaced by first removing the two screws, which hold the clips to secure the upper diffuser screen. Remove the screws, clips and upper diffuser screen.

2.Remove the carbon filter in a careful way to avoid the flow switch and HEPA filter.

51

Chapter 6: Maintaining Your Filtered Enclosure

3.Re-installthe new carbon filter with the gasket down. Replace the upper diffuser screen, clips and two screws.

4.The weight of the carbon filter with the gasket down will compress the gasket.

Calculating Odor Control Carbon Filter Life

Labconco developed a modeling program to estimate the filter life for typical carbon filters. Since filter life is dependent on the chemical used, the airflow, filter size, and the dwell time, refer to the Chemical Guide for the Paramount® Filtered Enclosure. The estimated life for Odor Control carbon filters for the filtered enclosures is conservatively calculated at 50% or half of the published values for the Paramount in the Chemical Guide. For example, if you use isopropyl alcohol to disinfect and use approximately 100 ml per week during 2 hours of use per day then follow these steps to calculate the concentration in parts per million (ppm).

Steps for Calculating PPM and Filter Life

1.Determine the amount of the proposed chemical lost to evaporation over a given amount of time. For example, if you use isopropyl alcohol and lose approximately 100 ml per week during 2 hours of use per day.

2.Convert the amount lost into ml/min. For this example:

100 ml X

1 week X

10 hours =

100 ml lost

=.17 ml/min

week

10 hours use

600 minutes

600 minutes

 

3.Convert ml/min to PPM by multiplying ml/min by the conversion factor found in the second to the last column on the right. For isopropyl alcohol .17 x 41 = 7.0 PPM.

4.Find the PPM value on the chart that comes closest to the value you just calculated in step #3. In this example, round up to 10 PPM, which is close to the calculated 7.0. We may approximate the filter life to be around 155 hours of actual use, but use 50% of this for the Odor Control filters or 78 hours.

5.Insert the estimated filter life into the estimated usage to determine how long filters will last.

78 hours filter life

=7.8 weeks before filter saturation

10 hours per week use

 

52

Chapter 6: Maintaining Your Filtered Enclosure

Formaldehyde only

For formaldehyde, use 10% of the impregnated carbon weight. Formalin is 37% formaldehyde by weight. The density of formalin is 1.08 g/ml.

 

 

Pounds of

Adsorbed weight of

Adsorption

Model

 

Formasorb

Formaldehyde

Volume of

Size

Filter Size

Carbon

 

Formalin

2'

18 x 18 x 1

7.0

0.7 lbs./318g

796 ml

3'

30 x 18 x 1

12.0

1.2 lbs./545g

1364 ml

4'

42 x 18 x 1

16.8

1.68 lbs./763g

1910 ml

Ammonia only

For ammonia, use 10% of the impregnated carbon weight. Assume use of a 50% solution of ammonia for these calculations.

 

 

Pounds of

 

Adsorbed

 

 

Ammonasorb

 

Volume of 50%

Model

 

II Carbon

Adsorbed weight of

Ammonia

Size

Filter Size

 

Ammonia

Solution

2'

18 x 18 x 1

9.2

0.92 lbs./418g

836 ml

3'

30 x 18 x 1

13.5

1.35 lbs./613g

1226 ml

4'

42 x 18 x 1

18.9

1.89 lbs./859g

1718 ml

Initial Certification

The filtered enclosure has been certified at the factory for an inflow velocity of 90±10 fpm along with the HEPA Filter Leak Test. The filtered enclosure should be certified for the proper inflow velocity required by your Safety Officer. It is also a conservative recommendation to perform the HEPA Filter Leak Test again should there be any damage caused during transport.

Re-Certification

Under normal operating conditions, the enclosure should be recertified at least annually if serviced. The certifier should perform the following tests.

Inflow Velocity Test

HEPA Filter Leak Test

In addition, the following tests should also be performed at the user’s discretion:

53

Chapter 6: Maintaining Your Filtered Enclosure

Measure of Line Voltage and Current

Smoke Test to determine proper airflow patterns

Lighting Intensity Test (when appropriate)

Noise Level Test (when appropriate)

Vibration Test (when appropriate)

Fluorescent Light Replacement

1.Disconnect the power.

2.Locate the small light reflector located under the control panel shown in Figure 6-2.

3.Remove the light reflector support by removing two Phillips screws on the bottom of the light reflector.

4.Rotate and remove the old fluorescent lamp.

5.Reinstall the new fluorescent lamp and light reflector in reverse order.

6.Power the unit up and try the new fluorescent lamp.

UV Light Replacement

1.Disconnect the power.

2.Locate the UV lamp located inside the enclosure.

3.Surface decontaminate the old UV light before handling it.

4.Rotate and remove the old UV lamp.

5.Reinstall the new UV lamp.

6.Power the unit up and try the new UV lamp.

Motorized Impeller Replacement

The motorized impeller must be replaced as a complete unit. When the motorized impeller is replaced, the capacitor may also be replaced. See Appendix A for Replacement Parts Diagram. See Figure 6-5for an isometric view of the motorized impeller plenum assembly. The HEPA filter rests on top of the motorized impeller assembly.

1.Wear appropriate personal protective equipment to decontaminate the filtered enclosure and then unplug from the electrical outlet.

2.Remove HEPA filter per the HEPA filter removal procedure outlined in this chapter.

3.Consult the wiring diagram in Appendix C of the manual and disconnect all the wires of the motorized impeller. Be sure to connect wires on the new motor in the same way the old motor was wired.

54

Chapter 6: Maintaining Your Filtered Enclosure

4.Remove four screws in the motor bracket that hold motor bracket to the supports with the vibration isolation mounts. Remove the motor and bracket.

!WARNING: High-speedblower. Never operate impeller with housing off.

5.Replace the capacitor with a new one of equal voltage and capacity.

6.Reassemble the new motorized impeller by reversing the assembly steps.

Figure 6-3

Motorized Impeller Replacement

55

Chapter 6: Maintaining Your Filtered Enclosure

Speed Control Replacement

1.Remove the bracket that the speed control is attached to. See Figures 6-1,and6-2.

2.Remove the two screws holding the speed control using a Phillips screwdriver. Refer to Appendix A for Replacement Parts Diagram.

3.Disconnect all wires leading to the speed control. Connect wires on new speed control in the same position as the old speed control.

4.Reassemble to the system in the same position and with the same screws that were removed earlier.

56

CHAPTER7

ACCESSORIZING AND

MODIFYINGYOUR

FILTEREDENCLOSURE

There are several ways to accessorize and modify the filtered enclosure for your individual requirements. These include the addition of accessory work surfaces, airflow monitors; exhaust transition kits, remote blowers, exhaust dampers, filters, storage cabinets and utility shelf kit.

1. Work Surfaces

An optional dished work surface is available to attach to the filtered enclosure.

Dished work surfaces are contoured to fit the dimensions of the filtered enclosures to contain spills. Epoxy is chemical resistant. See Figure 3-1for installation.

Catalog #

Description

Dimensions (W x D x H)

3909900

Black, 2-footwide

24" x 29" x 1"

3909901

Black, 3-footwide

36" x 29" x 1"

3909902

Black, 4-footwide

48" x 29" x 1"

3909903

Gray, 2-footwide

24" x 29" x 1"

3909904

Gray, 3-footwide

36" x 29" x 1"

3909905

Gray, 4-footwide

48" x 29" x 1"

57

Chapter 7: Accessorizing and Modifying Your Filtered Enclosure

2.Guardian™ LED Airflow Monitor

The Guardian LED Airflow Monitor allows you to continuously monitor airflow through the enclosure. The Guardian LED monitor can be placed on any Purifier enclosure.

Description

Order Part #’s

Individual Parts Required

Guardian Airflow Monitor

 

 

115V or 230V

 

 

1 ea.

3811500

Airflow Monitor Printed Circuit Board

1 ea.

3910700

Airflow Sensor w/ Connector

2 ea.

1885405

#6-32x .31" Phillips machine screw to

 

mount Airflow Monitor Circuit Board

 

 

2 ea.

1891808

#6-32x .50" Phillips thread cutting screw to

 

mount Airflow Sensor

 

 

 

 

 

3. Exhaust Transition Kits for Ducting to Outside

Exhaust transition connects to the enclosures with integral motorized impellers from the top so the duct can be routed up and outside the building. The transition is available for 6" PVC duct. The transition should be sealed to the top of the enclosure with silicone sealant to prevent leaks. See Figure 3-3.

Order the following:

Part

Description

3924400

2' Exhaust Transition Kit

3924401

3' Exhaust Transition Kit

3924402

4' Exhaust Transition Kit

4. Remote Blowers

For Purifier Class I Enclosure

The remote blower has 1/4 hp direct drive motor and corrosion-resistantepoxy-coatedsteel housing and wheel with blower inlet of 5.94" OD. sized to accept 6" nominal PVC duct. Includes a blower transition adapter outlet for 6" PVC duct with integral back draft damper.

CFM @ Static Pressure-Inchesof H2O

S.P.

0.0"

0.125"

0.25"

0.50"

0.75"

0.87"

CFM

595

560

515

420

300

167

58

Chapter 7: Accessorizing and Modifying Your Filtered Enclosure

Used With

Catalog #

Description

Shipping Wt. (lbs./kg.)

 

 

3716000

Remote Blower, 115V, 60 Hz. 4.4

35/16

 

Purifier Class I

 

amps

 

 

3716001

Remote Blower, 115/230V, 50 Hz,

35/15

 

 

 

 

 

 

5.6/2.8 amps

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Figure 7-1

For Purifier HEPA Filtered Enclosure

Has a 1/4 hp direct drive motor and corrosion-resistantepoxycoated steel housing and wheel with blower inlet of 6.00" ID. Outlet dimensions are 4.25" x 7.38" OD.

CFM @ Static Pressure-Inchesof H2O

 

S.P.

0.0"

 

0.125"

0.25"

0.50"

 

0.75"

0.87"

 

CFM

595

 

560

515

420

 

300

167

 

 

 

 

 

 

 

 

 

 

 

Catalog #

Description

 

 

 

 

Shipping Wt.

 

 

 

 

 

(lbs./kg.)

 

 

 

 

 

 

 

 

 

Used with Purifier

4863500

Remote Blower, 115 V, 60 Hz. 4.4 amps

 

35/16

4863501

Remote Blower, 115/230 V, 50 Hz, 5.6/2.8 amps

35/16

HEPA Filtered

 

Explosion-ProofRemote Blower, 115 V, 60 Hz,

 

Enclosure

7053501

40/18

4.4 amps

 

 

 

 

 

 

 

 

 

 

 

 

 

59

Chapter 7: Accessorizing and Modifying Your Filtered Enclosure

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

5.

Exhaust Dampers

 

 

Exhaust dampers allow adjustments required to maintain

 

 

proper airflow for roof-mountedblowers or house exhaust

 

 

systems.

 

 

 

 

 

 

 

 

 

Catalog #

Description

 

 

3924000

6" Epoxy Coated Steel In-Lineadjustable damper

 

 

4724200

6" PVC In-lineadjustable damper

 

6.

Accessory Filters

HEPA Filter

HEPA Filter is 99.99% efficient on particles 0.3 micron.

Width

Catalog #

Size

2'

3707900

18" x 18" x 3.31"

3'

3707901

30" x 18" x 3.31"

4'

3707902

42" x 18" x 3.31"

Odor Control Carbon Filter

Provides granular activated carbon or impregnated carbon used for Odor Control applications on integral motorized impeller models exhausted back into the room.

Filter Classification and Size

Catalog #

X Pounds

2' Organic 18 x 18 x 1

3937200

5.5 lbs. activated

3' Organic 30 x 18 x 1

3937300

9.2 lbs. activated

4' Organic 42 x 18 x 1

3937400

12.9 lbs. activated

 

 

 

2' Formaldehyde 18 x 18 x 1

3937201

7.0 lbs impregnated

3' Formaldehyde 30 x 18 x 1

3937301

12.0 lbs. impregnated

4' Formaldehyde 42 x 18 x 1

3937401

16.8 lbs. impregnated

 

 

 

2' Ammonia & Amines 18 x 18 x 1

3937202

8.0 lbs. impregnated

3' Ammonia & Amines 30 x 18 x 1

3937302

13.5 lbs. impregnated

4' Ammonia & Amines 42 x 18 x 1

3937402

18.9 lbs. impregnated

60

Chapter 7: Accessorizing and Modifying Your Filtered Enclosure

7.

Storage Cabinets

SOLVENT

 

 

ACID

 

 

 

 

 

 

 

Size/Description

 

Dual Doors

Right Hinge

 

Left Hinge

Dual Doors

Right Hinge

 

Left Hinge

48"

 

9902000

-

 

-

9901000

-

 

-

36"

 

9902100

-

 

-

9901100

-

 

-

30"

 

9902200

-

 

-

9901200

-

 

-

24"

 

-

9902300

 

9902400

-

9901300

 

9901500

18"

 

-

-

 

-

-

9901400

 

9901600

12"

 

-

-

 

-

-

-

 

-

48" w/Self-ClosingDoors

9903000

-

 

-

-

-

 

-

36" w/Self-ClosingDoors

9903100

-

 

-

-

-

 

-

30" w/Self-ClosingDoors

9903200

-

 

-

-

-

 

-

24" w/Self-ClosingDoors

-

9903300

 

9903400

-

-

 

-

24" ADA

 

-

9906000

 

9906100

-

9905000

 

9905200

24" ADA w/Self-Closing

 

-

9906200

 

9906300

-

-

 

-

Doors

 

 

 

 

 

 

 

 

 

18" ADA

 

-

-

 

-

-

9905100

 

9905300

12" ADA

 

-

-

 

-

-

-

 

-

 

 

 

 

 

 

 

 

 

 

 

 

STANDARD BASE

 

VACUUM PUMP

 

Size/Description

 

Dual Doors

Right Hinge

 

Left Hinge

Dual Doors

Right Hinge

 

Left Hinge

48"

 

9900000

-

 

-

-

-

 

-

36"

 

9900100

-

 

-

-

-

 

-

30"

 

9900200

-

 

-

-

-

 

-

24"

 

-

9900300

 

9900600

-

-

 

-

18"

 

-

9900400

 

9900700

-

9907000

 

9907100

12"

 

-

9900500

 

9900800

-

-

 

-

48" w/Self-ClosingDoors

-

-

 

-

-

-

 

-

36" w/Self-ClosingDoors

-

-

 

-

-

-

 

-

30" w/Self-ClosingDoors

-

-

 

-

-

-

 

-

24" w/Self-ClosingDoors

-

-

 

-

-

-

 

-

24" ADA

 

-

9904000

 

9904300

-

-

 

-

24" ADA w/Self-Closing

 

-

-

 

-

-

-

 

-

Doors

 

 

 

 

 

 

 

 

 

18" ADA

 

-

9904100

 

9904400

-

-

 

-

12" ADA

 

-

9904200

 

9904500

-

-

 

-

8.

Utility Shelf Kit

 

 

 

 

 

 

 

Labconco offers a utility shelf kit part number 3925000 consisting of three shelves to hold items inside the enclosure. One shelf may be used for the printer, one shelf for spatulas and weigh brushes, and one miscellaneous shelf. The shelves hang from slots in the rear baffle.

61

Chapter 7: Accessorizing and Modifying Your Filtered Enclosure

62

CHAPTER8

TROUBLESHOOTING AND

SERVICEROPERATING

LOG

Refer to the following table if your filtered enclosure fails to operate properly. If the suggested corrective actions do not solve your problem, contact Labconco for additional assistance.

PROBLEM

CAUSE

CORRECTIVE ACTION

 

 

 

Contaminants outside

Improper user

See “Certifying the Enclosure” Chapter

of enclosure.

techniques for the

3 and “Safety Precautions” Chapter 4

 

enclosure.

sections in the manual. (Ref. Appendix

 

 

D).

 

Restriction of the

Remove obstruction to ensure that all

 

baffle air slots or

air slots and the exhaust outlet are

 

blockage of the

unobstructed.

 

exhaust outlet.

 

 

External factors are

See “Location Requirements” Chapter

 

disrupting the

2, “Certifying the Enclosure” Chapter

 

enclosure airflow

3, and “Safety Precautions” Chapter 4

 

patterns or acting as a

sections of this manual. (Ref. Appendix

 

source of

D).

 

contamination.

 

 

Enclosure has

Have enclosure certified and check

 

improper face

exhaust system. Check HEPA filters for

 

velocity.

loading. Adjust enclosure speed

 

 

control. Enclosure should have an

 

 

average face velocity of 60-100fpm for

 

 

weighing procedures and 75-105fpm

 

 

for biohazardous procedures.

Chapter 8: Troubleshooting

 

PROBLEM

 

CAUSE

CORRECTIVE ACTION

 

Blower won’t

 

Unit not plugged

Plug the enclosure into appropriate

 

operate.

 

into outlet.

electrical service.

 

 

 

Circuit breaker(s)

Reset circuit breaker.

 

 

 

or Ground Fault

 

 

 

 

Interrupter.

 

 

 

 

Blower wiring is

Inspect blower wiring.

 

 

 

disconnected.

 

 

 

 

Blower switch is

Replace switch.

 

 

 

defective.

 

 

 

 

Motorized impeller

Replace motorized impeller or blower.

 

 

 

or blower is

See Chapter 6.

 

 

 

defective.

 

 

Low face velocity

 

Enclosure sash not

Close sash to the lowest position.

 

or poor

 

closed.

 

 

containment of

 

 

 

 

contaminants.

 

 

 

 

 

 

HEPA filter

Replace HEPA filter or increase speed.

 

 

 

clogged.

 

 

 

 

Carbon filter loaded

Replace carbon filter.

 

 

 

with chemicals.

 

 

Blower and lights

 

Unit not plugged

Plug enclosure into appropriate

 

won’t operate.

 

into outlet.

electrical service.

 

 

 

Circuit breaker(s)

Reset or replace circuit breaker.

 

Lights do not work.

 

tripped.

 

 

 

Lamp not installed

Inspect lamp installation.

 

 

 

properly.

 

 

 

 

Lamp wiring

Inspect lamp wiring.

 

 

 

disconnected.

 

 

 

 

Defective lamp.

Replace lamp.

 

 

 

Light switch is

Replace light switch.

 

 

 

defective.

 

 

 

 

Defective electronic

Replace ballast.

 

 

 

ballast.

 

 

Airflow monitor

 

No power.

Power cable to circuit board is

 

malfunction.

 

No lights.

disconnected and needs to be

 

 

 

No display.

connected. Sensor cable needs to be

 

 

 

 

connected. Verify that all airflow

 

 

 

 

monitor interface cables are connected.

 

 

 

 

Check fuses on enclosure.

 

 

 

No audible alarm.

Alarm has been temporarily silenced

 

 

 

 

using “SILENCE ALARM”.

64

 

 

 

 

Chapter 8: Troubleshooting

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

PROBLEM

CAUSE

CORRECTIVE ACTION

 

 

Airflow Monitor

Wrong alarm set

Airflow monitor was not properly

 

 

 

Malfunction

point.

adjusted. Repeat calibration steps

 

 

(Continued)

 

 

outlined in this manual in Chapter 6.

 

 

 

Constant audible

Check airflow and calibration of

 

 

 

 

alarm.

airflow monitor. See Chapter 6.

 

 

 

Continuous alarm.

Check the face velocity of the

 

 

 

 

 

 

enclosure as the airflow of the

 

 

 

 

 

system may have changed. If

 

 

 

 

 

incorrect, adjust the speed control to

 

 

 

 

 

increase face velocity. The HEPA

 

 

 

 

 

filter may have become loaded. If

 

 

 

 

 

face velocity is correct, calibrate the

 

 

 

 

 

airflow monitor outlined in this

 

 

 

 

 

manual in Chapter 6.

 

 

 

Monitor alarms; air

Lightly clean the airway with clean

 

 

 

 

way to airflow

air. Be careful not to touch sensitive

 

 

 

monitor sensor is

electrical components.

 

 

 

blocked by insects,

 

 

 

 

 

dust or debris.

 

 

 

 

 

Audible disable

An alarm condition must be

 

 

 

 

will not stay

continuously present before the

 

 

 

operational.

audible alarm can be silenced. If

 

 

 

 

 

flow conditions fluctuate near the

 

 

 

 

 

alarm set point, the airflow monitor

 

 

 

 

 

will automatically reset it. Action

 

 

 

 

 

should be taken to bring the

 

 

 

 

 

enclosure airflow into proper

 

 

 

 

 

operating parameters or adjust the

 

 

 

 

 

alarm set point lower.

 

65

Chapter 8: Troubleshooting

 

 

 

Service Operating Record Log

 

 

 

Customer Name

 

Model Number

 

 

 

 

Date Installed

 

Serial Number

 

 

 

Unit Location

 

 

 

 

 

 

 

 

 

 

 

 

Date

 

Comments

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

66

APPENDIXA

FILTEREDENCLOSURE

COMPONENTSAND

REPLACEMENTPARTS

The components that are available for your filtered enclosure are listed. The parts shown are the most commonly requested. If other parts are required, please contact Product Service.

Appendix A: Replacement Parts

Item

Qty.

Part Number

Description

1

1

3911000

Glass, Side

2A

1

3911900

Glass, Sash 2'

2B

1

3911901

Glass, Sash 3'

2C

1

3911902

Glass, Sash 4'

3A

1

3911800

Sash Assembly, 2'

3B

1

3911801

Sash Assembly, 3'

3C

1

3911802

Sash Assembly, 4'

4A

1

3905600

Lower Air Foil, 2'

4B

1

3905601

Lower Air Foil, 3'

4C

1

3905602

Lower Air Foil, 4'

5A

1

3907400

Bracket, Air Foil R.H.

5B

1

3907401

Bracket, Air Foil L.H.

6A

1

3907500

Stop, Sash R.H.

6B

1

3907501

Stop, Sash L.H.

7A

1

6916500

Latch, Sash

7B

1

3906700

Bracket, Latch

7C

1

1927403

Spring, Compression Latch

7D

2

1893206

Screw, 8-32x .38" Lg. Phillips Pan S.S. Type F

9A

2

1889316

Screw, 10-24x 1.00 PH Phil S.S.

9B

2

1912108

Washer, Plastic .194 ID. x .380 OD

9C

2

7868402

Bushing, Spacer .31 x .63

10

1.9 Ft

6913700

Wiper, Sash Seal

11A

2

1936800

Bushing, Heyco closed 1.50 Dia.

11B

2

1934601

Bushing, Heyco w/ Flex Shutter 1.50 Dia.

12A

1

3915401

Side Air Foil

12B

2

1889912

Screw, #6-32x .75" Oval Hd. Type F (S.S.)

13A

1

3907700

Reflector, Light 2'

13B

1

3907701

Reflector, Light 3'

13C

1

3907702

Reflector, Light 4'

13D

2

1889308

Screw, Mach. 10-24x .50 PH Phillips

14A

1

3708300

Lamp, Fluorescent 2' Enclosure (F15T8-SP35)

14B

1

9721902

Lamp, Fluorescent 3' Enclosure (F17T8)

14C

1

9721901

Lamp, Fluorescent 4' Enclosure (F25T8)

15A

1

1270100

Lamp, UV 2' or 3' Enclosure (G15T8)

15B

1

1271300

Lamp, UV 4' enclosure (G30T8)

16A

1

3911700

Bracket, Right Reflector Support

16B

1

3911701

Bracket, Left Reflector Support

16C

4

1893108

Screw, #8 x 1/2 AB Hex Washer Head

68

Appendix A: Replacement Parts

Item

Qty.

Part Number

Description

17A

1

3918500

Wiring Harness Main, 115V

17B

1

3918600

Wiring Harness Main, 230V

18A

1

3811500

Guardian Airflow Monitor Printed Circuit Board

18B

2

1885405

Screw Mach #6-32x .31 PH Phil. S.S.

19A

1

3926600

Label, Front Class I

19B

1

3926700

Label, Front HEPA

20A

1

3910700

Airflow Switch

20B

2

1891808

Screw, Thread Cutting #6-32x .50 PH

21A

1

3906200

Cover Plate for HEPA 2'

21B

1

3906201

Cover Plate for HEPA 3'

21C

1

3906202

Cover Plate for HEPA 4'

21D

Multiple

1905617

Nut, KEPS 10-24

22A

1 or 2

1307000

Switch, Rocker (2 position)

22B

1

1306300

Switch, Rocker (3 position) – UV Light Only

23A

1 or 2

3904800

Front Panel, 2'

23B

1

3904801

Front Panel, 3'

23C

1

3904802

Front Panel, 4'

23D

1

4472400

Screw, Thread cut 10-24x .50 Type F PH Phillips

24A

1

3916500

Impeller, Motorized 2' or 3' Enclosure, 115V

24B

1

3916501

Impeller, Motorized 2' or 3' Enclosure, 230V

24C

1

3903300

Impeller, Motorized 4', 115V

24D

1

3903301

Impeller, Motorized 4', 230V

24E

4

1601800

Vibration Isolator Mount

25A

1

1306900

Capacitor, 2', 115V 10 MFD

25B

1

1306900

Capacitor, 3', 115V 10 MFD

25C

1

1306600

Capacitor, 4', 115V 15 MFD

25D

2

1306800

Capacitor, 2', 230V 4 MFD (effective 2MFD)

25E

2

1306800

Capacitor, 3', 230V 4 MFD (effective 2MFD)

25F

1

1306800

Capacitor, 4', 230V 4 MFD

26A

1

1235400

Ballast, Fluorescent 2', 115V

26B

1

1235500

Ballast, Fluorescent 2', 230V

26C

1

1294000

Ballast, Fluorescent 3' or 4', 115V or 230V

27A

1

1235400

Ballast, UV 2' or 3', 115V

27B

1

1235500

Ballast, UV 2' or 3', 230V

27C

1

1233400

Ballast, UV 4', 115V

27D

1

1233600

Ballast, UV 4', 230V

28A

1

1325500

Speed Control, 115V

28B

1

1325501

Speed Control, 230V

29A

1

3910800

Upper Diffuser Screen 2'

29B

1

3910801

Upper Diffuser Screen 3'

29C

1

3910802

Upper Diffuser Screen 4'

30A

2

3916700

Clip, Upper Diffuser

69

Appendix A: Replacement Parts

Item

Qty.

Part Number

Description

30B

2

1893708

Screw, #10 x .50 PH Phil. Thd. Forming

31A

2

1881196

Capscrew, 3/8 –16x 6.0 Lg. Hx. Hd.

31B

2

1924403

Sealing Washer 3/8

32A

1

3707900

HEPA Filter (2') 18 x 18 x 3.32 w/ Gasket

32B

1

3707901

HEPA Filter (3') 30 x 18 x 3.32 w/ Gasket

32C

1

3707902

HEPA Filter (4') 42 x 18 x 3.32 w/ Gasket

33A

1

3937200

2' Odor Control Organic Vapor Carbon Filter

33B

1

3937201

2' Odor Control Formaldehyde Carbon Filter

33C

1

3937202

2' Odor Control Ammonia Carbon Filter

33D

1

3937300

3' Odor Control Organic Vapor Carbon Filter

33E

1

3937301

3' Odor Control Formaldehyde Carbon Filter

33F

1

3937302

3' Odor Control Ammonia Carbon Filter

33G

1

3937400

4' Odor Control Organic Vapor Carbon Filter

33H

1

3937401

4' Odor Control Formaldehyde Carbon Filter

33I

1

3937402

4' Odor Control Ammonia Carbon Filter

34

1

7907802

Digital Monitor Outlet, 115V only, 230V

 

 

 

connected at laboratory outlet location.

35A

1

1333800

IEC Power Inlet

35B

1

1305800

Power Cord, 115V, Right Angle

35C

1

1334200

Power Cord, 230V, Right Angle

36A

1 - (115V)

1327200

Circuit Breaker, 3 AMP

36B

2 - (230V)

1327205

Circuit Breaker, 2 AMP

40A

1

1952500

Gauge, Pressure Mini Helic II

40B

1

3667400

Bracket, Gauge

40C

2

1905617

Nut, #10-24KEPS

40D

2

1551700

Tubing, 3/16" ID x 5/16" OD, PBC 7.00" Lg.

40E

2, 1" Long

3788200

Snubber, Filter Pressure Gauge

70

Appendix A: Replacement Parts

Use for Purifier Class I and Purifier HEPA Filtered Enclosures

18

40 19

13

14

7

21

20

17

25 27

22

24

26

 

23

 

28

29

30

33

Placed on ledge above HEPA filter

31

35

36

32

16

9

12

11

5

4

1

3

10

2 6

71

APPENDIXB

DIMENSIONS AND

EXHAUSTOPTIONS

See the following dimensions and exhaust options for all the Purifier Filtered Enclosures.

72

Appendix B: Dimensions and Exhaust Options

Figure B-1

Purifier Class I and Purifier HEPA Filtered Enclosure

73

Appendix B: Dimensions and Exhaust Options

Figure B-2

Exhaust Duct Connection Kit (Optional Installation on Integral Blower Models can be installed on Purifier Class I and Purifier

HEPA Filtered Enclosure)

74

APPENDIXC

FILTERED

ENCLOSURE

SPECIFICATIONS

This Appendix contains technical information about all the Purifier HEPA Filtered Enclosures including electrical specifications, environmental operating conditions and wiring diagrams.

3 Amps, 115V or 2 Amps, 230V, 50/60 Hz, XPert Filtered Balance System and XPert Filtered Balance Station.

Environmental Conditions

Indoor use only.

Maximum altitude: 6562 feet (2000 meters).

Ambient temperature range: 41° to 104°F (5° to 40°C).

Maximum relative humidity: 80% for temperatures up to 88°F (31°C), decreasing linearly to 50% relative humidity at 104°F (40°C).

Main supply voltage fluctuations not to exceed ±10% of the nominal voltage.

Transient over-voltagesaccording to Installation Categories II(Over-voltageCategories per IEC 1010). Temporary voltage spikes on the AC input line that may be as high as 1500V for 115V models and 2500V for 230V models are allowed.

75

Appendix C: Filtered Enclosure Specifications

Used in an environment of Pollution degrees 2 (i.e., where normally only non-conductiveatmospheres are present). Occasionally, however, a temporary conductivity caused by condensation must be expected, in accordance with IEC 664.

76

Appendix C: Filtered Enclosure Specifications

C-1

77

Appendix C: Filtered Enclosure Specifications

C-2

78

Appendix C: Filtered Enclosure Specifications

C-3

79

Appendix C: Filtered Enclosure Specifications

C-4

80

PPENDIX

D

 

 

 

A

 

 

 

 

 

UICK

HART

OR

 

 

Q

 

C

 

F

 

 

HE

ILTERED

 

 

 

T

F

 

 

 

 

 

NCLOSURES

 

 

 

E

 

 

 

 

 

 

Model Size

 

 

 

2'

3'

4'

Sash Height from Work Surface (inches)

9.44

9.44

9.44

Total Open Area with Bypass (sq. ft.)

1.45

2.20

2.95

Exhaust Airflow Volume at 90fpm (CFM)

130

200

265

Alarm Airflow Volume at 60 fpm (CFM)

85

130

175

Initial Mag. Gauge Pressure Reading (inches H2O)

0.20 to .045

0.15 to 0.40

0.10 to 0.35

Number of Laskin Nozzles needed at 10 psig

1

1

1

Exhaust HEPA Filter w/ Gasket Dimensions (inches)

18x18x3.31

30x18x3.31

42x18x3.31

 

*Motor Horsepower (HP)

0.13

0.13

0.30

 

*Capacitor (MFD, Volts)

10MFD, 115V

10MFD, 115V

15MFD, 115V

 

 

 

 

(2) 4MFD, 230V

(2) 4MFD, 230V

4MFD, 230V

 

 

Fluorescent Lights

F15T8-SP35

F17T8

F25T8

 

UV Light on Class I only

G15T8

G15T8

G30T8

To determine the actual face velocity at the sash opening, airflow velocity readings will need to be taken. This should be done across the sash opening of the enclosure in accordance with the Industrial Ventilation Manual. (See Appendix E) The “average face velocity” is achieved by taking readings in two rows across the enclosure with the readings 6" from the ends and evenly spaced every 12"; the first row is 3" down from the upper sash foil and the second row is 3" up from the work surface. A total of four readings will be taken for the 2' enclosure, six readings taken for the 3' enclosure and eight readings taken for the 4' enclosure and then averaged.

81

APPENDIXE

REFERENCES ON

VENTILATION,

SAFETY,

OCCUPATIONAL

HAZARDS,

BIOSAFETY AND

DECONTAMINATION

Many excellent reference texts and booklets are currently available. The following is a brief listing:

Laboratory Ventilation Standards

Federal Register 29 CFR Part 1910

Non-mandatoryrecommendations from “Prudent

Practices.”

Fume hoods should have a continuous monitoring device

Face velocities should be between 60-100linear feet per minute (lfpm)

Average 2.5 linear feet of hood space per person

82

Appendix E: References

Occupational Health and Safety U.S. Department of Labor

200 Constitution Avenue N.W. Washington, DC 20210

(202) 523-1452www.osha.gov

Industrial Ventilation-ACGIH

Fume hood face velocities between 60-100lfpm

Maximum of 125 lfpm for radioisotope hoods

Duct velocities of 1000-2000fpm for vapors, gasses and smoke

Stack discharge height 1.3-2.0x building height

Well designed fume hood containment loss, <0.10 ppm

Industrial Ventilation, A Manual of Recommended

Practice.

24th Edition, 2001

American Conference of Governmental Industrial Hygienists

1330 Kemper Meadow drive Cincinnati, OH 45240-1634(513)742-2020www.acgih.org

ASHRAE 110-1995Method of Testing Performance of

Fume Hoods

Evaluates fume hood’s containment characteristics

Three part test: Smoke generation, Face velocity profile, Tracer gas release @ 4 liters per minute

Rated As Manufactured (AM), As Installed (AI) and As Used (AU)

American Society of Heating, Refrigerating, and Air Conditioning Engineers

1791 Tullie Circle N.E. Atlanta, GA 30329 (404) 636-8400www.ashrae.org

ANSI Z9.5-1993Laboratory Standard

Covers entire laboratory ventilation system.

Vertical stack discharge @ 2000-3000fpm

New and remodeled hoods shall have a monitoring device

Ductless hoods should only be used with non-hazardousmaterials

Fume hood face velocities between 80 – 120 fpm

83

Appendix E: References

American Industrial Hygiene Association 2700 Prosperity Avenue, Suite 250 Fairfax, VA 22031

(703) 849-8888www.aiha.org

SEFA 1-2002

Fume hood face velocities based on toxicity levels of chemicals

Class A – 125 to 150 fpm Class B – 80 to100 fpm Class C – 75-to80 fpm

Test method – face velocity profile and smoke generation

Scientific Equipment & Furniture Association 1028 Duchess Drive

McLean, VA 22102 (703) 538-6007www.sefalabs.com

NFPA 45 – 2002 Fire Protection for Laboratories Using

Chemicals

Laboratory hoods should not be relied on for explosion protection

Exhaust air from fume hoods should not be recirculated

Services should be external to the hood

Canopy hoods only for non-hazardousapplications

Materials of construction should have flame spread of 25 or less

80 to 120 fpm to prevent escape

NFPA 30 – 2000 Flammable and Combustible Liquids

Code

Approved cabinets may be metal or wood

Vent location on cabinets are required

Venting of cabinets not a requirement

National Fire Protection Association 1 Batterymarch Park

P.O. Box 9101

Quincy, MA 02269-9101(800)344-3555www.nfpa.org

84

Appendix E: References

General References

American Conference of Governmental Industrial

Hygienists. Industrial Ventilation, A Manual of

Recommended Practice, Cincinnati, OH

ASHRAE Standard Committee. ASHRAE Standard Atlanta:

ASHRAE Publications Sales Department, 1995

British Standards Institution, Laboratory Fume Cupboards.

Parts 1, 2 and 3, London: 1990

Department of Labor, Occupational Safety and Health Administration, 29 CFR Part 1910, Occupational Exposures to Hazardous Chemicals in Laboratories, Final Rule. Vol. 55, No. 21. Washington D.C.:1990

DiBerardinis. L. et al. Guides for Laboratory Design, Health and Safety Considerations. Wiley & Sons, 1987

McDermott, Henry, Handbook of Ventilation for Contaminant Control, 2nd Edition. Butterworth Publishers, 1985.

Miller, Brinton M. et al. Laboratory Safety: Principles and

Practices. American Society for Microbiology, Washington,

D.C.: 1986

NIH Guidelines for the Laboratory Use of Chemical

Carcinogens. NIH Publication No.81-2385.

Rayburn, Stephen R. The Foundation of Laboratory Safety,

A Guide for the Biomedical Laboratory. Springer-Verlag,

New York: 1990

Sax, N. Irving and Lewis, JR., Richard J. Rapid Guide to

Hazardous Chemicals in the Workplace. Van Nostrand

Reinhold, 1987.

Schilt, Alfred A. Perchloric Acid and Perchlorates. The G.

Frederick Smith Chemical Company, Columbus, OH: 1979.

Steere, Norman. CRC Handbook of Laboratory Safety, 2nd

Edition. CRC Press, 1971.

Collins, C.H., 1988. Laboratory Acquired Infections,

History, Incidence, Causes and Prevention, 2nd ed.,

Butterworths, London.

85

Appendix E: References

Kruse, R.H., WH. Puckett and J. H. Richardson. 1991. Biological Safety Cabinetry, Clin. Microbiol. Rev. 4:207241.

Miller, C.D., D.H.M. Groschel, J. H. Richardson, D. Vesley,

J. R. Songer, R. D. Housewright and W. E. Barkley. 1986.

Laboratory Safety, Principles and Practices, American

Society for Microbiology, Washington, D.D.

National Research Council (U.S.) Committee on Hazardous Biological Substances in the Laboratory. 1989. Biosafety in the Laboratory. Prudent Practices for Handling and Disposal of Infectious Materials. National Academy Press, Washington, D.C.

Rayburn, S.R. 1990. The Foundations of Laboratory Safety,

Springer-Verlag,New York.

U. S. Department of Health, Education and Welfare.

Formaldehyde Decontamination of Laminar Flow Biological

Safety Cabinets, National Institutes of Health, Division of

Safety, Bethesda, MD 20892.

U. S. Department of Health, Education and Welfare.

Effective use of a Laminar Flow Biological Safe Cabinet.

National Audiovisual Center (GSA), Sales Branch, Catalog

No., NAC 005133, Washington, D.C.

U. S. Department of Health, Education and Welfare. 1975.

Selecting a Biological Safety Cabinet. National Audiovisual

Center (GSA), Sales Branch, Catalog No. NAC 000709,

Washington, D.C.

U. S. Department of Health and Human Services. 1999.

Biosafety in Microbiological and Biomedical Laboratories, 4th ed. U.S. Department of Health and Human Services, Publication No. (CDC) 93-8395. U. S. Government Printing Office, Washington, D.C.

86

87